Li Yuanpei's figure is equal to Fang Ziye, but he is a little taller than Fang Ziye, his complexion is slightly darker, and his face is full of potholes, which seems to be full of stories.

Today's face is still mixed with a kind of haste and paleness.

Li Yuanpei is not a special name, nor does he have a special identity, but under his own efforts, he has added two SCI articles, and one is also in District 2, which is all Yuanpei's own efforts, without the teacher's feeding.

If Fang Ziye hadn't been because of the article sent by Yuan Weihong, in front of Li Yuanpei, even if it was a tool article made by Jiehan, it would be a younger brother.

Naturally, Li Yuanpei's basic article was helped by his senior brother. Where does Li Yuanpei have so much time to do experiments?

The Chinese Academy of Sciences divides the SCI division according to the three-year average impact factor of the journal, Zone 1: Top 5%; Zone 2: 6%~20%; Zone 3: 21%~50%; Zone 4: Bottom 50%.

The articles in Zone 2 are in the top 20% of the average impact factor of all SCI journals, which is global, attention, it is global!!

The basic articles of the second district are not something that can be obtained casually.

In fact, if we look at the number of journals in Zone 2 and the total number of manuscripts published in each journal in the past ten years, it may be less than 1% of all doctors in China combined.

In other words, 99% of doctors may not be able to publish a treatise-type article in Zone 2, which is not alarmist, nor is it deliberately elevating the weight and efforts of Li Yuanpei, but a person who can speak with facts.

However, Fang Ziye had no choice but to look at his brother Pei silently, and turned around and walked to his console with complicated thoughts and eyes.

Everyone's life history and life experience are their own. Li Yuanpei's master's supervisor is a doctoral supervisor, and he is Professor Deng Yong, so he has taken advantage of a lot of convenience!

"Are you wearing a little less today?" Fang Ziye and Luo Tingzhu walked in the direction of the laboratory side by side, and when they saw Luo Tingzhu rubbing their hands, they asked.

"One thing, the weather forecast when I go out is deceiving." Luo Tingzhu didn't shy away, but just quickened his pace. "Senior brother, let's go a little faster."

It doesn't matter if it's cold, just wait until you get inside the experimental building, where there is air conditioning.

Just like when I used to study, it doesn't matter if it's cold, after class, it's good to wear more tomorrow, after all, grandma was old at that time, and her legs and feet were inconvenient.

When it was cold in high school, I got into the quilt more, and I couldn't go back to bring more clothes until I had a monthly vacation......

Fang Ziye didn't pretend to take off his down jacket to Luo Tingzhu at this moment, pretending to be a gentleman.

It's really cold!

Close to East Lake, the wind blows so that there are no buildings that can stop it.

Soon, the two of them jumped up the steps of the laboratory building, and then Fang Ziye took two steps ahead and put Luo Tingzhu through the crack in the door.

Luo Tingzhu stomped his feet inside, and his lips were slightly pale.

"Isn't it uncomfortable? Why don't you go back first? Fang Ziye asked.

Luo Tingzhu swayed from side to side like a penguin: "No, senior brother, it's not cold inside the laboratory building." ”

Luo Tingzhu herself is not hypocritical, even if she is now in her menstrual period.

Fang Ziye didn't say much to press the upward button of the elevator, and said, "Senior sister, I will be semi-full-time next month, if you have any experimental aspects, you can throw them all to me." ”

"Good! ~" Luo Tingzhu then nodded......

In the research group, with new money, even if it hasn't come down directly, Fang Ziye and Luo Tingzhu both have money to do experiments.

In Yuan Weihong's youth fund, the money left for animal experiments can be saved for the time being, and the project funds in the hospital will be used to make up for it later.

I didn't dare to move before, because I was afraid that I would not get the money, and then when I settled at the end of the year, I had to settle the account with someone else's material company to avoid legal disputes.

But with money, it is definitely possible to take credit first.

As soon as she arrived in the laboratory, Luo Tingzhu opened her backpack and took out a notebook from it, which was very carefully planned, the tens of thousands of yuan she could use at present, and the general things to buy, were all recorded on it.

