Animal Basic Testing Technique Package Level 5 (0/500000).

The color is slightly different from the previous fluorescent green, and it has become a pale gold.

Although the changes were not too dazzling and dazzling, Fang Ziye, as a skill owner, knew that he had now mastered a lot of content related to basic experiments with animals.

The basic experimental technology of animals is not necessarily the foundation, but the basic methodology, which is the basis of the operating object, rather than the operation technology is very basic and can be done by individuals.

Skills are the mastery of methodology, but mastering methodology does not mean that you are completely and thoroughly proficient in all animal experiments, and you still have to rely on the most basic principles and ideas to complete the design of animal experiments.

The establishment of animal models is just one of many animal experiments.

A long time ago, Fang Ziye knew that in order to establish animal models, the methodology used is actually called gene editing.

Gene editing technology is very simple when it comes to noun explanation!

That is, the process of modifying a specific target in the genome of an organism. Efficiently and accurately insert, delete or replace genes to alter their genetic information and phenotypic characteristics.

But it is not easy to really operate.

After all, gene editing technology, first of all, can be divided into gene knockout and gene knock-in in the general direction, which is actually similar to silencing and overexpression of a certain gene in cell experiments.

The basic principle is very simple......

Fang Ziye didn't think much about it, but was thinking about how to use this operation technology to complete the operation of his current self and his experimental direction, whether it would bring relatively large benefits.

The answer is obvious!

The design of the experimental direction should precede the completion of the operation of the experimental direction.

One is theoretical cognition, and the other is operational arrival.

It is equivalent to the pre-design that you know that a place exists, and the completion of the experiment is that you go to the place you want to go, which are two different concepts.

In fact, the design of the experimental direction does not require you to have super high operation ability, but your ability to collect and summarize data, and then make the general process.

As for whether it can be completed, it is related to personal ability.

Anyone who has been in contact with scientific research knows that everyone can imagine a technological direction that the world will feel shocked, but whether this imagination can be completed is another matter.

For example, the ladder to the moon, the Great Wall tiles, the Pacific swimming pool, and so on.

Fang Ziye has long been designing experimental directions, one of which is the establishment of the HK2 animal gene knockout model.

Looking through the notebook again and slowly reading the experimental design at that time, it seemed much calmer.

Now Fang Ziye no longer has the idea of being particularly excited and excited, on the contrary, after the improvement of this technical skill, Fang Ziye's mood and thinking have become a little calmer.

Fang Ziye doesn't know why!

Obviously, a few minutes ago, I really wanted to complete this project and then look at the face of that uncle, but after the experimental technology was added, this mood was not so sufficient.

Fang Ziye guessed, maybe, compared to making that master powerless in front of the master, his own ability improvement and the differentiation of his strength made Fang Ziye have a sense of achievement.

Rather than looking at some people's faces, what Fang Ziye hopes to do is to truly experience the happiness of experimental results.

Notes written with a pen, the ink is dry—

Experimental Design and Planning:

Identify the scientific objectives and questions of the study. Develop an experimental plan, including the selection of animal models, methods of gene knockout, etc.

Selection of animal models:

The selection of the appropriate experimental animal, generally mouse or rat, depends on the research question and the feasibility of the model.

These two points are completely excerpted from the basic theoretical part, which belongs to the general outline of the subject experiment, and has no special significance.

What's more important is below.

How to determine the specific method of HK2 gene knockout!

Common ones include CRISPR/Cas9 technology, embryonic stem cells from knockout mice, etc.

With just one sentence, the difficulty suddenly went up.

CRISPR/Cas9 technology is a kind of gene editing technology, editing genes, you need to rely on this technology, but how to knock out, and after knockout, to get the embryonic stem cells of genetic mice, suddenly becomes blurred.

The fact that the experimental animal cannot reproduce after the animal test does not mean that the experimental animal model is a complete body from the beginning.

This requires embryonic stem cells after fertilization of mice, where they have to be removed and gene-edited.

Then the steps are:

Design gene editing tools and in vitro gene editing experiments:

Gene editing of embryonic stem cells or other appropriate cells in vitro to verify the effectiveness of the editing tools.

gene editing in vivo assays:

Guided edited embryonic stem cells or gene-editing tools into animals, usually mouse or rat embryos.

Screening for normal gene edits:

Through molecular biology techniques, such as PCR, Southern blotting, etc., animals with successful knockout of the HK2 gene were screened.

