One thing to say, the medical basic cell experiment technology package is really easy to use. It contains all the operations of common cell experiments.

After all of them were promoted to level 2, Fang Ziye's proficiency in the corresponding level was at least no less than that of ordinary mature academic master's students.

Let's just make an analogy.

Fang Ziye has been working diligently in the practice room for two years, and the basic medical operation skills have only reached level 2, and the level 2 proficiency in medical basic cell laboratory technology is equivalent to the proficiency achieved by an academic master, who has been conscientiously raising cells in the cell room for two years.

Yuan Weihong is a professional graduate student, with a master's degree and a doctorate, but in fact, he has not been in the laboratory for a long time, so his corresponding proficiency is not very high.

Fang Ziye went downstairs to buy another pair of shoes for Luo Tingzhu, and then bought another pair of shoes for himself.

There's no way, the teacher is here, his slippers have retired, and he used to wear a few random clothes when he entered the laboratory for a while, but now the students are in the laboratory, and they just wear Fang Ziye's shoes.

Fang Ziye accidentally put Luo Tingzhu's shoes on his feet, Yuan Weihong returned the shoes, Fang Ziye didn't dislike it, but if he returned the slippers, the girl might not think so.

So simply, Fang Ziye threw away Luo Tingzhu's slippers and bought two pairs again.

Lost Luo Tingzhu's shoes.

"Do you really do, or do you fake? These preliminary experiments are to be used as application topics, and there can be no mistakes. What if you won't? Yuan Weihong reminded Fang Ziye before it was about to start.

"Master, why don't you go back first and come over to check when you get to the point?" In order to make up for his previous mistakes, Fang Ziye wanted Yuan Weihong, the teacher, to take a break.

In general Transwell chamber experiments, the chamber is usually called the upper chamber, and the cell culture plate is called the lower chamber, and the bottom of the chamber is a polycarbonate microporous membrane, and a layer of Matrigel gel (Matrigel gel) is placed on the membrane.

Matrigel is a matrix component extracted from mouse sarcoma rich in extracellular matrix proteins, the main components include laminin, type IV collagen, nestin and many growth factors, Matrigel will coagulate into a biologically active three-dimensional matrix at room temperature, which can mimic the extracellular matrix, similar to the in vivo metastasis of tumor cells, cells with invasive ability will secrete matrix metalloproteinases to break down Matrigel, and further transfer outward.

At this moment, Fang Ziye has completed the dilution of serum-free medium and Matrigel, and is currently in the ice bath operation.

In this operation, Matrigel is diluted in a ratio of about 1:8 using serum-free medium on ice, added to the upper chamber at 100 μL/well, spread on the bottom, and placed in a cell culture incubator for 1-4 hours.

In this waiting process, there is no need for Yuan Weihong to wait here.

But after four hours, Fang Ziye and Luo Tingzhu need to wait here. It's relatively early.

The layman looks at the doorway, the insider looks at the excitement, Yuan Weihong saw that Fang Ziye's operation proficiency was okay, so he looked left and right, and nodded.

"When you do things, be a little more careful. ”

"If you don't understand, learn from your junior sister, ask more, and ask more questions without you spending money." Yuan Weihong admonished, then left with his hands behind his back.

Before Yuan Weihong left, Luo Tingzhu turned his head a little nervously and asked, "Senior Brother Fang, have you also entered the laboratory before?" Why are you so proficient in these operations? ”

As Yuan Weihong said, her experiments are preliminary experiments for applying for projects, and there is not much funding, so she can't tolerate too many failures, otherwise she won't ask Yuan Weihong for help.

It's just that Luo Tingzhu didn't expect that Fang Ziye, who was more budding than himself, would have such a high level of proficiency.

But it is estimated that it is new on the surface, and it is possible that Fang Ziye has secretly entered the laboratory before.

After all, I'm in my third year of master's degree, and I've been in orthopedics for so long.

There is a process of knowing how to do it unfamiliarly, and then to maturity.

Fang Ziye looked at the 4*6 twenty-four-hole cultivation plate, there was no big problem, Fang Zi also replied: "I've seen it a few times, but it should be fine, Senior Sister Luo, take advantage of this time, let's go to the practice room." ”

"It's okay to just wait here, the operation time you made today is relatively long, right?" Fang Ziye asked.

Whether it is the operating table or the cell operation room, you need to make an appointment.

Luo Tingzhu nodded, she must have taken care of her own affairs.

"Then it's no problem, let's go to the practice room together, after four hours, come over and add serum-free medium and complete medium, and then continue to culture for 24-48h." Fang Ziye is familiar with it.

It is also the success of the previous scratch experiment that gives Fang Ziye a certain confidence now.

"Good!" Luo Tingzhu saw that Fang Ziye was not worried, and his mood was slightly relieved.

Fang Ziye is not a Meng Lang person, and his carefulness will not be inferior to himself. Before helping Fang Ziye secretly take pictures, in fact, Luo Tingzhu did it easily, and he didn't dare to remind Fang Ziye.

After all, Fang Ziye's own record of the time node has an article as a reference. Standardized records and photographs, just to have more experimental materials, have no special meaning, and will not appear in the article.

If there is literature support, it may be that the editorial department will not ask for pictures with no reference significance, but Yuan Weihong's expectations for Fang Ziye are relatively high, so the requirements are slightly higher.

When they walked to the practice room together, Luo Tingzhu asked a little worriedly, "Senior Brother Fang, will you apply for school-level and city-level projects?" ”

Fang Ziye is also a master's student and a student at Han City University.

Luo Tingzhu can apply, Fang Ziye's qualifications are not bad at all, and Fang Ziye has a basic type of article guarantee, stronger ability, plus higher operation proficiency, and the preliminary experiment can be done better.

If there is a hedge, Luo Tingzhu estimates that he may not be able to rush Fang Ziye, and only hopes that Fang Ziye can choose only one of them.

"I think I'll try it out and do some projects during my PhD." Fang Ziye replied from the heart, but after seeing Luo Tingzhu's face change slightly, Fang Ziye said again: "Senior Sister Luo, as long as the quality of the subject is good enough, there will definitely be no problem." ”

"There is no rule that the fund will only fund one orthopedic-related topic."

Fang Ziye knew that Luo Tingzhu needed funds to do experiments, and only by doing a good job of experiments and publishing articles could she stay in the hospital.

Luo Tingzhu smiled and shook his head at Fang Ziye. It looks very generous, but his body curls up slightly, and he doesn't know if it's fear, or a subconscious defense.

And even after seeing Luo Tingzhu's performance, Fang Ziye's heart did not fluctuate much.

Luo Tingzhu needs a project fund to do experiments, and Fang Ziye also needs to have a project completion experience to thicken his resume, everyone is an ordinary person who is running around, who dares to have mercy and say what chance I will let you?

And Luo Tingzhu didn't speak all the way, and after entering the practice room, he said to Fang Ziye that she would practice first, and then the two were in place.