The same is true for Fang Ziye, the experiment is to be planned in advance, and the remaining experimental equipment of Yuan Weihong, such as the pipette head, has been exhausted and needs to be replenished, so Fang Ziye began to plan according to the corresponding template that Luo Tingzhu had prepared, and then submitted it to the material company, so that the company could deliver it to the door of the laboratory.

At this point, the services of many gene companies are in place. As long as the address is detailed enough, it can be reached on the same day, unless you need a lot of consumables.

It's just that Luo Tingzhu also asked: "Senior Brother Fang, are the consumables of Jikai Gene you bought expensive, the price of the company that Senior Brother Sun usually docks seems to be a bit high, I checked it on the Internet." ”

"I'll see." When Fang Ziye heard that he could save a little money, he was also interested.

If you have money to save, you can do more experiments, which is crucial.

Spend more when you should spend, and save what you should save.

Moreover, because it will take a month of semi-full-time production, Fang Ziye plans to put all the knowledge points in the past month on the experimental operation, and strive to add the basic experimental operation to level 4, so that in the next three months, he will be in a semi-full-time state, and Fang Ziye can do more experimental operations.

Further improve the success rate.

Level 3's proficiency in basic experimental operation techniques is comparable to that of an academic doctor, and Level 4 is estimated to be at least a senior associate researcher level, right?

After all, 500 points of knowledge.

Associate researcher is a research title, and after graduating from a doctorate, you can smoothly enter the trainee researcher, and even during the doctoral period, you can be rated as an assistant researcher.

The researcher title series includes research intern, assistant researcher, associate researcher, researcher.

More than 4 years as a research intern, or more than 2 years as a research intern after obtaining a master's degree, or obtaining a doctoral degree, and have a solid basic knowledge and professional knowledge of the discipline, and be able to carry out research work independently; You can get the title of assistant researcher!

If Fang Ziye is willing, he can consider evaluating the title of an assistant researcher next year.

"It seems to be a little cheaper, and the quantity is preferred, why don't we order it together, and then divide the account according to the quantity?" Fang Ziye asked.

Luo Tingzhu said: "Don't worry, I'll ask other internal medicine sisters to see if they have cheaper and acceptable quality consumables companies......

"Then I'll ask too." Fang Ziye thought about it for a while, and also planned to give full play to his connections.

After all, before the research funds in the hospital came down, the research funds in Yuan Weihong's research group were only about 200,000 yuan, which could not be used indiscriminately.

An animal experiment, whether or not about 200,000 yuan can be taken, Fang Ziye is not favorable for the time being.

……

Fang Ziye has done things with explanations, and the first one to confess is his next master, Professor Deng Yong.

Lest he be like last time, he was not in the department for a long time, and he didn't report to Professor Deng Yong, and he didn't talk about what he was going to do. Fang Ziye told Deng Yong!

Professor Deng Yong said, "I know, Professor Li has already contacted me. You also have to work hard to make more contributions to our team. ”

"Although I was removed from the position of ward director, it just allowed me to have more time to go forward and rush again. Let's see if there's a chance to make an 863 out this year......"

"Huh? Master. Fang Ziye was stunned.

"You don't care about this kind of thing, this is something that Associate Professor Hong Zili, and your master and I have to consider together. You're in the lab, half-time for months, okay, I remember. Professor Deng Yong seems to be full of energy.

Coming out of Professor Deng Yong's Zhenggao office, Fang Ziye's face was full of doubts.

The director's office is not exclusive to the ward director, and all the heights can be used to rest.

The second thing to explain is to give an explanation to Jin Hongzhou.

Jin Hongzhou was not found in the department, but Fang Ziye was in the emergency department, found him who was running an emergency consultation, and said the general situation.

