Wang Liangcai still came early, in fact, he came earlier than before.

The main machine time was adjusted to morning, so that Wang Liangcai no longer had to worry about tidying up time and vacating space, etc., and could concentrate on completing the experiment. The most important thing is that he can do more than just prepare for experiments and preliminary experiments in the early morning, and there is no problem in conducting formal experiments.

In order to make the most of this part of the time, Wang Liangcai only watched one episode of Dream of Red Mansions after dinner and went to sleep, and in the face of the only entertainment, he could still be ruthless, because this project is too important.

Wang Liangcai is very fortunate that he was selected for this national project.

How important is the sheep cloning project?

For genetics scholars, this is basically the No. 1 project.

If cloning technology matures, all breeding and breeding will be a thing of the past, and the history of forced mating of cows, sheep and horses will be gone forever in tents, just as blacksmiths are to steam engines. They will still have jobs, but more often than not, they will only work on unimportant scraps, or to save money, or for the sake of art, whichever way, is meaningless.

However, no matter how important the cloning is, if Wang Liangcai clones another eighty pairs of bear-hearted leopard galls, he will not dare to start the project himself.

There is no need to pull anything else, an inverted phase contrast microscope cannot be bought by Wang Liangcai's laboratory.

Not to mention, such a project is bound to compete with foreign counterparts.

Even if you don't say what you're doing, foreign people who are engaged in genetic science are also approaching this goal step by step.

As long as you think about the world's leading genetic engineering laboratory, it is possible to get involved in the period, and Wang Liangcai feels that the pressure has doubled.

It is in Yang Rui's genetic engineering laboratory that Wang Liangcai's mood can be calmer, and he can do experiments with peace of mind without thinking about so many miscellaneous things.

For Wang Liangcai, this is the state he has longed for for a long time.

In comparison, watching one less episode of Dream of Red Mansions every day, going to bed a few hours early, and waking up a few hours earlier are nothing.

Whoever took the college entrance examination did not do it like this.

What teenagers can do with courage and perseverance, but people can't do it in middle age?

That's how you live and go back.

Led by Wang Liangcai, many researchers in the laboratory came earlier than usual. The most obvious is Su Fan.

Now that Su Fan is an old man in the ion channel laboratory.

He was one of the first to dig into the ion channel laboratory when Yang Rui was digging calves, and later he also opened his own research group, specializing in cloning-related research.

It should be said that his research group still has a very good output, and he gave considerable help when he did the embryo transfer of cattle in the genetic engineering laboratory of Haidian District.

Later, Su Fan joined the G protein-coupled receptor project and took on a lot of work.

No matter what period of evaluation criteria it is, Su Fan can be regarded as a small accomplished scientific researcher.

However, the most fearful thing in everything is comparison.

Su Xiankai, who joined the ion channel laboratory at the same time as Su Fan, started as a dog and independently started the G protein-coupled receptor project, and Su Fan joined in later, not even as a deputy for Su Xiankai, but after Fan Zhenlong and Gu Qiang, it was the existence of the third-in-command and the fourth-in-command.

Other people may be able to endure it, but Su Fan can't bear it at all.

Su Fan, who graduated from Beiyan Agricultural College, has always been sensitive to differential treatment. may have suffered a lot of discrimination, so since a long time ago, Su Fan has been very concerned about other people's eyes and arrangements.

Academic discrimination in academia is the most unruly discrimination, and in order to break its confinement, it is necessary to separate academic qualifications and abilities.

Emphasizing one's own abilities to prove that one's academic qualifications are the result of playfulness when young and ignorant is a common means of academic discrimination.

The same goes for Su Fan.

It wasn't until the previous unit tore the fig leaf that ability determines the job title that Su Fan jumped to the ion channel laboratory.

In the ion channel laboratory, Su Fan had a comfortable life for a while, but psychologically, his pressure did not lessen.

The prefix of the ion channel laboratory is Peking University, so the most Su Fan has seen in the laboratory are researchers from Peking University. Yang Rui, Xu Zhengping and others did not say, the later Jiao Yangping, Wang Sisheng and others were from Tsinghua University, and then Professor Man Hai, Professor Feng Junming and Professor Tao Xuelin were also from Peking University, which touched Su Fan a lot.

The work some time ago was too intense, so Su Fan didn't have time to think about it all carefully.

After being dispatched to the genetic engineering laboratory, Su Fan's heart of comparison could not be suppressed.

Especially compared with Gu Qiang, Su Fan disagrees with the latter even more.

Gu Qiang also didn't graduate from a prestigious school, so why should he bring a research group?

Although Gu Qiang has completed a number of experiments in a row, Su Fan still wants to compete with it.

Of course, cultural people can't just call the board, that's too ugly. Su Fan decided to learn from Wang Liangcai, one step ahead of Gu Qiang, and made the subject of cell enucleation.

said that it was studying, but in fact, it was to help Wang Liangcai.

