Chapter Ninety-Nine: Delicate Thoughts (Asking for Subscription)
In the process of running the experimental process, Luo Tingzhu's mind was a little messy, and Luo Tingzhu felt that there was only one loss at most.
However, Luo Tingzhu hurriedly suppressed his mind and hurriedly continued to run the process.
After the experiment finally reached the stage where he could wait, Luo Tingzhu temporarily left the laboratory, and carefully labeled: "Please don't move, senior brothers and sisters!" ~LTZ”
It's a polite way to avoid thinking that no one is here to do it, or that you forgot to turn it off and accidentally throw it away.
This kind of thing has happened in the laboratory, so there is such a reminder.
Luo Tingzhu walked out of the laboratory, and when he walked in the direction of the small table and stool where he was, he happened to hear Fang Ziye's junior brother Jiehan annoying Fang Ziye: "Senior brother, why do you want to add this reagent to this step?" ”
"It's called dimethyl sulfoxide (DMSO), which is an important osmotic cytoprotectant."
"In order to prevent damage caused by the formation of liquid ice crystals, osmotic pressure changes, and disturbance of cell structure during cryopreservation of cells in liquid nitrogen at deep and low temperatures, it is necessary to use DMSO-containing cryoprotectants. DMSO can quickly penetrate the cell membrane into the cell, reduce the freezing point, delay the cryopreservation process, increase the intracellular ion concentration, reduce the formation of intracellular ice crystals, and thus reduce cell damage. Fang Ziye replied a little tirelessly.
"And what if you don't add it? What will happen? Or do you add other reagents? "Jiehan is like a curious baby.
Fang Ziye has not considered such a problem: "I don't know, at present, our country should not have made original progress and breakthroughs in cell cryopreservation technology. ”
"Jiehan, you used to be like this, you didn't read any books, did you just ask?" Fang Ziye asked more, after this Jiehan came to the laboratory, there were really a lot of questions.
"No, I always ask after reading the book, for example, DMSO, the molecular formula is C2H6OS, and it is a colorless and odorless transparent liquid at room temperature, which is a hygroscopic flammable liquid. It has the characteristics of high polarity, high boiling point, good thermal stability, aprotic, miscible with water, and can be soluble in most organic substances such as ethanol, propanol, benzene and chloroform, and is known as the "universal solvent......".
"It's just that I didn't find a protective effect on cells from its biochemical function, but I knew that this is a component of pesticides.
"According to the literature, DMSO is also a permeable protective agent, which can lower the freezing point of cells, reduce the formation of ice crystals, reduce the damage of free radicals to cells, and change the permeability of biofilms to electrolytes, drugs, poisons and metabolites."
"Then you say, can such a thing be applied to clinical practice?" Jiehan is a problem baby, and every question is very tricky.
"When you were an undergraduate, did you often ask questions like this?" Fang Ziye asked.
Jiehan thought for a while: "Well, many of our teachers are impatient with my questions, so they will occasionally reward me with some topics to do, thinking that I have a little talent." ”
Jiehan replied seriously, not thinking that it was a shameful thing at all.
I don't know how to ask.,I understand, I'll ask you a der?
But even Jiehan's problems are basically questions that ordinary people will not understand, and they are all very valuable research questions, and his scope of study is quite wide, thinking about problems from the perspective of biochemistry and traditional chemistry.
"Senior brother, ...... here"
Fang Ziye was drunk, but he didn't expect Jiehan to just do a cell experiment and copy the process of the cell experiment, as if he had 10,000 whys.
In the distance, Luo Tingzhu, who secretly glanced at Fang Ziye, couldn't help but crawl slightly, pursed his lips and held back a smile, ensuring that he was smiling toothlessly in his experiment book, drawing √ one by one, just to identify which step of his operation and experiment had been done and which step had not been done.
Immediately after, Luo Tingzhu's thoughts drifted a little far, mainly because she was thinking very formally about what Jiehan said when she was in the cell room just now, which made her mind slightly restless.
