"What's wrong with Senior Brother Wang today? Why do you seem to be a little unhappy? "In the evening, eleven o'clock.

Fang Ziye had time to come to the laboratory again to take a look at his cells and ask Luo Tingzhu.

But at this time, the laboratory was still brightly lit, and the number of people was not small. The academic master's and doctoral students in Professor Deng Yong's group are still busy with their own work.

Even professional doctors, such as Senior Brother Wang Yuanqi, came to the laboratory to do some cell experiments after leaving the practice room.

"I've just been here for a while, but I heard Senior Brother Yan Zhiming say that it seems that Senior Brother Wang's flow style has failed, and I'm in a bad mood." Luo Tingzhu is excerpting the experimental operation process at the moment.

The first time you come into contact with a new experiment, even if you watch the teaching video many times, you need to copy the experimental process again to avoid forgetting.

"Failed?" Fang Ziye really wanted to take a look, but he didn't dare to touch his head for knowledge.

"Actually, it's normal to fail, but Senior Brother Wang's flow has failed three or four times, wasting a lot of money." Luo listened to Zhu say this.

"How much does it cost to do a stream?" Fang Ziye asked.

Since entering the laboratory, Fang Ziye will be very concerned about the price, so a bottle of fetal bovine serum, four or five thousand is only five hundred milliliters, this price, even after dilution into culture medium, is still a dollar and a milliliter!

Does this still stand up to reckoning?

"I don't know, do I?" Luo Tingzhu flipped his hand.

Oh, Fang Ziye also forgot that Luo Tingzhu is also a little budding newcomer in the laboratory, but he is not so cute as himself.

"Have your cells grown?" Fang Ziye changed the topic, Luo Tingzhu had just recovered yesterday, but the cells he had recovered were growing well, and almost one-fifth of them were attached to the wall.

In two or three days, a portion of it can be harvested and cryopreserved, and then the passage will begin the next step of the experiment.

"It's okay, I had osteosarcoma, and a third of it was adherent. It's growing pretty fast. Luo Tingzhu breathed a sigh of relief.

Fang Ziye was very envious: "It is worthy of being an orthopedic tumor cell artifact osteosarcoma, which grows much faster than the giant cell tumor of bone I raised." ”

Different cells have different growth cycles and division cycles, and there is no feed to feed this thing. Fang Ziye can only be envious.

"It's okay, just grow slowly."

"Can I borrow your notebook and let me copy the protocols for the scratch experiment?" Fang Ziye asked.

The scratch test is an experiment to record the function of cell metastasis, which is equivalent to letting tumor cells in different treatment groups run into the blank space from the first day.

Whoever runs faster will have a stronger ability to transfer.

According to the prediction, the overexpression of HK2 should be greater than that of the normal group and greater than that of the silent group.

Overexpression is equivalent to an athlete taking doping, the normal group is a normal rest, and the silent group is equivalent to an athlete who stays up for ten nights before participating in the 100-meter race, and then fires hundreds of rounds for two days, running the slowest speed.

It is an experimental method to detect the transfer ability of cells in different treatment groups, and it is also a relatively simple method, easy to operate, and is mostly a kind of proof operation for the first time in the laboratory.

"Yes, I'll give it to you when I'm done copying." Luo Tingzhu nodded, and then continued to transcribe the steps of the cell invasion experiment word by word.

To verify the invasion ability of tumor cells, there are other experimental methods.

……

When Fang Ziye was waiting for Luo Tingzhu's experimental notes, what he didn't expect was that Senior Brother Wang Yuanqi walked over curiously first: "Ziye, when did you come to the laboratory?" ”

Fang Ziye is looking at the specific steps of the scratch experiment and the relevant preparations for dyeing and photos.

Hearing this, he hurriedly put down his mobile phone: "Just came in, Senior Brother Wang." ”

Wang Yuanqi smiled and said, "I just said why didn't I see you in the practice room today, it turns out that after you ascended, you came here?" ”

"Isn't this learning from your steps, Senior Brother Wang. You flew earlier. Fang Ziye hurriedly moved a stool for Wang Yuanqi and sat down.

The person in charge of this laboratory is Professor Deng Yong, and the laboratory was also applied by Professor Deng Yong from the hospital, so in fact, whether it is Yuan Weihong or Fang Ziye, they are currently following Professor Deng Yong.

