Bai Shulong said that he would let Yang Zhou give it a try, but in his heart, he wanted to wait for Yang Zhou to encounter a setback, so he persuaded him to change the subject, anyway, he said that he would give Yang Zhou half a year.

If you pour cold water on it now, you will be slapping yourself in the face.

Next, Bai Shulong really cooperated with Yang Zhou, and took the initiative to ask what equipment was needed and how much money was needed.

He's here to help with the coordination.

Yang Zhou calculated the expenses, and the biggest expense was protease.

That is, the Cas9 protein (Cas9 nuclease) used for gene editing.

RISPR/Cas9 is an adaptive immune defense system formed by bacteria and archaea during long-term evolution.

The CRISPR/Cas9 system provides immunity by integrating fragments of invasive phage and plasmid DNA into CRISPR sequences and utilizing the corresponding CRISPR RNAs (crRNAs) to direct the degradation of homologous sequences of the Cas9 protein.

The artificially modified Cas9/sgRNA system uses sgRNA (short guide RNA) to guide the Cas9 protein to recognize and cleave double-stranded DNA with sgRNA targets, which can be used for gene knockout and precise DNA editing.

Both animals and plants have a DNA structure, so Cas9 technology can be used.

If you think of DNA as an old-fashioned roll of film, and there is a piece of film that you want to cut off, you can use Cas9 protease to replace this piece of film, and the original film will fail, and the DNA chain will be broken.

After a DNA strand break, CRISPR/Cas9 has two options: either directly add bases to destroy the original gene function and achieve gene knockout by silencing the gene, or use a single-stranded DNA template to introduce a new sequence and edit the gene.

The same is DNA film, and GMO is equivalent to using another roll of film and sticking it to the original film, so that the original film can show a different picture.

Genetically modified soybeans are to add petunia resistance genes to the DNA fragments of soybeans, because petunia resistance genes have the ability to resist the herbicide glyphosate, which can make genetically modified soybeans not killed by herbicides, and all weeds will be killed by herbicides.

That's the difference between the two.

Strictly speaking, transgenic is also a type of gene editing, Cas9 gene editing is subtraction, and transgene is addition.

What Yang Zhou is currently preparing to use is subtraction.

Subtraction is not so easy to achieve, because the protease for gene clipping also needs to be designed, the most critical of which is to obtain double-stranded DNA with sgRNA targets.

Cas9 is an editor, for example, to edit the genes of turnips, so you always have to know which part to clip.

Nowadays, many laboratories have the business of designing Cas9, and the cost of the editor is also different, some have a low success rate and the price is cheap, and some have a high success rate and the price is relatively expensive.

According to the calculation of 10 micrograms, the price is between 1,500 yuan and 15,000 yuan, and the price varies depending on the quantity.

Yang Zhou chose to order from Thermo Fisher Scientific, a global supplier of experimental instruments and equipment, with a variety of experimental reagents and equipment to supply.

All businesses can be customized online.

You can also add customer service specialists to customize the experimental instruments or materials you want.

Of course, this price is more expensive.

Yang Zhou ordered 500 micrograms of Cas9 protein at a price of 13,935 yuan.

Plus the freight, about 14,000 yuan, it is expected that the materials can be obtained within 1 week.

With Cas9 protein, Yang Zhou can make the part of the gene that needs to be knocked out of the carrot through the experimental instrument, transcribe it into the protein, and then inject it into the carrot cell, and finally the corresponding gene can be knocked out.

This project belongs to Yang Zhou's master's and doctor's thesis project, and it is natural to use the university's scientific research funds.

For example, if this achievement can open a company to make money, then it needs to be united to do business, such as Qingbei University gave birth to Founder Group, and HKUST also produced iFLYTEK Technology.

Yang Zhou doesn't mind the carrot project being run by the school, it's just a single research achievement.

The technology that can be used on carrots cannot be used on other plants, and each plant needs to be designed separately.

The carrot project is Yang Zhou's stepping stone in scientific research.

