Although it is much smaller than the Hive Lab, compared to the life science labs in the real world, the underground laboratory at the Kempten site is still a behemoth.

In the laboratory, there are spectrometers, chromatographs, gene introduction instruments, protein purification systems, cell fusion instruments, electrophoresis instruments, atomic absorption spectrometers, viral immunofluorescence analyzers, chromatographs, biochemical analyzers, cryo-electron microscopes, etc., which are common in life science research, and there are also many instruments and equipment that Pang Xuelin has never seen.

For example, the artificial uterus used for somatic cell cloning, the surface of the artificial uterus looks like a huge glass jar, connected by various pipes, which can provide a series of nutrients and growth hormones for the clones inside.

According to Sprus, this artificial womb could allow a clone to grow from a fertilized egg to an adult state in half a year.

Pang Xuelin suddenly realized that the glass jars filled with Alice's clones he had seen in the underground base of Erwin Town before were probably the so-called artificial wombs.

In addition, there are some instruments that are very interesting.

For example, a 4D-E high-resolution cryo-EM microscope is used to resolve the three-dimensional structure of proteins.

This kind of cryo-electron microscope, Pang Xuelin is also the first time to see it.

In the real world, cryo-EM can be divided into three main categories, namely cryo-transmission electron microscopy, cryo-scanning electron microscopy, and cryo-etch electron microscopy.

Cryo-TEM microscopy is the most common, and a sample freezer is usually added to a normal TEM to cool the sample to liquid nitrogen temperatures. It is used to observe temperature-sensitive samples such as proteins and biological sections. By freezing the sample, the damage of the electron beam to the sample can be reduced, and the deformation of the sample can be reduced, so as to obtain the true sample topography.

Cryo-SEM is a fast, reliable and effective way to overcome the problem of water content in the sample. This technique is also widely used to observe "difficult" samples, such as those that are sensitive to electron beams and are unstable.

The third kind of cryo-etching electron microscope can make the sample freeze to make its microstructure close to the living state, and the microstructure of different cleavage surfaces can be observed after the sample is frozen fracture etching, and then the membrane structure and inclusion structure in the cell can be studied.

In the real world, cryo-EM is considered high-end equipment in the field of biology, with a price of about 6 million US dollars, and there are not many units in China, among which the largest cryo-EM center is located in the Southern University of Science and Technology in Shenzhen.

But in Kempten's underground laboratory, these three cryo-EMs have disappeared and have been replaced by 4D-E cryo-EMs.

This cryo-EM does not exist in the real world, and it uses femtosecond lasers instead of ordinary electrons to produce ultrafast images, resulting in a significant increase in sample resolution.

Moreover, after Sprus's introduction, Pang Xuelin found that the emergence of this electron microscopy technology has allowed scientists to analyze the evolution route of the three-dimensional structure and function of some protein molecules.

This is an important academic achievement.

According to the theory of evolution, a large number of structurally diverse collections of proteins in nature have evolved over a long period of time from a limited number of protein ancestors.

However, scientists have found in their research that protein molecules with new functions can evolve in a short period of time in nature.

For example, not long after the emergence of some man-made chemicals, enzymes that specialized in breaking it down appeared in nature.

Dozens of months after some new antibiotics were introduced, new proteins with drug-resistant properties appeared in some microorganisms.

The basis of the structural and functional evolution of proteins is the alteration of amino acid sequences.

The most likely sequence changes in evolution are typical mutational events such as changes in the type of individual amino acid residues at specific locations, insertion of new sequence fragments, or loss of sequence fragments.

In the real world, due to the relatively limited means of studying protein structure and observing evolution, it is difficult for scientists to clarify the physical and chemical mechanisms behind the changes in protein structure and function.

In the Resident Evil world, with the help of 4D-E cryo-EM and atomic probe chromatography, scientists have been able to clearly understand the evolution of certain protein molecules.

In addition, Pang Xuelin also saw a new gene editing technology in the laboratory.

