Naturally, these preparations are not only the ones that Fang Ziye is communicating with him now.

Some of the other arrangements are just conversations, not written records.

For example, Fang Ziye has planned for the next year, and it is expected that before April, he will complete all the experiments of his application topic, and then submit the manuscript, and strive to publish a thesis during the doctoral period and complete the basic thesis requirements for doctoral graduation.

At the same time, the research results of other more in-depth topics are used for their own general project applications and Fang Ziye will become a postdoctoral fellow next year to see if he can apply for the preliminary research results of municipal or provincial projects in his own name.

The relatively simple basic experimental data is the basic material used for the junior Jiehan to apply for school-level and municipal-level projects, if once it bursts out, then their own research group, starting from next year, will have four special funds for projects.

It is estimated that there may be 500,000 research funds, and at that time, the money in the group will be relatively rich, and with so much money, Fang Ziye will be able to do larger experiments, more operations, and more in-depth research on basic molecular science and even genetic epilogy......

Fang Ziye has really worked hard and planned for the future, and it is precisely because of this that Yuan Weihong is reluctant to let him work for others.

How can such a good student rest assured that he doesn't have it with him?

Naturally, the road to becoming a Qianqing or Jieqing in the case of working for yourself will be relatively smooth.

"No, I can't just ask Ziye to ask for money, I have to ask the hospital for a little research funding." Yuan Weihong, with a twist of thought, muttered so lowly.

In the hospital, there are special research fund rewards, and if you do well academically, you can be given a lot of project start-up funds and research special expenses.

As long as you have enough output, it is not impossible to start with a doxxing of 100,000 and a ceiling of 10 million for research funds.

Yuan Weihong plans to glance at here to see if he can get some money, don't always let Fang Ziye rub here and there, if you don't need to rub, buy what you want to buy openly, so that Fang Ziye can show his skills even more?

As a teacher, building a higher and better platform is especially beneficial to the development of students and teams.

For example, at present, the platform of the laboratory was built by Professor Deng Yong, so that Fang Ziye can have the background of Fang Ziye's current rubbing, otherwise, you are alone, you don't even have a laboratory, you do a fart experiment?

……

After Fang Ziye finished chatting with Yuan Weihong for the penultimate sentence, he didn't have time to reply to the master.

Because Luo Tingzhu walked out of the cell room at this time and said, "Senior Brother Fang, can you help me take a look, is there a problem with this exchange cell line?" ”

"Questions? Is there a problem with 143B or a problem with the HuO9 cell line? Fang Ziye's eyes flickered.

These two cell lines, but Luo Tingzhu has been running around for so long to get them, if there is a problem, then the other party is too pitiful, right?

"It's the second cell line, and I don't think it's quite right, but it doesn't seem to be pollution." Luo Tingzhu just changed his shoes, and he didn't take off his mask and hat.

When Fang Ziye heard this, he quickly put on his mask and hat, his eyes narrowed quickly, and walked into the cell room with Luo Tingzhu.

At this time, Luo Tingzhu put both kinds of cell culture dishes under the microscope, among which the type of 143B is very similar to the appearance of osteosarcoma, but it is true that the other cell line, the HuO9 type osteosarcoma cell line, is slightly different from the appearance of the common osteosarcoma cell line.

Although Fang Ziye does not make osteosarcoma cells himself, he also understands the appearance characteristics of osteosarcoma cells.

Oval or fibrous: Osteosarcoma cells typically have an oval or fibrous shape, which allows them to attach to the surface of the dish and form a single or multilayer of cells.

Mesenchymal-like features: Because osteosarcoma usually originates in bone tissue, osteosarcoma cells sometimes exhibit mesenchymal-like morphology, including elongated, stellate, or spindle-shaped.

Variation in nuclear size and shape: The nucleus of osteosarcoma cells may show variations in different sizes and shapes, and this nuclear heterogeneity may be one of the features of osteosarcoma.

