Gu Yanan: "I used a similar model when I was doing a postdoctoral program before, and I saw the engineer's installation process in the morning, and I also read the manual, so it's no problem to use." ”

Xiao Chu immediately looked at Gu Yanan with adoring eyes.

The technology to be used now theoretically belongs to viral metagenomics technology, which is to analyze the viral genome in sequencing data by bioinformatics methods through high-throughput sequencing.

The pathogen genes can be found through analysis, and then according to the results of high-throughput sequencing screening, primers are designed, and qPCR quantitative amplification is performed on the sample to further confirm the presence of viruses in the sample.

"Sister Yanan, I'm with you, and I also want to learn this new technology."

"Good. After Zhou Lao and Gao Fei came back, let them follow up on the situation of the survey team members first. Lu Li and Wang Xiaoxiao will also return from the training tonight, and let them follow up on the progress of the wetland park project, which should be suspended until the pathogen is identified and the biosafety hazard is eliminated. ”

——

Day 2 at 6:30 a.m.

The sun had just risen, and there were only a few sanitation workers sweeping the streets.

In the experimental building of the Lianhai City Center for Disease Control and Prevention, the lights that had been on all night had just been turned off.

Gu Yanan, Xiao Chu, Section Chief Li of the Laboratory Department, and several inspectors were busy in the testing center all night and finally got the results.

By analyzing the viral ome in the high-throughput sequencing data, it was found that the causative virus in the patient's body turned out to be West Nile virus.

Everyone looked at the result and fell silent for a while.

"This result is reported, no fryer!" Section Chief Li sighed.

West Nile virus belongs to the genus Flavivirus in the Flaviviridae family, which belongs to the same family of viruses as Japanese encephalitis virus and dengue virus, and can cause symptoms of encephalitis.

In nature, it circulates in a mosquito-bird-mosquito cycle, with birds as storage hosts and domestic animals such as humans and horses as terminal hosts.

Another characteristic of West Nile virus is that it has so far only been endemic in Africa, the Americas, Europe and North Asia, and there have been no indigenous cases in China.

Xiao Chu: "When I was conducting a survey for the survey team, I specifically asked them about their recent experiences, and I determined that they had not left Lianhai City in the past three months, let alone gone abroad. ”

Section Chief Li: "In the past, when the plague epidemic occurred, it was controlled at home and abroad for a period of time, and now there are very few people who travel abroad, and they have not been to foreign epidemic areas, which is a local case." ”

"Local cases?!" Several inspectors in the laboratory department exclaimed at the same time.

If the case is autochthonous, it means that there is a natural host and vector of the new Nile virus in the environment surrounding these cases, i.e., there is a natural foci.

Everyone understood the seriousness of the matter, and their eyes all looked at Gu Yanan.

As the director of the emergency office, Gu Yanan is organizing and coordinating when encountering major epidemics.

She understands that the first priority now is to find out whether the West Nile virus has a natural foci in Lianhai City.

Since the infected survey team members only showed symptoms of infection after the survey of the Crescent Bay Wetland Park, the most likely foci of the epidemic is the Crescent Bay Wetland Ecological Park, and the next step is to investigate whether the residents near the wetland park are infected by the West Nile virus.

Gu Yanan began to assign tasks to everyone.

"Section Chief Li, the laboratory department is first responsible for designing primers based on the high-throughput test results, amplifying the gene fragments of West Nile virus from patient specimens, and further determining the presence of the virus.

The CDC and I went to the Crescent Bay Wetland Park to catch mosquitoes and wild birds, and then conducted surveys in nearby villages to see if the residents were infected, as well as the situation of domestic animals in the villages, to determine whether there were natural foci. ”

——

7:30 a.m.

Lianhai Center for Disease Control and Prevention, Emergency Office.

Although there was still an hour before work, several other members of the emergency response team were urgently called back by Gu Yanan.

In the office, Gu Yanan briefly introduced the current situation.

Wang Xiaoxiao had just arrived at the office, still carrying half a cup of unfinished soy milk in her hand, and when she heard the explosive news announced by Gu Yanan, she stood in place in shock, "There may be a natural foci of the West Nile virus?" …… It's horrible. ”

Lu Li: "It's still uncertain, so our emergency team has to rush out to investigate." ”

Gao Fei: "I need to go to the wetland park to catch mosquitoes, I heard that the wetland park is very large, and a few of our emergency response team have to go to the villages near the wetland park to investigate, and there is a serious shortage of people to catch mosquitoes." ”

Zhou Lao: "The mosquito catcher can be seconded from the coastal area CDC, but I am more worried about how there is a natural foci of the West Nile virus. The virus can't appear out of thin air, it must have come in some form from somewhere, and this introduction process must also be investigated, but it is estimated that it is difficult to check. ”

Gu Yanan frowned slightly, she was worried about what Zhou Lao was worried about now as soon as she found out that it might be a cluster outbreak of West Nile virus infection.

Gu Yanan: "This problem that Elder Zhou said is indeed to pay attention to, but at present, we will first divide into groups to catch mosquitoes and conduct a survey of the village.

Zhou Lao and Gao Fei went to coordinate with the people of the coastal area disease control to place mosquito traps on the entire Crescent Bay Wetland Park.

Lu Li, Xiao Chu and I went to the village near the wetland park.

Wang Xiaoxiao stayed in the office to find out the imported West Nile virus infection cases in Lianhai City in the past three years, and then contacted the National Disease Control and Prevention to find information on imported West Nile River infection cases nationwide through the epidemic system to see if these cases had passed through or stayed in Lianhai City before the onset of the disease. ”

"Understood!"

The members of the group spoke in unison and began to split up.

——

Lianhai City, Binhai District, Crescent Bay Wetland Park.

Zhou Lao and Gao Fei contacted the staff of the coastal area disease control and divided the entire wetland into districts, and each person began to plant mosquito traps in different areas.

Because they know that mosquitoes and birds in the wetland park can be deadly, the disease control staff are fully armed to prevent mosquito bites, which is summer, and the protective clothing on their bodies is very stuffy.

Zhou Lao also carried the warning signs prepared by the health education department and placed them at regular intervals on the periphery of the wetland park.

In addition to setting up mosquito traps to catch mosquitoes, there are also many bird carcasses in the wetland park, and Zhou Lao led the disease control team to collect bird carcasses while placing mosquito traps to prevent people from straying into the wetland and contacting dead birds and being bitten by mosquitoes to cause the spread of the epidemic.

Several people from the district disease control and control were able to work hard, but Gao Fei asked Zhou Lao, "Zhou Lao, the transmission of the West Nile virus is mosquito bites, as long as people are not bitten by mosquitoes, it doesn't matter if they come into direct contact with wild birds, so why are these wild bird carcasses collected?" ”

"Birds are an important part of the West Nile virus cycle in nature, and it's always good to remove some of the dead birds from this ring. Besides, in addition to the West Nile virus, there may be other pathogens in the bodies of these dead birds, so it is not a problem to make these dead birds stink here. ”