.

The positive contact of the Americans, especially Dalbeko, gave great stimulation to the five groups including Zhang Leshan. Pen Fun Pavilion wWw. ｂiquge。 ｉｎfo

Zhang Leshan and others, in order to welcome the arrival of the Americans, did not hesitate to increase the funding for PCR-related experiments.

For example, Director Zhao's heat-resistant polymerase group even increased expenses and manpower in order to improve speed.

Every time Dean Zha came out of the office to inspect and see the vitality of the newly established laboratories under him, he was deeply gratified, and he only felt that the entire scientific research system seemed to be brought to life by himself.

Director Zhao and others also feel very cool, compared to doing research by themselves, repeating experiments or reverse engineering always makes people feel more confident.

Innovation is like a deep mountain and old forest that no one has ever been to, you get into it, you may not get anything, the only thing that is certain is that you have to work hard to get out, and there is even the possibility that you can never drill out again.

It's as simple as repeating experiments or reverse engineering, it's like going to a famous mountain and river, you get in, you can basically determine what you can get, the only thing you need to do is to work hard, a little hard, maybe you have to give some more tickets, that's all.

Compared with the deep mountains and old forests, it is naturally more comfortable to develop the famous mountains and rivers developed by the predecessors, and it would be even better if you could save tickets and travel expenses.

The refreshment of Director Zhao and others lasted for at least half a month, and by mid-February, Director Zhao's own heat-resistant polymerase group had problems first.

"According to the literature, the polymerase can only withstand high temperatures up to 45 degrees, and it can no longer be improved. Zhu Guanghui, the researcher in charge of the experiment, reported to Director Zhao anxiously.

"Didn't you do it again?" Director Zhao, who had just returned from Donglaishun, was full of oil, and he didn't care about the taste.

"I redid it and re-isolated the strain, but once I made it, the high temperature resistance was ...... Improved by two degrees. Zhu Guanghui said quickly and urgently, and when he talked about the specific temperature, he lowered his speed of speech, and he was also anxious.

Director Zhao frowned, and after a while, he said, "45 degrees, it's too much worse." ”

Zhu Guanghui blushed a little, and then straightened his chest again and explained: "I know that 45 degrees is a little lower, but compared to the previous research on heat-resistant bacilli, this temperature has increased a lot. ”

"Before Yang Rui made heat-resistant polymerase, the best heat-resistant polymerase could not reach 40 degrees, but now we have increased it by at least 20%. Another researcher next to him also spoke.

"Yang Rui has achieved heat-resistant polymerase above 90 degrees. Director Zhao only felt that the teeth were tight, as if there were shreds of flesh stuffed into them.

Zhu Guanghui's face turned red, he was afraid that Director Zhao would say this before, but now he finally heard it.

"In a little while, we should be able to improve. Zhu Guanghui's voice was like roasting meat, a low and thin sizzling sound.

"How much?" asked Director Zhao.

"50 degrees should be fine. ”

"50 degrees doesn't make any sense. "Heat-resistant polymerase is the raw material when the PCR instrument works, and it must be able to withstand high temperatures of 96 degrees, and polymerases below this temperature are just ordinary polymerases.

If there is no heat-resistant polymerase, it is necessary to go back to the era of manual PCR and do DNA replication like making Malatang.

It can be said that heat-resistant polymerase is an important part of the PCR instrument, and it can even be said that it is with heat-resistant polymerase that there is a generation of PCR instrument.

Director Zhao stared at Zhu Guanghui with serious eyes.

Zhu Guanghui stumbled and said, "We still need time." ”

"We have time, how long do you need?" Director Zhao's expression did not soften, he knew that there must still be problems, otherwise, the atmosphere in the laboratory would not be so good in the first month and a half, especially after the official start.

Zhu Guanghui really said: "We have found several situations that we didn't expect before, and now, before breaking through the difficulties, we can't determine the specific time. ”

"What can you do if you give you another month?"

Zhu Guanghui whispered: "One month is not enough." ”

"A month and a half?"

Zhu Guanghui shook his head slightly.

"Two months?"

Zhu Guanghui's head hung down.

