On Tuesday, Qi Min finally went to the laboratory on time. Although she is not actually that dedicated, it would not be good if Zhang Zhiyong's people arrived and found that she was not there.

And since Qi Min arrived at the laboratory, he followed Ke Li to prepare the rats. Fluorescent labeling is to be labeled on nerve cells connected to electrodes near the corpus callosum of rats, so rats still need to be surgically surgified. In fact, it is easy to get infected after such frequent surgeries, so it is necessary to carefully disinfect the vicinity of the rat's wound.

However, this time, Qi Min also tried peptide quantum dots for fluorescent labeling, which was still an experimental exploration, so instead of using a rat that had been connected to the callosum interface, a new rat was used. She and Ke had to first make a wound on the head of the rat that mimicked the interface connecting the corpus callosum, and then fluorescently label the nerve cells through this wound.

Just as Qi Min was rubbing the skin of the rat's head with an alcohol cotton, suddenly the mobile phone in his pocket rang.

Most of them are peptide quantum dots. Qi Min said hello to Ke Li, then walked out of the clean room and took off his gloves, took out his mobile phone and saw that it was indeed Zhang Zhiyong: "Hey, how is it?" ”

"I'm all set, downstairs in your department." Zhang Zhiyong said, "Where is your laboratory?" ”

Qi Min didn't expect him to send it over personally, and said a little embarrassedly: "I'm in the ultra-clean room on the third floor now, or ...... You're just going to come up? There's a sterilized lab coat here......"

"You're doing rats right now? Looks like I've come just in time. Zhang Zhiyong smiled.

Qi Min hung up the phone, changed his head cover, gloves, masks, and shoe covers, and then sprayed 75% ethanol for disinfection, and then re-entered the ultra-clean room after passing through the air shower room.

Ke Li asked while opening the skull of the rat, "The peptide quantum dots have arrived?" ”

"It's coming soon." Qi Min said with some shame, "Why don't you ...... Let's use your rat, I'm not done yet. ”

Ke Li knew that she had slow hands, and this was already a routine operation for them to cooperate with the experiment. It's just that I pity the rat that was assigned to Qi Min's operation, and after anesthesia, he had to lie in the safety cabinet and blow cold air for so long.

Qi Min watched Ke Li quickly complete the fake operation on this rat, and then after a while, he heard the sound of the preparation room outside the ultraclean room opening the door. After a burst of squirming with shoes and gloves, the air shower room opened, and it was another blow, and then the "fully armed" Zhang Zhiyong carried an ice box into the clean room.

As soon as he came in, he looked around for a week and teased: "Hey, the laboratory of the biology department is so clean. ”

"This is a clean room for animal experiments, and the laboratory is not so tidy." Qi Min smiled and explained.

"It's a hassle to get in here." Zhang Zhiyong put the ice box on the central laboratory table, and took out a 15mL centrifuge tube wrapped in aluminum foil from it, "Peptide quantum dot solution, protect it from light at 4 °C." Where is your refrigerator? ”

"Our lab is downstairs...... Why don't you put it in an ice box first? Qi Min said with some embarrassment.

"That's fine." Zhang Zhiyong took out another 1.5mL small centrifuge tube, "Can you operate this mouse?" ”

Qi Min glanced at Ke Li, who was a little inexplicable about the person who began to shout as soon as he came in, and turned his head to Zhang Zhiyong and said, "The wound has been done." Need to be cleaned? ”

Zhang Zhiyong looked at Ke Li, who was sitting in front of the safe, and said to Qi Min: "I don't know the operation of your creatures. But am I teaching him, or am I teaching you directly? ”

Qi Min also forgot to introduce Ke Li to Zhang Zhiyong at first, but he knew with his toes that the people who did the experiments with Qi Min were either people from her laboratory or people from the collaborator's laboratory, in short, they were all her subordinates.

Zhang Zhiyong, like most of the PIs in the school with some qualifications, has a superior-to-subordinate attitude towards his doctoral students and technicians. But Qi Min is usually much more cordial to Ke Li, and he doesn't really want Ke Li to be instructed by Zhang Zhiyong, so he said: "You better teach me directly." ”

Ke Li inexplicably got up from the chair, changed Qi Min to sit over, disinfected gloves with 75% alcohol, and then reached into the safe.