Jin Hongzhou nodded: "Okay, don't worry, Viagra has already told me, it's okay, half-time is half-time, there is no shortage of bedside doctors in our ward." ”

Fang Ziye hurriedly explained: "Brother Zhou, I mean the matter of joint dislocation treatment in the department, you may need to connect with other senior brothers or junior brothers. ”

Jin Hongzhou suddenly realized and nodded: "Understood!" ”

"But I remember you." For Fang Ziye's sensibility, Jin Hongzhou really feels reliable.

Do things in a measured manner, come and go with explanations, try to ask such a subordinate, do you trust or don't trust?

After the handover was done in this way, Fang Ziye's main task plan for the first half of January came out.

First, the writing, verification and pre-defense of the graduation thesis.

Yuan Weihong arranged two pre-defense sessions for Fang Ziye, one invited Associate Professor Xie Jinyuan and Qin Geluo, and the other invited Professor Deng Yong and Associate Professor Hong Zili, as Fang Ziye's mock defense committee, to review and control Fang Ziye's defense process and defense content, which were arranged on January 8 and January 18 respectively.

However, Fang Ziye arranged three smaller pre-defense sessions for himself, on January 5, January 10 and January 15.

In this scene on the 5th, Fang Ziye just invited his own junior master, Lan Tianluo and Jiehan, to video himself and review the process of pre-defense.

Whether they are nervous, whether there are errors or omissions in expression, whether they are unfamiliar with the data in the paper or other aspects, the two of them will ask questions as part of the defense committee that they think are important.

On the 10th, Fang Ziye invited more people.

At this time, the advantages of connections are displayed, and among the entire masters, as long as the people who are not on duty, including Luo Tingzhu, except for Senior Brother Sun Shaoqing, are willing to appear and help correct one or two.

In this scene on the 15th, Fang Ziye did not invite his junior brothers and classmates, but invited Jin Hongzhou and Li Nuo as the main directors, and then informed his master, which was equivalent to forming a young defense team to ensure that nothing went wrong.

Of course, in addition to the online publicity for one month, there must also be a poster publicity.

At this point, Fang Ziye is very sure, it is Zhang Luo by Jiehan and Lan Tianluo, and they will work with Li Yuanpei to lead several other juniors and disciples to put the defense posters of Sun Shaoqing and Fang Ziye under the surgical building for a week, and Fang Ziye will also be relieved.

The second thing is naturally that Fang Ziye continued to experiment step by step.

Fang Ziye has short-term and long-term plans for the route of scientific research planning. The experimental content to be completed by Fang Ziye is the link in the recent planning.

It is based on the HK2 and giant cell tumor of bone that the teacher applied for from the National Natural Youth Science Foundation, but a lot of things will be added to it.

1. Metformin + HK2.

Its own concentration has an effect on the quantitative regulation of HK2 and further on the biological characteristics of giant cell tumor of bone.

3. After metformin was added to the medium, whether the expression level of HK2 and mRNA were affected, and compared with the standardized HK2 and mRNA levels.

4. Does the connection between glucose metabolism and HK2 in giant cell tumor of bone affect the subsequent pathways of glucose metabolism and the formation of products? What about metformin?

2. Longitudinal comparison with LDH pathway on glucose metabolism and biological characteristics of osteosarcoma.

This is that Fang Ziye is about to apply for a provincial-level project and all the experimental content to be completed.

Naturally, Fang Ziye also had to make some plans for Yuan Weihong's application for the next step of the formal general project fund.

Mainly in the HK2 pathway, siRNA is added.

As a new type of gene silencing technology, siRNA interference technology has become a key development direction in the field of drug research due to its advantages of high efficiency, high specificity and low toxicity.

In this way, the subject channel of Yuan Weihong's youth fund has been deepened by two layers, and there is preliminary research, and there is a lot of opportunity to apply for the general fund.

The third thing is that the meta-analysis that Fang Ziye is writing recently must be completed and submitted before the defense. And the priority of this step should be ranked first.

Because of the previous two things, Fang Ziye's preparation is already quite sufficient, which is equivalent to just taking the corresponding steps, but the meta-analysis article is a kind of examination of his thesis writing skills.

