Chapter 59: Penicillin

Back in the courtyard, Ran Yan saw Xing Niang sitting under the porch cutting fabrics, looking dazed, and sighed for a while.

When Xing Niang saw Ran Yan, she hurriedly responded, took the fence, looked at Ran Yan's face, and said distressedly, "Lady is tired, right?" If you return to the main house in a few days, there will be fewer people looking for your illness. ”

"Well, Xing Niang, help me cook the bath soup, I want to bathe." Ran Yan said.

Xing Niang said with a smile, "It's been prepared a long time ago, it's time to take a bath to get sick." ”

Ran Yan's body has only recovered for a long time, and he is already weak, and he has diagnosed two patients and dissected two corpses on this day, which is really a little unbearable.

Soaking in warm water, Ran Yan was a little drowsy.

"Lady, Xing Niang ordered the slave maid to come and wait for you to bathe." A crisp voice sounded outside.

Ran Yan wasn't used to having people watching the bath, but this time she was too sleepy, so she could ask the maid to help wash her damn long hair, so she said, "Come in." ”

The person who came was Xiao Man, who was dressing up for Ran Yan in the morning, she was holding a small bath bucket with a wooden lid on it, and the gap was faintly steaming, and the fragrance of flowers was escaping in the air.

"Yes?" Ran Yan asked.

Xiao Man Ranyan is actually not as scary as it seems on the surface, so he smiled lightly and opened the small dimples on his cheeks, "The slave maid saw that the honeysuckle in the yard was blooming well, so she picked some for the lady to bathe, and it was good to bathe in honeysuckle in summer."

Ran Yan nodded, but thought in his heart, I really guessed correctly for Xiao Song, Fanchun is probably drowned in the bathtub, but ...... Whose tub did she drown in?

Xiao Man saw that Ran Yan didn't have it, so he boldly stepped forward and said, "Lady, can the slave help you wash your hair?" No less words"

"Okay." Ran Yan responded.

Xiao Man pulled a high stool from the corner of the wall, placed it next to the bathtub, and then put the small tub on it.

While pouring water on his body, Ran Yan watched Xiao Man's movements, and his pupils shrank sharply - a high stool barrel

The high stool in the bath room is a little rough, but it is very similar to the stool shape that fell to the ground under the feet on the day when the evening green was hanged, the people of the Tang Dynasty are still sitting, the tables are very low, and there are only Hu beds with legs similar to chairs and stools, and the Hu beds are far from being so high.

Also, this barrel lid......

"Xiaoman, you stand opposite me." Ran Yan said slowly.

Xiao Man was pouring petal bath soup into the bath bucket, and when he heard that Ran Yan remembered her name, he happily responded, and happily put the remaining half bucket of water on the high stool next to the bath bucket, and stood opposite according to his words.

Ran Yan stretched out his hand from the bathtub, touched the small wooden bucket, and poured all the water inside into the tub. Then he began to study the barrel body of this wooden barrel, as well as the barrel lid, from time to time, holding these two things towards Xiao Man's head, Xiao Man's heart trembled for a while, for fear that she would really throw it on her head.

The mouth of this barrel is wide and the bottom is narrow, but it is very heavy, whether it is the bottom or the mouth to hit the human head, it will not just be swollen, at least it must also break the skin, the wound on Fanchun's head is arc-shaped, it should be caused by the lid of this barrel.

The size of the barrel is different, and the arc is also different, and the murderer just took the barrel lid and smashed it on Fanchun's face in a hurry, and then immediately she did not die, nor did she faint......

Ran Yan stood up from the bucket with a bang and reached out to hold Xiao Man's neck.

Xiao Man was stunned and looked at Ran Yan's bumpy body, his mutton fat jade-like skin was shining with water, standing in the smoke, it was simply fascinating.

She was looking at it intently, but her neck was grabbed forward by Ran Yan, and she pressed down the edge of the bath bucket stiffly, her little face almost touched the water, and she hurriedly shouted, "Lady spare your life, Lady spare your life."

Ran Yan came back to his senses, stood naked in the bathtub, coughed awkwardly, sat back in the water silently, and explained to the frightened Xiaoman, "I...... Just thinking of a little thing. ”

Hearing such an explanation, Xiao Manke didn't let go at all, thinking about things, he was holding the lid of the bucket and gesturing her head for a while, and he wanted to press her into the water for a while?

But...... The lady's body is really good-looking, Xiao Manshui's big eyes glanced at the body looming in the water, and whispered, "Lady, the slave maid will wash your hair." ”

Ran Yan nodded, and after figuring out some things at this time, she stopped tossing and let Xiao Man help her comb her black hair that was longer than her waist and hips.

After finally finishing the bath, Ran Yan immediately went back to the room to grind, drew the shape of the bath bucket and the shape of the high stool, and then wrote a letter and handed it to Yang Yong, the team leader who was guarding the Zhuangzi, and asked him to send someone to Liu Tingshi immediately.

After doing all this, Ran Yan really relaxed, and after dinner, she went to accompany Wan Lu for a while, and then went back to the bedroom to rest.

Xu is tired and ruthless, although there are many fragments in this night's dream, but after all, they have never been connected into a paragraph, but it is the best sleep in the past seven years.

