Chapter 186: Dream Gene Self-Test
In the study, Wu Zhe had already written several pieces of paper. After thinking about it for a period of time, Wu Zhe also had a relatively complete idea of the new genetic mapping method. It's just that now it's feasible to just write a research report to demonstrate the method you proposed.
This time, he completely abandoned the traditional Sanger double deoxy chain termination method. He used what he called a dream gene self-test. The Chinese name is called: Dream Gene Self-Test Method.
Here's how it works: four bases are benchmarked against four different stains. By single-primer PCR sequencing reaction, the resulting PCR product is a single-stranded DNA mixture of four different fluorochromes at the 3" end of 1 base, so that the sequencing PCR products of the four fluorochromes can be electrophoresed in a single capillary. Due to the different size of the molecule, the mobility in capillary electrophoresis is also different, when it passes through the capillary reading window, the CCD (charge-coupled device) camera detector in the laser detector window can detect the fluorescent molecules one by one, the excited fluorescence is divided by the grating to distinguish the fluorescence of different colors representing different base information, and the imaging is synchronized on the CCD camera, and the analysis software can automatically convert the different fluorescence into DNA sequences. So as to achieve the purpose of DNA sequencing. The results can be output in a variety of formats, such as gel electrophoresis patterns, fluorescence absorption peaks, or base sequences.
The principle may be very simple, but to make it, it requires multidisciplinary cooperation. It involves biology, chemistry, computer, physical optics and other disciplines. It can be said that it is a comprehensive application of mathematics, physics, chemistry and biological computers.
Wu Zhe only used mathematical formulas to calculate what requirements each discipline needed. Whether it can be done or not is still up to experts in various disciplines to answer. In terms of Wu Zhe's current strength in various subjects, he is not enough. He is more powerful than the average person, but not to the extent that he is an expert in every subject.
If the conditions of other disciplines are not enough to meet his requirements, then the topic can only be published as a paper.
When he was done, he read it again. Again, I calculated one side in my mind. Anyway, according to his mathematical logic, as long as the conditions keep up, then this dream mapping method is completely feasible.
I stretched and looked at the time. It would be the morning of the 3rd. I wrote in my study for a day and a night.
I got up and walked out of the study and into the hall. I saw Tang Meng half-lying on the sofa and falling asleep.
Wu Zhe frowned, knowing that Tang Meng didn't go back to the room because he was waiting for him. stepped forward and hugged Tang Meng.
Tang Meng was already sleeping very lightly, and when he was hugged by Wu Zhe, he was immediately awakened. When he opened his eyes and saw that he was being hugged by Wu Zhe, he asked shyly, "Ah Zhe, have you finished writing the topic?" Where are you going to take me? ”
Wu Zhe smiled and said, "Carry you back to your room to sleep." You're stupid, too, and you can't go back to your room to sleep! What are you waiting for me! ”
Tang Meng grinned and said, "I'm hungry, can I eat something and sleep again?" ”
Carry you back to your room first, you lie on the bed, and I'll bring you it up when I'm done.
Tang Meng didn't say anything anymore and closed his eyes. His hands wrapped around Wu Zhe's neck.
After Wu Zhe put Tang Meng on the bed, he said, "Wait, don't fall asleep." I'll be fine soon. ”
"Then go and show me your report. That way you won't get sleepy. ”
Wu Zhe went downstairs to take his project report to Tang Meng first, and then went into the kitchen, heated the milk, got two sandwiches into the oven, and fried a few eggs.
In a few minutes, Wu Zhe made breakfast. When I came to Tang Meng's room, Tang Meng's report hadn't been read yet. Wu Zhe used to take out the report in her hand: "Eat first, the report will be read when you wake up, and you won't run." “
Tang Meng didn't say more, she would indeed be sleepy and hungry. After eating a sandwich and drinking a glass of milk, Tang Meng felt that his dizzy head was much better. I began to think about the content of Wu Zhe's topic. It's just that I haven't finished reading it all, and it's going to be a little itchy.
Hurriedly ate another egg. Then stretch out your hand. The meaning is obvious, I'm done eating, you report it to me.
Wu Zhe smiled, there was nothing he could do with her. Hand her the report and continue to eat her share of breakfast.
"Ah Zhe, I like the name of this surveying method!" Tang Meng saw that Wu Zhe had obtained the surveying and mapping method, and his name was Dream Gene Self-Test Method. Naturally, I was also very happy.
Wu Zhe smiled and asked, "How do you feel?" Can it be successful? He didn't put gold on his face, this name was important in Tang Meng's opinion. If it succeeds, the dream gene self-test method will naturally be able to spread in the biological field. But if it doesn't work, it's just my own self-esteem.
"There are too many disciplines involved, and many of the things you mentioned in it are already some of my knowledge blind spots, and I can't give you an accurate judgment. But the mathematical model you gave is very interesting, and I can understand what you mean. If you can follow your mathematical model, I think the success rate is very high. Tang Meng also replied with a serious face.
Seeing that Wu Zhe didn't speak, Tang Meng continued: "You can treat this article as a paper, no one has written a project report like you, almost all the problems have been raised, and some solutions have been given. If you're going to submit this paper, I think both Nature and Science can be published, even The Lancet. ”
Wu Zhe said "um", and then said: "Okay, don't worry about me yet, you can sleep first, and we will visit your teacher in the afternoon." ”
Seeing what Tang Meng was going to say, Wu Zhe directly scratched Tang Meng's small nose: "Be obedient, I also want to rest." ”
At this time, Tang Meng flashed his eyes and stopped talking. When Tang Meng fell asleep, Wu Zhe withdrew from Tang Meng's room.
Wu Zhe's energy is not comparable to ordinary people, and after a short sleep, the whole person's spirit is almost back. After taking a hot shower, it's not much different from usual.
I made a cup of tea and took a book casually. Wu Zhe looked at it leisurely, he was very nervous about this day and night, Wu Zhe still likes that kind of slow-paced life, maybe the habits of his previous life can't be changed for a while. Anyway, if people look at it, this rhythm doesn't say that he is an old man! But it doesn't quite match his current age of 15.
I made a lunch at noon, and after eating, I put the dishes in the incubator. When Tang Meng gets up, he will definitely cry hungry.
Tang Meng didn't wake up until almost two o'clock in the afternoon, rubbing his sleepy eyes and looking for Wu Zhe. I saw Wu Zhe reading a book below. said to Wu Zhe: "Ah Zhe, I'm hungry again." Did you make lunch? Grab a bite to eat, and we'll go to the teacher's. ”
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