Seeing that Zhou Qingfang was finally gone, Zhang Chen breathed a sigh of relief in his heart.

He had to admire Zhou Qingfang.

There aren't many people who know what they want, and they act on it with a purpose.

Zhou Qingfang is the king of this kind of people, he can not face in order to achieve his goals.

Zhang Chen was amazed, it was really a big forest, and there were all kinds of birds.

He is not that kind of person, even if there is no system, he cannot be so shameless.

The "Lanyue 001" project will start research and development experiments today.

Zhang Chen took the information that had been prepared a long time ago and went to Tian Wenbin for final confirmation.

"Zhang Chen, are you sure you only choose this one adeno-associated virus subtype now? I compared, this capsid has also been modified to a certain extent, don't you need to verify it carefully? ”

"Tian Bo, don't worry, don't worry about the selection of capsid and therapeutic genes, what we need to do now is to produce a batch of adeno-associated virus products in small batches first, and go to the mouse model to see the gene expression."

Tian Wenbin pondered for a moment, and he didn't say anything again.

He had no reason not to believe Zhang Chen.

Although he had close contact with and even operated the sequencer, he still didn't understand how Zhang Chen got such high-quality sequencing technology.

He has such an unnatural sequencing technology, and it seems reasonable to come up with a less conspicuous gene therapy drug.

Tian Wenbin nodded and said: "Then let's do it first, I have designed several schemes, let Guang Sihui construct the plasmid first, since the yield of this combination is high, then let's choose the insect cell expression system, which can minimize the cost." ”

Zhang Chen deeply agrees that the selection of insect cell expression system is the best way, and there is no need to choose a three-plasmid system.

The main advantage of the three-plasmid system is that the virus yield is higher at the end of production.

However, the combination of viral vector and therapeutic gene given by the system does not have to worry about the yield problem at all.

Therefore, the Sf9 insect cell worship system, which is easier to produce on a large scale, is indeed the best choice for them.

Tian Wenbin is worthy of more than ten years of experience in adeno-associated viruses, and he hit the nail on the head to the core problem.

"Well, we don't need to save, we can buy what should be synthesized, and we can directly use the fastest method to construct plasmids to save time for later experiments."

Zhang Chen groaned and said, "How about Guang Sihui's experience in building plasmids, if she can't do it, I can do it." ”

Plasmid construction is Zhang Chen's old business, he is delicate, and the success rate of construction is extremely high, and Tian Wenbin is full of praise for him.

Tian Wenbin smiled and said: "If you directly synthesize gene fragments, there is not much difficulty in such construction, and you don't need to start it yourself." ”

Between talking and laughing, the two decided on the research and development plan of an epoch-making product.

The system solves the hardest part of early development because they don't have to spend a lot of time designing and optimizing viral vectors or predicting and validating targets.

They only need to produce enough finished virus for mouse experiments on a small scale according to the sequence given by the system.

"Fortunately, I bought an ultracentrifuge and a shaker before, and when the results of the mouse experiment are almost out, the pilot GMP workshop will be almost completed, and then we will go directly to the 200-liter fermenter, and we will strive to submit the CDE in the shortest possible time and apply for clinical trials!"

Zhang Chen was in a good mood.

Although the company can't do mouse experiments yet, there are many CRO companies outside.

As long as the money is in place, these companies, which are mainly engaged in scientific research services, can complete all the experiments of their customers on time.

"It does save a lot of time, provided that the sequence you give is really as powerful as the information says."

Tian Wenbin continued with a smile on his face: "It's just that the data does not mention the specific plan for process scale-up, and this aspect may still need to be explored at that time, but this is after the success of the first phase of clinical trials." ”

Zhang Chen smiled knowingly, he was not worried about the process enlargement at all.

You only need to spend some points to simulate, and then the optimal process scale-up plan will be at your fingertips.

"Don't worry about this at all, I'll be able to give you a solution then."

Zhang Chen continued: "Tian Bo, do you still remember Mr. Jiang, I want to recruit him to the company, you are more familiar with him, why don't you try to contact him?" I remember he wanted to make a difference in cardiovascular disease, and it just so happened that our company was doing that. ”

In his eyes, Tian Wenbin and Jiang Lu are like-minded people, and Zhang Chen wants them to make this medicine with him.

Jiang Lu was a clinical doctor before, and he was very professional in toxicology and pharmacology, and it just so happened that Chenyue was lacking such a person now.

Tian Wenbin was stunned for a moment, he didn't know why Zhang Chen suddenly had this idea, but now that he recruited Jiang Lu over, he did make up for Chenyue's shortcomings.

"Yes, I happened to have been in contact with him recently, I'll ask and see."

After finalizing the basic protocol, Tian Wenbin gave the plasmid construction plan to Guang Sihui, so that she could prepare and immediately carry out relevant experiments.

Today, Zhang Yue recruited two more people, and they will be responsible for R&D experiments in the future, and their main job is to cooperate with Guang Sihui to produce small batches of viruses.

Zhang Chen asked Zhang Yue to find a way to hurry up and recruit more people.

Small-scale production is expected to start in half a month, and now it is necessary to recruit people with production experience in advance.

He wanted to get the data within a month and submit it to the CDE for clinical trials.

If you delay the whole progress because you didn't recruit people to do production, it will become a complete joke.

However, in this regard, he is still more assured that Zhang Yue, as long as the money is in place, she can let a large number of headhunting companies poach people at the same time.

Zhang Chen believes that with the salary level he gave, there will be a large number of people who will come to the chrysalis.

........

Qian Youwei was very hesitant at this time, and he struggled for two days, and he didn't know how to report the result of Zhang Chen's refusal to cooperate.

The company gave him the right to get up to 5 billion soft girl coins, but he still didn't win the cooperation with Chenyue Biology.

He took a deep breath, exhaled slowly, and dialed out a cell phone number.

"Mr. Wei, then, what...... Are you busy? ”

After the phone was connected, Qian Youwei panicked.

This Mr. Wei is not easy to get along with, he is the head of Yi Lumina in China.

Qian Youwei has only met this Mr. Wei once, but that time made him remember it very well.

At that time, all the high-level meetings were held in the capital.

Just because when the general manager of North China reported his work, he said the wrong annual profit increase ratio, and the general manager Wei asked him to leave on the spot.

Now Qian Youwei has a huge sum of five billion in his hands, and with three cooperation plans, he has failed to win Chenyue's cooperation.

He had already anticipated his end.

"You say."

A deep voice came from the other end of the line.

Qian Youwei tried to calm his mood, and said tremblingly: "Mr. Wei, I'm sorry, I didn't get the cooperation with Chenyue Biology. ”

"I see, you don't have to worry about it."

Qian Youwei was a little confused, he held up the mobile phone that kept ringing "Dududu", and his mind was full of question marks.

Am I going to get out of here, or am I not going to have to get out?