When Li Hongyu was afraid that people would go to the Spanish military camp to find Captain Rohr, Edward led Yang Li'er to the market to buy the items needed to make penicillin.

Since Bangkok was a commercial port where merchants from the East and the West gathered, it was easy for Edward to buy glassware for penicillin.

The first step in extracting penicillin is naturally to prepare penicillium, and Edward and others naturally cannot have ready-made green plums on hand, so they have to make them themselves.

According to Li Hongyu's confession, Yang Li'er asked people to buy a large number of steamed buns, steamed buns, fruits and noodles in the street market, and then grind the rice into juice and mix it with it, so that Penicillium can be produced.

Next, the juice boiled from taro is used as a culture medium, and the prepared Penicillium is put into it for seven days.

After seven days, the culture medium is filtered with a filter cotton, and the filtered culture medium is added to rapeseed oil to dissolve the lipo-containing substance, and since penicillin is water-soluble, the water under the oil dissolves penicillin.

At this point, the next step is to extract penicillin from the water, add boiling sterilized activated charcoal to the water containing penicillin, and stir it in a container.

After stirring, put the activated carbon into a separator tube with a large opening at the top and a small opening at the bottom, wash it with distilled water, and then inject acidic water made of vinegar to remove impurities, because penicillin is a weak acid and therefore will not be soluble in weak acid.

At this time, the last step of penicillin extraction is left, and the alkaline water boiled into juice through seaweed is separated from the toner using a double funnel, and the lower end of the funnel flows out of the relatively pure penicillin.

Although penicillin was extracted, since penicillin was made, it was necessary to test its efficacy to see if it was effective.

The method is simple: divide the resulting penicillin into equal portions, label them, and then add a small amount of penicillin solution to the bacteria that can be inhibited by penicillin in advance, and this process is carried out on a piece of paper with the same number as the penicillin used, so that it can be observed in the future.

If a circle appears around the piece of paper, it means that the bacteria are expelled, indicating that the corresponding penicillin is effective, and no change indicates that it is not effective.

After the efficacy is determined, it is necessary to carry out the final step of the whole penicillin extraction process, and find a way to refine the high-purity penicillin, the higher the purity, the greater the efficacy on bacteria.

The method is to coat the prepared penicillin on the paper and hang it vertically, dip its end into a solution made of two parts acid and eight parts alkali, and use the different RF values of the dissolved original drug substance to layer on the paper, and then refine the high-purity penicillin.

If the whole process is to be completed, it will take about ten days, Edward, Yang Li'er, and Youlan dare not have the slightest slackness, in order to save Li Hongyu, they have to race against time, race against death, and strive to make penicillin before Li Hongyu's condition deteriorates.

Captain Rohr heard that Gonzalez was looking for him, and with Fidel's permission, he went with the sailors to meet Gonzalez.

Fidel was already preoccupied with the shooting, and not long ago, the King of Siam, who had arrived in Bangkok, learned that the men of the Ming fleet had surrounded the Spanish barracks, and immediately ordered his soldiers to go to the aid of the sailors and fight against the enemy.

This exacerbated the already poor situation of the Spaniards, and the rest of Europe in Bangkok watched from the other side, happy to see the Spaniards deflated.

Speaking of which, the Spaniards had a very bad relationship with many European countries because of their strong maritime power, and fought with many countries in order to compete for interests, among which the British naval battle was the most famous, and it is still in a state of war with the Netherlands.

Barely calculated, the only reliable ally of the Spaniards is Portugal, which is adjacent to the country, but Portugal is now very close to the Ming Dynasty, and it will definitely not lose the huge market of the Ming Dynasty for Spain.

As a result, the Spanish soldiers and merchants in Bangkok were practically isolated, and it only took a single assault battalion of the besieged Daming Water Division to fall.

Fidel very much wanted to see the senior commander of the Ming fleet to negotiate, but unfortunately Wang Hong and Bai Cheng ignored him at all, so he could only order the soldiers to be on guard twenty-four hours a day, and the only way to fight with the Ming was a last resort.

