When Yang Rui described it to Professor Jiang and Academician Zhu, he understated it, but in fact, he knew that the use of bovine rhodopsin for the study of G protein-coupled receptors was at least three S papers away from the current mainstream scientific research direction. Pen @ fun @ pavilion wWw. ｂｉｑUｇE。 ｉｎｆｏ

The first top paper is to sequence the adrenergic receptor gene, the second is to sequence the rhodopsin of cattle, and the third is to find that the amino acid sequence of the adrenergic receptor gene and the G protein-coupled receptor has a high degree of similarity.

This whole process actually has a certain degree of probability.

What if Kobilka didn't do the sequencing of the genes that the adrenal glands can receive? What if no one happened to do the sequencing of bovine rhodopsin? What if no one was idle and compared the amino acid sequences of the two?

It's really all a layman's problem.

These three probabilistic questions are like asking, what if no one captures the hills in front of you in a siege battle with heavy troops, what if it doesn't happen to destroy the enemy's heavy artillery group, what if it doesn't happen to coincide with the time......

G protein-coupled receptors are fighting a meat grinder, and there is not so much why, Kobilka didn't do the sequencing of the adrenal receptor gene? It didn't take long for someone to do it.

Maybe a year or two later, but not too long.

What if no one goes to do the sequencing of bovine rhodopsin? It's like asking what to do if three hours of artillery fire is not enough, and if it is not enough, it will be blown up for another three hours!

So many laboratories, so much money was spent, and in the end they all worked in vain, and they didn't even have a name left, and doing a laboratory with adrenergic genes and bovine rhodopsin was just choosing the right goal and making results.

There are many soldiers who charge, and there are always those who survive, not because the enemy's machine gun is slow, not because you have special skills to dodge guns, but because there are enough people on your side to block the gun holes, and your dog's life is big enough.

Of course, Yang Rui is not ready to be cannon fodder.

That's why he sacrificed bovine rhodopsin protein.

This is a secret that Europe and the United States have only recently discovered.

It's a scholar who reads the literature relatively quickly, and that's about what he saw.

If Yang Rui doesn't look for it from time to time, I'm afraid that he may not be able to see this information in a year or two.

In a year or two, I have been very nervous about other scientific research projects, and in terms of G protein-coupled receptors, it is almost equivalent to giving up completely.

This is also a part of the difficulty for scholars in third world countries to do scientific research, if it is in the United States or Europe, especially in a region where a project is done well, the flow of information is much better, and there are more exchanges between scholars, although it is not updated in real time, it will not be too slow after all.

Especially compared with laboratories in backward regions and countries, the difference in time is very decisive.

If another domestic laboratory finds itself two or a year behind the international mainstream scientific research results, will it still participate in such fierce scientific research competition? Most of them will choose to retreat.

After all, funds are limited and cannot be wasted.

Also closely related to funding is the timing and timing of the publication.

In terms of national and regional prejudices, European laboratories are the most capable laboratories that can hold back their achievements, and there are many laboratories that have sharpened their swords in ten years, and then Japanese laboratories are more able to hold back, because they have a tendency to exchange domestically, and the independence and authority of scientific researchers can be relatively guaranteed, so it is not too much trouble to hold back for three or five or even seven or eight years.

In comparison, American laboratories are not so able to hold back, and sometimes, for the sake of funding, laboratories are eager to publish results.

For example, the relationship between rhodopsin and adrenergic genes in cattle may be a year or two ahead of other labs if they are not published—or a month or two, of course.

It also depends on the requirements of the laboratory for the results.

If the goal is only to publish S-level papers, then the relationship between the two is sufficient, and the goal is achieved, so it is natural to publish the paper without hesitation.

But if the goal is a Nobel Prize-level achievement, then even if it is a risk, it will have to wait for a while to be published - this is usually the advantage of more funding, wide sources, and fewer restrictions.

In reality, the publication of the relationship between bovine rhodopsin protein and adrenal receptor genes also represents a new field in the study of G protein-coupled receptors.

Most of the laboratories are once again on the same starting line.

The cruelty of the meat grinder-like scientific research competition is nothing more than this. No matter how hard you've worked before, if one person publishes the results before you, everyone will have to start all over again.

However, Su Xiankai and others have obviously not yet felt the cruelty of scientific research competition, especially the cruelty of the world's top scientific research competition.

Several of them looked at the literature with a smile, and by the way, throwing Yang Rui two little farts was counted as encouragement.

Including the eldest Xu Zhengping, who also looked at the three with an optimistic smile, he was not ready to participate in this project, but it did not prevent him from looking forward to the project.

Only when the ion channel laboratory is well developed, the group in the laboratory can live well, which has long been proven.

Yang Rui will not hit them, let them be happy for a while, and then said: "Now that the goal has been clear, we will follow the direction that our predecessors have opened up, and the first step is to extract bovine rhodopsin protein." Who's going to do it?"

The three of them looked at each other, and Su Xiankai said, "I'll be a striker." ”

"Then I'll leave it to you, I want a lot of bovine rhodopsin, and it must be purified cleanly. ”

"Leave it to me. Although Su Xiankai was a little pressured, he still agreed. There is no 100% guarantee for this thing, so I can only say that I will do my best.

Yang Rui also confirmed the start of the project.

As the project leader, his instructions can be reached at this step, and how to extract bovine rhodopsin protein after that needs to be considered by Su Xiankai himself.

This is like a siege battle, Yang Rui wants to capture a fortress in the forward position, he does not need to describe in detail the tactics of attacking and killing the fortress, it is the responsibility of the front-line commander.

Su Xiankai is the current front-line commander, and he doesn't have to charge independently, the scientific research dogs in the selected room are all used to charge him. Only when it comes to the difficult point, the key point, or the front is really impossible to attack, does Su Xiankai himself come forward.

Regarding the work of Fan Zhenlong and Wang Sisheng, Yang Rui thought about it for a while, and said: "You can accompany me to read the literature now, and then do a few sets of repeated experiments, and choose a few G proteins for purification, GT, GS, GI and the like." ”

Fan Zhenlong and Wang Sisheng opened their mouths, but in the end they could only nod helplessly, put their ambitions in their hearts, and started by practicing basic skills.

The Ion Channel Laboratory of Peking University is still not a world-class laboratory, not only because of the lack of funds and equipment, but also because of the lack of accumulated scientific research ape foundation.

Fan Zhenlong and Wang Sisheng had not done work such as G protein purification before, so they were equivalent to front-line commanders who had not carried out siege warfare on this battlefield, and they had no choice but to spend precious funds and time.

In fact, Yang Rui himself has not done G protein purification, and the complexity of this work has exceeded the scope of master's students or ordinary doctoral students, but Yang Rui has now transcended the role of front-line commander, and in the position of campaign commander, Yang Rui is already experienced.

Even if it is a meat grinding battle, Yang Rui is not false at all.

6 million US dollars, three active calves with the potential of big cattle, and three calves as reserves have not yet been used, that is, pure resources, Yang Rui feels that he also has the opportunity to fight a Taierzhuang.

All bloody battles, fought to the end, are contested with perseverance and willpower, and when you think about it, the team that can see the light of day always has to take advantage of it.