"PCR is worthy of being a Nobel Prize-winning technology, it's so easy to use. Pen ~ Fun ~ Pavilion www.biquge.info" Ouyang Shi stood in front of the PCR machine, just like a hungry person standing in front of the barbecue grill, his face was full of gentleness.

Yang Rui stood in front of him with a smile and watched Ouyang Shi operate.

He was injured when he played PCR in his last life, and he didn't do it if he could.

Gu Qiang didn't have Ouyang Shi's bones, he was across the experimental table, while operating the microscalpel, he laughed twice, and said: "You got this, it's not technically difficult at all, it's boring...... Xiao Liu, take it. ”

He divided another set of embryos and called his assistant to send them away.

Xiao Liu is also a researcher in the laboratory, but his level is not enough, so he should be an assistant and an assistant.

Of course, there are also advantages of being an assistant, he can follow Gu Qiang to learn some microscalpel operation methods, on this technology, if he learns Gu Qiang's eighty percent of the skills, he will not have to worry about eating and drinking for a lifetime - that is, in the 21st century, there are not many biotechnology service companies that can master this technology, and few are willing to use expensive cow embryos to train you.

The embryos divided by Gu Qiang will be directly sent to another group to do the steps of embryo freezing and thawing, although it is still the scope of experiments, but it also belongs to the steps of hand training, otherwise it will not be frozen one by one.

Compared with Gu Qiang's embryo segmentation, the operation difficulty of frozen embryos is much lower, mainly due to the configuration of reagents and the proportion of additives. The latter is their main training part, and the former is Yang Rui's work.

In order to receive money from the technical service company smoothly in the future, Yang Rui deliberately concealed some key details, such as the configuration of reagents, which he completed the whole process.

Compared with Gu Qiang's segmentation of embryos one by one, the workload of reagent configuration is much lower, and Yang Rui can configure a large number of solutions at one time, which can be used for a long time, with low cost and short time.

It only took a few days for the entire genetic engineering laboratory to enter the fluency mode, which is also due to Yang Rui's experience.

Up to now, he has been managing the ion channel laboratory and the Sinovel laboratory for two years, and the management experience he has accumulated is really not much inferior to other managers in China.

Some scholars in today's provincial laboratories may not have as much experience as him.

Of course, Yang Rui's fame is also a very important point.

For example, Comrade Ouyang Shi used to be known for his unruliness, and when he was at Shanda, he was even more excessive than Gu Qiang, but in the genetic engineering laboratory, he was willing to accept Yang Rui's trend.

Hearing Gu Qiang say that PCR technology is not difficult, he not only did not get angry, but also said very happily: "Good technology, mature technology, is to be simple, otherwise, how can the technology be promoted?"

"The technique of freehand segmentation is very simple, and Director Yang won't let me use it. Gu Qiang still remembers this, compared with the operation of the microscalpel, the freehand segmentation scheme is more elegant, but it is more comfortable.

Yang Rui didn't have anything to do under his hands, watching the two of them do things, and said calmly: "There is no potential to tap in the bare hand segmentation, the microscalpel plan is done well, how fast the success rate has risen." ”

"I don't have any potential to dig into, I think I can still improve. Gu Qiang said a little arrogant, his experimental level, not to mention the solitary step of learning, but it is no problem to walk alone in a few laboratories.

Yang Rui laughed twice.

Ouyang Shi was not polite, and immediately commented: "Boasting." ”

"Don't you believe it, let's try it?" Gu Qiang immediately put down the microscalpel.

"If the embryo is broken, the funds will be deducted. Ouyang Shi laughed and pinched Gu Qiang's seven inches.

Gu Qiang lowered his head helplessly, and could only operate while operating, and said, "I do two experiments at the same time, and I can do it too." ”

"What if you can do 10 experiments at the same time? If you can come up with a technique like PCR, that's the real thing. Ouyang Shi gave a thumbs up and said, "Do you know why PCR is eligible to win the Nobel Prize?"

"Oh?"

"Because PCR has promoted the development of biology and the development of human beings...... Ouyang Shi seems to be reading a textbook, but he is very pious on the outside, and he admires Yang Rui and even becomes a "fan uncle", which started from PCR.

On top of PCR technology, there are too many new technologies developed.

For example, the X chromosome associated enzyme assay and the Y chromosome specific DNA probe are at least 10 billion yuan if their social benefits are calculated. If you don't limit your expenses, you can support at least 10 girls with tattoos, smoking, drinking, and swearing, but you know you're a good girl.

And in terms of the derivative value of the entire PCR technology, there is no problem in feeding the runaway women of several group armies.

It can be said that PCR technology has opened a new chapter in biology, just as the microscope has opened the door to the microscopic world, and PCR technology has opened the door to the DNA era.

However, in the genetic engineering laboratory, Ouyang Shi is the only one who really has an international vision.