And she had done the experiment so personally, Zhang Zhiyong was embarrassed to stand on the side and command, so he also found a chair to sit on.

"You'd better spray some alcohol." Qi Min joked to him, "Actually, if you don't pass the experimental safety exam of the biology department, you can't enter the ultra-clean room." ”

"The stipulation is dead, but man is alive. Could it be that the head of your biology department won't let me in? Zhang Zhiyong also jokingly answered, and then said, "You put this mouse ...... Brain, rinse it out. It's kind of disgusting to look at it that way. ”

In fact, the exposed part of the safe cabinet is not the cerebral cortical tissue of the rat, but only the skull. The corpus callosum interface is minimally invasive and needs to be inserted through a needle. However, Qi Min still rinsed the wound with normal saline.

"It doesn't feel right...... The brain shouldn't be so smooth. Zhang Zhiyong said again.

"It's a skull, and the fluorescent substance has to be driven through the pinhole." Qi Min explained.

"Then what are you doing at it?" Zhang Zhiyong immediately said, "My God, you washed the surface with water, and the pinholes are now full of water, aren't the peptide quantum dots diluted?" ”

Qi Min was helpless: "You made me rush." ”

"Who is the ...... of biology" Zhang Zhiyong was also helpless, "Why are you so adult, and you are still unreliable-do you have to think more, do you want to think more?" ”

He said two "think more" in a row, Qi Min vaguely felt that there was a hole in it, but he still agreed: "Yes, yes." But now it's diluted, and there's no way around it. ”

"Why can't you do it?" Zhang Zhiyong said, "Peptide quantum dots should also be diluted with a suitable solution before use, and the dilution factor should be smaller. So think more, doing experiments is a combination of mental and physical strength, and I can only tell you that you had to think more before that. ”

Doesn't that sound too inconsistent? Qi Min finally reacted, "Think more" and "I can only tell you to think more before that" are what Zhang Beihai's father said to him in "The Three-Body Problem".

"Wait, you're taking advantage of me?!" Qi Min turned his head to look at Zhang Zhiyong.

Who knew that Zhang Zhiyong said: "You just found out? It's too slow. Look at him—" He glanced back at Ke Li, "and the people are already laughing." ”

It's really boring, Qi Min feels that Zhang Zhiyong's head is not normal, maybe he has read too many science fiction novels. However, she herself felt that there was really no sense of disobedience when she said "think more" at the beginning, and this stalk is actually quite appropriate here.

Of course, the "suitable" here refers to the suitability she uses when she takes advantage of others.

But even if this niche meme is taken advantage of, the experiment still has to be done. Zhang Zhiyong carried out a serial dilution of peptide quantum dots to prevent the quantum dots, which are themselves nanoparticle colloids, from agglomerating. Then Qi Min carefully punched the diluted peptide quantum dots into the pinhole at the rat simulation interface.

After five minutes, Zhang Zhiyong asked Qi Min to rinse the pinhole with normal saline and flush down the unbound peptide quantum dots. This counts as the completion of the fluorescent labeling.

Taking the anesthetized rat out of the safety cabinet, Zhang Zhiyong instructed: "If you want to maintain it for a while, you have to pay attention to avoiding light." Also, remember to play immunosuppressants and stuff like that, I remember that peptides are antigenic. ”

These are all clichés for animal makers, and Qi Min just agreed.

Then Zhang Zhiyong glanced at the watch in the ultra-clean room and said to her: "It's past eleven o'clock now, let's eat?" ”

Qi Min and Ke Li couldn't help but look at each other, in the past, they used to do experiments together and go to dinner together, but Zhang Zhiyong didn't mean to invite Ke Li at all.

I don't know what Ke Li thinks in his heart, but Qi Min knows that Zhang Zhiyong's attitude towards doctoral students is not as kind as her, so he simply agreed: "Okay, let's go." ”