Chapter 119: Treatment of the Little Boss (Asking for a Subscription, Asking for a Monthly Pass)

"Senior Brother Qian, did you order takeout at night? Why don't you order it together? Although Fang Ziye's current deposit is not much, he still generously invites.

In January, Fang Ziye had both merit and hard work, so Master Yuan Weihong rewarded 2,000 oceans of labor expenses, which was the capital of funds.

At the same time, the big boss, Professor Deng Yong, also generously gave 2,500 oceans, and gave Fang Ziye half of his blood back.

It's time to give it!

Not to mention that Fang Ziye ruthlessly gave face to the orthopedic department of Zhongnan Hospital in the re-examination stage, even if Fang Ziye took a municipal-level project, Yuan Weihong should give a small reward appropriately.

Professor Deng Yong was not reluctant, but went to his private account.

Fang Ziye is not a student of his name at present, at least in the school system, so he can't go through the public account and give some rewards from the fund book.

However, Professor Deng Yong still gave special approval, if Fang Ziye posted an article in the future, the hospital gave a bonus for redeeming the prize, and Fang Ziye could get a large part of it.

Naturally, this thing, as Fang Ziye's mentor, Professor Deng Yong still has to get it, otherwise others will think that there is no one under him to help do things, and the teacher is not in harmony with......

"Yes, Ziye."

"What do you want to eat, I'll help you order it with Senior Sister Tingzhu." After Qian Qiaofeng was quite exquisite, he explained: "After all, your senior brother's monthly subsidy is several times that of yours. ”

At the doctoral level, the monthly subsidy can reach the level of one-third to one-half of the average income of the city. It is only a little less than an ordinary resident like Lan Tianluo, which can be seen to be very impressive.

"Then thank you, senior brother, I think the barbecue rice or a little rice bowl will do."

"Senior brother, you are looking for me, there should be something, right?"

"If the junior brother can help, he will definitely help." Fang Ziye mentioned the key points first, after all, Fang Ziye is here, there are still a lot of things.

Qian Qiaofeng asked while swiping the interface of the takeaway: "Ziye, do you know how to operate molecular probe technology?" ”

Qian Qiaofeng asked casually, but he was actually testing Fang Ziye's technical operation level.

Cell experiments are also horizontally differentiated.

Generally speaking, macromolecules such as HK2 are mostly in the cytoplasm, and even mRNA is also in the cytoplasm.

Cells are divided into cytoplasm, nucleus and organelles, and the structure of different operating targets is completely different.

miRNA, on the other hand, regulates the transcriptional formation of mRNA and is at the organelle interface. But the molecular probe technology is the real technology, the nuclear level technology.

At present, in China, the treatment of cell nuclei mostly relies on reagents, plasmids, etc., which directly cause violent reactions, without paying attention to clarifying the corresponding principles very thoroughly.

But in order to operate molecular probe technology, then you must be very clear about these principles, and to a certain extent, make your own single-stranded DNA molecules, which can be cloned or PCR amplified, or artificially synthesized oligonucleotides or RNA molecules transcribed in vitro!

The probe must be pure and contain no other different nucleic acids. In order to ensure that the gene of interest is detected by base complementation!

To put it more generally, the current domestic genetic testing technology relies on instruments, not operations, but high-precision cell experiments can process and even test the molecular structure of nucleic acids without special instruments.

This is molecular probe technology.

Fang Ziye's eyes flickered many times in an instant: "Senior brother, have your cell experiments achieved the level of directly processing the nucleus?" ”

Fang Ziye was very smacked.

Without him, Yuan Weihong's current depth of cell-based experiments, that is, at the level of HK2, has penetrated it to the mRNA and miRNA levels, but this still belongs to organelles.

The deeper molecular probe is a level deeper than the hnRNA that Fang Ziye will carry out next!

The more in-depth the research, the more thorough the understanding and control of the collation, which is a major trend of development.

It can be seen from this that there is a big gap between his small research group and the big research group of Hong Zili, and even the team led by Professor Deng Yong can't keep up at all.

The level involved by Senior Brother Yan Zhiming and others is at most on the basis of mRNA, plus a miRNA!

The simple direct molecule HK2, mRNA, miRNA, hnRNA and the gene sequences in the nucleus are in-depth and iterative, and the difficulty of operation is naturally exponentially improved.

