Chapter 271: Days in Princeton (9)

Early the next morning, Junxin, who had come back from the basketball world, had already come to the laboratory early. After a whole month of absence, (half a month to be exact), Junxin still chose to come to the laboratory a little earlier to learn about the current progress of the laboratory.

But in fact, as Junxin envisioned, Princeton is a pocket-sized university known for its research, and no matter what kind of sports or celebration activities it is, as long as it is not related to research, it is basically a niche activity. If it weren't for yesterday's Princeton Tigers' creative win of the university's first NCAA championship since its founding, I am afraid that there would not have been such a big battle. Of course, if it weren't for the fact that the most important person in this game was Junxin, I'm afraid that no one from the school's Academy of Sciences would have come to that dinner.

Combined with his understanding of the University in his past and present lives, Junxin is still very relieved, at least he is very relieved about the laboratory. In Junxin's eyes, the most important thing for a good laboratory leader is to be able to determine a topic and research direction of great research significance, and second, to be able to pull enough scientific research funds. Coincidentally, because of Junxin's special status in Princeton, he can do both. So even if he doesn't go to the lab often, his lab is still the top in the industry.

However, to Junxin's surprise, although he himself had arrived early, there were still people in the laboratory who were earlier than him. Clement-Shashewski had already started his own experiments before Junxin came to the lab.

Junxin changed into a clean sterile operation gown, walked to the laboratory and greeted him softly: "ClΓ©ment, you came early?" ”

"Sir, it's not that I came early, it's that I didn't go back all night!" Glancing back at Junxin, Clement did not stand up, but continued to do his experiment, Junxin could see that he was doing a cell induction experiment.

"Why didn't you go back? I guess the experiment is a bit tight, but it's still in my mind to leave enough time to rest, right? Jun Xin asked puzzled.

"Oh, it's not your fault, sir!" Clement shook his head and whispered, "I don't mean to blame. I volunteered to stay. To be honest, I recently had the idea of replacing my doctoral dissertation while I was conducting your experiments. In the past, my research focused on the induced differentiation of stem cells, but I thought that the reverse differentiation of cell stemness in your project would be very interesting compared to the induced differentiation of stem cells. ”

"As far as I know, if a doctoral student at Princeton University wants to change his research topic, he or she must need the approval of his supervisor, communicate with the university, and then share all the materials he has on the original topic with the person who takes him over. Junxin understands Clement's idea very well, but he still can't believe that Clement has the courage to give up the original results all of a sudden and start over.

"Yes, sir!" Clement gently injected the reagents in the pipette into the T25 cell culture flask, carefully closed the lid, and then said to Junxin seriously, "I think so, sir!" In fact, Princeton also has an unwritten rule, any doctoral student, if his supervisor is in a state of resignation but he does not want to follow the supervisor to leave the school, the university allows him to change the experimental topic and research direction again, clue one after getting your topic, I found after searching for a large number of literature, almost no one in the world is doing relevant experiments and research, you will be the first! In other words, you'll make a name for yourself in the history of stem cell research, and it's a great opportunity for me to rise to a high level of research, so I want to take a gamble! ”

"Have you ever thought about what if I was wrong?" Jun Xin took a deep look at Clement and asked softly.

"Please don't doubt my expertise, sir!" Clement also said seriously, "My professional knowledge gives me the ability to judge and tell me that there is nothing logically wrong with your experiment. In cells, there is still very little research on signaling pathways, but there are still basic understandings. I think there is a signaling pathway that activates the stemness of dry or normal cells through the action of exogenous small molecules, and the experiments I'm doing now are just trying to test this hypothesis. ”

Junxin was noncommittal to Shashewsky's conjecture, but he admired his keen insight and decisiveness, so he didn't say anything, but changed the topic and said, "Where has the experiment progressed?" ”

"Sir, according to the information inquired by Hutt and the information you provided, we are currently screening these maintenance factors, but the workload is a bit too much, so the current progress is stuck in this place!"

"Something to expect!" Junxin had expected this, after all, there was no PCR at this time, and it was very difficult to get a complete maintenance factor, even if Princeton University supported Junxin, it was a very difficult thing to find the relevant records of the maintenance factor in such a short period of time according to the existing literature.

"How many maintenance factors do we have now?" Junxin asked.

"With the information you provided, we have collected a total of 12 maintenance factors from all over the world in a month, which is a full half away from the number of experiments you specified, and these maintenance factors are basically most of the factors in the current cutting-edge research, Hurt seems to have his own ideas about this matter, you can ask him about it at the regular meeting!" Klein replied.

"It seems that a lot of things are not going very well!" Junxin thought with a headache, but after all, he couldn't sigh in front of his experimental assistant at this time, so Junxin was always very calm, and said: "At present, the plasmid construction technology is actually relatively mature, let's use retrovirus methods to carry out the next experiment, first introduce these maintenance factors into the cells, and see if they can work!" ”

"Well, that's what I'm working on."

"In addition, if the remaining part of the virus stock solution, if the single virus is not good, you can consider using a mixed virus, and it is best to find a way to amplify the number of viruses on a certain scale beforehand, and then temporarily freeze it in -80Β°C."

"I'll finish this week."

"Are there any of the 10 maintenance factors I'm talking about?"

"Only 5 of them, and 5 of them are currently operated by Professor Nat himself, and it is estimated that they will not be available until the end of this month at the earliest!"

"Well, mathematically speaking, those 10 may be the most probable, but the existence of antagonism between maintenance factors can be properly considered, so this redundant and large amount of repetitive work needs to be completed with the assistance of all of your laboratory, no problem, right?"

"Yes, there's no problem on my side!"

"For the others, I'll arrange for Professor Nat to coordinate. I think it will turn out to be good. ”

"Sir, I think so too!"