Yang Rui slept in the ion channel laboratory for several days. Pen "Fun" Pavilion www.biquge.info

It's not that he wants to overdraw his physical strength to fight from the beginning, but Comrade Su Xiankai, Fan Zhenlong and Wang Sisheng are high-spirited, and he has to reluctantly make it difficult and work overtime.

In fact, if he enters the middle of the experiment, the overtime of the three of them may not necessarily affect him, and the scientific ape is tired and tired, and the keeper travels around the world to give speeches too much. However, the early stage of the experiment was different, and there were so many things that Yang Rui needed to confirm, so that the three of them had so many questions that Yang Rui couldn't hide.

The work of the project leader is like this, if there is no problem with the big cows and calves under his hands, and the dogs are all working step by step, then he can also work step by step, and can even take a vacation step by step, just like the rancher can enjoy the fresh air, the delicious steak, and the plump cowgirl or cowboy or sheep in the wind and rain, cowboy power, and nothing around him.

However, the wind and rain are smooth, the cowboy is powerful, and there is nothing to do around, which is too rare.

Especially a newly built ranch is simply a swarming affair, and just confirming the purchase of reagents has been busy for Yang Rui for an afternoon.

If the run-in of the G protein-coupled receptor group is smooth, like buying reagents or something, Yang Rui can not ask at all, it is just a regular laboratory life, just like buying what brand of gloves and what type of paper to draw paper with.

But at the beginning stage, Yang Rui really couldn't ignore these small problems.

The simplest reagent purchase is also compatible, such as buying domestic reagents, or imported reagents, it seems to be easy to say, if you have money, you will buy imports, and if you don't have money, you will buy domestic ones.

For a research dog who will graduate in two or three years, the answer may be true, but from the perspective of the project leader, the problem is completely different.

What Yang Rui needs to determine at the beginning of the stage is actually the reagent needed for the entire experimental cycle. Otherwise, if you use imported reagents in the early stage and domestic reagents in the later stage, and then compare the two, the result curve will definitely be messy. In this kind of high-standard international scientific research competition, the articles are all aimed at s, and the figures in them are to be read repeatedly and even repeated experiments, and messing up will ruin a good data.

Moreover, the purchase of reagents is not limited to the difference between imported and domestic, Yang Rui's final answer on paper is not only to decide which country, but also to decide which factory of which company, and even a certain batch.

The purity of the reagent has excellent purity, analytical purity, chemical purity, chromatographic purity and so on, respectively, corresponding to the number of impurities, but for some reagents, no matter how high the standard, it is still impossible to completely eliminate impurities, for people who do experiments, this is a very annoying thing, you don't have the conditions, let me do the experiment?

Unfortunately, it does not mean that the actual conditions do not have the ability to talk about scientific research, the correct way is to ensure the consistency of impurities, the impurities of the same factory and the same batch of reagents, can usually be guaranteed to be consistent, so that through comparison and calculation, the influence of impurities can be eliminated to a large extent.

The same approach can be extended to draw two conclusions. First, even if it is a high-purity experimental reagent, it is better to buy a batch, because you don't know if the other party's factory will produce a batch of unqualified reagents, so you will encounter it. Putting your years and millions of dollars in the prudence and professionalism of others is imprudent and unprofessional.

Not to mention, scientific research is inherently doing things with small probabilities.

The second conclusion is that it is possible to use a lower purity reagent to do a relatively more demanding study, as long as the data is processed in the later stage, which is a completely regular process, and the result is similar to that of an experiment with a medium and high purity reagent. Chinese labs have always done this, and the main reasons are...... Because of poverty, of course.

In addition, the purer the reagent, the better.

The first problem is naturally whether the purse can stand it, if the purse can bear it - in extreme cases, in fact, there are examples of not buying high-purity reagents, such as those who make materials, they often like to buy domestic reagents, because domestic reagents often have inexplicable impurities, and occasionally they can bring surprises to people.

There are many examples of so-called impurities in the history of science, and they became popular at the end of 16 and the beginning of 17 years.

Secondly, the purse is not to buy reagents and look at the expensive ones.

The concentrated sulfuric acid used to dispose of the waste liquid, you can't buy the pure one, even if you are rich in oil, it is a little too hard to dismantle the small packaging bottle of the pure liquid and pour it into the waste liquid barrel.

Totally thankless.

Besides, there is no one who is really rich in scientific research on the earth, just take the biological laboratory in 86 as an example, a gram of 50 million US dollars of interferon, you just say you want a few tons.

G protein-coupled receptor is originally a money-burning project, and the start of $6 million is enough, but it must not be wasted at will, especially in the initial stage, and the establishment of standards should be more careful.

You can't just because the fog of war has opened and you have a dual base right away.

If the project is to be done for three or five years, or even five or eight years, it is impossible to say that when it comes to the fourth and seventh years, it is impossible to suddenly switch to cheap reagents, and the value of the previous experimental data will be greatly reduced.

This is actually a challenge for Yang Rui.

The world is changing too fast, to some extent, there must be some predictions, this kind of prediction, not only to predict the time of their own projects, the expected funding, but also to predict the state of the opponent, the trend of the scientific research community.