Protrusions and pseudopodia: Osteosarcoma cells may develop protrusions and pseudopodia in culture, which aid in their movement and attachment on the dish.

Heterogeneity: There may be some morphological differences between different osteosarcoma cell lines. This difference may be a reflection of the osteosarcoma subtype, as osteosarcoma can exhibit a variety of subtypes, including well-differentiated, poorly differentiated, etc......

But in this cell line, it doesn't seem to have the appearance of osteosarcoma, and it looks relatively round.

Moreover, because Fang Ziye had exchanged liquids with Luo Tingzhu before, he may have participated in a part, at this moment, Fang Ziye just saw this thing, and he seemed to be floating like crazy in front of him:

“+1！”

“+1！”

“+1！”

“……”

This kind of continuation lasted for more than twenty seconds, and every second, the continuous speed was 2-3 points, which was more than forty or fifty points of knowledge, and suddenly it was directly poured into Fang Ziye's plus point panel.

Very bald.

Fang Ziye looked left and right on the spot, a little confused, and the last place where his gaze stayed was the neck of Luo Tingzhu, who was standing.

However, Luo Tingzhu didn't feel that Fang Ziye's gaze at the moment was aggressive, and Fang Ziye's blank expression really confirmed her guess.

It's just that Luo Tingzhu asked with a little loss and nervousness: "Yes, senior brother?" This cell line should not be a cell line of osteosarcoma, I don't know what it is. ”

"That guy got me the wrong one, right?"

Fang Ziye looked at the adherent growth results of another 143B cell line, which was somewhat similar to the HOS cell line that Ziye had seen before, but in terms of details, it was slightly different, and it may be a different subtype.

Fang Ziye stood up: "Maybe it's a mistake, this cell line that doesn't quite look like osteosarcoma, and even osteosarcoma may not be." ”

"Take a picture and ask her about it."

"Don't just throw the petri dish away." Fang Ziye later added.

Good guy, I don't know what this thing is, I just watched it for more than 20 seconds, just watched its cultivation, and increased so many knowledge points, this must not be simple.

Fang Ziye really wanted to leave it rooted, and then do some intervention to see if he could do anything to gain richer knowledge points.

At present, it is only cultivated, and after looking at the form of the adherent, if it has been treated, Fang Ziye does not dare to imagine.

Fifty points of knowledge, one-tenth of level 4 skills.

The awesomeness and power of the level 4 skill speaks for itself!

"Senior brother, I definitely won't throw it, but this shouldn't be an osteosarcoma cell line, right, it's not that I'm wrong." Luo Tingzhu pointed to the petri dish and asked.

In fact, what kind of cells is such a cell line, you can get the results by doing a genetic test or sending it to the company to make a comparison of the cell bank.

But with such an expense, it is better to buy a few new cell lines, how can Luo Tingzhu waste such spare money?

The research group will not be so wasteful.

"Probably not, you take a photo first, ask what the hell is going on with that person, and then find a common cell form of HuO9 from the Internet, find it on the company's official website, and send it to her."

"Maybe she's mistaken, but if it's wrong, we'll think of something else."

If this is really wrong, then go to Shashi to get it again, it is too twists and turns, Fang Ziye thought, it is better to find it in several hospitals affiliated to Huazhong University of Science and Technology or the orthopedic department of the Provincial People's Hospital.

Luo Tingzhu does not have relatively rich student resources, but Fang Ziye has a lot of student resources, and some people have been admitted to other universities in Han City from Tianjin Medical University, and some have studied in these places such as Modu.

Maybe you can borrow a cell line from them and send it by courier.

Because I know, I am also relieved.

Luo Tingzhu hurriedly took a photo and then asked.

I didn't delete my friends.

And, after Luo Tingzhu sent a message, the other party replied: "Huh? How is this cell, that may be that I took it wrong. ”

"So what now? Do you still come to Shashi to get it? The other party asked.

