It is difficult to enucle a cell.

It was difficult in the 50s, it was difficult in the 80s, and it's still hard in the 21st century.

It can be said that anyone, whether it is a bachelor's degree, a master's degree or a doctorate degree, as long as he can master the method of cell enucleation, he can easily find a job with a monthly salary of 3,000 yuan in any full-time university!

For example, Comrade Wang Liangcai, if he goes abroad now, he can easily find a teaching position with an annual salary of 40,000 yuan.

If you make an analogy, the work of enucleating and re-injecting cells is like doing heart surgery in a hospital, is there a more difficult operation than heart surgery? Of course there is.

However, when it comes to universality and popularity, it is heart surgery that is more eye-catching.

Wang Liangcai is the chief surgeon of cardiology in experimental biology.

His success rate is not high, but there are not many people in the country who can fiddle with the position of the cells under the microscope little by little, and then carefully suck out the nucleus.

Wang Liangcai worked in the laboratory for a few days, and everyone expressed their admiration.

The laboratory, like the factory, is the place where competence is best reflected.

Because everyone is doing real things, and the workplace is in the same place, whether the things made are good or not, you don't need to show off at all, you can see it with your eyes.

In fact, in order to show their eyesight, those who can see have to help with publicity.

Therefore, a strong man who does well in the laboratory, just like a good eighth-level worker or seventh-level worker in a factory, is easy to be discovered, and then it is easy to be touted.

In addition, Wang Liangcai is older and humble, and it didn't take long for even scientific research dogs to get used to asking him for advice.

"Professor Wang, can you help me take a look at this?"

"Professor Wang, there is always a white spot in the image I scanned electronically, can you help me take a look?"

"Professor Wang, I have a recent idea, this is how ...... come about"

"Professor Wang" was in his ears, but Gu Qiang was extremely uncomfortable listening to it.

Once upon a time, these questions were all consulted by other powerful people...... At least, part of the problem came to ask him for advice.

Although I thought it was very troublesome at the time, no matter how annoying it was, it was my own dog, so what's the matter with adding someone else's thigh now?

"Professor, I'd like to request a little more time. Gu Qiang couldn't hold back, bypassed the big work area, and asked Yang Rui for a policy.

Yang Rui looked down at the paper's expression forever, only raised his eyelids, and asked, "You don't have enough time?"

"Inverted phase contrast microscope, and in addition, the pulsometer, I also want a little more machine time. Gu Qiang hurriedly replied.

"Oh. How much. Yang Rui clicked the table with his hand and put down the paper.

It's normal to run out of time. It is impossible for any laboratory to supply an unlimited number of instruments, and even public laboratories rarely buy expensive instruments repeatedly.

In this case, sharing the machine is the best solution.

Of course, if Yang Rui was doing the project by himself, he would definitely use the opportunity to use it closely, but it would be impossible for Gu Qiang to have such good conditions for doing experiments.

He had to be like any other researcher, apply for the machine, and then conduct the experiment within the allotted time. If it cannot be completed by the expiration date, it cannot be occupied beyond the time limit, and it will have to be discussed with a later researcher.

Or, as it is now, apply for more hours.

Gu Qiang glanced at Wang Liangcai and said, "Four hours of microscope, two hours of pulse." ”

There was an inverted phase contrast microscope in the laboratory, and Yang Rui didn't need to look at it, so he said: "There are no four hours, and if you work during the day, you will be given two hours at most." ”

"That's not enough. Of course, Gu Qiang knew that there was no such a long time to get there. The inverted phase contrast microscope is currently a high-end product, and if you use it to brush papers, it is like playing with a SCI in half a month, although it is also a waste to choke.

However, in the genetic engineering laboratory, the use rate of inverted phase contrast microscopes is already high, and Wang Liangcai and others, who are actively working, want to spare 4 hours, which is basically unsolvable.

Yang Rui shrugged his shoulders and said, "You can follow Wang Liangcai's example, stay late in the evening, or come early." ”

No matter how expensive a microscope is, it can't be compared to a supercomputer or something like that, and since the genetic engineering lab hasn't swelled to the level of a department, there's no time to schedule in the early hours of the morning. Wang Liangcai rushed over early, which was equivalent to grabbing the extra opportunity.

Gu Qiang was intent on competing with Wang Liangcai, shook his head without hesitation, and said: "I want to do a set of coherent experiments, I want to connect the time of the day, if you give me a few hours, I will take a week." ”

"Have an idea for an experiment?" Yang Rui looked at Gu Qiang in surprise.

Gu Qiang said stubbornly: "Yes, it's almost verified." ”

"Is it enough to add 4 hours?" Yang Rui still has some trust in Gu Qiang's experimental ability.

Gu Qiang continued to say yes.

Yang Rui hesitated for a moment and said, "In this case, you will be tantamount to encroaching on other people's opportunities, you wait for me to ask." ”

Gu Qiang couldn't help but feel a little helpless. He also had to discuss with other people, and most importantly, with Wang Liangcai, such a result was not what he wanted.