Of course, the most important thing is experience.

If it is in developed countries such as Europe and the United States, Yang Rui can also try to consult scholars from other top laboratories, but unfortunately, there are no corresponding conditions in China.

Professor Cai and others, although they have made world-class results, they have not set up an international-level laboratory, and have obtained world-level results under the condition of carrying out world-level projects.

In summary, since the artificial synthesis of bovine insulin in China, there has been no such targeted large-scale biological research and development. In the past 20 or 30 years, there has been basically no scientific research in biology that has been prepared for war and then participated in a positive scientific research competition at the international level. To put it all on, it's almost all ambush warfare, special warfare, or behind enemy lines.

Back then, when the Institute of Biochemistry of the Chinese Academy of Sciences, the Department of Chemistry of Peking University and the Institute of Organic Chemistry of the Chinese Academy of Sciences gathered heavy troops, organized the distribution of jackets, led the pre-war mobilization, and opened a scalpel to artificially synthesize bovine insulin. However, one after another, although it is only an American laboratory and a German laboratory that are fighting against each other with the strength of the whole country, in a specific field of scientific research, this is indeed the highest level of confrontation, and it may not be possible to shock the world, but it is indeed possible to shock China.

However, that was 30 years ago.

Therefore, Yang Rui had nowhere to ask for advice.

However, on the bright side, this also makes Yang Rui have no competitors and less expert guidance.

"Anyway, it's my own money, and I pay for my own tuit......ion," Yang Rui gritted his teeth and signed various documents and projects.

The dollar account deposited in the People's Bank of China is also a slippery decline, and the distressed branch business managers call to inquire.

$100,000.

$200,000.

$500,000.

$1,000,000.

$2 million.

By the time the preparations were completed and the instruments and consumables to be used in the early stage were ordered, the $2.7 million had already been out.

Looking at the figures released inside the laboratory, Su Xiankai was bored at that time, and in an instant, there was a cold sweat on his back.

"Good thing you didn't listen to me at the time. Su Xiankai is from beginning to end, knowing that Yang Rui did not choose particularly expensive products, he basically looked for them in the lower middle range, which belongs to a more frugal opening mode, not that he can't be thrifty anymore, but if he is thrifty, he can only do tracking experiments and is not qualified to participate in scientific research competition.

However, with such a standard, it turned out to be 2.7 million US dollars, almost three times the figure that Su Xiankai wanted, and Su Xiankai was also afraid for a while.

There is no turning back from the start of construction, $1 million has been spent, and if you don't see a sound, you won't be able to get it back.

Yang Rui didn't feel happy, but said: "Needless to say, once you succeed, the remaining funds can euphoreize people to death, maybe you can leave one or two seven-figure figures......

"Director Yang, don't provoke us, we haven't even found the door. Fan Zhenlong couldn't laugh anymore, recently just a purified G protein made him and Wang Sisheng want to die, not to mention the whole set of experiments.

Su Xiankai, a skilled worker who has already purified a group of G proteins, did not have any confidence, and said: "If you can achieve rhodopsin protein, then if you have a successful time, I already feel a little bit on my side, but your requirements must not be so low." ”

Yang Rui rolled his eyelids and said, "Bovine rhodopsin is an experimental material, and it hasn't entered the experimental process yet, Lao Fan, what about your side?

The use of detergents can increase the solubility of G protein, which is currently the best means to purify G protein.

But Fan Zhenlong still shook his head and said, "It's easier to use, but we haven't found the trick to extract the receptor from the cell membrane." Lao Su couldn't figure it out. ”

Yang Rui naturally looked at Su Xiankai.

Su Xiankai was stunned and said: "Lao Fan, the one they chose to purify is GT, and I haven't touched it, so I can't grasp the various doses again." I don't actually have any tricks, I just do a lot of it, and it takes a year to purify it. ”

There are many types of G protein and G protein-coupled receptors, and there must be a certain amount in order to make a systematic comparison, so although purification is for Fan Zhenlong and others to practice, it is also one of the necessary conditions in the early stage of the experiment.

But if you want to wait another year and a half for one more purified protein, the laboratory can't afford to wait.

Yang Rui couldn't help but bow his head and ponder.

The secret of purifying G protein with detergent is also what he sees from the book, and he has not operated it in the field, and he can't talk about specific experimental guidance in terms of his slag experimental level.

In any case, the experimental level of Fan Zhenlong and other calves is also stronger than him, however, they can become big bulls in the future, but not by relying on the experimental level, if the experimental level alone, the three of them are afraid that they will not be able to play with Gu Qiang.

Thinking of this, Yang Rui couldn't help patting his head and said, "In this way, I will add another farmer to you...... No, add an experimental assistant, it's Gu Qiang from the genetic engineering laboratory, who has strong hands-on ability, you can cooperate with each other. ”

Learning also needs to be helped, since the speed of idle mining is slow, then add a farmer.

Anyway, the genetic engineering laboratory is also under his name, and they are all public laboratories, so it is still very convenient to borrow them or something.

……