Under Jiao Hong's arrangement, soon, Lin Yu wore a white mask and white overalls and walked into the GMP laboratory with her.

Ouyang Lei and Zhou Yang, two non-professionals, stayed outside and did not come in.

After entering, Lin Yu found that there was already a team of twenty gene editing teams waiting for him.

Seeing this, Lin Yu didn't talk nonsense, and said straight to the point: "I won't say anything superfluous, I guess you know very well the purpose of my coming today, so please listen to my command next." ”

"From now on, you form your own team, a group of five, a group of people, and you use a blood cell separator to apheresis about 50ml of mononuclear cell suspension in the venous blood that has just been delivered."

"People in the second group, you enrich T cells, and the enrichment can be done by density gradient centrifugation."

"T cell subsets are isolated by CD4/CD8-specific antibodies or surface marker-conjugated magnetic beads, and the cells are stimulated by CD3 and CD28 antibodies to activate and isolate them."

"Three groups, you prepare lentiviral vectors, cells, culture media and other auxiliary tools, and make sure that all water sources in the experiment are ultrapure water."

"Four groups, you are responsible for filling the vacancies, and you will go wherever you need them."

As one command after another came out of Lin Yu's mouth, the originally somewhat dazed researcher also began to operate.

Under the leadership of Lin Yu, the whole team is running efficiently like a starting machine.

Seeing Lin Yu commanding each group of people so skillfully, even Jiao Hong, who was visiting on the side, was very surprised.

Is this the chairman or the head of a large team of professionals?

This professional knowledge and the proficiency of the conductor are too exaggerated.

If she hadn't seen it with her own eyes, she really had a hard time believing that this was actually the chairman of their company.

Seeing that the entire laboratory had begun to operate, Lin Yu also nodded with satisfaction.

The whole CAR-T cell technology is divided into three parts.

The first step is to isolate the T cells and activate them.

The second step is to genetically edit these isolated T cells and upgrade them so that they can find and kill cancer cells with precise guidance.

The third step is to grow a large number of upgraded T cells and inject them into the body of the cancer patient who had the blood drawn.

Finally, it is only necessary to observe the adverse reactions slowly.

Although it is simple to say, the second step in the whole step is the most critical and important.

Especially gene editing, the core technology involved in it, except for Lin Yuneng, no one else can develop it at all.

As if thinking of something important, Lin Yu hurriedly looked at Jiao Hong and asked, "Can the ultrapure water in our laboratory meet the data requirements of the Pharmacopoeia in terms of sterility and endotoxin?" ”

Hearing this, Jiao Hong said without hesitation after coming back to her senses: "At present, the high-quality ultrapure water used in our laboratory not only meets the data requirements, but also exceeds. ”

"Well, that's good."

Lin Yu nodded and continued to pay attention to the entire experimental process.

Three hours later, the tasks of the first and second groups were completed one after another, and there were no mistakes.

Seeing this, Lin Yu also breathed a sigh of relief, because the next step is the preparation of the most important CAR-T cells.

This step is the most critical, and it is also the most core technology.

Lin Yu came to the experimental table, and with the assistance of the third and fourth groups, he picked up the tools in his hand and devoted himself to the preparation of CAR-T cells.

CAR-T cells are prepared by genetic engineering technology to add a chimeric receptor to T cells that can recognize tumor cells, activate T cells at the same time, and ultimately kill tumor cells.

Related genetic engineering technologies include lentivirus, γ retrovirus, Sleeping Beauty transposon system or mRNA transfection, mRNA electroporation, etc.

And Lin Yu uses lentivirus.

Through it, Lin Yu can effectively integrate foreign genes or exogenous shRNAs into the host chromosome, so as to achieve the effect of persistent expression of the target sequence.

In fact, to put it bluntly, it is to use gene technology to transmit the sequence that can produce CAR into T cells.

As Lin Yu carried out gene editing, all the researchers' eyes were focused on Lin Yu, who looked serious, and they were very fascinated one by one.

In this state of extreme concentration, time simply did not pass too quickly, and the sky had already darkened completely.

At the same time, outside the laboratory.

Ouyang Lei and Zhou Yang, the two bosses, actually leaned against the wall with a bowl of spicy instant noodles.

Soon, the fragrance gradually became stronger, and the two began to sip instant noodles while looking into the laboratory from time to time, talking to each other.

"Whew, whew, hiss...... Mr. Lei, how long do you think it will take for Lin Dong to complete? ”

Zhou Yang blew the hot instant noodles a few times, and then couldn't wait to stuff them into his mouth, and said vaguely.

Hearing this question, Ouyang Lei, who was finishing the last bite of instant noodles, couldn't help but look at the time on his watch.

At this time, the watch showed that it was already about 12 in the morning.

"It should be soon, I remember the time when Lin Dong finished developing BTK inhibitors yesterday, it seems to be at this time."

Putting the finished instant noodles under the trash can on the side, Ouyang Lei hesitated for a moment, and said with some uncertainty: "However, this CAR-T cell technology may be a little more difficult, and it is estimated that it will take a few hours." ”

From the afternoon to now, a full half day has passed.

The two of them didn't do anything outside, so they were a little hungry and couldn't stand the news, so they could only eat instant noodles to satisfy their hunger.

And now, Lin Dong and the researchers in the laboratory are still focusing on the research and development of CAR-T cell technology without eating for half a day.