"No, no, brother, you misunderstood."

"It's just that Master has the heart to rush in this direction, didn't my master see your operation today, so let me ask in passing."

"Ziye, it's not that Senior Brother said, you are hiding too deeply. If it weren't for watching you grow step by step, I'd love to cut you off the spot. ”

Qian Qiaofeng ordered three takeaways, put the mobile phone in the side pocket of the white coat, and joked: "Neck, to the upper abdomen, the direction of thoracic surgery belongs to me, and the direction of cardiac surgery belongs to Lao He." ”

"The four legs and the spine can be handled by Gu Yi and Yuan Qi, as well as the anus and ......"

Fang Ziye clenched his fists and said, "Brother, brother, don't say it." ”

"Even though we're both medical students, it sounds oozing."

"The little brother is willing to do whatever he wants."

Don't think that Qian Qiaofeng is joking, the senior brothers who often practice specialized anatomy in the practice room, if they come to the real thing, they can really dissect Fang Ziye clearly, and then go in and step on the sewing machine.

"Just kidding, don't blame me for being a straight man."

"I'll just say it."

"What my master means is that I will bring some money and follow you to do experiments, do a little ruthless preliminary research, and strive to engage in a relatively large national key project in the next step."

"Originally, Master meant to take the money and hand it over to the company to go to Australia and run out of these sequences."

"But there is a request for others, but there is no need for a brother. Right. Qian Qiaofeng was quite vague, and after beckoning Fang Ziye to sit down, he subconsciously wanted to light a cigarette.

But after thinking that Fang Ziye didn't smoke, he wanted to put it back silently.

"Senior brother, it's okay, you smoke, I personally don't mind." Fang Ziye greeted.

Then I thought, "Senior brother, is this really what Teacher Hong meant?" Say something you don't like to hear, in fact, I'm so jealous of you, senior brother, just spend the money. ”

If Hong Zili is willing to bring capital into the group, Fang Ziye will not mind signing a deed of sale to work for others.

There is no way, Fang Ziye has added all the knowledge points he has earned to the basic cell experiment in the past two days, and the current level of Fang Ziye's basic cell experiment is already as high as level 4!

Fang Ziye had no way of knowing how powerful he was, but when he read the literature, Fang Ziye felt that he could handle it to the level of the nucleus, but he didn't have the opportunity to operate.

Now if there is an opportunity, Fang Ziye can go and talk to Professor Deng Yong and his master Yuan Weihong about working for Hong Zili.

As soon as you have the money to do experiments for yourself, you have a lot of knowledge points.

Second, he has his own achievements on display, experiments he has done, and Professor Deng Yong endorses Fang Ziye, Hong Zili does not give Fang Ziye a total work, and he can't get out of this laboratory, which is ironclad.

Fang Ziye's teacher is the confidence that Fang Ziye feels can add chips.

"If that's the case, I'll say yes."

"But don't worry, junior brother, my teacher will definitely not treat you badly. I'll also take two junior disciples and Ziye to do experiments together, and we'll all make progress together, right? ”

"Ziye, if you are free, send your bank card number and ID number." Qian Qiaofeng handed Fang Ziye a very direct expression that you understand.

"Senior brother, let's not send this. It would be unreasonable to make a deal. "Fang Ziye loves money and loves money very much, but a gentleman loves money and takes it in a good way.

"It's not just giving you money, what are you panicking about. It's the hospital that gives bonuses and gives bonuses to the team. Qian Qiaofeng took a sip and said unhurriedly.

Fang Ziye's eyes widened slightly.

Well, it's because of his narrow-mindedness, Fang Ziye has never heard of the fact that the hospital will give bonuses to the team.

It may be that his two teachers do not have such a high height as Associate Professor Hong Zili in terms of research, so they can't get access to this kind of bonus, and naturally they don't have the opportunity to get a piece of the pie.

"Senior brother, then when will we start? Also, if you want to do molecular probe technology, the equipment in our laboratory has no foundation. Fang Ziye now has a lot of treasures for the equipment in the laboratory.

"You don't have to worry about this, my master has already applied with the hospital, and it is estimated that it will be approved soon, as long as the money is approved and stamped, the instrument will naturally be sent by the company."

