In the germ laboratory, there are already a few people busy.

Rows of wooden shelves are filled with glassware, and microscopes are resting on long tables. The pool, the worktop, all kinds of knives and oddly shaped glassware look very strange.

Although it was April, Ma Yilong couldn't help but shudder.

Pang Xian stepped forward and reported to Li Shizhen and Li Yuanming, who were studying under the microscope, before the two looked up at Zhao Hao.

"You've come just in time, look." Li Shizhen greeted Zhao Hao excitedly.

"What did you find?" Zhao Hao walked over with interest, stood between Li Wan and the two, and pointed one eye at the eyepiece of the microscope.

I saw a thin layer of solid medium in the glass petri dish...... This is made by boiling the seaweed into a transparent agar, and then adding a little nutritious gravy, which can not only cultivate bacterial growth, but also facilitate observation. This method is still being used four hundred years later. In this time and space, it is naturally counted as one of Zhao Gongzi's many scientific inventions that are not worth mentioning.

Of course, the medium is not the focus, the focus is on the small circular dots on the medium.

"What kind of coccus is this?" Zhao Hao asked.

Li Yuanming replied from the side: "We have found this coccus from the purulent wounds of different patients. According to the method taught by Childe, after staining with iodine, it is golden yellow when observed under the oil microscope, which should be the Staphylococcus aureus that Childe said. ”

"Oh." Zhao Hao nodded. Later generations with a little common sense know that this is the most common type of germ, which can cause various types of infections and is zoonotic. It is the biggest threat after a person is injured.

But for the healers of the Ming Dynasty, this is a great discovery.

But obviously, it's not this that excites Er Li, because this plate is ...... Oh no, they didn't stain this cocci, so the purpose was to avoid contaminating the test results!

"Childe, look below." Seeing that his eyes were leaking, Er Li jumped to his feet in a hurry. "So obviously you can't see?!"

Under the guidance of the two, Zhao Hao discovered that in the culture medium full of cocci breeding communities, there was also a miserable white circular area, which he ignored because it was too obvious.

"Is this a cocci melting?" Zhao Hao finally understood, and became interested: "How did you do it?" ”

There are too many things that can kill yellow coccus, such as alcohol and iodine, so it is not uncommon to be able to do it, the key is how to do it.

"Use this!" Li Shizhen picked up a speckled orange.

"Damn, you've isolated penicillin!" Zhao Hao became excited. Oh my God, it's finally time for this day......

Why is Zhao Gongzi always so cautious? He never drinks raw water on weekdays, and fruits and vegetables have to be scalded in boiling water before eating, refusing intense activities, and not daring to do any dangerous actions. It's really because I'm afraid of bacterial infection.

In this era of lack of medical treatment, if you can't make a small hole, or eat something bad, it will cause a bacterial infection, and then die.

The last time his brain was broken at sea, Zhao Gongzi was so worried that he was about to cry......

So the main purpose of his establishment of Jiangnan Hospital ...... One? is to do everything possible to improve the level of medicine in the Ming Dynasty and increase his chances of survival when the injury strikes.

Penicillin, as a life-saving artifact, is naturally the top priority of research and development.

He watched the Japanese drama "Benevolent Doctor" in his last life, and he still remembers the plot of the southern doctor who traveled through ancient times to produce penicillin. Did he know anything at the time? said that there are three medical doctors who do medical guidance for screenwriters, that method should be reliable...... bar.

At the beginning of the establishment of Jiangnan Hospital? Zhao Hao told the three miracle doctors about the method of extracting penicillin from the southern doctors. In general, oranges are sterilized before being made into a culture medium. And then inoculate penicillium from moldy oranges? Constant cultivation and reproduction.

After propagation to a certain number, filter the culture with a funnel? Adding canola oil to a filtered liquid? Stir well in a bucket? Stratification of liquids – oil-soluble substances in the upper layer? Insoluble substances in the middle, water-soluble substances in the lower layer.

Penicillin is a water-soluble substance. So install a faucet at the bottom of the bucket to get penicillin dissolved in water.

Next, pour it into activated charcoal sterilized with boiling water and stir. Filtered with distilled water? Wash off clutter. It is then filtered with acidic water made from white vinegar, because penicillin is weakly acidic, so it does not dissolve in acidic water, so that alkaline impurities are removed.

Finally, it is filtered with baking soda water, and the result is a high-purity penicillin solution.

It sounds very simple, but Zhao Gongzi also thinks that it is not very difficult, and he said that it can't be a stupid method of 'separation-selection-purification-selection'. Vigorously produce miracles, oh no, there is great strength in numbers, and penicillin must be extracted.

However, Jiangnan Hospital has been established for two years, and the three divine doctors and their disciples have worked day and night, and only now have they made a breakthrough......

But a good meal is not afraid of being late.

"Hahaha!" In the laboratory, Zhao Gongzi's smug laughter sounded: "Ben Gongzi finally saw penicillin alive!" ”

~~

Of course, Ma Yilong didn't know why Zhao Gongzi was so excited, he felt like he was an idiot as soon as he entered this room.