To tell the truth, in the five years since Luo Tingzhu entered the undergraduate program, the senior brothers who are good to her, and some relatively excellent senior brothers, have already become a little numb.
There are countless sisters, brothers, teachers, and classmates who praise her for being beautiful and good-looking.
The reason why she chose orthopedics, Luo Tingzhu felt that the senior brothers in orthopedics were better, and they had a certain sense of security here, and in addition, when she was interning before, she found that although orthopedics was the department of surgery, the incidence of medical disputes in the field was the lowest.
In addition, during her internship, some unpleasant events happened in the internal medicine department, and she resolutely chose surgery, after all, most departments of surgery will not choose to calm things down.
When Luo Tingzhu first came, she came to find a good teacher, five subspecialties, and she found that the competition in trauma surgery was relatively minimal, so she came to Professor Deng Yong.
The eight-year program directly selects the supervisor, as long as the supervisor agrees, there is no problem in admission, it does not occupy any place, and it is a free choice, generally speaking, there will be no doctoral supervisor who does not agree to accept eight-year students.
Even though the funds of Professor Deng Yong's group were stretched, Professor Deng Yong still accepted Luo Tingzhu as a student, and after coming here, Luo Tingzhu felt a sense of security that had not been seen for a long time.
Whether it was the previous senior brothers who were willing to stand up for her and stop the 'rich and powerful' people who annoyed her, or the senior brothers in the orthopedic department who were willing to give her more opportunities, Luo Tingzhu was quite moved.
Of course, this kind of touching is not only the existence of orthopedics, nor is it only Fang Ziye who is like this to her.
If you want to say that people who are good to her, even among the senior brothers in orthopedics, Fang Ziye is not as good as Senior Brother Sun Shaoqing, and even less than Senior Brother Gu Yi, who is in the second year of the doctorate, who takes good care of her.
However, there is only one special point in Fang Ziye, that is, Fang Ziye is the only one who has better talent than her and faster progress than her, whether it is all his peers Luo Tingzhu has met so far, or his senior brothers.
It is not only the experimental talent, but also the clinical professional operation ability.
So much so that at the beginning, Luo Tingzhu faced Fang Ziye as a senior sister many times in the practice room, but he just regarded himself as a junior sister, and took the trouble to point fingers at Fang Ziye.
Although she is called Senior Brother Fang Ziye.
But this kind of time didn't take long before Fang Ziye suddenly caught up, and then suddenly, suddenly, he ran to the front.
On the other hand, Luo Tingzhu also knew that he was in Professor Deng Yong's group, and he definitely didn't have enough experimental funds when he first came in, and he relied on the help of his senior brothers.
For example, Senior Brother Gu Yi, Senior Brother Wang Yuanqi, and even Senior Brother Yan Zhiming, who was admitted by Professor Deng Yong in advance, will give Luo Tingzhu some reagents, operating equipment or something.
At this time, Fang Ziye didn't say anything, and finally she begged Professor Deng Yong, hoping that Professor Deng Yong would talk to Teacher Yuan Weihong, because Teacher Yuan Weihong's research group still had some spare money.
She thought about doing the experiment with Fang Ziye, a senior brother who had a better operation and a relatively high success rate of basic cell experiments, so that she could be familiar with the process of basic cell experiments.
can get a certain amount of project funds for herself, and now the result is that Luo Tingzhu has succeeded, and she will feel that she is actually a bit powerful-
Although it is said that some key experiments were done by Fang Ziye for her, Fang Ziye was willing to help.
But in fact, these things can be done by other senior brothers, but the success rate is not as high as Fang Ziye.
If the opportunity is right, no one will mind studying with better people, finding better teachers, and finding a better job.
In order to save money and avoid wasting after failure, resulting in insufficient experiments in the early stage, and when applying for a project, the success rate decreased, she asked Fang Ziye to help a few more times, and then by the way, she also rubbed some of Fang Ziye's experimental processes to accumulate experience for herself, and only now, she can be more familiar with all the processes of basic cell experiments in just a few months.
