Chapter 122: Hefeng Gene
Early in the morning of August 20, Zhang Chen came to the company with his family.
He was very happy that the experimental animals were finally starting to improve.
Yesterday afternoon, Xu Zimo received a lot of phone calls, all of which came to consult experimental animals.
And they're all school students.
Seeing such a result, Zhang Chen was very pleased.
The company's other products are continuing to generate revenue, and the sales of sequencers have also improved, and many companies and institutions have called and have purchase intentions.
Now, the experimental animals are finally going to start exerting their strength, and Zhang Chen's expectations for this product are not small.
The advantages of the experimental animal model produced by the system are obvious, and what other companies can do, this is only the foundation.
What other companies can't do and dare not think, he can easily do.
And the growth cycle of all experimental animals has been shortened by three times, which will become the biggest selling point of this product.
The market for laboratory animals is huge, which is something anyone can imagine.
Pharmaceutical companies, scientific research institutions, vaccine research and development, universities, these are all market audiences.
In fact, there are not many companies that make laboratory animals in the country, and the geographical distribution is uneven.
With Zhang Chen's existing crushing technology, it is not too simple to seize the market.
The establishment of many disease models is very complex, and other companies simply can't handle it, and this market can soon be won by Chenyue Biotech.
At the same time, Zhang Chen was thinking about whether to publish a few papers, and he also wanted to get the title of academician.
If someone says that he is not good at academic qualifications, then he will do a few more papers, and if only the papers are not good, he will simply win a few more Nobel Prizes.
Zhang Chen, who has a scientific and technological system, if he can't even rely on academicians, then what kind of stars and sea are there to talk about.
It's not for show, it's mainly that if there is an academician in the research base, it will definitely attract a lot of talents to settle in.
However, there is no rush in this matter, and it is better to solve the soybean yield problem first.
Zhang Chen opened the points mall and looked at soybean-related technology products.
Soon, a simple introduction of a product instantly attracted Zhang Chen's attention.
[Hefeng gene, priced at 3000 credits, this gene is overexpressed, which can improve the photosynthesis efficiency and nitrogen use efficiency of all food crops, so as to achieve the purpose of increasing yields, and the estimated yield increases range from 61% to 98%. 】
The system can really sell everything, even genes can be sold as commodities.
What attracted him was the role of [Hefeng Gene], which was too comprehensive.
Its scope of action is all food crops!
Not only can you increase the yield of soybeans, but also corn, rice, and potatoes!
And the increase in production by 61% to 98% is a bit scary.
In Zhang Chen's opinion, the price of this product is 3000 points, which is very cost-effective and very worthwhile.
Even if you sell 5000 points, it's not too much.
He deliberately saved 500 points for profit yesterday, plus the points reserved before, there are just 3030 points, which can be exchanged for [Hefeng Gene].
Zhang Chen glanced at the other soybean technology products again, and then silently returned to the original page.
No way, not enough points.
Now the only thing that can be exchanged is [Hefeng Gene], which is considered a basic product.
But in Zhang Chen's view, such a degree of production increase can completely shock everyone.
Even if you take a median value, the increase in yield is 80%.
Even the lowest value is 61%!
And all food crops.
Cereals, potatoes, legumes, these are all food crops!
With this [Hefeng Gene], a new product is released every month, which can last for more than a year.
Zhang Chen will definitely not announce all the results at once.
He was a little curious about the specific working principle of [Hefeng Gene], and hurriedly called up a detailed introduction.
This is a Chinese researcher, by chance, found a transcription factor in rice that is both light-sensitive and low-nitrogen-induced: Hefeng factor.
Hefeng gene comes from this.
In fact, many people know that photosynthesis is the basis of all life matter and energy on earth.
Plants need to photosynthesize carbon dioxide and water into organic matter, so that carbon can be sequestered.
Nitrogen is an important component of chlorophyll, protein, nucleic acid and metabolites, and is a large number of elements necessary for the growth and development of crops.
The processes of photosynthetic carbon assimilation and nitrogen uptake and utilization are closely coupled, which are crucial for crop growth and yield formation, and the synergy of crop carbon-nitrogen metabolism is the basis for achieving high yield.
Hefeng gene overexpression can significantly increase the rate of photosynthetic carbon assimilation of crops, grow faster under light, accumulate more photosynthetic products in leaves, and accelerate the grain filling rate of grains or rhizomes.
At the same time, the overexpression plants had enhanced nitrogen absorption and transport capacity, and could allocate more nitrogen to grains or rhizomes, and the nitrogen use efficiency was significantly improved.
Hefeng genetics act as a catalyst that accelerates photosynthesis and increases the growth rate of seeds or rhizomes.
According to the information attached to the system, the yield of Hefeng gene overexpression plants has been much higher than the yield level of ordinary plants under the condition of nitrogen fertilizer, and the "nitrogen reduction and high yield" have been perfectly achieved.
At that time, Huaguo researchers also unexpectedly discovered that overexpression of Hefeng genes could shorten the entire growth cycle of crops.
And after the researcher discovered this gene, Chinese scientists have continued to follow up and study for hundreds of years, and finally proved that the gene has no effect on the human body.
After reading the information, Zhang Chen's face was full of shock, and he sighed at the magic of nature.
He no longer hesitated, and directly exchanged the [Hefeng Gene].
Zhang Chen hurriedly came to his dedicated laboratory and asked "Commander No. 5" to guard the door of the laboratory.
A burst of white light flashed, and Zhang Chen embodied the [Hefeng gene].
Looking at the 1.5 ml centrifuge tube in his hand, Zhang Chen felt very kind.
At the same time, I couldn't help but start complaining about the system.
Isn't a high-tech product with 3000 points worthy of a more advanced package?
Spit and complain, the work that should be done still has to continue.
What needs to be done now is to get more [Hefeng genes].
This gene fragment was cloned on a commonly used pUC57 plasmid vector.
Zhang Chen is extremely familiar with this set.
When he was doing plasmid construction at Borui Biotech before, he was dealing with this thing every day.
Now the plasmid vector containing [Hefeng gene] is transformed into competent cells containing E. coli for expansion and culture.
Then, it was coated on solid LB medium and screened and cultured under certain temperature conditions, and more plasmid vectors carrying [Hefeng gene] could be obtained.
Zhang Chen turned on the ice machine and started making ice.
Then, follow the instructions in the system, add 50 μl of water for injection to the centrifuge tube and mix well on the shaker.
After that, remove the competent cells from the ultra-low temperature freezer at minus 80 degrees Celsius and place them on ice.
After the competent cells thawed, Zhang Chen used a pipette to absorb 0.5 microliters of the [Hefeng Gene] cloning vector, beat it into the competent cells, and gently pipette to mix well.
Competent cells are then allowed to ice bath on ice for half an hour, then placed in a metal bath and subjected to 42 degrees Celsius for 1 minute, followed by an ice bath for another 2 minutes.
Finally, in a biosafety cabinet, a portion of competent cells is taken and coated onto ampicin-resistant solid LB medium.
After proper drying, cover the medium, invert it in a constant temperature incubator, and incubate it overnight at a constant temperature of 37 degrees Celsius.
Wait until this time tomorrow, you can get more [Hefeng genes].