Li Shizhen brushed open the curtains and shouted to Zhao Gongzi, who was dancing excitedly: "Calm down, don't break the equipment!" ”

"Uh......" Zhao Hao held on to a flask and said with a dry smile: "I'm sorry, I'm too excited." ”

"Childe, don't get excited too early." Li Yuanming smiled bitterly: "The petri dish you saw is the only one that has succeeded in this experiment. ”

"What about the rest?" Zhao Hao hurriedly asked.

"It's all here." Li Yuanming opened a cupboard, and hundreds of petri dishes were neatly stacked inside. "Previously, we put the penicillin solution we obtained into these petri dishes, and only one successful sample was obtained."

"This is the labor success from the beginning of the year to the present." Li Shizhen said gloomily: "It is indeed proved that penicillin can be extracted from Penicillium to kill bacteria, but after three months of busy work, the penicillin obtained cannot even kill the cocci in a petri dish. ”

"Spit is better than it." Li Yuanming's violent temper, if he wasn't wearing a mask, he would have to spit into the petri dish.

But he was able to say such things, and he had apparently tested the bactericidal effect of saliva......

"Don't do that, that's a good thing." Zhao Hao suppressed the disappointment in his heart, and secretly said that he had to continue to survive...... But as a cheerleader, of course, he had to smile and cheer up the doctors: "Isn't everything done from scratch, from coarse to fine?" It's only been less than two years, and it's already grown from scratch, which is gratifying! ”

"That's right." As the dean of the medical school, Li Shizhen certainly has to maintain an optimistic attitude. "We didn't do it for a year last year. It was only in the winter that progress began, and it was later discovered that the penicillin was not heat-tolerant and did not multiply at all in the summer. Jiangnan has an exceptionally long summer ......"

"Uh......" Zhao Gongzi nodded secretly when he heard this, as if this was the case. But as if he forgot to say it, in order not to be burned by Er Li's anger, he also pretended to be abrupt and said: "No wonder I couldn't copy it at the beginning, it turned out to be this reason." ”

"Childe can find penicillin, it's already amazing, okay?" Li Shizhen said with an expression of how you are not satisfied:

"Penicillium of unknown origin is needed to fall into your staphylococcal medium. It was still a cool early spring, allowing the penicillium to grow and mature and produce penicillin. Then the temperature rises, which is conducive to the rapid growth of staphylococcus, so that the phenomenon of bacteriolysis occurs......" Li Yuanming also looked at Zhao Hao with an incredible face and said: "And I haven't washed the petri dish for more than a month, if I am in medical school, I will be expelled eight times." ”

"It's called no coincidence and no book." Zhao Gongzi snorted, anyway, Fleming discovered penicillin in this extremely coincidental state.

However, due to the technical conditions at the time, Fleming was never able to isolate penicillin alone. It wasn't until before World War II that a little penicillin was isolated by the Americans. It is said that at that time, penicillin could only be used by one person in the entire United States in a year......

Thinking about it this way, Jiangnan Hospital spent a lot of effort to come up with this bit of penicillin, which is very normal.

"What's next for you?" Zhao Gongzi diverted the topic and asked.

"Keep trying, there are still a lot of things that can be improved." Li Yuanming said: "For example, purify baking soda and see if it will be better." ”

"Well, that's the way." Zhao Hao thought for a moment and said: "But the key is to breed more penicillium, maybe citrus cultivation is not the best choice." And it's not just citrus that has Penicillium......"

"Uh......" Erli looked at Zhao Hao speechlessly: "Why didn't you say it earlier?" "They've always had a preconceived notion that only Penicillium citrus will do......

"I'm guessing too." Zhao Hao wiped his sweat and sneered: "Didn't you say it earlier, you can't be superstitious about authority, you have to have the courage to try." ”

"Okay." The two nodded, silently figuring out where to extract the mold.

"That's how you're coming to me?" Zhao Hao inevitably asked with some disappointment.

"It's not." Li Shizhen said lightly: "The allicin extraction has been successful. ”

"I'll rub, don't say it sooner! Follow me, if you want to promote first, suppress ......," Zhao Gongzi smiled.

Compared with the difficult penicillin, the extraction of allicin is much simpler. Garlic is mashed and left for one hour, and then soaked and extracted with high-concentration alcohol, a low-concentration alcohol solution of allicin can be obtained. Or it can also be obtained by steam distillation, allicin solution has a killing effect on a variety of cocci bacilli, and also has an inhibitory effect on fungi, which is a low-profile multi-purpose version of the life-saving artifact......

"Uh, I'm sorry, excuse me......" Seeing that the three of them were about to discuss what to give allicin with great interest, Ma Yilong, who had been waiting for a long time, finally couldn't help but ask: "Can this penicillin, and allicin, cure silkworm disease?" ”

"Uh......" The three people who made it hot only remembered that there was another one here.

"Sorry, I don't know." Li Shizhen shook his head honestly and said, "It's just that I haven't caught him for a long time, so report the progress of the work to him." ”