Teacher Yuan Weihong did not say that he could use his project funds to do experiments, and Luo Tingzhu did not mention this matter at all! .
For her, parents are hard to find, let alone others?
Moving forward slowly, doing basic cell experiments is never achieved overnight.
Luo Tingzhu thought for a long time that he might be able to take the subject and be ahead of Fang Ziye, after all, his instructor was a high professor, so he could continue to maintain a little leading advantage.
But this time, although Luo Tingzhu won the school-level project, but in terms of the city-level project, the project she applied for did not win the bid, but Fang Ziye won the bid, which made Luo Tingzhu puzzled.
Around November, Luo Tingzhu felt that he might have met a 'master' of the same level as himself, Senior Brother Fang Ziye, who had been unknown before, was actually missing a 'trigger point', once it burst out, all the potential burst out.
At that time, Luo Tingzhu actually occasionally maintained his desire to fight with Fang Ziye.
Healthy competition and the improvement of mutual comparison ability is a good mentality.
At the beginning of December, after knowing that Fang Ziye's incision technique had reached the requirements of the practice room further than himself, Luo Tingzhu's first reaction was unbelievable, and his second reaction was that his talent was actually not at the same level as Fang Ziye, and Fang Ziye was too good.
Since then, Luo Tingzhu has done something that many people are not aware of, that is, she slowly pays attention to Fang Ziye's work and experimental status, including sometimes when she is in the practice room, she will quietly take a look at what Fang Ziye is doing.
The things she does are all the same as hers, decent.
But it was this kind of attention that made her extremely shocked, and finally compared the nicknames given to Fang Ziye by the old professors.
Backgammon, no need for this name.
For example, in the recent hemostasis technique, Luo Tingzhu watched Fang Ziye stop bleeding forty-three times, forty-four times, forty-seven times all the way to fifty-one times in one minute, and occasionally fifty-five times.
This time is only one month, and it will take at least two months or even longer for herself......
And such a long period of attention made Luo Tingzhu's mind a little confused occasionally, and even occasionally when he saw Fang Ziye, his mind was not so calm.
Then it was when Luo Tingzhu worked hard for a long time, and finally felt that he could compete with Fang Ziye in the incision and break the level, and he planned to perform silently.
Fang Ziye called someone over in advance, and her performance was really silent, but in the end, Fang Ziye found out that something was wrong with her incision practice materials, and asked people to call her back again, so that Professor Deng Yong could see her progress.
To get along with excellent people, you must release the mode of getting along with excellent people.
At the beginning, Luo Tingzhu regarded Fang Ziye as a junior and disciple, so he had the mentality of instructing, and later as a classmate, 'the same kind', so Luo Tingzhu would send his own bids and so on to Fang Ziye to revise, so that they could be regarded as mutual exchanges.
Privately, Luo Tingzhu has also done a lot of literature exploration on the frontier research and corresponding pathways of giant cell tumor of bone, including some literature reserves on the upstream and downstream pathways of HK2 that Fang Ziye is currently doing.
The plan is that if Fang Ziye comes to ask, she can give a gift in return at any time.
But Fang Ziye didn't send the bid over for a long time, and she thought that Fang Ziye couldn't apply for the subject by herself, so she didn't have such ideas and preparations.
That's okay, come back next year.
Next year's Fang Ziye will be able to apply for the project by himself.
Adhering to this mood, Luo Tingzhu suddenly found that Fang Ziye didn't want to let him help him at all, after learning from Professor Deng Yong that the municipal project was taken by Yuan Weihong, Professor Deng Yong didn't care at all, because he didn't like it, but Luo Tingzhu was another small break.
Does she think Fang Ziye can't look down on her ability?
However, it was not until later that Luo Tingzhu slowly abandoned such a unilateral idea, Fang Ziye did not look down on it, but Fang Ziye did not need to help, because Fang Ziye had already done the preliminary research to a deep ......
may not be operated properly, and the quality of his entire scientific research integrity will collapse.
Of course, scientific integrity here is not cheating, but asking for money.