In the next few days, it was rainy and rainy, and it was very inconvenient to travel, so the people who asked Ran Yan to diagnose the disease handed letters one after another, saying that they did not dare to bother her to come to the door in person, and would bring the patient to the door on the appointed date.

So these days, Ran Yan is idle, after treating patients every day, he went to Wu Xiuhe to listen to him talk about medicine, since Ran Yan made that meal, Wu Xiuhe is obviously more attentive than before, more hardworking, from time to time openly and secretly signaling that she should cook a meal to reward him for his hard work.

Before I knew it, five days later, the penicillium in the medium was almost ready to extract penicillin.

Ran Yan poured the liquid from thirty petri dishes into a special wooden barrel prepared in advance, then poured vegetable oil into the barrel, mixed and stirred.

Penicillium water contains a fat-soluble substance, and oil can dissolve and separate this fat-soluble substance. Once the mixture is well combined, let it stand.

Eventually, the contents of the barrel will be separated into three layers, the first layer is the fat-soluble substance dissolved with oil, and the second layer is the insoluble substance that is insoluble in water and insoluble in oil, because penicillin is a water-soluble substance, so the water that sinks at the bottom contains penicillin.

The bottom of the special wooden barrel is made to look like a wine barrel, there is a wooden stopper, pull out the wooden stopper, and the water at the bottom slowly flows out, and Ran Yan receives it into a porcelain bowl that has been sterilized with wine and distilled water.

After filtering some of its large impurities, it is poured into a jar filled with boiled and sterilized crushed carbon, and after stirring, the penicillin is absorbed by the carbon.

Xing Niang, who was helping on the side, looked at Ran Yan with a blank face and poured a bunch of bottles and cans, and didn't understand that a bucket of moldy water and seven days of water would be different after oil and carbon?

In fact, these steps of today's operation are not difficult at all, the difficult thing is the preparation of these materials, just the wine and distilled water for disinfection make Ran Yan tired and busy every day, especially the collection of distilled water, although it is not difficult, but the amount is small, it costs a lot to collect a bottle, and when disinfection, most of the bottles are poured out at once, which is simply pouring Ran Yan's blood and sweat

There is also sodium carbonate required for filtration, commonly known as alkali and soda, which must be made from salt, sulfuric acid, coal, and limestone.

After Ran Yan stirred the carbon and water-soluble substances evenly, he purified them three times. Add separately: distilled water, baking soda, weak acid water (vinegar + water).

Add distilled water to filter for the first time, and the poured water is not a penicillin solution and can be discarded.

Add weak acid water the second time, and the filtered water is still thrown away.

The third step is to add baking soda, and the filtered solution is the real penicillin aqueous solution.

Ran Yan looked at the four bottles containing the aqueous solution of penicillin, she had always been calm, her eyes were slightly reddened - it was really not easy These days, she is tired from distilled water, and her back hurts.

Originally, when he thought about seeing the syphilis on Zixu's body, Ran Yan didn't think much about it, because as a modern person psychologically, a modern medical scholar, he subconsciously felt that the penicillin extraction procedure was a relatively simple thing, but he really did it, there was no soda in the Tang Dynasty, there was no distilled water, fine carbon had to be ground slowly, and the medium solution had to be boiled with taro and rice......

Datang will have such a difficult illness Ran Yanxin sighed, if she could change it, she would rather dissect two corpses a day than make penicillin

"Lady, this is fairy water?" Xing Niang asked in a low voice.

Ran Yan paused for a moment, and then remembered that he had said that he had someone to guide him in his dream, so he had to nod.

Ran Yan poured two drops in another petri dish, which contained Staphylococcus aureus, this strain is easier to get, Ran Yan put it in the culture medium to promote its growth a few days ago, and today it is full. With the dropping of penicillin aqueous solution, Staphylococcus aureus gradually dies out, and the place where the drop is quickly vacated.

Validation is useful. Ran Yan breathed a sigh of relief, if it took Niu Erhu's efforts and this last step was verified to be invalid, then she would really cry, fortunately, God was not mean to her.

Ran Yan Yiyi put away these bottles of hard-won penicillin, and finally put down a big stone that was pressed in his heart, and he was quite relaxed, so he decided to cook a meal for the cheap master.

Wu Xiuhe stretched his neck and waited for a long time, Ran Yan had just made the first dish, Ran Yunsheng came, angry old man blowing his beard and glaring, the fairy wind bones that he had worked so hard to maintain, instantly collapsed, sprinkled and tricked, and did everything.

Ran Yan had no choice but to make a dish of his favorite Dongpo meat, one meat and one vegetable, to comfort his broken heart.

Ran Yunsheng smiled like a flower, "Ah Yan, your master is really interesting." ”

The corners of Ran Yan's lips curled slightly, and instead of answering the topic, she asked, "Are you done?" ”

"You forgot?" Ran Yunsheng paused and said angrily, "Today is the Qixi Festival of Qixi Beggars, you can forget everything as a daughter."

"Oh." Ran Yan remembered that Xing Niang had been busy chattering since early in the morning.

"Hurry up, go and freshen up, let's go to Pingjianghe tonight, Uncle will let me take you there too" Ran Yunsheng hurriedly urged.

Yes By].