Chapter 0025: I'm a Broken Wall of Hybrid Rice
Chen Cheng dialed back the two numbers with the most calls and figured out that they were all partners who came to negotiate technology transformation.
"Brother Kai, your job is here."
Wang Kai was carrying a soy milk bun in his hand and looked at him with a confused expression.
Chen Cheng said: "Don't you like to socialize, there are dozens of pesticide companies that need you to investigate and dock, at least 2/3 of them will be screened out first."
Wang Kai was pleasantly surprised: "Really? Watching Ding Tao and Tang Qiang do experiments with you every day, now I finally have a place for my buddy. ”
Chen Cheng asked Wang Kai to take his mobile phone and transcribe the calls that he didn't answer last night, most of them called many times, and there were more than 20 pesticide companies in total.
He asked Ding Tao to help send the technology transformation plan to the principal's office, he really didn't have time to report to Zhao Feng personally, so he had to do Zhou Kai's experiment as soon as possible.
Zhou Kai's experimental rice has reached the flowering stage, and he hurried a little to catch up with this pollination.
Chen Cheng taught Wang Kai how to research and screen potential partners while eating breakfast, and after eating, he turned around and went into the room to start the experiment.
Calling up the super agricultural technology laboratory, he leaned the male flower slide prepared by Wu Yongping into front of his eyes.
【Male pollen cells of hybrid rice restorer line were detected】
[Name: Oka type sterile No. 4 restorer line]
[Gene sequencing: This pollen cell contains all dominant dominant genes and all inferior genetic traits of gang-type sterility]
The sterile line of hybrid rice refers to rice plants with defective pollen from male flowers that cannot give gold to their own female flowers.
Restorer lines are rice plants in which male pollen is able to give gold to their own female flowers and other female flowers.
The process of hybridization is to use the rice of the restorer line to pollinate the rice of the sterile line to achieve the dominant gene combination of the two.
Now Chen Cheng is holding the pollen of the restorer male flower that can give gold to the female flower.
"Detect all traits in the gene."
As soon as he finished speaking, the supercomputer began to calculate, and in less than a minute, all the traits of this breed were listed.
[Dominant traits: increased yield, insect pest control, drought tolerance]
[Disadvantages traits: long growth cycle, hemp rod disease, borer disease]
That is, gene expression for these disadvantaged traits needs to be limited.
"Gene combinations for dominant and inferior traits are listed separately."
The supercomputer quickly gave all the genetic combinations of the breed and named them with simple letters.
There were eight kinds of AA, AB, Aa, Ab, BB, Ba, Bb and BB, among which the dominant genes were AA, AB and BB.
"How thick is the pollen cell wall of the male flower of this variety? How thin does it have to be for it to break automatically under natural conditions? ”
[The current cell wall thickness is 0.3 μm, below 0.1 μm, which can be dehydrated under short light exposure and rupture in slight shaking]
"Okay, let's start iterative culture experiments to reduce the cell wall thickness of all inferior gene cells to less than 0.1 μm."
【Okay.】 Will 1 number of experiments be consumed, do you want to continue? 】
"Go on."
The culture chamber of the super laboratory is opened, a batch of 3D digitized pollen cells are placed in it, and then the incubation begins.
Unlike last time, this time it was carried out directly in a petri dish in the culture chamber, and it did not develop into seeds and rice plants at all.
This drastically reduces the time it takes to iterate nurturing.
Chen Cheng watched as the cell mass in the petri dish continued to grow in size within a few minutes, and then a robotic hand took out a few drops with a straw and put them into another petri dish to start the next generation of pollen cell culture.
After almost ten times, the chamber stopped working, and a dish full of cells was removed.
"This should be the iterative pollen cell, right?"
The supercomputer loads the information of the cultured cells, starts the test calculation, and after ten seconds, the experimental results are given.
[The cell wall thickness of the inferior genes Aa, Ab, Ba, Bb, and bb has been reduced to less than 0.1 μm, and this characteristic has been hereditary]
"Beautiful!" Chen Cheng shouted happily.
The experiment completely achieved his goal, that is, the offspring bred by this pollen, as long as it is a disadvantage gene, will definitely have the characteristics of parenchyma cells. It perfectly realized the technical route he said to Zhou Kai.
Chen Cheng excitedly extracted the improved pollen information into reality and passed it into his biological replica machine.
The panels of the bio-replicators show that the raw materials needed to replicate pollen are all available in their lab.
Looking at the remaining 8 reproductions on the panel, Chen Cheng gritted his teeth and clicked 2 times.
Give Zhou Kai some more pollen so that they can breed enough first-generation improved hybrid rice seeds this time.
Two hours later, two bags of pollen were dropped out of the replica machine.
While he was waiting, he opened a phone call with Zhou, and learned that the sterile rice flowers in the experimental field were in full bloom, so he decided to take a taxi to send them the pollen.
ran downstairs, but saw that Zhou Kai's full-time driver and S300 were already waiting for him.
The driver drove very fast, and it took more than an hour to send Chen Cheng to the experimental field of Han University.
The provincial capital of Hanxi is at a premium, and their experimental field is naturally in the suburbs, but their experimental field is surrounded by a high wall, and there is a roof that can be automatically retracted.
This can well avoid the impact of bad weather on the experimental field.
Zhou Kai's health was not so good, so he set up a chair and sat on the ridge to wait for Chen Cheng, and Wu Yongping held an umbrella for him next to him.
"Xiao Chen, you're finally here!" Zhou Kai got up to greet him.
Chen Cheng hurriedly walked over.
The weather is so hot, he is afraid that Zhou Kai's body will not be able to bear it.
Zhou Kai said apologetically: "Xiao Chen, please hurry over here, haven't you had lunch yet?" ”
Chen Cheng said with a smile: "Today, the sun is big, the temperature is high, and the rice flowers are blooming brightly, which is a good time for pollination." There is no rush to eat. ”
Zhou Kai looked at the experimental fields, the ears of rice rustling in the breeze.
"yes, pollinating them is more important than our meals."
Indeed, those who are engaged in scientific research in the field have to follow the habits of the experimental subjects in all their work and rest time. In order to conduct experiments, it is common to go hungry.
A few assistant researchers over there had already put on their boots and were waiting at the edge of the field, Chen Cheng didn't dare to delay, and hurriedly handed two packets of pollen to Zhou Kai.
"You cultivated two bags of pollen so quickly?" Zhou Kai was surprised.
Chen Cheng nodded: "The cell wall thickness of all the inferior genes has been reduced to 0.1 μm, and they have heritable characteristics. ”
Zhou Kai was so surprised that his feet were weak and he almost fell. Chen Cheng hurriedly helped him up.
He held two matchbox-sized bags of pollen, his hands trembling slightly.
"It will take five years to achieve the improvement goal of ten generations of rice cultivation in one day?"