Lu Li and Wang Xiaoxiao were eating the apples that Goofy brought back in the afternoon.

Most of the apples in Lu Li's hand were eaten, and Wang Xiaoxiao joked on the side, "Others eat apples with big bites, but when you eat apples, you are gnawing, and there are tooth marks everywhere." ”

"My front teeth are a bit like rabbit teeth, so I like to nibble on apples, and you see if this half of an apple looks like it has been gnawed by a rabbit."

"It's not like a rabbit gnaw, it's more like a rat."

"Rabbits and rats are rodents, aren't they all the same?"

"It's different, rats are too dirty, especially wild rats can spread dozens of diseases, rabbits are much cleaner."

"There are no fewer types of bacteria in humans than in mice, but there are relatively fewer types of pathogenic bacteria."

The door of the emergency office was pushed open with a "creak", and Gu Yanan and Gu Qingcen appeared at the door.

Seeing that Lu Li and Wang Xiaoxiao were still in the office, Gu Yanan remembered that he had instructed the two of them here in the afternoon to prepare things for the patient's home, and now let the county CDC take samples on their behalf, so the two of them did not need to go to the scene.

Gu Yanan: "Lu Li and Xiaoxiao, you two can go back from work first." ”

Lu Li felt puzzled and asked, "Sister Yanan, don't we have to go to the patient's house overnight to do the flow survey?" ”

"No, I asked the people from Guanyun County Disease Control and Control to go to Xia Youfa's house to take samples first, they will send the specimens over overnight, and the laboratory will do pathogen testing first, if it is found, it is the best, if it is not found, we will go to Xia Youfa's house tomorrow to do a secondary investigation."

Wang Xiaoxiao was very happy, "I was going to work overtime all night just now, now it's okay, I can go back to sleep and do tomorrow's things tomorrow." ”

Wang Xiaoxiao held the half-eaten apple in his hand, and took two apples from the box and stuffed them into his bag, Lu Li was the same, after saying goodbye to Gu Yanan and Gu Qingcen, the two left the emergency office together.

Only Gu Yanan and Gu Qingcen were left in the office.

Gu Qingcen saw that Gu Yanan didn't look like he was leaving, and asked, "Are you going to wait for the results of the laboratory in the office?" ”

"No, I'm going to help in the lab, there are two colleagues in the lab who are on public leave, and there is a shortage of overtime at night, I can go and help, I hope to get the results sooner."

"Okay, I'll go with you."

——

Lianhai Center for Disease Control and Prevention, Laboratory.

Xiao Zhang, who works overtime in the laboratory, plus Gu Qingcen and Gu Yanan, are busy working in the laboratory.

Although the county hospital and the city's First People's Hospital have ruled out epidemic Japanese encephalitis, epidemic hemorrhagic fever caused by hantavirus, dengue fever, and acute jaundice viral hepatitis, Gu Yanan still did it again, and added several other pathogens common to viral hemorrhagic fever.

All negative.

At the same time as PCR, Xia Youfa's blood and cerebrospinal fluid were also cultured.

However, it takes at least 24 hours or more to culp the results, and this method is suitable for obtaining bacterial strains, but is not suitable for rapid identification of pathogens.

Gu Yanan stared at the PCR test results in deep thought, thinking about the possible types of pathogens.

The specimens brought back from the hospital have been cultured and PCR tested, the serum is used for serum blood testing, and some cerebrospinal fluid is left in the test tube.

A variety of advanced laboratory tests have been used, and the type of pathogen has not yet been identified.

Perhaps the simplest experimental method can be used to examine directly under the microscope.

Gu Yanan entered the microscope room and found the inverted microscope.

Gu Qingcen followed Gu Yanan closely, saw Gu Yanan find the microscope, and knew Gu Yanan's intentions.

In the dark field of the microscope, a specimen of cerebrospinal fluid (CSF) reveals a question-marked organism in motion.

"There are discoveries." Gu Yanan's voice was pleasantly surprised.

Gu Qingcen came over, and Gu Yanan let go of the microscope and gave Gu Qingcen a look.

"Leptospira?!" Gu Qingcen said the name of the pathogen.

"Well, from the morphological point of view, it should be leptospira, our Lianhai City is the natural foci of leptospira, there were outbreaks of cases many years ago, but in recent years, environmental sanitation and prevention and control measures have been done well, this kind of cases are getting fewer and fewer, and I didn't expect it when I was tested just now."

"Next, do PCR and serological verification experiments, and Xia Youfa's disease can be diagnosed."

While doing verification experiments in the laboratory, Guanyun County CDC sent the samples collected at Xia Youfa's home overnight.

3 a.m.

After a series of tests, leptospira was detected in Xia Youfa's blood and cerebrospinal fluid.

No leptospires were found in food, soil, water collected from Xia Youfa's home, as well as in domestic pigs and dogs.

3:20 a.m.

Lianhai Center for Disease Control and Prevention, Emergency Office.

After Gu Yanan fed back the experimental results to the People's Hospital, he and Gu Qingcen discussed the experimental results in the office.

Gu Qingcen: "Xia Youfa is sure to be infected with human leptospirosis, but no leptospirosis infection source has been found in his home, it may be that the county disease control did not take complete samples during sampling, or the source of infection infected Xia Youfa is not in his home." ”

Gu Yanan: "Lianhai City is a natural foci of leptospirosis, and there were three types of epidemic forms in the past: rice field, rain and flood, but in recent years, there have been very few cases, and people are infected with leptospirosis, usually through contact with paddy fields, rivers and lakes contaminated by infected animals, as well as muddy soil and wet grassland.

Xia Youfa's family has paddy fields, and now it is almost the rice harvest season, if his rice field is infected by Leptospira, and he has been working in the field recently, it is very likely that he will be infected. ”

"If it is a problem with the rice fields, then the residents of the entire Xiajia Village and the villages adjacent to the rice fields are at risk of being infected, and we will have to do a lot of work tomorrow."

"Yes, not only Xia Youfa's family, but also the entire Xiajia Village and nearby villages, this workload is definitely not enough with only a few people from our city's CDC emergency team, I will ask the county CDC to dispatch people to help tomorrow." It's almost 3:30 a.m., so let's rest and recuperate, there is still a lot to do tomorrow. ”

——

Day 2 at 9 a.m.

Guanyun County, Xiajia Village.

The on-site flow shunting of the city disease control stopped at the entrance of the village, and 6 people from the emergency team got off the car, and Xia Youfa's wife Li Xiaoju came with the emergency team.

Early this morning, Li Xiaoju contacted Gu Yanan through Xia Youfa's attending doctor, Gu Jiashu, and she decided to follow her husband's arrangement, since staying in the hospital was not helpful, she went home to help the people from the CDC find the source of infection.

Five people from the on-site department of Guanyun County Disease Control and Prevention and the leaders of the county CDC waited at the entrance of the village early, and after meeting with the six people from the municipal CDC emergency team, they followed Gu Yanan's arrangement and conducted the flow investigation in groups.