Although it is said that there are junior disciples who can help, but if you can not ask for help, you still have to temper your ability. It can be written by himself, let him revise and review, but he can't rely on others entirely, which is the basic quality of a scientific researcher.

Things are done one by one, and the meals must be eaten one bite at a time.

……

On January 5, after Fang Ziye's first pre-defense with the two juniors was completed, the two asked two more pointed questions, and Fang Ziye did not want to answer.

From the junior brother Jiehan: "This classmate, if you are told that after silencing the HK2 gene, normal people will die, then does your subject have any meaning?" ”

Fang Ziye actually had the answer in his heart.

Big brother, this is a basic experiment, an exploration, not a confirmation, not a real development of tumor targeted drugs, if you have to take all the situations into account, 99.95% of the world's projects can be stopped, do Nima, do it......

However, Lan Tianluo's question is more acute, but very sincere.

"This classmate, have you ever considered how many times the difference between the bypass pathway of glucose metabolism and the direct path of sugar metabolism is different utilization efficiency? Does this affect the biological properties of the tumor cells? ”

Fang Ziye also answered.

But the answer doesn't need to be judged by these two people, play a ball!

This Lan Tianluo is simply unsatisfactory, and I didn't talk about the bypass pathway of sugar metabolism, what are you doing with this?

HK2, giant cell tumor of bone, the orthodox pathway of glucose metabolism, you have to ask this.

But considering that Lan Tianluo was born in the Department of Mathematics and was more sensitive to data, Fang Ziye bypassed him.

After coming to the laboratory, Luo Tingzhu said: "Senior brother, I know a company here, and recently petri dishes are on sale, and the New Year's Day activities are five cents lower than the usual petri dishes, and two yuan lower than the culture flasks." ”

"Is the quality passable?" Fang Ziye asked.

The low price is a good thing, but the petri dish is originally two or three yuan, and the culture flask is a little more expensive, and there are more than ten or twenty yuan.

"Non-operational pollution is compensated, and damage is replaced. The main thing is to clear the inventory. ”

"How many are there?" Fang Ziye asked.

Generally, a pack is about 12, and a box is about 240.

"200 boxes!"

"I can't buy a lot of that, so I'll get six packs of petri dishes, and if you're looking for a culture flask, let's get a box."

"By the way, listen to Zhu, I will also switch to pipetting tips in the future, and I don't need a pipette." Fang Ziye touched his nose and explained.

"Yes, senior brother, pipettes are too expensive, one costs two or three yuan, and it is not cost-effective."

"When the amount of operation is large, it is still more cost-effective." Luo Tingzhu has always felt that Fang Ziye's team is more luxurious to do experiments, and it can still be more meticulous and budget-conscious.

"It would be nice if you could find a batch of reagents that can be cheap, but the cost of reagents is actually the big one." Fang Ziye sighed.

Luo Tingzhu said: "It's okay, senior brother, there are some reagents, in the relatively large laboratory of internal medicine, the wealth is rich, I can go and use the tube to divide some over." ”

"But the request is that we help them do some experiments."

"That's okay, some reagents are ridiculously expensive, we are equivalent to making extra money, we can do it."

"But take a closer look at the experimental process, don't run a particularly complicated flow formula, run hundreds of them at a time, then definitely don't do it." After Fang Ziye finished explaining, he turned on the computer and began to write articles.

Actually, I can write at home, but it's cold at home, and I have to turn on the stove or air conditioner.

The atmosphere in the laboratory is excellent, warm and comfortable, and you can go to see cells or do experiments at any time, which is very suitable for learning.

Luo Tingzhu put away his workbook notebook.

This notebook is a kind of important processing notes that she deliberately uses for non-experimental data recording.

"Senior brother, I'll go to the practice room first." Luo listened to Zhu's words, quietly stuffed Fang Ziye with a rice cake and two pieces of chocolate from the snack bag, and ran first.

Fang Ziye had already put in the work, so he didn't reply.

When Luo Tingzhu walked out, he kept thinking in his heart, while Senior Brother Fang was half-out of work, I must catch up with him......

(End of chapter)