Obviously you are almost done, and you still need money to do such a project, isn't this another kind of dishonesty?
Naturally, it is impossible for Luo Tingzhu to report such a thing, is there anything wrong with Fang Ziye's own savings from his previous project fund, the money he picked out, and his own experiments?
There's nothing wrong with that. Anyone in the laboratory knows a principle, that is, if you can save it, you will save it, and if you can't save it, you won't save it, but Fang Ziye's degree of slashing and saving is even higher than her.
This made Luo Tingzhu, who has always been careful about budgeting, even more curious......
I don't know how long it took, Fang Ziye suddenly said: "Xiao Jie, let's stop today's Q&A session here, your thinking is too out-of-the-box, your senior brother can't keep up." ”
Fang Ziye confessed and raised his voice slightly.
Jiehan's whole person was as usual, and the mole on the corner of his right eye flashed again: "Oh, good senior brother." ”
Such an answer is quite regrettable, and the meaning is still unfinished.
Fang Ziye asked again: "Do you usually ask questions like this with Lan Tianluo?" ”
"Well, Tianluo also has a lot of questions, and he even asked why the white blood cell count is to the sixth power, not the fifth power." Jiehan nodded.
Fang Ziye could only give a pair of thumbs up.
Then hurriedly opened away: "Listen to Zhu, is your experiment almost over?" Let's take a look? ”
Luo Tingzhu heard Fang Ziye ask, and his soul returned to his place again.
While nodding, he looked at the time: "Senior brother, it will take another hour." ”
"Oh." Fang Ziye felt very regretful, looked at the time again, and then looked at his junior brother Jiehan, and moved a position, he was really scared by Jiehan.
Through this, Fang Ziye couldn't help but suspect that the reason why Jiehan had not had a second outpatient visit with Yuan Weihong until now may be this reason.
Once, it was in August, and Jiehan had just entered school, and Fang Ziye felt that his junior brother had just come, so he gave Jiehan a chance to go to the clinic and asked Yuan Weihong to bring Jiehan with him, which was a courtesy.
Yuan Weihong also agreed, thinking that he should be nicer to the new apprentice, but since then, Yuan Weihong has never mentioned this matter.
Fang Ziye opened his laptop and began to draw again.
Moreover, Fang Ziye currently has a lot of pictures, which need to be classified, organized, and established folders one by one, including original pictures, original data, etc., all of which must be classified one by one.
There are a dozen small folders in a large folder, and seven or eight folders in a small folder, and then it comes to the data storage link.
It is equivalent to the result of hundreds of workloads, but basically, except for HK2 and giant cell tumor of bone have become a closed loop from the perspective of cell experiments, the others are still open-loop.
Fang Ziye is afraid that sometimes his brain is hot, in case when writing the bid, all the results are put on it, and the reviewer of the project will take a look, you TM is done, apply for a fart project fund support?
At this time, Luo Tingzhu, who was sitting in the distance, was also quietly improving the data presentation link of a meta-analysis he had newly written.
It's just that the speed of typing is very slow, and I think in my heart that every time Senior Brother Fang invites himself to do experiments and participate in experiments, he is very sincere, so he shouldn't despise his ability.
Maybe it's enthusiastic, after all, Senior Brother Fang is happy to participate in all the senior brothers' projects, as long as he has free time and senior brothers are doing experiments, he is willing to participate.
Don't be blind, do more, think more, think less, oh no, or don't think blindly.
Luo Tingzhu began to think about it again.
It wasn't until an hour later that Fang Ziye took the initiative to invite Luo Tingzhu to participate in the later experiments like a timed alarm clock.
Luo Tingzhu panicked a little, accidentally deleted a line of words, and hurriedly used the withdraw button to withdraw the sentence, and then said, "Senior brother, wait a minute, I'll finish writing this sentence." ”
After Luo listened to Zhuzhu, he wrote a few words in the hip replacement, looked left and right, and then clicked the save button of the file.
got up and began to tear a pair of experimental gloves again, and walked into the laboratory in parallel with Fang Ziye.
(End of chapter)