In the dark laboratory, Lake is preparing for the experiment, for this experiment Lake has already lost his money, just to rent this laboratory, Lake spent twenty magic stones. Pen @ fun @ pavilion wWw. ｂｉｑUｇE。 ｉｎｆｏ

If this experiment is not successful, then Lake will have to suspend the experiment and switch to another companion worm.

Putting on a pair of white gloves, Lake pulled out a pale green piece from the glass jar, and the liquid dripped to the ground.

This green lump is the result of Lake's cultivation these days, this thing is a collection of virus parasites, and what Lake needs to do now is to isolate the virus.

The method of isolating the virus can be divided into the following four steps.

In general, the virus can be isolated by passage cells, primary cells, or directly with animal inoculation or chicken embryo inoculation, and only when there is no primary cell or subpassage cell suitable for virus growth, the virus can be isolated by animal inoculation or chicken embryo inoculation.

The general process should look like this:

First, to obtain virus isolation samples, this requirement is relatively high, if it cannot be operated immediately after collection, it must be stored at low temperature and sent to the laboratory for operation as soon as possible. In addition, it can also be cryopreserved, and the virus is not easy to freeze to death under normal circumstances. Of course, there are some special viruses, which require reference to the relevant information.

In the second virus isolation operation, the disease sample will be obtained and grinded, and frozen and thawed at least once, of course, the grinding process must be at a low temperature. At the same time, the grinding should be sufficient, normal saline or PBS can be added or directly added to the cell culture medium, after the grinding is complete, freeze-thaw one to three times, the purpose of freeze-thaw is to allow the cells to completely rupture and release the virus in the cell into the solution. Then centrifuge at low speed and take the supernatant with 0. Filter through a 22 micron membrane to remove the filtered liquid after seeding the cells. At this time, it should be noted that not all viruses need to be completely filled with cells, generally viruses that cannot produce cell diseases are best inoculated when the cells have grown to about 40%, and those that can produce cytopathies need to be completely filled with the cell bottle before inoculating the virus. Bispecific antibodies or other antibiotics can be added to avoid contamination during inoculation.

Thirdly, after inoculation, some viruses that can produce cytopathies are easy to observe, and you can see whether the isolation is successful directly under the microscope, if the virus does not produce cytopathies, it needs to be detected by other methods, such as fluorescent antibody staining, PCR or other methods. However, in most cases, the first generation is not easy to see or the effect is not good, so you can pass it on to a few more generations. If there is no more transmission after four ~ five generations, then congratulations, you have failed, or there is a problem with your method, or there is no virus you want to isolate at all, or your virus has died in the isolation process.

Isolation of viruses with cells is probably this process, of course, some viruses may not have the corresponding passage or primary cells, if you want to isolate you can only use susceptible animals, the operation is similar, nothing more than sampling, sample processing, inoculation, post-inoculation detection and observation, and virus collection. Of course, if it is not a tissue, for example, if you want to separate it from feces or urine or swabs, you can directly dilute it with normal saline, PBS or cell culture medium (to shake as much as possible, if it is a swab, you should squeeze and shake well), and then centrifuge the supernatant and filter the inoculated cells.

Lake cautiously began to isolate the virus according to the requirements, but it was not easy for Lake to isolate due to the relatively backward equipment in this world, but after several efforts, Lake still sorted out some viruses.

These viruses take on a faint black, somewhat strange color in the test tube, and these viruses have been modified by Ryker's cultivation and witchcraft.

While these viruses still have the ability to differentiate, they also have controllability, which is simply to make this virus change from harmful to companion.

In this way, the virus is parasitic in the host, and the host can use the virus's abilities, but the virus cannot pose a threat to the host.

The experiment was a success, but of course it took a long time, from the beginning of the research to the end of the study.

You must know that this is still because Lake has the chip, otherwise the complex operation is enough to make anyone feel desperate.

The large amount of genuine and expensive materials consumed in it is not enough for outsiders, but it is definitely enough wealth to make any second-level wizard apprentice desperate.

And all this is because of this potion in Lake's hand, which is the virus that Lakeke cultivated, which is the best companion worm in Lake's mind.

The virus solution in the test tube was poured into a specific container, and Lake began to chant a strange incantation.

As the sound of the spell echoed through the room, the virus inside the container made an inaudible sound.

This frightened Lake a little, Lake had always thought that these things were a virus, but now it seems that Lake was clearly wrong, this is not a virus at all, but a very small, very small bug.

Although he was a little surprised by such a small bug, it was more of a surprise to Lake, after all, the requirement for the companion insect was originally a bug, and he was not very sure about the virus Raike before, but now these things turned out to be bugs, and Lake's grasp suddenly became a lot more.

The spell was still moving, but there was already a delicate bead of sweat on Ryker's forehead, and these companion insects needed to "purify" the soul.

Although Lake's history is relatively strong, there are too many of these bugs, so it is also quite difficult to purify.

"Quack, quack, finally purified. Lake placed the decontaminated companion worm in a syringe and injected it into his vein.

As soon as the companion worm enters Ryker's body, it begins to multiply wildly, and then parasitizes Ryker's cells to the fullest.

Then Ryker's body also changed, and the flesh and blood of Ryker's whole body tended to be differentiated, and the tendons, muscles, blood vessels and organs in his body seemed to have become independent individuals at this moment, not under Ryker's control.

"I created you, and you will surrender to me. Lake let out a laugh like a night owl.

The powerful spiritual power directly chose the most brutal suppression, but Lake obviously underestimated the abilities of these companions, or overestimated his own abilities.

ps: Regarding the question of magic pets, most of them are more in favor of crows and bats, and some talk about cats and three-headed dogs. But the rabbit that eats meat, is this okay? The author thought about it, and it seems that he can really love it, or the rabbit ???

ps: Ask for a collection and a reward, all kinds of !!!!!!