"I'm not sure, so it's being investigated, and the health department will inform you when the results are available." Gu Yanan told the truth.

Gu Yanan analyzed in his heart, according to the situation of the villager in front of him, he is likely to have been infected with West Nile virus before, and now he is a latent infected person or has recovered.

Coming out of the villager's house, Gu Yanan called Section Chief Li of the Laboratory Department.

"Li Ke, the physical examination specimens of the villagers in the villages near the wetland park have basically been collected, and they will be sent to the laboratory in a while, in addition to nucleic acid testing for West Nile virus, it is also necessary to check the patient's serum antibodies."

Section Chief Li on the other end of the phone was in the laboratory, and according to the results of previous high-throughput sequencing, he had designed specific primers and sent the primer sequences to a biotechnology company located in a biotechnology park on the outskirts of Lianhai City.

The biological company promised to deliver primers to the door within three hours, which means that from now on, it will be able to do a large number of nucleic acid tests for West Nile virus in three hours.

However, now Gu Yanan has asked for a serum antibody test for patients, but this kind of kit is not readily available, and it is too late for biological companies to do it.

Section Chief Li said the current difficulties, and finally added: "Because the West Nile virus rarely appears in China, and there are only a handful of imported cases reported in the literature, so these reagent manufacturers have no reserves, and it is not easy for us to make nucleic acid tests in such a short period of time, and there is really no way to do this serum antibody test." ”

"Okay, the laboratory will do a good job of nucleic acid testing first, and I'll figure out how to solve the problem of serum testing kits."

Gu Yanan thought of one of his classmates in the National Center for Disease Control and Virus Control, who had done research on West Nile virus with his mentor abroad before, and maybe he would have some reserves of serological testing reagents.

I flipped through the address book on my mobile phone, found the contact information of the classmate, dialed the phone, and explained the situation.

Fortunately, the students did have some reserves of serum testing reagents, but the number was small.

The classmate promised to send these reagents to Gu Yanan by air express for emergency, and at the same time sent the contact information of a foreign biological reagent manufacturer by text message, suggesting that she urgently order another batch from the manufacturer.

After getting the contact information, Gu Yanan sent it to the logistics department of the unit and asked them to contact it.

When Gu Yanan called to contact the serum testing reagent, the physical examination work in each village had been completed, and the collected specimens were collected and collected into the sampling box of the on-site flow shunting vehicle where Gu Yanan was located.

On the way back to the CDC from the wetland park, it was late and the sky was getting darker.

Gu Yanan was using his mobile phone to contact Section Chief Li of the laboratory about nucleic acid testing, when suddenly his mobile phone buzzed.

glanced at the phone screen and found that it was his brother Gu Jiashu calling.

Gu Yanan ended the call with Section Chief Li and connected to his brother Gu Jiashu's phone.

"Little sister," Gu Jiashu's voice was anxious, "Lao Zhang, the leader of the survey team, is dead. ”

"Huh?!" Gu Yanan felt unbelievable, when he was chatting with his brother Gu Jiashu before, he heard the news that the patient was in stable condition, why did he suddenly die?

"When did you die? How did he die? Gu Yanan asked.

"More than half an hour ago, I suddenly suffered from multiple organ failure, and I died after ineffective rescue. I know that you are busy with the flow of things, and I wanted to sort out the complete medical records and death report before telling you the news, but I didn't expect the news to have reached the Internet in such a short time. The news said that someone had died of West Nile virus infection, and the news had turned sour after adding oil and vinegar.

Now the news about this West Nile virus has exploded on the Internet.

I have analyzed that the medical staff of our infectious disease department and the family members of the patients who have access to this news are also the people who sent it. ”

The official announcement of the epidemic has not yet been released, because the ins and outs of the epidemic have not yet been clarified, and now someone has posted the news on the Internet, which will definitely cause panic among the people.

"Brother, I know, I'll take care of this matter."

Before he could end the call with his brother Gu Jiashu, there was another buzzing sound on the mobile phone, and the caller ID was Director Yang, the director of the center.

Connected to the phone, as Gu Yanan expected, it was indeed about the West Nile virus rumors on the Internet, "Xiaogu, have you seen the information on the Internet?" What do you think? City leaders and health authorities have called me and asked for a quick resolution of the matter. ”

The best way to quell the rumors is to reveal the truth.

Gu Yanan: "Director Yang, I just contacted Section Chief Li of the laboratory, the patient's nucleic acid test report has come out, and I will first draft an epidemic report and send it to you." ”

"What about the villagers in the wetland park and nearby villages?"

"The mosquito trap has been installed, the mosquito samples need to be collected tomorrow morning, the villagers' samples have been collected, and the villagers' nucleic acid test results can be obtained tonight.

The results of the survey of mosquitoes in the wetland park will be available at noon tomorrow, and whether a natural foci of the West Nile virus have been formed in the wetland park will be determined at noon tomorrow.

However, as for the reasons for the formation of natural foci, it is necessary to continue to investigate. ”

"Okay, let me know as soon as the result comes."

——

7:40 a.m. the next day.

Lianhai Center for Disease Control and Prevention, Experimental Building.

After working overtime all night, the results of the experiment finally came out.

Some villagers in villages near the wetland park have tested positive for West Nile virus nucleic acid.

The positivity rate is 3%.

Through telephone inquiries about epidemiological history, it was found that these people who tested positive for nucleic acid had recently visited the wetland park.

Moreover, these people have no obvious symptoms and are insidiously infected.

9:30 a.m.

Mosquitoes collected from the wetland park are sent to the laboratory one after another.

The experimenters were tested in batches according to the mosquitoes collected at different locations in the wetland park.

12:10 p.m.

The nucleic acid test results of mosquitoes are out.

West Nile virus nucleic acid positive was detected in mosquitoes at sampling sites around several lakes.

The test positivity rate is close to 20%.

Gu Yanan analyzes the experimental results.

West Nile virus has been detected in dead birds found in wetland parks.

The West Nile virus has been detected in mosquitoes and residents of nearby villages.

West Nile virus has also been detected in horses, livestock in nearby villages.

Mosquito-bird-mosquito, the chain of transmission in which the West Nile virus circulates in nature exists.

The presence of the new Nile virus was also detected in terminal hosts such as humans and horses.

All evidence supports the formation of a natural foci of the West Nile virus in the Crescent Bay Wetland Park.