No. 2333, Jiangzhou Science and Technology Park, Institute of Cryptococcal Disease.

It has been more than half a year since its establishment last year.

But so far, nothing has been found.

The reason has also been said before, it is too difficult to clone cells because the amount of antigenic B cells in serum is too low.

After isolation of B cells, the sensitivity and specificity requirements for their sequence amplification and determination are also very high.

Even if the sequence is amplified and the antibody is expressed, the "neutralizing effect" of the antibody needs to be further identified.

In short, it is extremely difficult to replicate antigenic B cells.

Of course, all of the above is for the average person.

Not including Zhou Wen.

He has now upgraded to LV4 in several subjects, and he can almost start trying this task.

This mission brought Zhou Wen not only experience, but also some other additional things, such as influence in the academic world, which other tasks could not bring him.

On the afternoon of May 5, Zhou Wen officially started cell cloning research.

The first step is to isolate B cells.

This is difficult for others, but for Zhou Wen, who has a true eye, it is a piece of cake.

First, his eyes can directly see the antigenic B cells in the serum;

Secondly, the upgrade of biological immunology to LV4 brought him not only a simple amount of knowledge, but also a deeper understanding of antigenic B cells, and he integrated a set of unique isolation methods.

These techniques are independent of existing biological knowledge.

A week later, Zhou Wen successfully isolated cryptococcosis antigenic B cells.

Li Feng and Fan Bo, who have always had a lot of complaints about Zhou Wen in their hearts, almost didn't pop out their shocked eyes.

The other 13 doctoral researchers in the laboratory were also shocked.

Everyone's admiration for Zhou Wen in their hearts is like a surging river, endless, and like the flooding of the Yellow River, it is out of control.

But no one cheered!

This is just the first step in the long march.

Now there's the main play, "Sequence Amplification"!

This is also difficult for the average person, because antigenic B cells are so scarce, and the failure rate is as high as 99% when the sequence is amplified!

And if you fail, you probably won't have a chance again.

But this is not too much of a problem for Zhou Wen.

Don't forget, what did Zhou Wen start with, DNA extraction and amplification!

In the world of DNA extraction and PCR, he thinks he is the second, and no one dares to claim to be the first.

Of course, Zhou Wen didn't dare to take it too lightly, so he still cheered up and went all out.

Because there are so few specimens.

If it fails, the isolated B cells will be invalidated, and it will take a week to re-isolate, and it may not be a one-time success!

The DNA amplification procedure is much the same as that of a mouse, except that he has redesigned the primers himself.

A number of simulation experiments were also conducted.

Six days later, Zhou Wen successfully expanded a sufficient number of antigenic B cells, and after product analysis, it was determined that the expansion was successful!

There is no shortage of research samples.

The whole human laboratory is boiling!

Everyone looked at Zhou Wen as if they were gods.

If the one-time isolation of antigenic B cells still belongs to the category of humans, then the one-time expansion of antigenic B cells without any experience and no chance of failure and restart is completely non-human technology.

At this moment, Zhou Wen has become their spiritual belief!

Even if he now says that he is an alien, they are convinced!

This is followed by antibody expression assays.

Antibody expression is a multi-step process that begins with transcription from DNA to mRNA and reaches a high-tide when mRNA is translated into protein......

The 30,000-word introduction is omitted here.

Not surprisingly, the sensitivity of antibody expression is naturally a one-time pass.

Li Feng and a group of doctors were not surprised by this, and even took it for granted.

The next step is the most important and final step of the antibody "neutralization" test.

Because the "antibody" itself only has the function of recognizing the target antigen, and does not have the effect of killing or rejecting the target antigen.

The "neutralization effect" is that the antibody binds to the antigen to produce a precipitate or cell group, and then is engulfed by phagocytic cells, and then the antigen is killed!

If the neutralization effect test is successful, it means that the vaccine for cryptococcosis has been successfully manufactured.

......

Sangya Hospital, Office of the Director of the Department of Infectious Diseases.

Cheng Yanqing was writing the experiment report when the phone on the table rang.

She picked it up and looked at it, it was Pan Renfeng, a member of the group in the virus exchange home and director of the infection department of Harmony Hospital.

"What's the matter, Director Pan?"

Zhou Wen said that the antigenic B cells they expanded have been tested for antibody expression and will be tested for neutralization effect soon. ”

Cheng Yan paused for a moment, and then said in shock: "What did you say?"

"I said that Zhou Wen and they have isolated and expanded antigenic B cells with cryptococcosis, and the next step is to test the neutralization effect. ”

"Impossible!" Cheng Yanqing said categorically, "I called the institute at the beginning of the month, and basic research is still being done there, which is impossible." ”

Pan Renfeng said: "This is not what I said, this is what Zhou Wen said himself, you go to the group and take a look." ”

When Cheng Yanqing heard this, he immediately opened the WeChat group, and he really saw Zhou Wen's speech.