Ouyang Shi has not only participated in many international conferences, but he can also read English literature and publish English literature, which is common among middle and high-end scholars at Peking University, but if you look at it nationwide, it is very rare.

Therefore, in the entire laboratory, the most excited person is always Ouyang Shi.

He knew too well how powerful Yang Rui was.

For example, in PCR technology, most of the Chinese researchers at the level of technicians do not know what it is, and a small number of technicians with a certain level cannot really appreciate the power of it.

Whether it is Gu Qiang from Shandong University or researchers from universities in other places, although they know that the work they do in the genetic engineering laboratory in Haidian District is very good, they still stay in the idea of "filling in the gaps", and always feel that Yang Rui is imitating foreign technology, but cannot experience the profound changes in technology.

Now there are no online forums, no instant messaging software, and even no mailboxes, so everyone's sources of information and insights are limited to the people around them, and it is helpless.

Yang Rui didn't have time to talk about the contribution of PCR at this time, waved his hand, and said: "Don't brag, let's do the work first, and when the work is done well, the results will naturally be there." Professor Ouyang, your side is cooked. ”

"Oh, I forgot. Ouyang Shi said and took out the box of the PCR instrument.

Today's PCR instrument is the first version improved by Yang Rui, which is much simpler than the original version of PCR, and there is automatic power-off protection or something, which is basically equivalent to the work of cooking Malatang, which is simplified to cooking rice in a rice cooker.

Ouyang Shi paid a little attention and made two records, even if he completed a set of experiments and made records.

Gu Qiang was even more dismissive of this.

Yang Rui ignored him at this time, and instead focused on Ouyang Shi's experimental table.

The gender identification of embryos is the last big hurdle they face, and it is a big hurdle that they have not been exposed to before.

Whether it is an embryo transfer of mice or rabbits, Yang Rui does not arrange the part of gender identification, after all, if gender identification is not done, it will not affect the operation of the entire system.

However, when it comes to the commercialization of cattle embryo transfer, it is impossible to break the barrier of gender identification.

When doing experiments, no one cares how many male and female mice you have embryo transfer, but if Zhongmu spends money to do the embryo transfer of 1,000 cows, and the result is four or five hundred bulls, then they have to go crazy, what is the use of them asking for a bunch of bulls? They just want to slaughter and sell them abroad, and the number of four or five hundred is not enough to build channels, as for breeding, it is now 85 years, and of course the precious Simmental cattle must be artificially inseminated, and it is easy for a strong bull to breed millions.

Therefore, embryo segmentation, frozen transfer, and gender determination are equally important for the entire commercial embryo transfer system. Of course, techniques such as superovulation are also very important, but because there is no difficulty, they are not taken into account by Yang Rui at all.

The main obstacle to gender identification is the high requirement for early identification.

In fact, the identification of the sex of 8-cell embryos is only the minimum requirement for the commercialization of sex identification, and it takes up to 10 years to use Y-chromosome-specific DNA sequences to identify the sex of cattle embryos, with a minimum requirement of only 2 cells.

In other words, after the fertilized egg has multiplied once, it is time to do gender determination.

In this way, the cost of commercial embryo transfer is naturally reduced again, because it is the way of artificial insemination, the fertilized egg is determined, and the time of doubling into 2 cells is determined, so that after a round of identification, the embryos that do not meet the requirements can be quickly removed and started again with the least loss, thus greatly improving the efficiency of commercial embryo transfer.

If it is a large biotechnology service company, it can also directly take out embryos and distribute them according to the requirements of different customers, so that modern animal husbandry is under the control of industrialization from beginning to end.

The acquisition of this technical advantage makes gender identification an important technology that is not inferior to embryo segmentation and cryopreservation.

However, this technology is only new in the world.

There is no way, Yang Rui only came up with PCR technology for less than two years, and before the invention of PCR technology, whether it is cytogenetic analysis or differential analysis of embryonic development rate, the accuracy rate is relatively low, and the cost is relatively high, which is not popular with ranchers.

Correspondingly, after the advent of PCR technology, the gender identification before embryo transfer has become very popular, not only by ranchers, but also by biological companies, and correspondingly, the gender identification before embryo transfer has become a trade secret of competition between biological companies.

Techniques such as embryo segmentation and cryotransfer were developed around the 70s, and by the 85th century, even if the patent period had not expired, they were now widely used.

Gender identification is different, as the latest technology, PCR has promoted the research and development of a variety of new technologies, and these new technologies, whether they are patented or not, are naturally kept secret.

In this way, Yang Rui naturally could not use materials from abroad to explain his directional guidance, but had to overcome this technical difficulty step by step.

Original research is basically the same as complex research.

"It's a killer shot. Yang Rui comforted himself like this, and then threw himself into the information and data that was full of sweat.

……

(To be continued.) )