Chapter 1436: Corring
Wang Liangcai sat on the bed, staring at the envelope he had retrieved from the finance office, unwilling to take his eyes off for a long time.
The envelope was thick, thick enough to make one doubt life.
JMC's paper prize will not be available until the paper is officially published, so Wang Liangcai is still a little disappointed.
However, the prize money of the achievement award is obviously not comparable to the prize money for publishing a paper.
It was only when he saw this envelope that Wang Liang realized that the laboratory attached great importance to the results.
After all, the completion of a single topic often represents multiple heavyweight papers. And papers, if you want to have more, just brush more, they are all dependent on the results.
"One is Langyuan Immortal, the other is beautiful and flawless, if you say that there is no strange fate, I will meet him again in this life......"
The opening song of "Dream of Red Mansions" came from next door, and Wang Liangcai woke up violently.
The newly released "Dream of Red Mansions" can be called the most attractive TV series of the year, and the so-called empty alleys of 10,000 people are not an exaggeration at all.
When the opening song of the TV series sounds, you can hear the sound of shouting home in the yard, adults and children who are crazy are easily called home, you can't go home if you don't go home, your friends who play with you are going home to watch "Dream of Red Mansions", who will play with you.
There is no TV in Wang Liangcai's small suite, this is the only regret, Wang Liangcai went to the cafeteria to watch the big TV two days ago, but today he was a little lazy to run.
He reached out and grabbed the envelope on the bed, poured out the brand-new bills inside, counted them again, then took out most of them, put them back in the envelope, and thought to himself: My wife has wanted to change the color TV for a long time, or else she would have exchanged this money this year.
For the rest, Wang Liangcai divided it into three parts, preparing to leave one for the family to improve the food, another to buy good clothes for his wife and children, and finally, to buy himself a few good books.
The distribution was completed, and looking at the four stacks of money in front of him, Wang Liangcai was still in disbelief.
He has never had a monthly income that can do four things.
Not to mention four, even a quarter, it's all remarkable.
For example, if you buy clothes, save some money every month, and then when you change the seasons, you can only buy clothes once, and you can't buy them for everyone in the family, you must be buying autumn clothes for your wife this year, and you won't have money to buy winter clothes.
It is even more difficult to change electrical appliances, saving money for a year or two to buy an electrical appliance is a common thing, during this period, there can be no big projects that spend money.
Therefore, when you go into ordinary people's homes, you can count the electrical appliances on one hand.
Wang Liangcai has no foundation at home, he has worked for many years, and he has become a professor, so he has bought a black-and-white TV, as well as an electric fan, tape recorder and single-barrel washing machine, which can be regarded as catching up with the national average, as for equipment such as refrigerators and air conditioners, they have not yet entered the scope of discussion.
Wang Liangcai couldn't help but touch the money with his hand, as if he was afraid that it would run away.
Turning his head, Wang Liangcai couldn't help but smile bitterly, he called himself a literati, but he didn't expect that he loved money so much.
"A gentleman loves money and takes it in a good way. Wang Liangcai clenched his fists and made a secret decision, so he went into the quilt with a pile of money and slept in the dark.
Four o'clock in the morning.
Wang Liang, who had slept for 8 hours, got up from the bed, washed up, and went to the laboratory.
The dormitory was very close to the laboratory, and when he arrived at the door, Wang Liang gave a brief explanation, and the doorman put him in.
Wang Liangcai rubbed his hands as he walked, and when he arrived at the laboratory, he felt that his fingers were soft, and then picked up the furnace that was pressed yesterday, added coal, and then changed into a lab coat, opened the instrument cabinet, and turned on the micromanipulator, inverted phase contrast microscope, piezoelectric pulse meter, and stereo microscope......
Super ovulation and egg retrieval can only be regarded as the preparatory stage for cloning sheep. Next, the real challenge starts with "denuclearization".
Compared with the first two operations, the latter has a stronger sense of "scientific attributes".
At the very least, there are more instruments used.
Judging from the price of various types of equipment in 87 years, there are only a handful of research institutes in China that can undertake this project.
Wang Liangcai inspected the instrument and prepared the nucleated liquid.
