As a provincial key laboratory, the laboratories here are as well-equipped as the laboratories where Lu Cheng had been before.

Of course, the completeness here only involves in vivo experiments, that is, cell experiments.

Animal experiments require particularly strict stratigraphic flow space, which also requires special construction of special laboratories.

Lu Cheng heard before that all the treasures raised in the animal laboratory were treasures, and if he could raise forty or fifty mice, it would definitely be at the level of a big man, and the annual cost of hundreds of mice would not be affordable by the salary of ordinary doctors.

The laboratory is divided into the following places.

The cryopreservation room, where cells and tissue specimens are preserved, is one of the most important places in the laboratory.

The cell culture room has an operating table, a centrifuge, a 37° incubator, and a liquid nitrogen tank on the outer layer of the laboratory for cryopreservation.

PCR, flow cytometry, and more.

Basically, all the operations involved in cell experiments can be done in this laboratory.

Lin You is an old acquaintance in the laboratory, and when someone saw Lin You, they greeted him by the way: "Brother You, are you here to do the experiment again?"

"Well, wake up some cells, and bring the students by the way. ”

"Brother Ming, this is Lu Cheng, if there is anything he doesn't understand in the future, you can teach him more. Lin You took the initiative to introduce Wang Liming.

Wang Liming is a doctor of dermatology, but Lin You is also doing experiments in the laboratory, and of course he knows what level of articles Lin You has posted before.

And Lin You had never been a student before.

Wang Liming immediately replied: "Brother You, you have just been hospitalized, and you have a big disciple? ”

"Okay. In the future, I will definitely learn from each other with Lu Cheng's junior brother. Wang Liming was not particularly arrogant in front of Lu Cheng.

Lu Cheng also hurriedly greeted and said, "Brother Ming, will I trouble you in the future?" When you are doing experiments, if it is convenient to call me, I can fight and take a look." ”

Wang Liming is to give Lin You face, of course Lu Cheng will not climb up along the pole and put his position as a novice correct.

Wang Liming already had a lot of boss's projects to do, and after hearing Lu Cheng's words, he looked at Lin You and joked: "Brother Da You, I really have something to do for him." You won't blame me for calling your students in private, will you?"

"I just came, and I have to polish it well. Hard work Brother Ming. Lin You originally just asked.

I didn't expect Wang Liming to really have an operation for Lu Cheng to do, so it is the best, it can further consolidate Lu Cheng's experimental foundation, if he follows Wang Liming to run a set of experimental procedures, he will be able to do experiments by himself in the future, and he will be able to do it easily.

Wang Liming is a doctor, he is the director, and the free time of the two, who has more and who has less, can be imagined with his ass.

The doctor was originally immersed in the laboratory.

"Oh, that's what I said, let's have dinner together next time. Today we will first go to the cell room to have a look. Lin You admitted his feelings and offered to invite him to dinner.

Wang Liming has something to do for Lu Cheng, and he happens to bring students to him, and Lu Cheng happens to learn things, things that benefit from three benefits.

After Lin You finished talking to Wang Liming, he took Lu Cheng to his cabinet.

In the cabinet, there are all kinds of instruments and reagents he purchased, which are all for his exclusive use, and others generally do not borrow them. But if he wants to borrow someone else's, ordinary people will not refuse.

As the presiding officer, this privilege is still there.

Lin You generally pointed to the cabinet and introduced to Lu Cheng: "These two layers are all the belongings of the master here. ”

"How's it going, did you see what you need to use to raise cells yesterday?"

At this point, Lin You did not deliberately teach Lu Cheng.

Being in the lab does not mean that you have done experiments, after all, cell lines are very expensive, and most people are reluctant to give others the opportunity to do it.

In case of pollution, thousands of oceans.

Even if it is a culture medium, a small tube is dozens of oceans, and it is very expensive.

Lu Cheng didn't deliberately show off, just swept it into the cabinet, and took the initiative to put a 5ml culture flask and a 10ml centrifuge tube. Pipettes of various sizes were removed.

Immediately afterwards, Lu Cheng rummaged through it again and found the tip of the pipette.

