Pang Xuelin paused and continued: "When the virus leaked in the hive laboratory, I was in a hurry, and I directly injected the samples of the new virus into my body, and I didn't have time to copy the relevant gene sequences of the new virus from the hive laboratory, so now it is more difficult to redevelop that virus, and there is another problem......

"What's the problem?"

"The Kempten base laboratory is relatively rudimentary, and we lack some high-end experimental equipment and equipment. For example, if we want to determine the dynamic characteristics of proteins, we need dynamic APT, which is called a dynamic atom probe chromatograph, through which we can observe the dynamic characteristics of protein molecules and RNA molecules. ”

Albert pondered for a moment and said, "You make a list of the supplies you need, and I will send someone to help you get all the equipment." ”

Pang Xuelin showed a satisfied smile on his face and said, "Mr. Albert, then I'll trouble you." ”

Next, Pang Xuelin cooperated with the researchers brought by Albert Wesker and let them collect relevant blood and biological samples from himself.

He even called Alice and asked her to cooperate with the umbrella company to collect samples.

Now because of the appearance of Pang Xuelin, Alice is no longer the focus of the umbrella company, and Albert has not expressed any opinion on Alice's appearance at the Kempten base.

When all this work was done, Albert Wesker politely declined Pang Xuelin's invitation to dinner and hurriedly took the plane to leave.

Looking at the helicopter of the umbrella company in the distance, Pang Xuelin said to Alice who was following him: "Alice, get ready, after the equipment of the umbrella company is in place, you will go to the Hill military base." ”

"Good!"

Alice nodded.

Two days later, Umbrella sent a dynamic atom probe chromatograph, as well as a variety of cutting-edge experimental instruments and consumables.

Spruce, Berkeley, Huang Yu and others were dumbfounded, many of the experimental equipment in it were unique to the umbrella company, and the outside world had never seen them at all.

Even Pang Xuelin looked around the dynamic atom probe chromatography for a long time, if this thing is put in modern society, it can be regarded as a big killer for biology and materials science research.

It's just a pity that he is now mainly focused on researching how to fight the T virus, and he doesn't have time to learn APT technology at all.

A week later, Alice led an elite team of more than 200 people to sweep the Hill base at the cost of more than 20 people, and obtained a large amount of weapons and ammunition from the Hill base.

In addition to rifles, machine guns, armored vehicles, and various artillery pieces, Alice even brought back three tiltrotor helicopters.

In this way, the external long-distance transportation of the Kempten base no longer depends only on the fleet of the umbrella company, which is of great significance for the future development of the Kempten base.

In addition, Alice did not disappoint Pang Xuelin, and actually got three tactical nuclear warheads at Hill Air Force Base.

However, Alice did not return the warheads to Kempten, but hid them in a valley twenty kilometers away from Kempten.

Only Pang Xuelin, Alice, Claire and Jill know about this matter, these three nuclear warheads have a small yield, only equivalent to the power of 20,000 tons of TNT, and the weight of a single warhead is only about 50 kilograms.

Pang Xuelin carried two nuclear bombs and could walk as fast as he could in the mountains.

The secret bases of the umbrella company are basically underground, if nothing can be done, or if Spencer puts pressure on the Kempten base after realizing that Pang Xuelin has been perfunctory, these three nuclear warheads are enough for Pang Xuelin to destroy Spencer's underground base.

Late at night, after confirming that the three nuclear bombs were hidden, Pang Xuelin and Alice quietly returned to the Kempten base.

"Okay, it's not too early, you can go back and rest early."

Pang Xuelin was about to say goodbye to Alice and go back to his room to rest.

Alice suddenly said in a loud voice: "Wait......"

"What's wrong?"

Pang Xuelin turned around and looked at Alice.

Alice said, "Pang, when will we be able to attack the Umbrella Company?" ”

Pang Xuelin was slightly stunned and said, "Didn't you say okay, wait until I develop an antidote to the T virus, and then I will do it?" ”

Alice shook her head and said, "I'm afraid I won't be able to wait that long. ”

"Why?"