Yang Rui did not call Wang Liangcai over to ask, but went directly to Wang Liangcai's test bench and watched his operation.

Wang Liangcai is doing the operation of the manifest nucleus.

This is the first step in the enucleation, and you have to make the nucleus of the cell visible before you can aspirate it.

If the nucleation experiment is compared to a happy amusement park, the nucleus is the step of buying a ticket and queuing, which is boring to death, just keep fiddling with the cells with the probe, so that they can be correctly fixed with the fixed needle, and then the fun part can be done.

Sadly, the nuclear experiment is not a happy playground, it is boring to death in itself, and how boring it is to imagine the nucleus.

However, Wang Liangcai is meticulous, and Yang Rui is also meticulous.

With him by his side, no one else came.

Two people, just like this, watched for nearly an hour.

Yang Rui finished reading the nucleus part from front to back, and couldn't help but feel a little emotional.

In another twenty or thirty years, there will be no essential change in the steps of denuclearization, and at that time, how many people can do it as well as Wang Liangcai?

The biology department of each school basically recruits one or two classes of genetics students every year, and after 30 years, can each school have at least one researcher who has done the experiment of cell enucleation?

That, of course, is impossible.

Just like most hospitals, there are no cardiologists who can do it.

They may have one or two good doctors who can do it, so that they can form one or two decent departments, just like most schools have one or two specialties that can be handled.

But that's it.

As for Wang Liangcai's operation, it is far more than he can handle.

Relying on the level of experimental biology, how powerful is the person who makes it to the academician selection? If you tie a white-faced sheep and tickle him, he can make the sheep laugh for the rest of his life.

"It's all standard operation. This is the reason why Yang Rui sighed.

It seems that everyone knows how to operate the standard operation, but when you really ask, there are a few people who can really make it all.

Just like an athlete on a basketball court, can a professional player make a standard operation?

If that's the case, are the varieties that shoot less than 50% from the field growing on trees?

Compared with players who rely on talent like Gu Qiang, practicing standard operations step by step not only requires more resources, but also means more effort.

Sometimes, if you can make good experimental results without standard methods, and you can change all your operating habits, you have to be ruthless to yourself.

Wang Liangcai didn't notice the appreciation attached to Yang Rui's semantics, and said with a smile: "That's how it is when we do more, we used to have nothing to do when we were in school, so we went to the laboratory to peel the eggs." The test bench is not enough, so I prepare for the experiment, and I am familiar with it when I come and go. ”

"I look at your experiment records, the success rate has reached 30%?" It's like there is a notebook next to the bedside, and there is also a notebook next to the test bench to record simple data.

Of course, those who can do this kind of detailed recording have to be high-end laboratories such as genetic engineering laboratories, ordinary laboratories, just like going to city and county hospitals, it would be good to have a dirty notebook to put out.

Wang Liangcai was neither proud nor discouraged by the 30% success rate, and said calmly: "Yes, I want to achieve a success rate of about 50%, and then continue to do the next step." ”

Manifestation, denuclearization and nuclear injection are said to be three major steps, and they must be completed in one go.

According to the experience of predecessors, these three steps can have a success rate of a few percent, which is considered good.

However, the success rate is calculated by multiplication, if the success rate of each step of Wang Liangcai is 30%, then after three steps, there will only be 2.7% left, which can reach the passing line.

Considering that the more difficulties there are in the future, it is necessary to strive for more if you can strive for a high success rate in the front.

Yang Rui nodded and said, "It's a good job." I've heard you've been coming to the lab a lot lately?"

"Yes......" Wang Liangcai replied not very firmly.

Coming early is not necessarily a good thing, the laboratory discipline is strange, and as a seconded officer, he is always a little uncertain.

Besides, if you go to the gymnasium at four o'clock in the morning to strike iron, will you be appreciated? What if it affects the lingering of coaches and managers?

"I want to adjust the machine, if you are willing to come to the laboratory earlier, how about I leave the morning time for you?" Yang Rui's answer was a little unexpected by Wang Liangcai.

"Can you give it to me?"

"Well, just press your previous time, or do you want more?"

"Can you ask for more?"

"I'll give you until one o'clock at noon, okay?"

"Yes, you can. Wang Liangcai was overjoyed.

The morning time is undoubtedly the best time to experiment. Not to mention, the state of all the instruments is also the best, because at the end of the day's experiment, all the instruments that can be reset to zero will be zeroed, and the next day there will be less time for adjustment, which is both time-saving and comfortable.

A few days ago, Wang Liangcai came to the laboratory in the early morning, and at eight or nine o'clock in the morning, he had to vacate the microscope that was assigned in organic time, such as an inverted phase contrast microscope.

Now that the time is connected, it's much more convenient for him.

Gu Qiang was completely helpless when he listened to it, he knew that Yang Rui readjusted the time to free up a whole piece of time for him, but ...... Don't give me time in the morning for Mao?

"It seems that the time has come to prove yourself with strength. Gu Qiang silently clenched his fists.

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