It was the old house of the Xia family who asked Zhou Wen what he was doing recently, and Zhou Wen casually replied that he was cloning antigenic B cells for cryptococcosis.

Then the old house of the Xia family asked him how his research was?

Zhou Wen said that the neutralization effect test will start immediately.

Zhou Wen's words blew up all the big guys in the group who dived and peeped at the screen.

All were incredulous.

But after Zhou Wen posted a few test data sheets, the group suddenly exploded.

Cheng Yanqing clicked on the test data and looked at it, shocked beyond measure!

She wondered if it was April Fool's Day.

How could such a thing exist?

She called Li Feng at the beginning of next month, and he sighed and said that there was no progress, and his words revealed his complaint to Zhou Wen.

But now......

Cheng Yanqing immediately called Zhou Wen, who was on the phone.

Cheng Yanqing didn't hang up the phone, but kept waiting.

After about a few tens of seconds, the phone was connected.

"Zhou Wen, what's going on?"

"That...... I can't explain it clearly for a while, so let's do it, Director Cheng, if you have time, come to Jiangzhou tomorrow and have a face-to-face interview. ”

Cheng Yanqing had too many things to ask in his heart, but at this time, he didn't know where to start at all, so he had to hang up the phone for the time being.

How could Yanqing wait until tomorrow to go if he didn't?

didn't say a word, and immediately set off for Jiangzhou.

On the way, I called Li Feng, the person in charge of the laboratory, again and again.

Finally, it was finally opened.

Under Li Feng's narration, Cheng Yanqing's jaw almost didn't drop, and he couldn't come back to his senses for a long time.

"Doodoo ————"

Finally, Cheng Yanqing was awakened by the sound of the horn behind him, and only then did he realize that he had stopped at the intersection of the traffic light.

Cheng Yanqing took a deep breath after starting and asked, "Why didn't you tell me about such a big thing?"

Li Feng said: "Because Zhou said, the process is not important, the important thing is the result, if the neutralization effect test is not passed, all the sweat and effort before are meaningless, so there is no need to let everyone know." ”

Cheng Yanqing: "......"

Is this human speech?

The successful isolation of antigenic B cells is a remarkable feat, and it also has a very important reference role for the isolation of antigenic B cells from other rare diseases!

In her opinion, this technology alone is enough to be worth three Lancets!

How can it be said that it is meaningless?

As for the subsequent sequence amplification, it is also very important.

"This little guy is so unbelievable!"

Cheng Yanqing said a word, and couldn't help laughing.

......

Sunday, May 26, 2019.

At half past nine in the morning, the Jiangzhou Institute of Cryptococcosis conducted an open test on the neutralization effect of antigenic B cells in cryptococcosis.

Some good friends and leaders who are familiar with Zhou Wen visited the site.

Like Pan Renfeng, Cheng Yanqing, Gu Yunwen, Mu Yuanqing, Lin Huaidong, Zhang Wen, as well as several leaders of Jiangzhou Medical College and respected experts and professors, etc.

The results were not surprising.

Under the attention of everyone, the group of cells produced by the combination of cryptococcosis antibodies and cryptococcosis antigens was swallowed up by phagocytes little by little!

"Bang Bang Bang-"

In the observation room outside the laboratory, there was warm applause!

"Congratulations Director Cheng, you guys are really amazing. ”

"This is a great invention! Director Cheng, you guys are so good!"

“......”

Facing the praise of a group of old friends, Cheng Yanqing said with a smile: "Okay, okay, you don't have to tout here! I am also a leader, and all the research work is done by Zhou Wen, and it has nothing to do with me." ”

The experts on the scene knew what was going on, so everyone was smiling.

It doesn't matter if friends joke with each other, but if you really tear your face and steal other people's academic achievements, they are not so cheeky!

And this one can't be snatched away at all.

There are several core technical processes in it, and when the experimental restoration can't be done, the shame will be thrown to grandma's house.

The experts were embarrassed, but one of the leaders of Jiangzhou Medical College laughed and said, "Director Cheng is too modest!"

Xiao Zhou's ability is undeniable, but we can't ignore the role of leadership, without the leader's instructions and proper scheduling, they young people can only spin around like headless flies, right?"

Cheng Yanqing was really embarrassed when he saw this leader's serious face, and said secretly in his heart: "I'm not modest, please brag and don't bring me!"

Seeing the expression on Cheng Yanqing's face, Pan Renfeng, Lin Huaidong, Zhang Wen and others couldn't help laughing.

And the pot-bellied leader of Jiangzhou Medical College was inexplicable in his heart, and secretly said: "Is my words funny?"

But he didn't dare to question it, let alone put on a face.

Pan Renfeng, Gu Yunwen, Lin Huaidong, Zhang Wen and others, which one is not a bigwig in the domestic medical system?

That's the one who can talk directly to the head above.

With his level, it's not enough to see at all......