Pang Xuelin frowned.

Alice said: "Spencer wants to destroy all mankind to establish his own super empire, and now it has been nearly a year since the outbreak of the T-virus, and the total number of surviving humans in the world at present is not necessarily 10 million, and in a few years, I'm afraid that even if we kill Spencer and them, there will probably not be a few humans left on the earth." ”

Pang Xuelin said: "Then what are you going to do?" ”

Alice said: "In a year, within a year, if you can get the antidote to the T virus, then we will continue to work together, if you can't do it, I will bring a nuclear bomb to talk to Spencer." ”

Pang Xuelin looked at Alice quietly, and he could feel the other party's determination.

Pang Xuelin pondered for a moment and said, "Okay, then I'll do my best." ”

Alice just nodded faintly, stopped talking, and turned to leave.

Pang Xuelin looked at Alice's back, a little helpless.

Although he fooled Alice to the Kempten base, and the two sides got along for half a year, he could feel that Alice did not fully trust him.

But this is also something that can't be helped, Pang Xuelin is still in conflict with the umbrella company.

If Spencer hadn't been more interested in the genetic optimizer in Pang Xuelin's body, and hadn't attacked Alice again, I'm afraid Alice would have gone berserk a long time ago.

……

Alice's words put a certain amount of pressure on Pang Xuelin, and in the following time, he focused all his energy on the research of the antidote to the T virus.

Now, it was the turn of Spruce, Ashford, and Berkeley to be shocked.

Although Pang Xuelin had followed them to study the T virus before, he played the role of an assistant most of the time, and rarely took the initiative to express his personal opinions on academic issues.

But when he really started to lead the operation of the laboratory, he quickly showed the strength of an academic master.

There are more than 30 people in the laboratory, except for Pang Xuelin, Spruce, Berkeley, Huang Yu, and Ashford, the rest are doctoral students or research assistants with certain academic backgrounds like Xiao Dongbei.

In the first three months of the study.

Pang Xuelin first asked Xiao Dongbei to analyze the structure and various data of various proteins and non-coding RNAs in cells through cryo-electron microscopy, atomic probe chromatography, nuclear magnetic resonance instrument and other equipment.

After obtaining these data, Pang Xuelin began to establish the kinetic and biological function models of the structure and dynamic characteristics of proteins and non-coding RNAs through MOSS-controlled mathematical methods, and asked MOSS to write simulation software for proteins and non-coding RNAs.

This work is very tedious and complicated, but Pang Xuelin insisted on relying on his strong mathematical skills and the super computing power of quantum computers to complete the software writing work.

When he applied this software to his actual work, he instantly calmed down the Sprus.

With this software, scientists can actively construct some new RNA molecules and protein molecules, and then use the software to simulate the biological functions of these molecules.

As a result, they soon discovered that the software not only simulated with great accuracy, but could even predict the existence of undiscovered protein molecules and RNA molecules.

Even, Pang Xuelin used this software to build a biodynamic model of the T virus and improve the picture of the T virus invading the human body.

First, the T virus binds to the specific receptor of the host cell through its surface glycoprotein, and then the viral envelope fuses with the cytoplasmic membrane (i.e., through the PH-independent pathway) or the endosomal membrane with an acidic environment (PH-dependent pathway) to release the viral ribonucleoprotein complex (RNP) into the cytoplasm, where each mRNA is synthesized during transcription, and the full-length antisense genomic RNA is produced through replication, which serves as a template for the viral genomic RNA.

The newly synthesized RNP complex assembles with viral structural proteins in the cell serous membrane or Golgi membrane, and then releases the newly synthesized progeny virus.

The progeny virus grows and multiplies inside the cell and then launches a large-scale attack on the surrounding cells.

Moreover, the T virus also has a miraculous property, its RNA in the body can not only use the normal cells of the human body to reproduce, but also can form a linkage effect with some non-coding RNAs in the human body, and change the growth and metabolism rate of human cells on a large scale.

