After Jiao Zhengde learned that Bai Ye wanted a high blood pressure model, he called a man, and ten minutes later, a man with glasses about thirty years old walked in.

"Teacher, what did you call me for?" the man asked.

Jiao Zhengde introduced: "This is Bai Ye, you have to learn modeling here during this time, you take him to study hard." ”

"This is Dong Cunguang, my student, who has made some achievements in the field of mouse modeling research, if you have any questions, just ask. ”

Bai Ye's laboratory was arranged next to Dong Cunguang, so it was convenient to take care of something.

Dong Cunguang is a relatively orthodox person, the glasses he wears are all upright, although he does not smile at Bai Ye, he responds to requests.

In the following days, Bai Ye did not make a model by himself, but studied with Dong Cunguang, and then read books in private.

Zhang Hanlin needs two kinds of mice, one is essential hypertension and the other is renal hypertension, and his main research is the clinical research efficacy of essential hypertension and secondary renal hypertension.

Dong Cunguang is cold and hot-hearted, maybe not cold, but not sociable, Bai Ye only realized his extraordinariness during the time he was in contact with him.

At the age of 31 this year, he has published 8 SCI papers, and his topic: improved rat renal hypertension modeling won the second prize of Kyoto Youth Scientific Research Progress Award.

However, Dong Cunguang's family has four children, he is the eldest, and he can't afford to study.

And it happened that Tang Ya offered him an annual salary of one million at that time, and Dong Cunguang entered the company directly without the slightest hesitation, but this money was not spent in vain, and he successively improved several modeling methods, which brought a lot of benefits to the company.

In the past few days, Dong Cunguang is still modeling rats and observing changes in blood pressure, which is already his daily life, and the two have gradually become familiar with each other.

Bai Ye would go to the laboratory early every day to prepare it, and even take the initiative to do what he could.

Dong Cunguang also gradually began to communicate more: "Why don't you try it yourself?"

Bai Ye was stunned for a moment, and hurriedly nodded happily, after a few days of observation, he was already a little impatient to start.

Dong Cunguang stared at Bai Ye and said, "I have been working on a model of renal hypertension for the past few days, and you probably have an impression, you can try it today, and I will point out to you if there is any problem." ”

Bai Ye found a relatively large male SD rat, weighing about 180g, and first used scissors to remove the hair, and then disinfected it with iodine.

The gold-blatt hypertension model has a double kidney and a single kidney model, the double kidney is to retain both kidneys, but to give one or two renal artery stenosis, also known as double kidney and one clip or double kidney double clip, the single kidney model is to remove one side of the kidney, stenosis to retain the renal artery, that is, one kidney and one clip.

Double kidney and one clip is the most widely used, the most classic, and the most recognized hypertension model manufacturing method in the world, and the model is simple, the success rate is high, and the mortality rate is low.

Bai Ye made a longitudinal incision of 1cm to the left of the abdomen of the rat, 3-4cm long, and the anesthesia was 10% chloral hydrate, and 1ml of 180g body weight was administered intraperitoneally......

However, to Bai Ye's embarrassment, the mouse died......

Dong Cunguang looked at the embarrassed Bai Ye and said quietly: "He's dead, there's no need to cut it anymore." ”

Bai Ye didn't think anything about it, but after listening to Dong Cunguang's words, he actually felt a sense of guilt for whipping the corpse......

Dong Cunguang said lightly: "The success rate of this kind of modeling is very high, the mortality rate is very low, less than ten percent, and the most likely problem is anesthesia, when you are anesthetized, if I don't guess wrong, the place where you inject may be the heart." ”

Bai Ye: "......"

………………

However, everything is difficult at the beginning, after a few days of study, Bai Ye has gradually been able to complete the mouse modeling by himself, and the success rate is very high.

In the past ten days, Bai Ye made nearly 100 of them, and Dong Cunguang made 500 of them, and this was just what he did casually......

These days, Bai Ye and Dong Cunguang have gradually become familiar.

Bai Ye thought of something, so he asked, "Do you have any thoughts on essential hypertension modeling?"

Dong Cunguang pondered for a moment and stopped what he was doing: "It's not easy to do, the main reason is that the results are not uniform, the individual differences are relatively large, and the cause of essential hypertension is unknown, so there are many methods in the process of modeling, such as nervous, spontaneous hypertension and so on." ”

When Bai Ye heard this, he suddenly thought that the new type of modeling in the rat raising experience requires the gene of high-pressure mice, so he asked: "Is there a mouse with a high-blood pressure gene?"

Dr. okamato mated a male istar mouse with a blood pressure of 19.3~23.3kpa and a female mouse of the same species with a blood pressure of 17.3~18.6kpa, and its offspring selected a person with high blood pressure of more than 24KPa, they called it spontaneous hypertensive mouse (SHR), through continuous selection, in 1969, the inbred line SHR was obtained, and by 1986 the line had been passed down to 80 generations. And the technology is very mature. ”

Bai Ye's eyes suddenly lit up, because in the experience of raising rats, the modeling of essential hypertension mice requires mice with high blood pressure genes for gene replacement, so after knowing this kind of mouse, Bai Ye was also happy: "Then how high is the proportion of hypertension among them?"

According to statistics, in the 30th ~ 32nd generation, the average blood pressure of male mice in the tenth week is 24.5±2.3kpa, and the average blood pressure of female mice is about 23.7±1.9kpa, and it will continue to rise thereafter. ”

Speaking of this, Dong Cunguang shook his head: "The price of an SHR is about 500 in China, but the effect of SHR made in China is very poor, and the prevalence is extremely high. Our company also did training at the beginning, but unfortunately the core technology of SHR cultivation can not be transmitted, and there is a big gap between the SHR we cultivated and the SHR of Longka, how to say it, if we use the BPFH score that has prevailed recently, the best score we have cultivated is also at the second level, and the worst of Longka is also in the second class, which is generally the first class. ”

When Bai Ye heard this, it was no wonder that Zhang Hanlin had been buying foreign rats, the original reason was here, thinking of this, he couldn't help but ask: "Then there are no other rats?" ”

Dong Cunguang suddenly smiled, very rare: "Yes! but...... Immature and still in research. ”

Bai Ye suddenly found that this was the first time he had laughed since he met Dong Cunguang, right?

"What's the solution?"

Dong Cunguang's eyes were full of light: "Genetic engineering, I have been studying the genetic sequence of SHR recently, if I can find the difference between him and other mice, maybe I can learn from it, so that at the embryo level, I can use the shotgun method to change the genetic sequence of ordinary embryos, and maybe I can get something new." ”

After Dong Cunguang finished speaking, he was full of expectations: "One day, we will break this disguised technological monopoly and let the gringos buy our mice!"