"So, Director Yang decided from the beginning that he wanted to breed this sheep asexually?"

"It seems so. ”

"Then we will also do an experiment on asexual reproduction in the future?" Su Fan's tone became more and more solemn.

Wang Liangcai nodded solemnly in agreement and said, "It seems to be so." ”

"Are you kidding? shouldn't you...... At the very least, we should do a successful clone of sexual reproduction first, and then do asexual reproduction, right?" Su Fan's thinking was about to be confused.

Before that, when everyone talked about cloning, they were talking about sexual reproduction cloning, which is to put the nucleus of a germ cell into a nucleated egg cell.

So, cloning is not a new word.

However, even so, cloning is still a big project in genetic biology, such as the famous childrenfish, which is typical of this type.

In the development process of cloning, it basically follows the process of amphibians to aquatic products, then from aquatic products to mammals, and then from mammals to economic animals.

Su Fan and Wang Liangcai both felt that it was time to clone into an economic animal, and they had a kind of self-confidence and high-spiritedness of the terminator.

However, the sudden news of "asexual reproduction" blinded both of them.

This is equivalent to crossing several steps, like a child who has just started to play outdoors, and goes directly to Mount Everest, and does not give him a helicopter.

Most importantly, biologists have not been able to prove that asexual reproduction is really feasible without similar experiments conducted by previous researchers.

The normal model, now with an easier organism, first proving that asexual reproduction is feasible and then doing mammals, is the simplest and easiest solution.

But, to put it another way, what creature is easier?

Microorganisms don't need to be considered, even larger fish and amphibians? their sex is very vague in the first place, and the situation of female fish becoming male fish often happens in nature.

So-called, if you want to talk about sexually stable creatures, you have to at least have mammals.

So, would it be easier to clone small mammals, such as mice and rats, which are most common in the lab?

Not really!

Wang Liangcai, who had been experimenting for a while, came to this conclusion with a turn of his mind.

It takes a certain amount of time to carry out enucleation and nucleation, and the embryonic development time of mice is only two weeks, and the embryo development time of sheep is six weeks, and the latter is not known how many times easier than the former.

In this respect, animals commonly used in laboratories are not suitable for cloning. Because one of the reasons laboratories choose them is because they grow and develop quickly.

This is a good choice for experiments with adults, but when the subjects are embryos, these animals commonly used in the laboratory are not suitable.

Wang Liangcai raised his head and peeked at Yang Rui, and said in a low voice: "Director Yang preferred sheep as donors, and it was a one-time idea." ”

Su Fan is also a shrewd person, and after thinking about it, he knew why Wang Liangcai said this, and said helplessly: "Director Yang is not a person who likes to do projects step by step. ”

Looking back at Yang Rui's previous projects, from the experiments on potassium ion channels to the G protein-coupled receptor project, none of them were really done step by step.

Su Fan and the others didn't know what Yang Rui really relied on, and when they recalled it at this time, they could only evaluate it like this.

The so-called successful people are always successful cases, and Wang Liangcai has no way to criticize them, so he can only sigh and think, young people just can't hold back their temperament.

However, such an idea can only turn in Wang Liangcai's mind. As far as Yang Rui's current reputation is concerned, it has long been disassociated with age, and people don't like to follow the rules, that is, forge ahead......

"What now?" Su Fan is not a particularly thoughtful person, so he still asked Wang Liangcai first.

"I've never done a clone of asexual reproduction, and if it does, the success rate is extremely low. Wang Liangcai sighed.

The success rate is an eternal pain in the hearts of experimental biologists, and many experiments are done knowing that they can be done, and then the success rate is outrageously low, and finally abandoned.

Now when you encounter experiments that may not be able to be done, and the success rate is as low as the outrageous, the result is disgusting - you don't know if your failure is a mistake or if you can't make it.

At this time, Wang Liangcai was extremely eager to have a result prompt board to tell himself which way was doomed.

"We need to improve our early success rate. The previous plan had to be reversed and restarted. Wang Liangcai quickly made a decision, and said: "The success rate of denuclearization should be increased to at least 80%, and then the nuclear injection should be done." ”

Su Fan was not surprised that Wang Liang made this plan.

The step-by-step academic school is like this, and it is impossible to fight for luck like Gu Qiang.

Stacking success rates on small, simple, and inexpensive projects with a last-ditch shot is the most common.

No one is going to turn a bicycle into a motorcycle, and the academic thinking is that it would be good to be able to change to a good bicycle, and besides, those who engage in academics don't ride motorcycles, right?

Su Fan didn't mean to dissuade him, so he held a learning attitude and said, "If you want to improve the success rate in the early stage, do you want to do a comparative test?"

"Yes, to do a comparative test, it is better to find a few more people. Wang Liangcai said, his eyes wandering around the laboratory.

Doing a comparative test is actually doing a small experiment.

For example, whether the operating solution is sucrose with or without sucrose, and whether 2% sucrose or 3% sucrose is used, all of which are small details that cannot be smaller. However, the success rate of the experiment is such a small detail that is piled up.