What Pang Xuelin wants to do is to find a non-coding RNA that can interfere with the RNA of the T virus, so that the T virus will fall into a silent state, and even if it infects the human body, it will not be able to reproduce in a large range of the human body because the genes cannot be expressed.

……

Time passed day by day, and before you knew it, half a year was fleeting.

On this day, Pang Xuelin had just come to the laboratory, and Sprus, who was on duty, found Pang Xuelin excitedly and laughed: "Professor Pang, have we found the target RNA?" ”

"Found it, what group is it?"

Pang Xuelin's eyes lit up, and he said with some surprise.

Previously, using the protein and RNA molecular biodynamics function simulation software developed by Pang Xuelin, Pang Xuelin and they wrote a special RNA with the ability to silence the T virus gene.

Subsequently, they screened thousands of RNA species in human cells and found more than 200 RNAs.

Next, it's a group of experiments.

It's a tedious job, and it's basically a matter of repeating the previous work over and over again.

Pang Xuelin did not take the initiative to participate, he had already planned the follow-up research route, just waiting for the day when he found the target RNA.

It's just that during this time, the lab has experimented with hundreds of non-coding RNAs, but they have never been able to achieve the desired results.

Pang Xuelin wondered if he had taken the wrong path before, if the appointed time of the year was up, and Alice really wanted to leave Kempten Town and embark on the road of direct confrontation with the Umbrella Company, then Pang Xuelin really had no good way to stop the other party.

Spruce said: "It was group 112, and after we injected this RNA-active extract into the cells, we observed a significant decrease in the amount of T virus in the cells through dynamic APT, and even stopped the synthesis of the RNP complex. ”

Pang Xuelin nodded and smiled: "This is good news, has it been observed how the No. 112 non-coding RNA achieves the mechanism of gene silencing of T virus RNA?" ”

Spruce said: "We found that after RNA 112 enters the cell, through the intracellular RNA-protein linkage, the lysine residues of the histone N-terminal domain can be deacetylated and methylated, which is catalyzed by methyltransferases, and the modification can be monovalent, divalent and trivalent methylation. T viral RNA can form heterochromatin with methylated histone-bound proteins. In addition to the N-terminal tail domain of some histones that need to be deacetylated and methylated, sometimes acetylation is sometimes required on the lysine or arginine residues of other histone N-terminal tail domains, and the final result of this modification is that the gene of the corresponding segment of the T virus is silent and loses transcriptional activity......"

Pang Xuelin pondered for a moment and said, "Bring Dr. Ashford, Professor Huang Yu, and Professor Berkeley together, and we will hold a closed-door meeting to study the follow-up work plan." ”

"Good!"

Spruce nodded.

Finding the RNA of interest is just the starting point, and there is more important work to be done.

What Pang Xuelin wants is not only to make humans immune to the T virus, what he wants to do is how to use this target RNA to create a new airborne virus, which is basically equivalent to a harmless endogenous retrovirus in the human body, but in the dead body and in the body of the T virus infected, it can instantly inactivate the T virus in their bodies.

In this way, when it comes time to deal with Spencer and his subordinates, they will lose all kinds of superpowers given by the T virus, and Spencer will be nothing more than a group of local chickens and dogs in front of Pang Xuelin.

This kind of work requires the collective efforts of Ashford, Berkeley, and Huang Yu.

Sure enough, after learning that the target RNA had been found, Ashford and Berkeley both showed joy on their faces.

The discovery of the target RNA gave them a glimmer of hope in this dark era.

However, assembling a new virus using target RNA is also not a simple task, and Pang Xuelin discussed with Sprus, Ashford, Berkeley, and Huang Yu for a whole week before completing the setting of the target virus.

Next, they combined this RNA with an adenovirus (AAV, which is harmless to humans, is found in many people, and scientists often use adenovirus to make vaccines), and then used new gene-editing tools to add some genes to the adenovirus, making the new virus airborne.

At this time, there was less than a month left before the agreement between Pang Xuelin and Alice.

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