"But Ziye, I'm going to mention it to you a little here, I will go abroad next month, about half a year, so in the future, it is estimated that you will take my junior disciples to do experiments together, and if you have some small operations, you can also rest assured that they will be handed over."

"The people I brought over, except for Zhao Yanlin, the other two people are all proficient."

Fang Ziye said: "Senior brother, you are polite, where will I bear this heart?" ”

"But I'll still ask the boss what he means." Fang Ziye didn't directly agree.

……

After chatting with Qian Qiaofeng for a while, Fang Ziye had the heart to sigh at the depth of the experimental progress of other research groups, while his own small research group could only climb forward step by step.

After Luo Tingzhu came to find him with the timer, Fang Ziye took his leave first.

Luo Tingzhu didn't ask what Fang Ziye and Qian Qiaofeng discussed, but Fang Ziye still mentioned this matter while operating with Luo Tingzhu.

After listening to this, Luo Tingzhu's whole body stiffened slightly: "Senior brother, can you even do molecular probe technology?" ”

This was Luo Tingzhu's first reaction.

Molecular probe technology, in the laboratory, can be sequencing freely on its own, which can generally only be done by foreign laboratories, and 99.99% of domestic laboratories are sent to biological companies for help.

One is the lack of equipment, and the other is that the technical barriers have not been breached.

Moreover, Luo Tingzhu quickly asked the second question: "Then according to Professor Hong's meaning, senior brother, you will be the little boss of our laboratory in the future?" ”

In the laboratory, there are big bosses and small bosses.

On the surface, Deng Yong is the big boss, the person in charge of the laboratory, and the main management. Then Associate Professor Hong Zili, who is the small boss, holds most of the financial power.

Of course, in fact, Associate Professor Hong Zili and Professor Deng Yong are separate states, and they are only doing experiments in the same laboratory, and they cannot absolutely distinguish between big and small bosses.

And Xie Jinyuan, Yuan Weihong, and Qin Geluo, who is about to be promoted to a master's tutor, and Deng Yong's relationship is a standard boss.

Fang Ziye is a student of Yuan Weihong, and Yuan Weihong is Fang Ziye's direct boss, giving money for experiments.

However, Professor Deng Yong is the big boss.

Generally speaking, those who can be called bosses are those who have money and can get the project. And the subject you get can't be a small topic, but a great person who can help others graduate and complete the work accumulation, so you can be called a boss.

For example, Fang Ziye can't be called a boss when he applies for a municipal project, but if he gets a provincial project, he can support an academic master's student to complete the graduation requirements, and he can barely be called a little boss.

"Don't use such a title, at least my masters are very good to me." Fang Ziye knows the origin of the title boss, and even many people call their teachers bosses.

But here in Fang Ziye, Fang Ziye has always shouted Master in a proper manner.

There are definitely rumors on the rivers and lakes and in the media, and they are the norm. Exploitation, bullying, and so on, and so on.

But Fang Ziye himself was glad that he would naturally not become the kind of senior brother or master as rumored.

"That's with the group." Luo Tingzhu changed his name.

Fang Ziye said a little stinkyly: "Listen to Zhu, aren't I just leading a small group now?" ”

Luo Tingzhu thought about it and nodded.

Fang Ziye said again: "Just kidding, taking a junior brother can be called a group, in front of you, junior sister, I don't dare to say that, you are holding such a large sum of money, and you are coming by yourself." ”

Fang Ziye grabbed it with his hands first, which means making money.

"I'm asking for this." Fang Ziye held it in both hands, making a begging motion.

It's not self-deprecation, that's what it is.

What you earn is the funding you get from above.

If you want to come, isn't it equivalent to begging?

Yuan Weihong gave it, and then Professor Deng Yong gave it, and what topic Fang Ziye applied for, it was also approved in the name of Yuan Weihong.

Luo Tingzhu shook his head and snorted softly: "Mm-hmm." Wrong. ”

"Senior Brother Fang, you are too self-deprecating, in fact, everyone in the group understands that there is no need to be so modest."

Luo Tingzhu took two steps back, gave Fang Ziye enough space to operate, and made an arch gesture on the operating table with his hands, and then explained: "In the past, my classmates said that I came to the trauma surgery department of the orthopedic department, which was absolutely condescending and wasteful. ”

"But now I feel like I'm making a lot of money."