If not, Su Fan would not be confident that he would be able to complete the work of cell enucleation.

Unlike Wang Liangcai's long-term training, Su Fan himself has not received the practice of "egg peeling", Beiyan Agricultural College does not teach this, and after joining the work, he can't find anyone to learn. Therefore, conducting experiments independently is completely self-inflicted.

Su Fan not only had the mentality of stealing a teacher, but also had the attitude of helping, and began to give Wang Liangcai a hand. He wanted to study for a while before considering whether to restart the stove or open up a new path.

However, the technology mastered by Wang Liangcai obviously greatly exceeded Su Fan's expectations.

Everyone is peeling eggs, and Gu Qiang's ability to peel eggs can basically only be said to be enough, nothing more than peeling them down.

Wang Liangcai's standard technique has been practiced for many years, and there are too many places to learn.

After running with Wang Liangcai for a few mornings, Su Fan got up earlier and earlier.

The interaction between the two unsurprisingly fell in Gu Qiang's eyes.

To say that there is no rush at all is a bit false. The same members of the same research group are also competitive, at the very least, when the paper is published in the future, everyone has to compete for a 3579 out.

But there's no use in being anxious. Tong Dizhou's apprentices and grandchildren, they are all egg-stripping stars, Gu Qiang, who was born in Shanda, although he has strong experimental ability, but in terms of egg peeling, he really has no advantage.

As the days passed, seeing that Wang Liangcai and Su Fan were cooperating more and more tacitly, and the two of them also transitioned from the visible nuclear experiment to the nuclear experiment, Gu Qiang could no longer sit still.

"Director Yang, I want to change an experimental idea. Gu Qiang's research group can only carry out experimental operations within the specified range, and Yang Rui must agree to change his ideas.

Yang Rui is well aware of the recent atmosphere in the laboratory, but he is happy to do so, doing scientific research, three hours a day, eight hours a day, and eighteen hours a day, whether there is competition or not is very different.

The healthy competition in the laboratory couldn't be better, Yang Rui asked unexpectedly, "How do you want to change?"

"I want to do a one-stop experiment, I don't want to do it step by step. Gu Qiang also used a killer move.

The steps of cloning are actually quite simple, the core part is to enucleate the egg cells of a sheep, stuff them into a new egg cell, and then stuff them into another sheep, and the lamb born is a cloned sheep.

Although there are many steps before and after, such as superovulation, such as taking care of pregnant sheep, etc., any step is indispensable. However, there aren't many core steps.

Doing it step by step is not only practice, but also finding ways to achieve a higher success rate.

For example, the last step is that you have to let the ewes give birth to a healthy lamb, and if you can't find a way to improve the success rate, the previous steps are not all wasted.

When the Scots were cloning sheep, they failed three times in the last step, each time it was a few months old, and this hard work can be experienced when you think about it.

However, the formal method is actually not particularly suitable for Gu Qiang.

He is not an experimental biologist with a good theoretical level, but more like a technician in a foreign laboratory. Although the latter also plays theory, they both play specific operations, and the difference between the two is like the difference between an automotive engineer and a car mechanic.

A good car mechanic is sometimes better than an automotive engineer to make a modified car or something, but you still have to be an automotive engineer to develop new technologies step by step and improve the technical indicators of every part of the car.

Gu Qiang also knows his shortcomings, and after doing it for a while, it is difficult for him to improve the accuracy rate in a separate link, which is not a problem of experimental techniques, but a problem of small ideas for small experiments.

Therefore, Gu Qiang simply proposed a one-stop start.

Yang Rui couldn't help but be silent, he also knew Gu Qiang's fault, but the reason why doing it step by step is a formal method is because when doing it step by step, the overall success rate is higher, compared with a dragon, it costs less resources, gets more results, has a higher reproducibility rate, and more importantly, saves time.

A sheep is pregnant for a few months, you do it all the time, one failure is equivalent to a year, and there are so many failures.

However, considering Gu Qiang's strong luck, Yang Rui was a little hesitant.

If it is really according to the formal academic judgment standards, Gu Qiang's level is not as good as Wang Liangcai's.

Although in terms of experimental ability, Gu Qiang is also first-class in China, but Wang Liangcai is the top in the Chinese Academy of Sciences, in terms of domestic scope, he is better than his technology, he is over the standard, younger than him, and his theoretical level and experience are far behind......

It can only be said that Gu Qiang is lifting a stone to shoot himself in the foot, and he named Wang Liangcai as soon as he opened his mouth, but he didn't expect that other people's skills would fully cover him.

Except for "strong luck".

Thinking of this word again, Yang Rui couldn't help but raise his eyes to look at Gu Qiang, who grinned an embarrassed but polite smile.

"If you change the dragon, the budget will change. Are you prepared?" Seeing that Gu Qiang has made breakthroughs in several scientific researches, Yang Rui is still relieved.