Senior Brother Wang Yuanqi is a second-year doctor, and his operation is more awesome than Fang Ziye's current operation, although it is not much, but it is always more powerful, at least what he wants to target is the requirements of the doctor's "practice room".

Fang Ziye didn't dare to covet such a far away at the moment.

"Are you going to do a Viagra experiment? How's the progress? Wang Yuanqi probably didn't have the heart to care about anything else at the moment, so he came over to talk to Fang Ziye with his watch.

"The cells were revived yesterday, and they haven't been frozen yet, so it's very early, Senior Brother Wang."

"It's okay, everything is difficult at the beginning, take the first step, and then get used to it. If there's someone you want to help, if I'm free, send a message or something, don't get too angry. Wang Yuanqi is still quite friendly.

may also know that Fang Ziye will still enter Professor Deng Yong as a junior disciple in the future, which can be regarded as warming up the relationship in advance.

"Thank you, Senior Brother Wang."

Wang Yuanqi didn't sit for long, and went to clean up in frustration, even if the experiment failed, what should be cleaned up should be cleaned up, the experiment is full of too many uncertainties, and most of them are new directions, other people's experimental methods can only be used as a reference.

After Luo Tingzhu finished copying the experimental steps, he said, "Senior Brother Fang, if you don't want to take it back and copy it, it's not too early." I'm going back first. ”

"Okay, I'll go back too, let's go together?" Fang Ziye invited.

Luo Tingzhu nodded.

The two of them walked out of the laboratory door together, without attracting any attention, they were brothers and sisters or sisters and brothers in the laboratory, what was there when they walked together?

And it's not too early for this point, those who are still in the laboratory, and the cell babies who are all concerned, who will care about other people and who will go with whom?

Entering the laboratory today, I didn't get the knowledge point, although Fang Ziye was a little disappointed, but when he pressed the elevator and waited, Fang Ziye didn't let the atmosphere chill: "Senior Sister Luo is not far from the doctor's exit requirements in the practice room, right?" ”

Luo Tingzhu didn't refute: "But if you stay in the hospital, you will almost accumulate scientific research." At present, the number of hospitalizations in the orthopedic department of our hospital is very volume, and there are basically no one or two treatise-type articles in the first district, so don't even think about it. ”

"Senior Brother Sun Shaoqing is the basic article of the treatise in the first district, because a senior brother in joint surgery has three articles. And it's not far from the requirements for the doctor's practice room. ”

Fang Ziye was shocked when he heard this.

Good guys.

In the session of his teacher Yuan Weihong, you can stay in the hospital when you leave the customs practice room, and now, the articles of the type of treatises in the customs practice room plus a district may not meet the standards for staying in the hospital?

"I believe that Senior Sister Luo, you have good talent, and you can definitely find a good job." Fang Ziye cheered Luo Tingzhu on.

Luo Tingzhu shook his head again: "Senior Brother Fang, then you are thinking wrong again, Professor Deng here?" ”

"The number of projects is not enough, I have to figure out my own way, if I can write a bid next year, and then get the general fund, I will have the funds to do experiments, otherwise?"

"Actually, I'm very envious of you, Senior Brother Fang, you directly have the money to do experiments......

"Huh?" Fang Ziye was stunned.

"Senior brother, not every doctor has the money to do experiments. I'm living in a dormitory, so you shouldn't live in a dormitory, right? After getting off the elevator, Luo Tingzhu asked.

"I rented a house outside." Fang Ziye explained.

The two of them said goodbye.

It's just that after chatting with Luo Tingzhu like this, Fang Ziye suddenly found that the advantages and disadvantages of following a young mentor are actually not so obvious, as long as he can cross the hurdle of studying for a doctorate.

After all, after studying for a doctorate, he is still a student of Master Yuan Weihong, Yuan Weihong's topic, Fang Ziye can also use the money in it to do experiments, and the output of scientific research is still Yuan Weihong's, but he borrowed the identity of Professor Deng Yong's doctoral supervisor.

Instead of directly sharing with Yuan Weihong.

Yuan Weihong is a young tutor, and the premise of taking students is to win the general project or the National Natural Science Youth Fund, and there is money, time, and extra articles to ......

In that case, other senior brothers, then I'm not welcome?