Animal Reproduction:

Whole knockout animals are bred from successfully edited embryos, usually by breeding and selecting offspring that produce knockout bodies.

Verify gene knockout effect:

Detailed molecular biology and biochemical analysis of the resulting gene knockout bodies was performed to confirm whether the HK2 gene was successfully knocked out.

These seemingly impossible, particularly difficult experimental operations, when Fang Ziye reviews them one by one now, feels that the difficulty is not particularly high.

At least, it doesn't look like I looked at them before, I think it's just a castle in the air, like a dream bubble, and it seems a little more substantial.

This is Fang Ziye's own ability, which has obtained feedback on the feasibility of experimental operation.

Now that you've thought about it, let's do it.

For example, contact a large animal experiment company and ask them if they can provide embryonic stem cell extraction technology, and if not, whether they can provide the platform and address for experimental operation, and operate it yourself.

If so, how they transship, where to transit, these are the questions.

This is not an ordinary animal experiment, find an animal laboratory and do it directly, Fang Ziye suspects that he is very likely to have to return to Han City, or even Flying Demon Capital and Kyoto, or Flying Sheep City, such an economically developed, and medical technology level and located at the forefront of domestic scientific research.

But no matter what, everything is difficult at the beginning, and after you have an idea, at least you have the confidence to do it.

Fang Ziye contacted the large animal testing center in Han City, which his master Yuan Weihong had taken him to before.

The staff immediately asked about it.

A few minutes later, the person named Xiaoya contacted and said, "Teacher Fang, is the question you asked too high-end?" Our technical director, that is, the doctor of our company, said, "Your experimental direction is not something that our laboratory can operate?" ”

"Teacher Fang, we have other ...... here"

Fang Ziye looked at it halfway, and politely rejected her follow-up proposal.

If it's not difficult at all, why should I ask you, can't I just come over and do it?

Then I consulted with representatives of several other experimental centers, and finally found that there is only one animal laboratory in Han City that can operate in this way, and it is also in the internal laboratory of Tongji Hospital affiliated to Huazhong University of Science and Technology.

"Teacher Fang, the animal laboratory team of Tongji Hospital, in principle, only accepts high-level animal experiments from the staff of this hospital, and is not open to the public."

"Of course, if you need it, Teacher Fang, we can help you try to contact it." A manager of a biological company named Xiaohui replied so enthusiastically.

Fang Ziye's performance in this direction, if it can be won, at least it can be compared to their team, and it may be a year's performance.

If you want to build new animal models, up to tens of millions, less than two million, you don't have to try anything at all.

"Well, I'm just consulting first, thank you, Mr. Wang. We can get in touch if needed. Fang Ziye said.

He only needs to ask for the address first, and as for whether he needs to go through the teachers of this institute or through other channels, that is two words.

If there is really no way, let the other party help, this is the right reason, otherwise he will be stuck in the neck, and when the time comes, you will owe favors, you don't buy the other party's things, how inappropriate is that?

But what if the other company's stuff doesn't fit?

Tongji Hospital!

If it was placed in Xia En City before, Fang Ziye could only ask his teacher for help and ask him if he had a channel, but now, Fang Ziye could ask Senior Brother Wu Xuanqi.

In addition, Fang Ziye also knows that there are at least three experimental centers in Modu that can complete such operations, but at present, there are very few teams that will want to operate such high-end and cutting-edge animal test operations with high uncertainty.

……

Following.

After Fang Ziye finished checking the room, he chatted casually with Wu Xuanqi.

Wu Xuanqi's first reply was: "......"

Then the reply was: "Ziye, can we not hit people so badly, you are asking about gene editing technology, you are playing a bit high-end?" ”

"Do you have any good topics?"

"Well, senior brother, I also plan to try animal experiments to establish animal models, make some money, and explore a way." Fang Ziye was also unsure of his reply.

In the province and in China, there are not 1,000 but 800 teams that want to do ultra-high-end experiments.

Who hasn't had a dream?

But how many teams made it in the end? Most of the operations of most teams are to sink into the sand, which is equivalent to taking money to fetch water and feed the fish, but there are still and will be countless teams who will take money to sprinkle into the water.

"Ziye, let's be honest, are you sincere, or do you just want to play?"

"If it's sincere, we'll follow the process of sincerity, and if we just want to play, let's follow the process of playing." Wu Xuanqi was very enthusiastic.

"Senior brother, is there any difference?" Fang Ziye asked.

(End of chapter)