Gu Qiang hurriedly said: "I have written the report." ”

"What's going on?" Yang Rui glared at him, there was something that he didn't deliver, so he said what was going on.

Gu Qiang hurriedly went back to get it, turned around and handed it to Yang Rui, and said with a dry smile: "I didn't expect you to agree." ”

"I mean, shouldn't I agree?"

"No, it's not ......," Gu Qiang smirked twice. He originally thought that Yang Rui would have to bargain, such as splitting a dragon into two dragons or something, so as to reduce costs and risks, but he didn't expect that Yang Rui didn't ask for a bargain.

"The expected funding is 6 million yuan, plus 300,000 US dollars?" Yang Rui smiled when he saw the final figure of the report.

This is also his laboratory, out of Yang Rui's laboratory, there are only a handful of laboratories in the country that can give this funding.

Of course, Yang Rui's laboratory itself is one of the few high-end laboratories in China, but this amount of funding is still very exaggerated.

Previously, Yang Rui did a big project, and the total funding fluctuated up and down at this number.

It can be said that in China, 10 million yuan of funding is the ceiling, to reach this figure, not to say how the results, how prestige can be obtained, you get big results, at the same time have great prestige, trust, good resume, but also to find the right project, to be eligible to compete for such a funding value.

Gu Qiang is far away from such qualifications, so it is far to the edge of the sky.

With a wave of his hand, Yang Rui removed the 0 behind 6 million and 300,000, and said: "600,000 plus 30,000 US dollars, almost 1 million funds, it must be enough to buy consumables." ”

"I still want to buy a few instruments......"

"Make something and talk about it. "It is impossible for Yang Rui to approve more funds for Gu Qiang alone.

If anything, 1 million is overloaded.

He only leads a research group, not a project team, and all the honor belongs to the Lord, so the big expenses such as purchasing instruments cannot be decided by him.

However, if Gu Qiang can compete with Wang Liangcai this time and make something with one dragon, it will be different.

Opening another project team in the laboratory is actually a very good thing for Yang Rui.

A scholar can't be an emperor, at most he can be a Beiyang warlord, and there is a faction in the valve, which is not only normal, but also healthy. On the contrary, those who want to engage in dictatorship end up in disrepute.

Gu Qiang didn't think about Yang Ruiyuan, and he was not satisfied with the funding of 600,000 + 30,000 US dollars, gritted his teeth, and said: "Look at it, the progress on my side must come from behind." ”

"That's backward?"

"No...... It's not lagging behind. Gu Qiang hurriedly pleaded: "My current success rate of nucleation is almost 50%, and the success rate of nucleation is not low, I just think it's too boring to do it step by step......

His voice is getting smaller and smaller, because his success rate is lower than Wang Liangcai, and the number of things can't be argued.

"Director Yang, then I'll go back first?" Gu Qiang saw that Yang Rui was still reading his rough and rotten report, and he was inexplicably a little weak.

"Wait. Yang Rui stopped him slowly and said, "Your nucleus is the nucleus of the embryonic cell that is ready to be used in the blastocyst stage?"

"Yes, do you think the blastocyst stage is not suitable?" Gu Qiang humbly asked.

There are multiple stages in the development of an embryo, and it is common to use blastocyst stage cells.

Yang Rui shook his head and said, "I have no opinion on the blastocyst stage, I have an opinion on the embryo." ”

"Would you like a different kind of sheep?"

Yang Rui shook his head again and said, "My idea, can I try non-reproductive cells, for example, somatic cells such as breast cells?"

Gu Qiang couldn't stand still: "How can the nucleus of somatic cells be used to inject nuclei." ”

"How do you know if you don't try it. Yang Rui said: "Didn't I mention it at the project briefing?"

"I thought it was a vision. Gu Qiang's heart was palpitating, and he was more in denial.

Yang Rui said: "Since you have decided to do a one-dragon experiment, then don't wait." ”

"This ......" Gu Qiang regretted in his heart.

Before the birth of cloned sheep, including Chinese biology textbooks, the specificity and totipotency of biological cells were listed as important theories.

To put it simply, according to the current understanding, a cell is totipotent during the embryonic period, that is, it can do anything in the future.

The cells that are differentiated into skin tissue, blood, and heart are specific, and theoretically it is believed that they cannot grow in reverse, unlike embryos, which can differentiate whatever they want.

Taking fertility as an example, it means that there are only a few of them who have the ability to have children, and the others cannot be used for childbearing.

Cloning Dolly the sheep is exactly what really breaks the confinement.

Maybe for the average person, what is the nucleus of a cell that is not? But for biologists, it's a seismic change.

It is not an exaggeration to say that the biological edifice has collapsed.

Although the biological edifice collapses every year, this time it is indeed more powerful.

Therefore, although Yang Rui began to explain his ideas from the beginning of the project, including Gu Qiang and others, he still regarded it as science fantasy.

And fantasy shines into reality, sometimes it is beautiful, sometimes it is more difficult than a nightmare.

(End of chapter)