Chapter 507: Find a way to extract penicillin
The potion in the bottle immediately flowed out of the needle tube along the backflow, and Ye Ruri squinted her eyes and then let the broken flow pinch the tube, and she pulled her snow-white arm out of the bed.
White skin is good for this, and you don't have to work too hard to see where the blood vessels are.
"Queen, what do you want to do?" Breaking the stream and watching Ye Liuli stared at the arm of his son, a little frightened. You don't want to stick a needle into it, do you?
"The water in your family's body is lost too quickly, and if you don't replenish it, you will die of dehydration!" Ye Ruri explained, while taking the needle from the broken hand and carefully piercing it, and soon the blood in the hose backflowed.
After Ye Liuli saw it, he asked Yuli to release the hose, and then saw that glucose water was slowly flowing into the body of the broken stream. In order to fix it, Ye Liuli took a strap to press the needle on the arm, which could not be too tight or too loose, which was really a technical job.
After everything was done, Ye Ruri let out a sigh of relief.
Wiping the sweat from her forehead and glancing at the flowers on the bed, Ye Liuli felt that she still had a lot to do on this trip.
"Keep an eye on your family lineage, when the water in the bottle is about to run out, immediately replace him with another bottle, don't let the air in, otherwise you will die, you know?" Ye Liuli said to the broken stream with a very serious expression.
Broken Stream swallowed his saliva in fright, can such a difficult task not be handed over to him?
"I'm a little tired, let's get some sleep! Let's talk about the situation again......" Ye Liuli felt that her physical strength was very overdrawn, and after saying hello to the broken stream, she went to sleep in the next room.
Yuli automatically stayed behind to help, staring.
He didn't dare to let Rong Shizi appear, although his master was Princess Liuli. But the old prince also told him that he wanted him to take care of Rong Shizi.
Yes, this is the old prince's entrustment, and he has to keep an eye on it.
And from listening to Ye Liuli say that, he stared at the transparent glass bottle above his head without blinking, for fear that the potion in the bottle would be gone in the blink of an eye, and his family would be miserable.
Look at the bottle, not to mention, but also to check the needle on the arm back and forth. In an instant, Broken Stream felt that this feeling was even more difficult than letting him go to the battlefield to kill the enemy, and his eyes were extremely dry.
In fact, after Ye Liuli's timely remedy, Hua Rong's complexion finally improved. Although he is also accompanied by vomiting and diarrhea, the sugar water has been replenished for him and it will not cause a dangerous situation.
After sleeping all night, Yeliuli did not stop, and she was very clear about the epidemic outside. It's no different from cholera on that plane, where everyone calls it a plague but doesn't know it's cholera.
Luckily, she knows a little bit about that. All we need now is to look for penicillin, but where is there any penicillin here? Yeliu was melancholy, although he was asleep in bed, but his mind kept recalling penicillin.
As long as there is penicillin, then this cholera is solved.
How exactly do you extract penicillin?
Ah, she suddenly remembered the Baidu she had seen before, which taught how to extract penicillin using the soil method.
She remembers the above theory, the principle: first collect a large amount of Penicillium, culture it with a nutrient solution, then talk about the culture liquid filtration, add canola oil and stir. After stirring, the water (refined culture medium) is extracted. Through the above method, most of the intolerant substances and lipid substances are removed.
The charcoal is ground into a powder, and the refined culture medium is added to allow the charcoal to absorb penicillin. The carbon that has absorbed penicillin is placed in a container such as a separating column, washed with distilled and acidic water, and then rinsed with alkaline water. Then the demarcated penicillin will be divided into a certain part, concentrated and then dissolved, which is the separation column color layer separation method.
Staphylococci can be cultured with agar medium and tested for drug susceptibility to determine the significant effect.
Realization: First of all, it is necessary to collect a large number of penicillium (steamed buns, steamed buns, fruits, noodles). The rice was ground into juice mixed with the juice boiled with taro as the culture medium, and Penicillium was implanted for 7 days. The culture broth is filtered through a filter cotton, and canola oil is added after filtration to dissolve the lipid-containing substance, and since penicillin is water-soluble, the water under the oil dissolves penicillin.
Add the water to a container with activated charcoal (boiled and sterilized) and stir. These activated carbons are placed into a separating tube (with a large opening at the top and a small opening at the bottom), washed with distilled water, then injected with acidic water (made with vinegar) and finally injected with alkaline water (seaweed boiled juice). At this time, filter cotton is added to the lower outlet to reduce the flow rate. Divide into multiple portions per 100cc.
Staphylococci were obtained from the patient and cultured in medium. Apply the different divisions to staphylococcal Petri dishes. After a few days, if the staphylococci only grows around the penicillin applied, then the division is considered effective.
These are all popular knowledge on Baidu, but she still remembers that there is a TV "Benevolent Doctor" that explains in detail how to extract penicillin and then extract it, and she watched it carefully with great interest and tried it over and over again.
It's just that she did everything out of interest, and she didn't care about the fate of the penicillin she cultivated at that time. But now I can't take care of the temporary cramming, the plague is still spreading in a large area, if we don't speed up and suppress the entire apocalypse, even other countries will be in danger.
It is not an exaggeration to say that the destruction of humanity!
Thinking about Ye Liuli, she felt that things were completely urgent, and she had to speed up her pace to do things.
So when she woke up in the morning, Ye Liuli asked Yu to leave to collect steamed buns and other things with moldy and rotten things for her. Faced with such a strange request when Ye Liuli woke up, Yuli was powerless to complain. But vaguely believing that her request must be related to the epidemic, she was busy going out.
After arranging the top priority, Ye Liuli went to Huarong's room with a somewhat relaxed mood. I just wanted to open my mouth to ask how the master of the Broken Stream family was, but I didn't expect the person on the bed to open his eyes at this time, and his eyes widened in a daze the moment he saw Ye Liuli.
"Heh......" Hua Rong laughed self-deprecatingly: "Are you dreaming again?" After speaking, he slowly closed his eyes. But the discomfort brought by his body made him open his eyes suddenly, this is not a dream!
Thinking about it, he struggled to get up from the bed suddenly, and then saw the figure of Ye Liuli turning around and leaving, and he was about to step forward to stop him: "Liuli, don't go......"
"Shizi ......" didn't expect his own Shizi to react so much, and even fell to the ground with the bottle, the potion in the bottle suddenly flowed to the ground, and the needle on his arm was also broken by his struggle, and the blood flowed down the needle hole, which was shocking......
"Don't go, Liuli......" Hua Rong didn't care about this, and still struggled to stop Ye Liuli's footsteps. Yeye Liuli, who stopped at the door, looked at the sky speechlessly, can this pit daddy not arrange for them to meet so soon? She is completely unprepared to meet this person now, okay?
"Break the stream and help your master to go to bed......" Such a flower, Ye Liuli can't leave. had to turn around and command Broken Stream, Broken Stream nodded and then hugged his family's son to go to bed.