Luo Tingzhu showed the information interface to Fang Ziye, and his expression was full of gloom.

"Senior brother, it's really the other party who took it wrong." Luo Tingzhu sighed.

Fang Ziye said: "Why don't you ask the other party, what kind of cell is this, and what kind of cell is it?" I can't help it if I get it wrong, it's like this now. ”

"If it's a cell line or cell line in our orthopedics, we'll cryostore it first, and maybe we'll be able to use it later."

The inventory of cell types and quantities in a laboratory is actually a kind of heritage.

There are many cell experiments that can be done in orthopedics, such as inflammatory cells, giant cell tumors of bone, chondroblastoma and so on......

Even if it is not an orthopedic cell line, it can be preserved first.

Fang Ziye is easy to use by himself, anyway, he has cultivated it now.

After Luo Tingzhu asked, he didn't show Fang Ziye the information, but said: "The other party said that this is a new cell line constructed by their team, and if you take it wrong, you will take it wrong, and there are still many cell lines in their research group, so you don't need to return it." ”

"It's HuO9, how are you going to give it to me now. I can pick it up again, and the other party can come and deliver it. ”

Fang Ziye thought for a moment: "If she sends it, it will naturally be the best." ”

"The team that can build a new cell line should not be a small team, maybe the other party may really be a student from Xiangya Hospital affiliated to Central South University, but he accidentally took the wrong one."

"When cryopreservation, if the label falls off, it is possible to take it back by mistake."

Luo Tingzhu said: "Let her come here to send it on purpose, it's actually quite troublesome, I'll let her send it, I've met anyway, I know it's not a lie." ”

"We are also here to do scientific ......"

Luo Tingzhu then proceeded to edit the information.

The reason why you can't send them directly is because the cell experimental line and cell line can only be used for experiments and scientific research, and if you are not engaged in scientific research, it cannot be given to you.

Imagine if such tumor cells were put in the hands of ordinary people and thrown directly into the food, no one could be sure what would happen in the end.

And if you are exchanging with the other party, at least the other party has professional equipment, and the probability of determining that the other party is doing scientific research is very high, in this case, it is more reasonable to complete the mutual gift, which is more reasonable in the field of scientific research.

"This is the cell line of Ewing sarcoma, a cell line constructed by their team themselves, typical cells taken from the patient's body, and it has not yet been included in our country's national cell bank."

"I can't do experiments for the time being, but if I freeze it, it may be useful in the future." Luo Tingzhu also inquired about the source of the cell line.

"Then let's cryostore it first, our laboratory, it seems that there is no team doing Ewing sarcoma-related experiments at present!" After hearing this, Fang Ziye was overjoyed.

Sure enough, this must be a new thing, otherwise, just looking at the shape of the cell, you can increase so many knowledge points, then this is not scientific.

Good guy, being able to build a new cell line is also a bit overwhelming.

Fang Ziye has not yet understood how to construct a cell line, but he also knows that the procedure is cumbersome, and the steps and processes to be done are complicated.

But it doesn't matter, someone else has built it, and if it is recognized in the future, it can be used directly, which is equivalent to saving a lot of money.

Ewing sarcoma, Fang Ziye thinks that he may, he can change a small experimental direction and develop in the direction of Ewing sarcoma.

It's not that Fang Ziye is half-hearted, it's mainly that it has a lot of knowledge points!

Fang Ziye proposed to help with the cryopreservation of cells, but Luo Tingzhu naturally didn't agree, just said: "Senior brother, it's okay, I'll do it myself, and the operation process is not complicated." ”

"This is my senior sister, and she is your senior sister, called Xie Xin."

"She said she would send me a vial of the cultured HuO9 cell line as soon as possible."

"Senior Sister Xie Xin said that she was originally raising U2OS cell lines before, but the bottle of liquid nitrogen that she froze the U2OS cell line was empty for a National Day."