Yang Zhou applied for 20,000 yuan for the experimental fund, and the experimental equipment school has it, so there is no need to buy it separately, and the money for carrot seeds is not to be mentioned.

Bai Shulong is very powerful, since he decided to support his students to do experiments, the funds were approved on the same day.

20,000 yuan, still deducted from his own funds.

Although Yang Zhou is also a small net worth, he never thought about paying for the carrot project, he still hopes to deepen cooperation with Qingbei University, and is willing to hang the name of Qingbei University if there are results.

After hanging the name of Qingbei University, it is easier to publish.

After the publication of this paper, Yang Zhou should be able to graduate, and then he can choose a postdoctoral fellow, or even a temporary position, so that he can use the name of Oasis Ecological Biotechnology Company to cooperate with Yang Zhou to establish a laboratory in the school.

The school will also encourage external funds to invest in the school, because although the final benefit of the results researched in the school laboratory belongs to the company that provides the funds, the corresponding author of all the results is the school.

The more papers published, the more top-notch the papers, the more representative the strength of the school.

The stronger the university, the more attractive it will be to scientific research scholars and students, and the more scientific research funds will be approved by the state, which is beneficial to all parties.

This is almost the integration of production, education and research as the news media said.

Yang Zhou began to do his own doctoral graduation project, Tang Yue also cheekily said that he wanted to join, Liu Tao and Xu Fu are both undergraduate students who graduated with a master's degree and a doctorate, so they naturally know the difficulty of Yang Zhou's topic, and they don't want to get involved at all.

In their opinion, Yang Zhou estimated that if he persisted for 1 month, he would end in failure, although he would not see the jokes of his senior brother, but he would not take the initiative to help.

Learning things from Professor Bai's side is much better than following Yang Zhou's blind tinkering.

In the end, the laboratory was divided into two waves, Professor Bai took two master's and doctoral students to continue to study the molecular mechanism of hybridization, and Yang Zhou took his little sister to do the topic of carrot gene shearing.

The two research groups share the planting and cultivation room.

Of course, Yang Zhou hasn't researched anything now, and he doesn't need the cultivation room yet.

While waiting for the Cas9 protein to arrive, Tang Yue kept circling Yang Zhou, asking what to do now, but Yang Zhou was always closing his eyes and recuperating.

was annoyed by being entangled, so he sent Tang Yue to keep an eye on the progress of Lu Siyao and Wang Binxiang's charity activities.

Anyway, Tang Yue has another identity, that is, Yang Zhou's little secretary.

As for the money-making vegetable wholesale project, Yang Zhou is now fully entrusted to the invited Zhong Hong, who is responsible for sending more than 100 tons of goods every day, and he can receive the second payment in a few days.

Yang Zhou is not doing nothing these days, but is thinking about the gene editing function of the system, and is studying carrot genes in detail.

After many days of research, Yang Zhou found that the most anti-heaven function of the plant black technology system is not those few pieces of land and space spring water or seed mall.

The most rebellious thing is the gene editor!

When Yang Zhou put the carrot into the gene editor, all the 32,113 genes of the carrot appeared in Yang Zhou's mind in the form of virtual images.

The editor didn't tell Yang Zhou what each gene was doing, but Yang Zhou was able to mindfully eliminate any of the 32,113 genes.

It's like an extra biology lab in the brain!

At the same time, there is no need to plant, and the plants after gene removal will quickly grow into virtual entities, so that Yang Zhou knows the final result.

This is Yang Zhou's greatest confidence!

Others study gene clips and want to know the impact of genetic changes in carrots, and they have to wait at least 105 days for the radishes to mature, and Yang Zhou immediately knows the answer.

In the world, there is no laboratory that can catch up with the progress of Yang Zhou's experiments.

PS: I'm starting to do scientific research! Ask for support, the progress is not slow, the foundation is relatively solid, there are many follow-up studies, and the protagonist will also establish a national laboratory in Qingbei University!