In the real world, there are two main types of gene editing technology, one is RISPR/as9 technology and the other is single base editing technology.

The so-called RISPR, in fact, is a repeat sequence in the genome of prokaryotes, which is an immune weapon produced by the struggle between bacteria and viruses in the history of life evolution.

In order to remove the foreign invasion genes of the virus, bacteria have evolved the RISPR/as9 system, which allows bacteria to quietly remove viral genes from their own genome, which is unique to bacteria's immune system.

Microbiologists have learned that bacteria have a variety of immune functions to excise foreign viral genes, and the typical pattern is to rely on a complex that can be guided by a piece of RNA to find a target DNA sequence and then excise that sequence.

Many bacterial immune complexes are relatively complex, and scientists have mastered the technique of manipulating one protein, as9, and successively excising the DNA of multiple target cells.

This technology, known as the RISPR/as9 gene editing system, has become the hottest technology in the life sciences in the real world.

However, in practical application, RISPR/as9 technology has off-target effects, and there are serious side effects in the application of gene therapy.

However, BE3 single-base gene editing technology also has off-target problems, which can even lead to a large number of oncogene and tumor suppressor gene mutations, and also has great safety risks in practical applications.

This is also an important reason why He Jiankui, an associate professor at SUSTech, was severely condemned for his "gene-edited baby" experiment two years ago, and it is also a starting point for banning the genetic manipulation of human embryos for reproductive purposes.

Because of this, in the real world, human gene editing technology is still in its infancy.

Mankind has also been looking for a new generation of gene editing tools that are better than RISPR/as9.

In 206, there was a Han Chunyu incident in China that caused a sensation around the world.

Han Chunyu, an associate professor at HB University of Science and Technology, claims to have found a new gene editing tool, the NgAg/gDNA system, which is much more accurate than the RISPR/as9 system in terms of accuracy and editing efficiency.

After this paper was published in the top international journal Nature Biotechnology, it attracted strong attention at home and abroad, and was even praised as a "Nobel Prize-level" experimental achievement by some media.

Shortly thereafter, however, the content of the paper was mired in controversy that would be difficult to repeat.

Because biological experiments are indeed not as repetitive as physical or chemical experiments due to the operation, environment, complexity of experimental subjects, etc. For example, it is difficult to guarantee success every time for PR on complex templates, and experiments on some cells are greatly affected by cell status, cell source, etc.

Even in many NS-level articles, there will be many experimental results that cannot be repeated.

In the end, Han Chunyu's incident came to an end.

Now, Pang Xuelin has seen a new gene editing technology in the underground laboratory of Kempten Town.

This technique is called a heDNA editing tool.

This tool achieves an accuracy of more than 99.99999% when performing gene splicing, gene knock-in and knock-out, and there is almost no problem of off-target or mutation.

It is precisely because of the existence of this editing technology that the life science and technology of the Resident Evil world is so amazing.

This is also a big reason why Umbrella was able to develop the T virus.

"Professor Berkeley, where is the research on the T virus in the laboratory now?"

Pang Xuelin is very interested in the principle of the T virus.

Berkeley pondered for a moment and said, "Mr. Pang, we can basically determine now that the principle of the T virus is to change the regenerative nature of cells, so that cells can speed up their metabolism and repair ability, which also makes it difficult for many zombies to be killed by physical attacks. When humans are infected with the T virus, they are thwarted by the body's immune system. White blood cells try to kill infected cells, which causes a large amount of necrotic tissue in the body. In order to repair the loss of tissues and restore the function of the immune system, a large amount of food is needed to provide materials and energy sources, so the infected person has an exuberant appetite.

These creatures have a great appetite, as long as they see, hear, and smell the existence of creatures, they will immediately rush over and chase down the prey they are targeting, which is why zombies become so violent after being infected with the T virus.

It should be noted that under normal circumstances, the brain is protected by tissue isolation called the blood-brain barrier and does not come into contact with immune cells, so if the barrier is not damaged, the brain is likely to complete the mutation relatively intact. As a result, zombies are still able to walk upright and forage for food. That's why we shoot zombies in the brains before they die completely......"