Holding it all in his hands, he thought about it again: "Master, if we just awaken cells today, these should be enough. ”

Lin You was slightly surprised, but Lu Cheng had entered the laboratory, so it was reasonable to know this.

However, Lin You took the initiative to put down the 5ml culture flask in Lin You's hand, and then took out a 15ml culture flask.

And put the pipette tip back down, and take out the electronic pipette and electronic pipette tube.

And with a smile on his lips, he said, "I haven't used this thing before, have I?"

Seeing Lin You take out the electronic pipette and pipette, the corners of Lu Cheng's mouth immediately twitched, and he said with a slight wooden flute: "Master, this guy's pipes are one or two pieces by piece?"

"What's the matter? Think your master doesn't have any money?"

"I'm telling you, that's what we're going to do, it's the most standard, it can reduce the number of fluid transfers and maintain sterility to the greatest extent. ”

"Come with me and take you to get the culture fluid, pancreatic enzymes, and PBS. ”

"Do you know what PBS is?" Lin You gave the things to Lu Cheng and asked as he walked.

"Phosphate-buffered saline solution, when we wake up the cells, there is cryopreservation, and after awakening, the cryopreservation solution will damage the cells, so we can rinse and centrifuge with PBS and culture medium. Cryopreservation liquid washing, that would be too luxurious. ”

"Well, sometimes you can also wash it with PBS when you change the fluid. Lu Cheng entered the laboratory and replied without hesitation.

"Yes, it seems that I really worked hard. ”

"Let's go sterilize the equipment, and then warm up both the culture and PBS. Lin You then took Lu Cheng directly to the cell room.

The second platform inside was made by Lin You in advance, and it happened to be this time period, so someone reserved it for him, otherwise, it would definitely not be so coincidental.

After entering, put all the instruments in and must be disinfected with ultraviolet light for half an hour.

After this, both of them had to wait for half an hour, so Lin You really asked for real academic knowledge.

"Have you ever known about bone tumors before?"

"Well, after I told you about our future research direction?" Lin You said in a slightly lower voice, and then added: "It's okay if you don't know, no one leads the way, this road is difficult." ”

How could Lu Cheng not understand?

It can be said that the biggest purpose of his coming to study medicine is for this thing, and understanding is of course the foundation.

Lu Cheng nodded, knowing is knowing, anyway, he didn't need to have any reservations in front of Lin You.

He will not rob Lin You of anything, and any of his merits are Lin You's wealth.

"Understood. ”

"Our current research direction is energy metabolism in osteosarcoma. ”

"Specifically, it is the LTH enzyme, the last step of glucose metabolism, and the associated genes. ”

"The current mainstream research knows. ”

"Tumor cells, especially osteosarcoma cells, are mainly metabolized by anaerobic metabolism, so sugar metabolism does not switch to aerobic metabolism. ”

"This is the main way tumor cells use energy. ”

"LTH enzyme is a key enzyme in the shift of sugar metabolism to lactate metabolism. If this enzyme and related genes can be clearly understood, the energy supply of tumor metabolism can be cut off to a large extent. ”

"Moreover, our normal human body, as long as there is no strenuous exercise, the activity of LTH enzymes is very small, and there are no side effects and reactions to the energy metabolism of our normal cells. ”

"On this basis, we can further search for the main mRNA that promotes the synthesis of LTH enzymes, and more deeply, we can start from Linc RNA and other aspects......"

Lu Cheng knew that this must be the place where his dream set sail, and at this time, it was also his first step into that field.

Lin You is a prophet and a guide for him, and he must unreservedly bring out his knowledge reserves at once to make enough contributions to the team in order to be able to stand firm in the team......

(Attention to the party, the various pathways and corresponding genes in this book have nothing to do with reality.) The main reason is that I am afraid that I will leak some things of the team if I write high, which is the only principle. Therefore, any related genes, RNA names, and so on in this book are fictional and have nothing to do with reality.

Again. Don't be so meticulous. Thank you. But there must be logic in the research methods and ideas inside. If there is a particularly rigorous SCI special study party, please take a detour, thank you. However, you can communicate and discuss with each other in the group, which is welcome. ）

(Set the bowl, pitifully ask for a collection and recommendation, reward.) ）