Even if you make a mistake, no judge will be able to see it.

Lu Cheng's words are a bit exaggerated, not quite in line with Lin You's scientific research concept, in his opinion, the data in the article is, of course, true and effective, rather than relying on data processing capabilities to hide from the world.

But in the same way, Lin You, who has seen Lu Cheng's ability to use and control terrifying data, is also powerless to complain, he is also reviewing for the magazine, and it is difficult to find any data problems, and he can only find problems in the structure and logic of the article.

This aspect is the biggest problem of an article.

Lin You hurriedly waved his hand and said: "Just say this in front of me, don't say it, otherwise if the media knows about it, you will probably be half ruined." ”

"Well, according to what you say, you've raised cells before?"

"I've raised. Moreover, it is mesenchymal stem cells, which should be more valuable than tumor cells. Lu Cheng said honestly.

When he was at Zhonghan University, he couldn't get into the laboratory of bone tumors, and there was no way to do it.

When Lin You heard the words mesenchymal stem cells, he immediately moved his buttocks and said: "After that, I will rest, today's recovery and passaging, you can do it." ”

Nourishing cells are also divided into levels and types.

The best cells with the strongest vitality belong to tumor cells, and osteosarcoma cells are known for their strong vitality.

Secondly, there are some ordinary cells, whose multi-growth ability and viability are far less than those of tumor cells, and belong to the regular type.

Some special cells, such as mesenchymal stem cells, require a special environment, unlike tumor cells, which grow when you eat them.

Lu Cheng can get that thing, and it is naturally not difficult to raise tumor cells.

"Dididi!"

As he spoke, the indicator light on the cell console went off.

This is the operating table is disinfected.

"Follow me to identify where our cells are in liquid nitrogen. ”

"Today we are going to resuscitate a vial of the HOS and SaoS2 cell lines. These two cell lines are raised for you, raised for a period of time, and then a seed is saved, and after the seed is saved, a part of it will be used for animal experiments. ”

"No problem, right?" Lin You said to Lu Cheng.

Lu Cheng has never done animal experiments, so he doesn't know whether Lin You is asking is okay with cell experiments or animal experiments.

"I try to take care of them as much as I can. ”

"No. 2 liquid nitrogen tank, No. 4, the penultimate small box, is our cell line, this is your master's treasure for many years. Don't lose it for me. Lin You cautiously explained.

Cell lines that can be used for experiments are not something that can be obtained from a patient casually.

These cell lines, when they are introduced, require thousands of oceans, and they need to be a little less, which is why they must be stored after every experiment.

Moreover, tumor cells are not infinite and can be passaged, generally in the current mainstream, tumor cells can only be passaged ten times at most, and can no longer be used in experiments, because the variability after ten times of multiplication is too large.

Therefore, the more primary cell lines are, the more attention is paid to them.

"Alright, Master. Let me do it, this liquid nitrogen, very cold. Lu Cheng took the initiative to open the jar of the liquid nitrogen tank, then took out the cryopreservation box inside, counted to the penultimate layer, endured the heat of the negative temperature of liquid nitrogen, and finally got out the two cell lines of HOS and SaoS2.

Then, Lu Cheng hurriedly put them back again, and said to Lin You: "Master, I'll put this box in again, you can take it and revive it first, right?"

Lin You immediately took it and asked, "What is the best time for recovery?"

This is another exam.

Lin You's habits.

Lu Cheng put the box and said, "The sooner the better." ”

Lin You entered the door and was relieved. If Lu Cheng casually said a time, even if it was within ten seconds, it would be nonsense.

When Lu Cheng came to the cell console again, Lin You had already recovered the cells, and Lu Cheng hurriedly took out the heated culture liquid and PBS liquid from the constant temperature water tank, and then disinfected them with alcohol and put them into the console one by one.

Resuscitation first.

Use a suction tube to transfer the cells in the cryovial tube to a 10ml centrifuge tube, and then add about 5ml of culture medium to the centrifuge tube at the same time.

Two tubes, almost without trimming, can be centrifuged directly.

Remove the supernatant and re-pipette with the culture medium.

Add to the flask with 5 ml of culture medium......

Pour out the old culture medium, PBS rinse, trypsin, culture medium......

All of Lu Cheng's operations were done in one go.

In about twenty minutes, a total of six cell culture flasks were filled with resuscitated and passaged cells.

"It's okay, if I see something unclean about you halfway, I'm going to hit your hand, but it's good that you didn't give me this chance. Lin You said with great satisfaction.

"It's okay, Master. When we do experiments, don't we generally need at least three cell lines?" Lu Cheng asked with a little confusion.

"Don't worry, another MG63, others help raise it, your master's time before is really not enough, and when you have time in the future, you can go and get the bottom seed back." ”

Lin You bent down slightly, looked at the time, and it was close to seven o'clock.

"Let's go, go back. Come home with me for dinner?" Lin You and Lu Cheng asked as they went to put on their white coats.

Lu Cheng smiled and shook his head: "Thank you, Master, it won't be troublesome." I'll go home and read a book for a while. ”

Going to Master's house for dinner is of course a good thing, but this is also too fast, Lu Cheng can't be so direct.

Anyway, it's going to be Teacher's Day, and after meeting with Master and Master, I will think about similar things.

Otherwise, Lu Cheng himself felt that he was reckless.

Of course Lin You will not force it, Lu Cheng goes, he will not have any ideas, if he does not go, naturally there are Lu Cheng's own considerations, and even Lin You feels that he is too concerned about Lu Cheng.

Of course, to be attentive to a person, first of all, this person must make you more worry-free, so that you have time to pay attention.

Otherwise?

Lu Cheng had already left Lin You's line of sight.

The phone rang again.

When I opened it, it was still sent by Lin You: "Xiaocheng, I can't be in a hurry to write an article, but I can't delay it." ”

"You should make a rough plan for the near future, and it is a good idea to list the weekly and monthly plans. ”

"I also need to make time for the rest of my time. ”

Lu Cheng belonged to the first time writing the article, and Lin You was still a little uneasy, so he wanted to help check it when the time came, so that he could at least take a little less detours.

"Yes, Master. ”

Lu Cheng replied.

Then he sent a relatively full schedule to Lin You.

Lin You has always had the habit of replying to messages in seconds, and immediately replied: "Xiaocheng, isn't this time too tight?"

"A week, make an article and submit it??"

"It's not as easy as you think. ”

"It's okay, Master, let's take our time, don't rush. ”

"Gotcha. That Xiaocheng, I'm going to do animal experiments this month, do you have a pass?"

"Master, I have registered in the animal laboratory, and I will be able to go there after the training every Friday morning, and I am going to take a leave of absence this Friday, and then transfer the patient to my distant brother. ”

"Okay. At home, Lin You suddenly covered his forehead.

What kind of freak did he take?

People's masters, are urging apprentices to do that every day, their own demons, they are urging themselves to do a meta a week, and then before the end of the month, they have to do animal experiments, he really doesn't know how thick the sky is!

But it's okay to let him eat and eat, so as not to be high and low in the future.

No, it's too arrogant.