Why is it the same egg peeling, some people's success rate can be as high as 70 or 80 percent, and some have a success rate of only 20 or 30 percent, in addition to the difference in technique, it depends on who notices more details, and who knows more details.

Doing comparative experiments is to measure the small details.

If you change to a local university, you can do such a comparative experiment and publish a paper.

A large number of Chinese journals and SCI entry-level journals publish papers of a similar level. In fact, Yang Rui's first paper, the ultraviolet spectrophotometry of coenzyme Q10, is one such article, which solves a single problem in a narrow direction of related fields, so it can only be published in a very low-level journal and become a material that scholars with relevant needs can find.

Computer retrieval, or in other words, before the emergence of academic search engines, such data retrieval also required a lot of manpower and material resources.

Su Fan didn't need Wang Liangcai to assign tasks, and said first: "I'll go to the data team to urge it, and put the direction of the search in the early stage?"

"Yes. Wang Liangcai's gaze was still roaming the crowd.

Because of the new construction of Yang Rui's genetic engineering laboratory, the system adopted by Yang Rui is different from that of the ion channel laboratory, which adopts the system of a large laboratory, allowing researchers to join and form research groups freely, so it is more competitive.

However, the establishment of a research group requires funds and time, and of course, it is necessary to have a research project.

The 600,000 + 30,000 US dollars that Gu Qiang won from Yang Rui, as well as the machine time of the inverted phase contrast microscope, all belong to the resources of the research group.

Although the research group is still under the constraints and guidance of Yang Rui, compared with the old model of ion channel laboratories, the large laboratory system of genetic engineering laboratories is obviously more independent and more suitable for larger and larger laboratories.

Theoretically, Wang Liangcai, who is seconded, can bring in people as long as he obtains the consent of other researchers, and anyway, it would be good to include the name in the paper according to the contribution. This is where the main interest of the laboratory lies.

"In the old Haidian District Genetic Engineering Laboratory, researcher Sun Zhengchu has the strongest level and good cooperation ability. Su Fan introduced Wang Liangcai in a low voice, turned his head and said: "In terms of experimental assistants, I think Qin Yue is good, he used to be a veterinarian in Zhongmu, and he has been seconded to the genetic engineering laboratory in Haidian District for a long time. ”

"This Qin Yue ...... If it's just an experimental assistant, isn't it a bit too cumbersome to transfer it? Wang Liangcai hesitated.

Su Fan said with a big grin: "I have the right not to use it, it will be invalid when it expires, we are now in the national project team, do you think about how the previous projects of the same level were carried out? ”

Wang Liangcai was dumbfounded. He had been doing experiments at university before, and he had never been involved in a project of this scale.

In biology, there are really not many so-called big projects, they are all in medicine-related fields, genetic engineering has always been niche, and Wang Liangcai really hasn't tried to scold Fang Xuan.

"There must not be many experimental assistants, and if there is anything to say, it is scarcer than researchers. Su Fan paused and said: "A good experimental assistant is not easy to find, Qin Yue's words, he has experience in veterinarians, knows things in the laboratory, and has also participated in the project of embryo transfer of cattle, he is experienced and hard-working, which is very appropriate." ”

Wang Liangcai has students under his hands, but it is not appropriate to pull people from afar, and experimental assistants are necessary.

As Su Fan said, a good experimental assistant is still indispensable, sometimes, an unwashed test tube may lead to the failure of the experiment, and a good researcher may not necessarily wash the clean test tube.

"Just as you say. Seconded Qin Yue to come over. After Wang Liangcai finalized it, he was in a good mood, and finally clenched his fists like the characters on TV, and said, "Let's have a big fight." ”

"Go for it. Su Fan laughed.

As the two laughed, the lab became noisy.

Wang Liangcai simply stopped and said with a smile: "Just go over and see how the others are doing." ”

"Good. Su Fan followed Wang Liangcai through the public area where many instruments were displayed, and saw Gu Qiang being surrounded by a group of people.

Su Fan instinctively frowned, and asked Tian Bing next to him, "What kind of moth is he making trouble?"

Tian Bing said with a sigh: "You would never have imagined that Gu Qiang has never failed to remove the nucleus from the start of construction to the present, and he has just done the nucleus absorption of breast cells, and now he is ready to inject the nucleus." ”

"You haven't failed a single step...... Su Fan actually wanted to say that there was no failure in a single step, and there was nothing remarkable, because as long as you failed one step later, the previous efforts would be wasted. So, that's why they're constantly trying to improve their success rate.

However, when the first half of this sentence came out, Su Fan couldn't say the second half of the sentence.

In any case, Gu Qiang's current progress is the first in the laboratory.

"If his injection is successful, this will be the first successful case of nuclear injection in our laboratory. "Tian Bing is here to help, purely to see the lively mentality.

Su Fan laughed awkwardly twice, and was about to say something, but was interrupted by Gu Qiang's angry roar.

"Shoot in!" Gu Qiang stood up suddenly, wild like a rabbit that had completed the journey of life.

Everyone was also very happy to watch, clapping their hands and applauding, happy for Gu Qiang's iron-blooded injection in the last five seconds.

Only Su Fan looked ahead in a daze, as if something had happened that he didn't want to recall.

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