This made the two feel that these people were not easy, and at the same time, they were also a little embarrassed.

Especially their chairman, as a boss-level figure, Lin Yu is still hungry and personally fighting on the front line.

This comparison also made the two feel a little embarrassed.

But people are iron, rice is steel, and when they are hungry, they can't do anything.

Soon, the clock continued to pass and it was one o'clock in the morning.

In the laboratory, Lin Yu cloned the lentiviral vector through his own synthetic CAR element to package the pseudovirus.

These pseudoviruses are then used to infect T cells isolated from the patient, and then CAR-T cells are gradually produced.

After completing this last step, Lin Yu looked at the CAR-T cells in the petri dish and couldn't help but raise a bright smile on his face.

After seeing Lin Yu stop what he was doing, all the researchers present came back to their senses one by one as if they had just woken up from a dream.

"Good news for you."

Putting the tools in his hand on the experimental table, Lin Yu turned around, looked at the researchers around him with a smile, and said slowly: "About CAR-T cell technology...... We did it!! ”

Lin Yu's voice went from low to high, from a sudden pause to a final outburst.

The tone was suddenly frustrated, and it immediately detonated the emotions of all the researchers present!

Rumble!

After hearing the news, all the researchers in the entire lab only felt that their brains went blank.

After a brief silence, excited cheers erupted, and the entire laboratory seemed to be overturned.

"It worked! We did it!! ”

"Hahaha, it's like a dream!"

"Unexpectedly...... It's really a success..."

"Woo woo, the CAR-T cell technology turned out to be successful, they were saved, they were finally saved."

The excitement and joy on the faces of some young male researchers in the face of this exciting news were palpable.

They hugged each other, cheered and jumped for joy, and were very excited.

Some sensual female researchers thought of something sad and cried bitterly on the spot.

Even those researchers who looked like steady middle-aged people were also muttering to themselves in place, which was very shocking.

Because this impossible thing happened to them, it really made them feel like they were daydreaming.

CAR-T cell technology, a problem that plagues all medical companies around the world, has been developed by them......

No, they didn't develop it.

It's their chairman who developed it!

This is well known to all the researchers present.

They're just playing on the sidelines.

In addition, they have been observing their chairman's gene editing technology almost the whole time, which is basically a thorough assistance.

This kind of thing can be done by a professional team.

But even so, their chairman is willing to share the research results with them.

This kind of supreme honor, which is enough to make the world's top gene editing team envious, was easily obtained by these people......

At the thought of this, all the researchers felt incredulous and at the same time, they were full of gratitude to their own chairman.

And outside the laboratory.

Ouyang Lei and Zhou Yang, who had been paying attention to the situation inside, were also excited when they saw all the researchers inside cheering.

"Beautiful! It was really developed! ”

Thinking of the impact of CAR-T cell technology, even if Ouyang Lei was prepared, he was still blushing with excitement and waved his right hand that was clenched into a fist fiercely.

"Even the top genetic team in the United States has not solved the problem, but it was solved by our chairman......"

Zhou Yang also sighed with a look of amazement.

If he hadn't seen it with his own eyes, it would have been hard for him to believe that such a thing was happening.

It's unbelievable, and it's even a miracle.

Moreover, what Zhou Yang didn't expect the most was that this miracle was actually created by the newly appointed chairman of their Tianjing Company today...

Inside the laboratory.

After a short period of excitement, everyone also began to quiet down, and one by one they cast their fiery eyes on Lin Yu in the center.

Seeing this, Lin Yu also smiled slightly, picked up the petri dish on the experimental table, and said, "Everyone, please see, the cells in the petri dish in my hand now are the CAR-T cells that I have embedded with receptors. ”

"At present, their number is still too small, and it is difficult to kill a large number of cancer cells in patients, so the next step is to culture CAR-T cells in vitro to make their number reach the therapeutic level, such as hundreds of millions."

"At that time, only the cultured CAR-T cells need to be infused back into the patient, and they will function in the patient's body."

"For example, once cancer cells are found, CAR-T cells will not only kill them, but also begin to multiply themselves, creating a huge army of cancer killers to destroy cancer cells together."

"Of course, the CAR-T cells in my hand can only be used to treat the patient who draws venous blood, and other cancer patients need to re-prepare the CAR-T cell ......."

After briefly introducing some details, Lin Yu handed the petri dish in his hand to Jiao Hong on the side, and said with a smile: "I've finished saying everything I should say, and I'll leave it to you next." ”

"Well, don't worry, Lin Dong, I will take care of everything seriously." Jiao Hong carefully took the petri dish with both hands and said with great certainty.

Hearing this, Lin Yu just smiled and nodded.

Suddenly, as if he remembered something, he hurriedly added: "By the way, before the patient is infused back with CAR-T cells, if chemotherapy is carried out some time in advance to kill a large number of cancer cells in the body, the treatment effect will be maximized." ”

"Okay, I got it."

Jiao Hong held the petri dish that attracted the attention of all the researchers in both hands, and said in a somewhat impatient tone: "If there are no other problems, then I will take someone to culture CAR-T cells in vitro." ”

Looking at her impatient appearance, Lin Yu also waved his hand with a smile and motioned for her to go.

Seeing this, Jiao Hong held the petri dish in both hands and took all the researchers present to transfer their positions to the special cell culture area.

Soon, the group directly devoted themselves to the in vitro culture of CAR-T cells.