"You name a number. ”

Zhu Guanghui thought for a long time and said, "Half a year ......"

"Can you make the same heat-resistant polymerase in half a year?"

"Maybe we can make a thermostable polymerase that works. Zhu Guanghui's voice was a little lower.

Can be used and the same are two concepts. As an enzyme, heat-resistant polymerase actually has the best working temperature, and the so-called heat-resistant usually refers to the fact that it can also play a role under high temperature conditions, which is the so-called use, but it is very difficult to exert the best performance under high temperature conditions.

Yang Rui has done sufficient preparation, especially with a clear direction, in order to make a good polymerase.

But for Director Zhao's experimental group, this problem is not so simple.

However, as the most important raw material of the PCR instrument, whether the heat-resistant polymerase is good or not will greatly affect the effect of the final PCR instrument.

Director Zhao said in a deep voice: "Just maybe, two days ago, you were still very confident." ”

"We didn't expect that as the temperature increased, the problems we encountered also increased exponentially. Zhu Guanghui faced the gloom.

Director Zhao darkened his face and said, "Show me." ”

Zhu Guanghui hurriedly took out the experimental records, and then explained to Director Zhao a few key points encountered.

Director Zhao is the person in charge of the laboratory, and the level of scientific research is not weak.

After looking at it for a while, Director Zhao found that Zhu Guanghui's judgment was not wrong.

"That's the problem. Director Zhao shook the experimental records, and all he thought about was money and time.

Although it is reverse engineering and there are literature references, the specific experimental procedures, materials and details are not clear, and there are still countless possibilities for crossing.

Director Zhao's laboratory is inferior to Sinovel in terms of conditions, not as good as Sinovel in terms of funds, and inferior to Sinovel in terms of direction and guidance, and the time they invested is far from the time invested by Sinovel and cannot be done.

Similarly, if the research on potassium ion channels is to climb Mount Hua, the heat-resistant polymerase is about equivalent to climbing the Jade Dragon Snow Mountain, which is not as difficult as Mount Hua, but it is the same hardship and danger.

In such a tourist area, if you are well prepared, people in good health can climb it, and the difference is nothing more than time and energy investment.

Yang Rui spent half a year preparing and half a year to do it, and Huang Mao, who actually operated, was even more of a calve-level figure - his current level naturally did not reach Maverick, but his experimental ability was already comparable to Maverick.

Director Zhao underestimated, and only when Mount Tai dealt with it, he would naturally misestimate the time and cause delays.

"Director, what now?" said a researcher in the lab who asked the question everyone wanted to ask.

In a laboratory, the biggest and most important person in charge usually does not have to do the experiments himself, just like the biggest and most powerful executive chef in a big hotel, usually not in charge.

The person in charge of the laboratory, in addition to obtaining projects and funding, is mainly to determine the direction and solve the problem, just like the executive chef, who mainly decides how to make a dish and what to do if there is a problem.

On top of that, he naturally has to oversee time and budget, just like an executive chef has to tighten the pace of food and limit the use of raw materials.

Usually, the director of the laboratory can go to eat Donglai Shun if he wants to, but when the problem arises, he has to make a decision.

Director Zhao is full of mush.

Heat-resistant polymerase has not been made in China before.

Before PCR, this was originally a relatively important branch of scientific research.

Therefore, from the point of view of scientific research, from scratch to make a heat-resistant polymerase, and the temperature of the heat-resistant polymerase increased to more than 40 degrees, this is a very good achievement, enough to fill the domestic gap - wait, the domestic gap has been filled by Yang Rui, which is probably one of the reasons why some people don't like Yang Rui, in the past, foreign countries to make an innovative achievement, domestic copy, this is to fill the gap, now, Yang Rui has made innovative results, domestic copy, what use.

Therefore, before the scientific research community reinvents a word, the classic expression of filling the domestic gap will become difficult to use.

As far as heat-resistant polymerase is concerned, Yang Rui actually made high-temperature polymerase raw materials several years in advance, but the capital he invested in this was not available to Director Zhao.

"I didn't expect it......," Director Zhao fell into deep thought.

"I didn't expect it, ah......" Several scientific research groups fell into contemplation one after another.