There are two kinds of nucleation operation liquids: sucrose-free and sucrose-free, which can also be mixed with various strange hormones, and each time you stir something into it, you can write a small paper.
Even the change in concentration can be seen as a small issue.
But in Yang Rui's genetic engineering laboratory, such a project is naturally unsightly.
Wang Liangcai only followed the traditional model and made a sucrose-free version and a 3% sucrose version. Immediately afterwards, he took out the Petri dish, dropped the nucleation manipulation, added a cell relaxant, then covered with paraffin oil, and finally, placed the oocytes of the sheep in the droplets.
It was just a small step in the beginning stage, which made Wang Liangcai sweat all over, and the cold in the laboratory was no longer felt.
This is also the academician-level Wang Liangcai, if it is replaced by other players, let alone a little newcomer, an elderly professor who is known for his operation, or a fledgling new professor, he may not be able to play these steps.
At the very least, you have to fail for a week or two to complete 100% of the set moves.
Considering that every failure in this involves wasting one or more oocytes, one can imagine the importance of experienced players.
Not to mention, the next steps are just more complex continuous movements, more testing techniques.
Tong Dizhou was able to get up from the Belgian cow back then, because his skills were good.
As for why his methods are good? There is a story dedicated to it: At that time, the Belgian people had already begun to commute to work on time, and the scientific researchers strictly enforced their work requirements, and after dinner, they went home to play, and Tong Dizhou soaked in the laboratory alone until two or three o'clock in the evening, until the security guards drove him away.
This training technique was also passed down with Tong Dizhou's return to China.
"Have you ever seen the capital at four o'clock in the morning?" is really not a problem for genetics scholars.
Otherwise, how to make a reputation for bitterness on the biological path of bitterness.
Wang Liangcai doesn't feel that doing experiments is hard, compared to metaphysical physics, suicidal chemistry, biology is finally normal, at least, he can improve his value through training.
The egg cells of sheep are too expensive, so they can be made into the egg cells of amphibian frogs as Comrade Zhou did.
However, a long period of training and concentration are certainly inevitable.
Not only that, it is difficult for any experimenter to remember every experimental step perfectly, let alone keep abreast of the latest research trends in the scientific community.
In this case, it is necessary to read a lot of literature constantly.
Even with the help of a data group, the time savings are still limited.
Wang Liangcai also read the operation guide first, and then adjusted the lens of the inverted phase contrast microscope, began to align the oocytes, and used the enucleation needle to gently wave to find the position.
After rubbing his hands to soreness, Wang Liangcai finally used the enucleated needle to stick to the cells with the help of the fixed needle, sucked the zona pellucida to keep the nucleated needle under negative pressure, and then turned on the piezoelectric pulse meter and tried to penetrate the zona pellucida with a strong pulse impact.
The nucleus is very small.
Cells are small enough, but the nucleus is much smaller than the cell.
Removing the nucleus from the cell is obviously not an easy task.
The success rate of the blind aspiration method currently used by Wang Liangcai is not low, but it is easy to suck up too much of the material in the egg.
The latter is an important nutrient for the development of the egg cell into the embryo, and if it is sucked away, the embryo will not develop completely, and it will still fail.
Wang Liangcai also knows such drawbacks, so he thinks while doing experiments.
Experiments are like this, there is no such thing as a perfect experimental method, and it is impossible not to continue because the method is not perfect.
He thinks of new methods, but if there is no flash of inspiration, it is not unacceptable to use stupid methods to make results.
Unless stupid methods consume too many resources, most of the experiments are done by countless stupid methods, connected by a few spurs of thought.
As the sky grew brighter and more people came to the laboratory, Wang Liangcai still forgot himself and continued to work.
From four o'clock in the morning to eight o'clock in the morning, although he worked for four hours, as far as the experiment was concerned, he only carried out four times.
And four times it failed.
For an experimental biologist accustomed to masochism a thousand times, four times is just an appetizer.
At nine o'clock in the morning, Gu Qiang Shi Ran came to the laboratory.
He didn't arrive late, but he chose to go to the cafeteria for breakfast first and come back after he had a full stomach, but it was the first time he saw Wang Liangcai who was working hard.
"It's too early. Gu Qiang couldn't help pouting, but the steps under his feet were three points faster.
……
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