"Even Senior Sister Yan Yu from the Department of Rheumatology and Immunology, and Professor Lin from the Department of Cardiology, can't do it, Senior Brother Fang, you can do it, who in the hospital can think of it......"

"You!" Fang Ziye leaned sideways slightly.

"Aren't you just watching?"

Fang Ziye was a little self-satisfied, and then admonished: "Don't talk about this matter first, when the results come out, you don't need to dictate." Otherwise, there is no real result, and it is all lip service. ”

"There's no need to roast our research group on a fire rack."

After returning from Qian Qiaofeng, Fang Ziye knew the current situation of Professor Deng Yong's research group, so he needed to be more cautious.

Being a low-key person and doing things in a high-profile manner is the only choice.

Luo Tingzhu bowed his head slightly embarrassedly: "Senior brother, I'm not a big-mouthed gossip. ”

Luo Tingzhu seemed to explain something, but Fang Ziye didn't ask the waiter at all, Fang Ziye could understand it!

Continue the experiment.

In the evening, at 8:49, when Fang Ziye ran out of the last set of WB photos, he was very happy.

"+0.2 Knowledge!"

"+0.3 Knowledge!"

"+0.2 Knowledge!"

"+0.2 Knowledge!"

“……”

"Current Balance of Knowledge Points: 169 points."

Do you dare to believe that Fang Ziye's income from a single day of knowledge points has reached 169 points through experimental operations and observing the results of experimental operations today. This corresponds to nearly one-third of proficiency in level 3 to 4 skills.

Fang Ziye himself couldn't believe it.

Even after gaining knowledge, Fang Ziye still took photos carefully and stored them in his mobile phone.

In case you watch the results again at a certain moment, you can increase it by 0.1, which is equivalent to an hour of concentrated practice in the practice room.

Luo Tingzhu watched Fang Ziye take photos from all angles, and his mood was slightly cold.

She seemed to know Senior Brother Fang Ziye more objectively, and she was more determined about the guesses in her heart.

But he doesn't seem to feel disgusted, because Fang Ziye's collecting habits don't seem to be the same as other people's collecting habits.

However, Luo Tingzhu searched a lot of information, but he didn't find the diagnosis of the subtype of academic collectivism, perhaps, Senior Brother Fang Ziye is a special collector's habit.

Aren't geniuses generally with their own personalities and tempers?

Luo Tingzhu thought so secretly, because she also has some quirks, which is to eat snacks......

"Tingzhu, you go back to rest first, and I'll talk to Senior Brother Qian and them again!" After Fang Ziye finished the experiment and cleaned up the operating table, he told Luo Tingzhu like this.

"Are you talking about photos?" Luo Tingzhu is already fully armed-

Backpack and snack backpack, plus holding a water cup in your hand, wearing fluffy rabbit ears thunderbolt gloves, looks a little silly.

Fang Ziye noticed that Luo Tingzhu's expression was not natural, but he still nodded embarrassedly.

"Almost!"

"You go back first." Fang Ziye naturally agreed to Qian Qiaofeng, and everyone cooperated to do the experiment together, so the form of wool that he could gather had to be full.

As long as there is a certain degree of participation, you can get knowledge points, even if it will be halved or even only one-tenth will remain, which is enough for Fang Ziye to work hard.

Who would dislike having less knowledge?

When Fang Ziye and Qian Qiaofeng made this request, although Qian Qiaofeng also felt strange, he also agreed very boldly, Fang Ziye's little habit is not a secret in the laboratory.

However, Qian Qiaofeng still believes in Fang Ziye's professional ethics and will not leak, sell or even steal these pictures.

After all, Fang Ziye can run the monk, but he can't run the temple!

It's just that Fang Ziye came out of the laboratory, took his mobile phone and looked at one of the pictures sent by Luo Tingzhu, and he couldn't help it!

replied with a voice: "Why are you listening to bamboo?" How do you take your own photos and still take other people's? ”

"What does it mean to be wearing stockings on such a cold day?"

"Can you not be so tempting, do you have any questions, you just ask directly?" Fang Ziye really couldn't stand it.

Usually Luo Tingzhu sent a selfie of herself, sandwiched in the middle, even if it was forgotten, this would send a black silk photo of her roommate, which was obviously a bit of a misunderstanding......

(End of chapter)