"She went home, thinking there was someone else in the lab, so she ......"

Fang Ziye remembered Luo Tingzhu's explanation, and felt pain for the other party's laboratory.

Lying in the tank, the liquid nitrogen tank has been empty for a while, and the cells in it have shed their skin if they don't die, how many cells have died at once?

Moreover, an empty liquid nitrogen tank represents the other party's liquid nitrogen tank may have two, or three, the size of this laboratory.

It is worthy of Xiangya Hospital, an old top university, with deep pockets, which cannot be compared.

"Well, it's good to confirm the identity of the other party, it's actually not bad to have one more senior sister who is traveling with you, and you can exchange cell lines with each other in the future."

"Or maybe buy a little bit." Fang Ziye praised Luo Tingzhu's good luck.

If you have such a connection, it is still within this major, as long as you get along well, Luo Tingzhu may have more cell resources and reserves in the future than herself.

Luo Tingzhu had already returned to the cell console, turned around and squinted at Fang Ziye and smiled.

Fang Ziye left the cell operation room with interest first.

Then he sent another message back to his teacher, and Fang Ziye began to work.

There are two main priorities.

The first is the revision and revision of the dissertation during the master's degree. The revision of the master's thesis, no matter how careful, cannot be overstated, it will accompany you for a lifetime.

If there is an obvious mistake, it can become a stain on yourself later.

The second is to face the upcoming doctoral re-examination, prepare the materials, and carefully see the re-examination process at the time.

Although it is said that the hospital has given a quota, the process still has to be followed, and try to go as well as possible to avoid the hospital having to quarrel with other re-examiners or something, which is not good.

Luo Tingzhu came out after doing the experiment, first stood next to Fang Ziye for a while, looked at Fang Ziye's revised paper, and did not interject, about ten minutes after reading it, she sat in front of her computer, and then began to knock and type.

The writing of her school-level project bid is also coming to an end, and in December, she has to submit it to the school to get money, which also has to be revised many times.

……

After Fang Ziye's experiment was over, the number of knowledge points obtained again was only eight.

Eight points of knowledge, although there are a lot, but compared with the previous more than fifty points of knowledge, Fang Ziye can't feel enough cool points.

"Tingzhu, can you help me review my master's thesis to see for typos or low-level logical errors." Fang Ziye sent out an invitation.

Such a graduation thesis has no gold content, mainly repeating the content of the basic paper he published in the first district.

Such depth and experimental flow, as a master's degree, is far enough, and doctoral graduation is no problem. But because the gold content is too high, it needs to be cautious, carefully corrected, and the content is also too much.

"Yes, senior brother." Luo Tingzhu nodded, and while exiting the automatic sensor door of the laboratory, she checked the keys, campus cards, mobile phones and other items in her chest bag, and only after they were neatly placed did she wait patiently for the elevator.

Fang Ziye waited until Luo Tingzhu finished his work before he said, "Well, thank you, after you check it, I have to ask my senior brothers to help check it a few more times, so I can rest assured." ”

"I heard that everyone in our session has to be sent to the blind review, so you can't be careless!"

Since the first year of Tianlin, it has become more and more difficult to graduate all master's and doctoral degrees. And the graduation of master's and doctor's degrees is also from a certain senior brother incident the year before last, and everyone must be sent for blind review.

Why, because a senior brother who graduated a few years ago, after graduation, when he conducted a spot check on his master's thesis, thundered......

It's getting harder!

"Senior brother, you must be fine."

Luo Tingzhu grabbed the strap of his backpack with both hands, his elbows were facing back, and the palms of his forearms were close to his waistline, although he could still see his slightly delicate figure through the tweed coat.

Luo Tingzhu smiled and said, "Senior brother, if your dissertation is given to me as a doctoral graduate, there is no problem at all, how can it not pass the blind review?" ”

(End of chapter)