Although Zhang Chen is doing a gene knockout experiment for the first time.

However, with the support of rich theoretical knowledge and the fast growth rate of the mouse model, two weeks is more than enough, and there is also plenty of time for error.

To solve the gene knockout problem required by the Beihe gene.

The first thing that came to Zhang Chen's mind was CRISPR/Cas9 gene editing technology, which is still the latest technology for existing biotechnology.

However, in the systematic version of "Molecular Biology", it has been systematically introduced as early as the basic knowledge node.

And until many years later, it was still a practical technology, and of course it had undergone a series of complex modifications.

The modified and optimized CRISPR/Cas9 gene editing technology can ensure a 100% success rate without the original high off-target rate, and there is no longer a size limit for knockout or knock-in genes.

Therefore, although the name has not changed, its technical content has long been incomparable to the existing CRISPR/Cas9.

Zhang Chen spent 50 points to directly exchange for the ready-made recognition protein and modification module in CRISPR/Cas9 gene editing technology.

This set of basic tools can be used in many future experiments.

And it's not just gene editing in experimental animals, crops such as rice and soybeans can be used in genetically level research.

Even the genetic modification of various other animals and plants will use this set of tools.

According to Zhang Chen's current level of knowledge, he can spend time constructing plasmid DNA and then translating and expressing the set of protein tools he wants.

But it would take at least two weeks, and now with the urgent task of Beihe Gene, Zhang Chen didn't save the 50 points anymore.

Complete the task early, make a mouse model, and let the reputation of the experimental animals go out, and the reward of points will be thousands of times.

Zhang Chen came to the laboratory and prepared to start a series of experiments.

He embodied humanized mice, superovulation to obtain better quality mouse receptor--sperm eggs, and added microinjection medium.

According to the knowledge learned in "Molecular Biology", the appropriate concentration of protein and the appropriate opening size of the injection needle are strictly selected.

Zhang Chen's hands did not tremble at all, and he grasped the timing and successfully completed the microinjection in just one time.

The CRISPR/Cas9 protein tool can be efficiently edited in the recipient sperm egg, which can accurately knock out tumor suppressor genes in mice without leaving traces of genome editing.

Zhang Chen concocted it according to the law and injected a total of twenty mice.

He introduced the injected fertilized eggs into the mice, and let them grow in an environment of constant temperature and humidity, free diet and drinking.

In the end, Wang Lele was explained and asked to personally supervise the animal breeders and take care of the twenty mice.

According to the system, this batch of mice only needs about five days to pass the gestation period, and can be weaned and live independently after a week.

Therefore, in about 12 days, Beihe Gene can be delivered and the task of the first order of experimental animals can be completed.

This flying growth rate surprised Zhang Chen.

The system is produced and must be a high-quality product!

He was eager to find out what genes were making this mouse model grow so much faster.

When you have time, use the system to simulate the basic principles.

When the time comes, we will extract this gene and see if it can be applied to other scenarios.

Zhang Chen's brain was open, if he could, he wanted to use this gene to engage in the breeding industry.

Or applied to the field of grain planting, which can also be regarded as a method of increasing production.

You can't rely on the system to exchange everything directly, you can save it.

The points saved are used to do other things that must be used, which is the reasonable allocation of resources.

Moreover, the things made by yourself are more fulfilling in Zhang Chen's opinion.

Can the cake I bought be the same as the cake I made by DIY?

Even if the taste is the same, the feeling of eating it will be very different, and the mood of the people who celebrate the festival will be very different.

Zhang Chen took one last look at his masterpiece, walked out of the laboratory with satisfaction, and came to Zhang Yue's office.

When Zhang Yue saw Zhang Chen coming in, she immediately asked, "How is it, your experiment went well, right?" ”

Zhang Chen replied with a confident smile: "Of course it's smooth, you still don't believe me?" Now you can tell Huang Ruifang that it will take about 12 days for her to get the first generation of mice, and she can start to form tumors directly after taking them. ”

After a pause, he continued: "Now you can let them change the experimental method of mice in advance, the growth rate of this batch of mice in our company is much faster than that of ordinary mice, for specific situations, I will send you a piece of information, and you can forward it to her." ”

"It's amazing, I admire you more and more," Zhang Yue looked up, her eyes full of little stars.

Zhang Chen smiled: "Wait, I will only get better and better in the future." ”

"Oh, narcissist~"

“……”

Zhang Chen returned to his office, sorted out some parameters of the mouse model, packed them and sent them to Zhang Yue.

After Huang Ruifang received the information forwarded by Zhang Yue, he studied it carefully as soon as possible.

After a few minutes, there was a hint of shock in her eyes, and then the shock gradually turned to excitement.

Even the fingers that slide the mouse panel are soaked with some sweat, which makes the fingers sliding on the panel very astringent.

But Huang Ruifang didn't have time to care about that, she was completely immersed in the world of information.

Half an hour later, she pulled out a few sheets of paper and wiped the sweat from her hands.

"It's really worthy of the morning moon creature, even the experimental mice are so perfect."

In the information sent to her by Zhang Chen, she not only introduced the growth cycle of mice in detail, but also attached some experimental results given by the system.

Including tumorigenic effects, validation experimental results of some existing common drugs, etc.

It can be said that with this information, it can be completely explained that the mouse model made by Zhang Chen does not have any defects in functional verification.

Huang Ruifang excitedly clutched the paper ball in her hand.

Animal experiments on oncolytic viruses are promising!

But immediately, she began to worry again, what if she used the mouse model of Chenyue Biology and still didn't get the experimental results she wanted?

This project, in Chenyue Biology alone, cost nearly 10 million.

It took 2 million to build a mouse model and millions to sequency.

In order to move forward quickly with the whole project, a large number of experiments were outsourced in other CDMO companies.

This oncolytic virus project alone has directly spent more than 50 million yuan in more than a month.

However, the company's first round of financing only raised 180 million.

Huang Ruifang took a deep breath, everything depends on fate.

It's really hard to make original new drugs.

I don't know how Chenyue Bio's gene therapy drug is made.

Could it be that a lot of technical reserves have been made several years in advance?

But I heard that Zhang Chen is a grassroots background, where did he get the technical reserves?

When she encountered difficulties in the project she brought, and when she thought of the efficacy of Chenyue Bio's gene therapy drug, Huang Ruifang's heart was as sour as if she had eaten a lemon.

People are more popular than dead people!