Chapter 0037 Design and production process
Everyone else was busy, and Chen Cheng was not idle, and began to sort out the mass production plan of super urease microorganisms.
The mass production solution of the product level is certainly very different from the research preparation technology in the laboratory. The most obvious is the preparation process.
In the laboratory, the amount of sample prepared in one dish is sufficient for research use, but to make a product, the production process must be redesigned according to the target yield.
The standard production process includes the improvement of production equipment, the development of mass production technology, production process management, etc., if in accordance with the standard ISO 9001 quality standard system, it has to be a huge and complicated work.
But for Chen Cheng now, it is enough to design a production process to produce super urease microorganisms.
As for the quality standards or something, they will be gradually improved later.
According to the description of the system, the production of super urease microorganisms only requires placing them in a container large enough to allow them to multiply freely.
The microorganisms are characterized by a larger, heavier nucleus of mature somatic cells that aggregate at the lower end of the culture vessel.
Therefore, Chen Cheng needed to design a funnel-shaped culture vessel that could continuously discharge the mature super urease microorganisms.
He drew a rough diagram on paper, a large, deep barrel-shaped hole-shaped culture vessel with a feed port for adding straw powder and a pipe for adding the culture liquid.
The discharge port of the culture vessel has a valve to control the speed, and according to the design, the mixture of culture broth with a high content of urease microorganisms is discharged from the valve.
At this time, the content of the culture medium is already very small, and it will be consumed by microorganisms during transportation, and there is no problem of leaking technical secrets.
The optimal temperature for the reproduction of super urease microorganisms is 24°C, although it is not much different from the current ambient temperature, but Chen Cheng still designed a temperature sensor inside and a circulating water cooling device outside.
Because the heat will be emitted during the reproduction of microorganisms, if it is not transmitted in time, the temperature of the culture medium will rise, which is not conducive to the rapid reproduction of microorganisms.
Of course, the design of this production process device, Chen Cheng adheres to the principle of simple structure and optimal effect.
After all, now they need to race against time, the process equipment is too complex to design, and it takes many days to process and manufacture.
After Chen Cheng got all the designs, Ding Tao, who bought the ingredients for the culture liquid, also came back.
Chen Cheng took a picture of the design he had just drawn and sent it to Wang Kai, so that Wang Kai could personally urge Huang Weidong to manufacture new equipment.
This device does not involve high temperature and high pressure, it is ordinary stainless steel plate welding, as long as the money is enough, it is no problem to process it in one day.
At the same time, Wang Kai reported that the first batch of straw purchased there has arrived in the factory and is being arranged to be ground into powder.
"Ah Tao, go find clean plastic buckets with lids, at least 4 of them."
Ding Tao, who hadn't had time to rest, immediately turned around and left.
Here, he arranged for Tang Qiang to extract pure water, and Tang Qiang also took action immediately.
These brothers in the dormitory have nothing to say about their mobility, and they do what they say, without dragging their feet.
Chen Cheng began to weigh and prepare the ingredients for the culture medium with a balance. There are calcium-containing protein powder, potassium chloride, phosphoric acid, zinc acid, etc.
After weighing and preparing according to the proportion, it was packed into a plastic bucket that Ding Tao found. Dissolve well in a plastic bucket and then mix into four parts.
Here, Tang Qiang has already brought purified pure water.
There are four different grades of pure water in the laboratory, which are pure water, deionized water, laboratory grade II pure water, and ultrapure water. Different types of pure water are usually distinguished and detected based on conductivity.
Microorganisms are generally cultivated using laboratory class II pure water. Laboratory grade II pure water has a conductivity of < 1.0 us/cm, a TOC content of less than 50 ppb, and a bacterial content of less than 1 CFU/ml.
The water quality of laboratory grade II pure water can meet the needs of a variety of experiments, and is usually used for solution dilution, bacterial culture with nutrient solution, microbial research, etc.
However, Chen Cheng only uses pure water of deionized water grade. Because the sterile environment of the laboratory can meet the sterility requirements, but the water will still be contaminated when it comes to the production workshop.
Simply lower the requirements a little and sacrifice a little bit of the activity of the super urease microorganism.
After mixing the culture medium and measuring the PH value within the range, the three people who were already drenched in strength covered the lid and sat down to rest.
Preparing the culture medium is indeed manual work and requires thorough stirring, especially with a total of 4 buckets of 100 liters.
"Lao Chen, when will Brother Kai finish the cultivation container?" Ding Tao asked wiping his sweat.
Chen Cheng said: "If you work overtime, tomorrow morning at the earliest." ”
"Well, we'll have to carry these four vats downstairs and load them tomorrow." Tang Qiang said with a tired face.
Chen Cheng said: "Hard work brothers! When I'm done with this, I'll treat you to a big meal. There are still a few thousand dollars left from the scholarship last time, hehe. ”
Tang Qiang explained: "It's not that I want you to invite you to dinner, but why don't we take this thing to the scene to prepare it?" So much water, it's too heavy! ”
Chen Cheng said: "This culture medium is our technical secret, and we can't let others easily see the preparation process." Even at the production site, you have to let Kai Ge guard. ”
Ding Tao and Tang Qiang both nodded as if they didn't understand.
How can the culture medium become a technical secret when cultivating microorganisms?
Tang Qiang suddenly had a flash of inspiration and asked, "Since we are in such a hurry to produce, why don't we put the microorganism in it now and let it start to multiply?" ”
"That's right! Ah Tao, you have a good idea! ”
Chen Cheng waved his hand and said, "If you put them in now, you don't have to wait until tomorrow morning, these four barrels will have to explode in two hours." ”
"Is it so powerful?!" ×2
Chen Cheng nodded.
When Ding Tao went to the cafeteria to eat, Chen Cheng cleaned up the laboratory, and Tang Qiang took out his mobile phone to watch the news.
Suddenly, he stopped Chen Cheng: "Old Chen, come and see this news." ”
Chen Cheng poked his head over and saw the news headline on his phone:
"Hanxi Province has been dry for 20 days after autumn, and there is no harvest of soybeans this autumn!"
Chen Cheng hurriedly wiped the water off his hands, took Tang Qiang's mobile phone, and looked through the content below.
The news mainly said that there are about 400 acres of autumn soybeans planted in Hanxi Province this year, and it is time to fertilize and grow, but the weather is dry, and most of the fertilization will be lost.
The reporter interviewed a farmer, who said with a sad face: "If it doesn't rain for a few days, he will shovel the soybean seedlings and prepare to plant wheat." ”
Finally, the reporter concluded that some agricultural experts predicted that the reduction of soybean production this autumn has become a fact, and called on farmers to change wheat as soon as possible to reduce losses.
"Lao Chen, I didn't expect the impact of the drought to be so severe this year." Tang Qiang said with a gloomy expression.
Chen Cheng silently put down his phone. The depressed and poignant face of the farmer in the news photo just now lingered in his mind.
"We have to get the product to market as quickly as possible!" He said secretly.