Sleepwalking Dafa is often heard in scientific stories, and most of them are touching the scene, plus the feelings of sleep. Pen & Fun & Pavilion www.biquge.info

For example, the discovery of benzene rings, the invention of PCR, and even the discovery of DNA double helix can be regarded as successful cases of sleepwalking Dafa.

The researcher dreamed back in the middle of the night, woke up from sleep, and solved the problem that he had been studying for a long time, this kind of epiphany model, in various scientific stories, is very popular, and ordinary people are also very popular, and one of his implicit messages is that ordinary people can actually make excellent scientific research results.

As a result, young people, middle-aged people, and the elderly who are interested in science will laugh at the sleepwalking stories of scientists.

Of course, ordinary people can't actually make excellent scientific research results.

Ordinary people can make some small inventions, add an eraser to the pencil and the like, there is no problem, but the coverage of basic science has exceeded the ordinary level, and there are many people who have not been able to get in touch with the world's cutting-edge science when they read a doctorate, not to mention the actual discovery process, and still have to follow the scientific process.

However, the second of sleepwalking Dafa is that it is a reasonable and legal reverse inference - the so-called I think the double helix is very handsome, so I tried it, it turns out that the double helix can really be, and it is the best guide to using the sleepwalking Dafa.

Yang Rui hopes to make a three-dimensional structure of G protein-coupled receptors, and the first step is to make a conformation of the transmembrane region of G protein-coupled receptors.

To put it mildly, such a conformation is worth half a Nobel Prize.

If you have enough basic biologists to make a conformation of the transmembrane region of the G protein-coupled receptor, it is almost enough to win the Nobel Prize, of course, it is right to share it with others.

It is such an important scientific achievement, but the conformation of the transmembrane region of the G protein-coupled receptor is a very simple image when it is displayed to the public.

Just like the DNA double helix, which is made of two snakes entwined, the conformation of the transmembrane region of the G protein-coupled receptor can be described as an image of seven springs stirred together.

The "molecule capable of transmembrane seven times" is a powerful protein molecule comparable to Shijiro in one night, and may be the most powerful molecule in the human body, but it is ultimately composed of seven tentacles, each of which is like a thick spring wire, and the image is really not good-looking.

However, such an image is not something that can be wanted.

The tentacle monster composed of seven tentacles is easy to draw, but the structure of the G protein-coupled receptor must stand up to scrutiny.

Yang Rui is going to use the Dafa of sleepwalking, not really preparing to sleepwalk, which is in line with the current cognition and judgment of the scientific community.

This is also what Yang Rui is rummaging through.

He had to see what he had to "sleepwalk" on.

The three intracellular rings and the three extracellular rings were figured out in the fifties and sixties, the first two intracellular rings were smaller, and the third intracellular ring was larger after a few years to be determined, and as far as the transmembrane helical lamellae of the receptor is concerned, helical I-IV forms one lamella, and helical V-VII forms another lamella, this understanding is relatively new.

In addition, the most important thing is the image data from the electron microscope and X-ray.

Although protein is such a small unit, the technology of the 80s was enough to capture a rough picture.

However, the transmembrane conformation of G protein-coupled receptors focuses on the word transmembrane.

What scholars want to figure out most is why G protein-coupled receptors can cross membranes, and they span seven membranes.

This is where the core problem of transmembrane conformation needs to be addressed.

Yang Rui was looking at the materials and making sketches on paper, which was diametrically opposed to what other scholars did.

Scholars of this era, studying the transmembrane conformation of G protein-coupled receptors from scratch, first had to imagine the placement of the seven antennae in their minds, then consider the relationship between them, and then examine whether they are consistent with the known structural information, and explain the parts that do not match......

This is a very tedious and delicate and even infuriating task.

The DNA double helix structure has only two strands, which is the lust of multiple laboratories, and the G protein-coupled receptor has 7 chains, so you can imagine the difficulty.

It's just that what Yang Rui has to do is not addition, but subtraction.

After 30 years, the transmembrane conformation of the G protein-coupled receptor is known, and the three-dimensional structure is known, not to mention the conformation of the first step.

However, Yang Rui can't directly take out the conformation of later generations, because of some constraints, the current academic community still doesn't know about it.

At the same time, Yang Rui also has to determine the research progress of his laboratory, and it is also necessary to deduce the conformational data from the research progress of the laboratory--it is said to be a drawing, but in the final analysis, all images are to be expressed in numbers.

And numbers, numbers naturally have to have a foundation.

Yang Rui turned all the information out, and barely made up seventy or eighty percent of the content, as for the rest...... Naturally, you have to force others to do it.

"When I went to bed last night, I thought about it carefully, the handover of the 7 spiral chains must have a certain effect, and I tend to use them to identify substrates, and they are specific to identify substrate molecules......" Yang Rui said while writing two lines on the blackboard in the laboratory:

1. Determine the contact between the 7 helices and the substrate molecules.

2. Describe the local conformation according to the substrate molecule.

A simple twenty or thirty words is like a container smashed on a jeep, and everyone is out of breath.

"When you sleep, you think about these things?" Gu Qiang looked at the two questions and wanted to complain.

Yang Rui nodded steadily, sleepwalking Dafa is good, but the good thing is that the idea is simple to explain, avoiding a series of logical problems.

Gu Qiang was very helpless, pouted, and said, "I wonder how you can sleep." ”

Yang Rui coughed twice and said, "If you give an answer, I will definitely be able to sleep." ”

"There's no ready-made solution, is there?" asked Professor Manhai.

"Nope. ”

"So, it's all about trying?"

Yang Rui shrugged.

In order to measure the contact between the 7 helices and the substrate molecule, it is natural to test the substrate molecule and the G protein-coupled receptor, and the substrate molecule is hundreds or thousands, and the test is naturally tens of thousands.

Professor Man Hai only felt that his eyes were dark, and muttered, "No wonder you asked them to make so much bovine viptoplasmin." ”

"It's still our advantage period, everyone refuel, and when the project is over, everyone can go abroad." Although Academician Zhu came forward to do the work, the trip abroad was vetoed, which was still very demoralizing, and Yang Rui could only take the opportunity to draw cakes.

Man Hai also took the opportunity to show his loyalty, saying: "As long as you can do it, it doesn't matter if you go abroad or not." ”

"Making results is the focus now. Yang Rui quickly pulled the topic over and said, "I now have some preliminary ideas about the conformation of G protein-coupled receptors, and I hope you can do your own work and contribute to the completion of the project......

Yang Rui didn't take out his sleepwalking flow composition as a whole, but he still made a few simple partial drawings and handed them to several researchers.

A group of people studied silently, some couldn't understand, stared blankly, and some understood, and began to look for old materials for comparison.

Yang Rui didn't care, but felt a little drunk after drinking.