Chapter 049: Super Protein Crystallization

Genius remembers in a second [Aishang Novel Network biquge.info] to provide you with wonderful novels to read.

Fang Jinyan only rested for less than ten minutes, then stood up again and instructed the three girls: "I need your help tonight, I may have to work hard for you." ”

"Whatever you command." Ye Suxue said.

"No problem, what do you want us to do? Did you marinate all of this meat? Liu Yun asked.

"No, I'm going to use them to extract protein crystals." Fang Jinyan said.

"Protein crystallization? What's that? Liu Yun asked in surprise.

"I've told you about evolution and protein-level information before, the root of evolution is to use grade protein to reorganize cells, and there are two main ways to obtain grade protein, the first is self-synthesis in the body, but only the evolutionary body can synthesize, and the degree of synthesis is limited, the second is through guò food ingestion, of course, not all substances contain grade proteins, only the meat of evolution and mutants contains grade proteins. These mutant beast flesh is one of them. ”

"Then why don't you just eat them?" Liu Yun asked curiously.

Fang Jinyan replied: "Directly eating mutant animal meat, the efficiency of absorbing grade proteins is very low, because pepsin will hydrolyze most of the grade proteins into polypeptides, and grade proteins cannot have special functions if they lose their spatial structure, and only a few will be absorbed, which will cause a waste of strict zhòng." Therefore, it must be purified and then injected through the guò method to absorb the grade protein more effectively. ”

"And what do you want us to do for you?" Ye Suxue pulled the topic back and asked.

"It's very simple, the first step in purifying grade protein is to break the cells and release the grade protein and ordinary protein together, what you have to do is to work with me to chop all the more than 500 kilograms of mutant beast meat into meat puree, the more crushed the better." Fang Jinyan said.

In fact, there are better methods of breaking cells, such as mechanical crushing, freeze-thaw and acoustic methods, etc., but Fang Jinyan does not have the corresponding equipment, and even if there is, there is not enough power to drive, so he can only take the most primitive method.

So the four of them started a large-scale meat chopping operation, and it took nearly 4 hours to process all the mutant beast meat, and the three girls all felt unbearable creatine in their arms and were too tired to do it.

But in fact, the mutant beast meat that the three of them handled together was not as good as Fang Jinyan, who held an axe in each hand at a fast rate and chopped it for four hours without a hint of stopping!

The three girls were used to this, and now they understood that Fang Jinyan should be the evolutionary he was talking about, so they didn't treat him as a human being for a long time

Next, the second step of extraction-grade protein is extraction.

Xianfang Jinyan brought a huge glass container, and then used various chemicals to prepare a light yellow solution for most of the container. The process was very complicated, and Fang Jinyan constantly tested the pH value and finally adjusted it to 6.5 very accurately to be satisfied.

These are buffer solvents, which are able to completely dissolve various proteins from minced meat into protein solutions!

With the help of the three girls, Fang Jinyan put all the processed mutant beast meat puree into a glass container containing the buffer solvent, and then held the long axe upside down, using the axe handle to stir it constantly to let it dissolve as quickly as possible.

This process took nearly three hours. The three girls have not been idle, and have been helping Fang Jinyan all the time.

Eventually, the solution gradually changed from the initial light yellow color to an attractive creamy yellow color.

At this time, Fang Jinyan fished out all the meat residues from the solvent and squeezed them dry with special gloves.

Of the more than 500 kilograms of mutant beast meat, there are probably only about 400 kilograms of meat residue left

At the same time, Fang Jinyan obtained about 1.2 cubic meters of protein solution, which, according to his experience, weighed about 1.3 tons, or 13oo kilograms.

Next, there is the third step of extracting the grade protein, rough extraction! That is, the grade protein is extracted from the buffer solvent together with the ordinary protein!

This is a crucial step in extracting grade protein, and there have been countless methods in previous lives, but the results have been very different.

Fang Jinyan adopts the most common and effective one, the organic solvent precipitation method!

Neutral organic solvents such as ethanol, propion(di)ketone, etc., have a lower dielectric constant than water. It can reduce the solubility of most common proteins and grade proteins in aqueous solution, and then precipitate out of the solution, so it can be used to precipitate proteins. In addition, organic solvents can destroy the hydration layer on the surface of proteins, causing protein molecules to become unstable and precipitate.

However, the specific process is much more complicated, such as the dosage and time control of the organic solvent, which are very critical, and the slightest mistake will affect the final extraction effect.

After another hour and a half of hard work, Fang Jinyan finally precipitated about 78 kilograms of colloidal protein from 13oo kilograms of protein solution.

This colloidal protein is still not a true grade protein, in fact its medium protein content is only 1.5%!

Therefore, if you want to get a purer grade protein, you still need to carry out the last step, fine extraction!

Due to the special properties of grade proteins, it is difficult to separate from ordinary proteins, so this step is the most difficult step!

However, in his previous life, Fang Jinyan developed a unique extraction technology, which can be said to be the most advanced technology in the world.

This was one of the important reasons why he became a world-famous chemist in his previous life, and it was also one of the important reasons why he finally lost his life.

At that time, he was young and frivolous, and he had no consciousness that he should keep this secret, and as a result, he was controlled by a big force and lost his personal freedom, although he escaped by luck, but he was still pursued by many forces, and finally he was besieged by a large group of corpses in the process of escaping, and died tragically.

But in this life, this kind of thing will definitely not happen again.

He let the three girls go to rest in advance, and then took the nearly Jiaozhuang protein to another real room, locked the door, and began to extract it alone under the lighting of the homemade power supply

In the early morning of the second day, daylight filtered in through the window, and Fang Jinyan was done.

In front of him was a tray of scales containing 18 crystal clear bright yellow crystals

This is a very regular twelve crystal of exactly the same size! In the light of daylight, it shines beautifully!

There are 18 crystals, each of which is the size of a ping-pong ball. The total mass was measured by the balance to be 1.95 kg.

The average mass is 11o grams each!

That's the grade protein crystallization! It's something that all evolutionaries have dreamed of for a long time!

Of course, in fact, its purity is not 1oo%, but only about 5o%.

Don't think that this purity is very low, in fact, even in the previous life, this is a relatively high purity protein crystal!

As for this life, they must be the purest products to date, if not the first protein crystals in the world.

Fang Jinyan is very confident in this! Otherwise, he wouldn't have been hunted down by so many people in his previous life!