Sun Lien's theory was very convincing, and the other doctors in the treatment group were surprised to find after thinking about it carefully...... Sun Lien's sudden statement is still very credible.

The insufficient expression of AQP4 protein perfectly explained Tang Min's symptoms, and it was also fully in line with the diagnostic aesthetics of "single etiology". Coupled with Sun Lien's explanation that the insufficient expression of AQP4 protein seems to be the only hypothesis that can cover all doubts.

But...... This is still a hypothesis.

"I've never heard of this kind of genetic disease...... "Zhou Ce seems to position himself today as a character who pours cold water. He shook his head and asked Brune and Yuan Ping'an, "Have you heard of it?"

"Nope. Zhou Ce's question was answered in the negative. Neither Dr. Brunn nor Yuan Ping'an had ever heard of the lack of expression of AQP4 protein that could directly cause disease. And Xu Yourong said, "I even think ...... This may be a newly discovered disease. ”

"Isn't it?" Sun Lien felt a little flustered for no reason, he quickly took out his mobile phone, opened PUBMED and began to search for related reports, and only after a long time did he raise his head and said with a weak face, "UH...... It seems that there is none. ”

"There are no reports in the Chinese literature. Yuan Ping'an and Sun Lien came to a conclusion almost at the same time. He glanced at Sun Lien with some surprise, and then sighed sincerely, "It's really you......"

"You're certainly not complimenting me on my ability to diagnose. In response to this exclamation, Sun Lien gave an unceremonious white-eyed counterattack. Then he refocused on Tang Min's condition, "Case report and the like can be put back for a while, we still have to find a way to confirm the diagnosis and find a feasible treatment plan first." ”

"Confirm the diagnosis...... This one is difficult. Xu Yourong thought for a moment, then shook his head, "You can't biopsy the edema area again, and then check the AQP4 mRNA concentration in the sample, right?"

"If she does have underexpression of the AQP4 protein, the biopsy will cause her too much damage. The biopsy proposal was rejected by Sun Lien almost as soon as it was proposed. The previous two biopsies had caused almost irreparable damage to Tang Min, and now it was difficult to have a more likely diagnostic direction, but the plan to confirm the diagnosis was to biopsy again knowing that the sequelae of the biopsy were relatively large...... It's a bit of a stretch.

"I'll check the information again. When everyone was at a loss, Yuan Ping'an took the initiative to stand up. "AQP protein is a Nobel Prize-level discovery, and there will definitely be no less related studies in the future. I'm good at checking information. ”

"When you look up the information...... It's a good idea to see if there are any treatments that might work. Since Yuan Ping'an volunteered to check the information, Sun Lien naturally put another part of the matter that may need to check the information to find a way on his head. "The thing about the inspection...... I'll figure it out too. ”

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Of course, Sun Lien is not a god, and in this case, if you want to directly confirm that Tang Min's central nervous system has insufficient AQP4 protein expression, it can only be determined by biopsy. Trying to bypass a biopsy and get conclusive evidence right away is a completely impossible job.

But...... Just because there's no direct solution doesn't mean there's no way around. Sun Lien confidently took out his mobile phone, and then dialed a number to go out.

"Teacher Sun. The phone rang three times, and a woman's voice quickly rang on the other end of the phone, "Hello." ”

"Hello Qiangong. Sun Lien's tone of voice was extremely polite, and he first explained the reason for his sudden call, "Qian Gong, I want to ask, how long does it take to determine the genes on a single sequence?"

"Are you saying you want to stop the full-sequence sequencing that is going on now?" Ms. Qian, who answered the phone, was an employee of the company that Sun Lien commissioned to carry out the full-sequence sequencing of the genome. She was a little embarrassed, "The whole sequence sequencing has begun, this ...... It's hard to stop. ”

"The original full-sequence sequencing continues. I want to ask, is the sample you have retained here enough to do a specific sequence sequencing again?" Sun Lien estimated that the other party might feel that he was going to stop the test and not pay for it, so he quickly explained, "I have a new testing demand here." ”

"Well, if you want to test a specific sequence...... The sample is sufficient. Ms. Qian is also a smart person, and she quickly understood what Sun Lien meant. In practice, this requirement is even simpler - there is no need to even take any more samples, and the original whole genome sequencing is the result of one by one. If there is a need on Sun Lien's side, they only need to adjust the order of testing.

"Then help me test ...... first" The human gene for AQP4 is mapped to the junction between autosomes 18q11.2 and q12.1 and consists of four exons encoding amino acid sequences 127, 55, 27, and 92, with three introns of 0.8, 0.3, and 5.2 PB in length, respectively. If Tang Min's AQP4 protein is underexpressed, then there will definitely be abnormalities in these four amino acid sequences. Sun Lien stumbled over the unit he needed to test and asked, "How long will it take for the results to come out?"

"If you are in a hurry, Mr. Sun...... Ms. Qian communicated with her colleagues a little, and gave a minimum time limit for them to do, "The results will be available before the end of work today." However, if the official paper report is to be issued, it will have to wait until tomorrow morning. ”

"No problem. Sun Lien was overjoyed, "Then please." ”

Now, except for those giant-level bioengineering companies, most of the sequencing companies are having a hard time. The largest demand for gene sequencing services is mainly from universities and research institutes. And most of these places are able to do sequencing on their own – but they are probably not experienced graduate students.

But the biggest advantage of letting graduate students do sequencing is that it's cheaper -- isn't graduate students free labor for professors and associate professors? If the sequencing results required by the project are not too urgent, and the project team is relatively tight on its own funds, it is even more impossible to entrust a sequencing company to do it.

But...... If we can get an order from the hospital, maybe the company will change from the current "serious shortage of operating rate" to "everyone is overworked to death". And for these "professional experimenters" who work for sequencing companies, that is called true happiness.

Without enough workload, the company can't even pay a stable salary. For a newly established sequencing company, it was a matter of life and death.

Hanging up the phone, Sun Lien heard a crisp voice of a little girl in the corridor outside the office.

"Little rabbit, open the door......"