Pang Xuelin said: "Then how long do you think this crisis will last before it ends?" Will those zombies die in the future? ”

Berkeley said: "Zombie activities also need energy, and more than 99% of the world's people have been infected with zombies, which has led to an increasing lack of food for zombies in cities. If you don't get food for a long time, the zombies can only maintain their basic vitality by corroding the body, according to our estimates, the fastest five years, the slow ten years, the world's zombies will die on a large scale, but at that time, how many people are left on the earth, it is unknown. ”

Pang Xuelin nodded and didn't ask any more.

It was much the same as he had guessed.

For those zombies who have already been infected, the best thing to do is to wait for them to slowly die.

What really trickled Pang Xuelin was Spencer, Mascu, William Birkin, and other careerists from the umbrella company.

Pang Xuelin could vaguely guess Spencer's thoughts.

For Spencer, after being infected with the T virus or G virus, they have become monsters in the eyes of ordinary humans.

In a sense, they have even become other species.

This is also the reason why Spencer and they were able to launch a Resident Evil on Earth without any scruples.

The extinction of mankind, in the eyes of these people, is nothing more than the extinction of a foreign race.

What they really want to do is lead the people at Umbrella Inc. to find a completely new, flawless path of evolution.

If they succeed, another human-like species will be born on Earth in the future.

They will also become the progenitors of this species, becoming gods in the truest sense of the word.

And Pang Xuelin wants to preserve humanity, and sooner or later he will have to go to the opposite side with Spencer.

Fortunately, Pang Xuelin still has time to buffer, and the body modified by genetic optimizers has made Spencer recognize Pang Xuelin's existence.

Therefore, as long as they do not make progress in the research of T/G virus, then they will not easily attack Pang Xuelin.

"We must find a way to find an antidote to the T virus, and only Spencer and his men can fight back without the blessing of the T virus."

After visiting the underground laboratory.

Pang Xuelin was about to say something to Sprus, who was following him, when he saw Jill hurriedly come over and said loudly: "Professor Pang, it's not good, the Umbrella Company sent Dr. Ashford here, Alice wants to kill him, Claire is trying to stop Alice now." ”

Pang Xuelin was slightly stunned and said, "Let's go, let's go up and have a look." ”

Back on the ground, Pang Xuelin saw Alexander Ashford sitting in a wheelchair at the gate of the manor, Alice holding a knife to Dr. Ashford's neck, and the soldier sent by the Umbrella Company to protect Ashford pointed a gun at Alice, and Claire was trying to persuade the two. ”

"What are you doing here?"

Pang Xuelin stepped forward and grabbed Alice's long knife directly.

Alice struggled slightly, as if she was worried about hurting Pang Xuelin, so she didn't use much force.

She glared at Pang Xuelin and said, "This guy is not a good person, why are you protecting him?" ”

Pang Xuelin's hand was slightly forced, snatched the knife from Alice's hand, and said with a smile: "Dr. Ashford is his own person." ”

As he spoke, Pang Xuelin winked at Alice.

Alice frowned, looked at Pang Xuelin, and said after a while: "Remember what you told me some time ago. ”

With that, she turned and left.

Pang Xuelin turned his head, looked at Ashford and said, "Dr. Ashford, I'm sorry, my companion's temper is relatively hot. ”

Ashford waved his hand, motioned for his men to put their guns down, and said, "It's okay, Professor Pang, it's nice to see you again." ”

Pang Xuelin smiled: "I'm also very happy to see you again, by the way, doctor, what about Angela?" Why didn't she come? ”

For that smart little girl, Pang Xuelin has a good impression.

Ashford showed a wry smile on his face and said, "Mr. Spencer was worried that Angela would not adapt to life here, so he didn't let her come." ”

Pang Xuelin was slightly stunned, nodded and said, "I see." ”