"Gonzalez, they didn't torture you?" When Rohr saw that Gonzalez was safe and sound, a look of surprise suddenly appeared on his face, and he thought that Gonzalez would be tortured by the Ming people to extract a confession.

"Mr. Captain, do you know of any soldiers in those European countries in Bangkok who are both accurate in marksmanship and have been in Bangkok for many years and are familiar with the terrain of Bangkok?" Gonzalez nodded, then asked in a deep voice.

"Good marksmanship and familiar with the terrain of Bangkok?"

Rohr glanced at Gonzalez with some surprise when he heard this, and then he didn't understand why he was asking about it, and then said, "In Bangkok, there are two people with the best marksmanship, one is French, the other is British, if you are familiar with Bangkok, then it is the British. ”

"Who is he?" Gonzalez's eyes lit up suddenly, and he hurriedly asked.

"His name is Lyman, and he is a captain of the Englishman at the camp in Bangkok, and I have tried his marksmanship against him, and he is no match for him." Hearing this, Rohr asked with some curiosity, "Why are you interested in him?" ”

"I've seen the general story of Daming's injury, the person who duel with me yesterday, he believes that I didn't send someone to shoot him, so he wants to start with the gunman to find the murderer behind the scenes."

Gonzalez groaned for a moment and whispered to Rohr, "That Lyman is very suspected of being a gunman, otherwise it would be difficult to hit Da Ming's general affairs in the current environment." ”

"That kid is a general of Da Ming?"

Rohr was also at the scene of the duel between Gonzalez and Li Hongyu, and was one of those Spanish officers, and was very surprised when he heard this, and then nodded thoughtfully, "Indeed, it is difficult to hit the target at a bonfire party with many people. ”

"These abominable Brits!" As he spoke, Rohr couldn't help but slam his fist on the table, and said hatefully, he seemed very disgusted with the British, and his father died in the naval battle with the British.

"As long as it can be proven that Lyman is the gunman, then we can clear the suspicion." Compared with the indignant Rohr, Gonzalez breathed a sigh of relief, and as for how to settle accounts with the British, then it was not a matter for him to consider.

"However, Lyman had already left Bangkok three days ago." Hearing this, Rohr couldn't help but think of something, and couldn't help but say to Gonzalez with some depression.

"What?" Gonzalez was stunned when he heard this, which was beyond his expectations, and he never expected that Rohr had already left.

"What about the Frenchman? Is it possible that he was the gunman? Gonzalez then nervously asked, now that Rohr was gone, it was clear that there was no longer any suspicion, and that his hopes were pinned on another Frenchman with good marksmanship.

"That Frenchman has only been in Bangkok for more than three months, and he is not familiar with the local terrain, and if it were him, I am afraid that he would have been caught by the searchers." Hearing this, Rohr shook his head and said helplessly, the French did not meet the standards of gunners.

"Could he have scouted the terrain in advance?" Gonzalez asked tentatively looking at Rohr with some reluctance.

"The location of the bonfire was improvised, and he couldn't scout so many places for three months." Although Rohr was reluctant to hit Gonzalez, he was telling the truth, the French really did not have what it took to be a gunman, and there was no way to escape after firing a gun.

"How so!" Gonzalez's eyes suddenly showed a look of disappointment when he heard this, and the hope that had risen in his heart was ruthlessly shattered, and if he could not find the gunman, Cole would be charged with the gunman, and he and Spain would be inseparable.

"Maybe it was the other gunmen, but we can also list those who are a little less good at marksmanship as suspects." Seeing this, Rohr opened his mouth to comfort him, so that there were several suspects.

"Mr. Captain, I hope you can keep it a secret until the matter is clear, so as not to alarm the mastermind behind the scenes." Gonzalez came back to his senses, nodded, and whispered to Rohr.

Hearing this, Luo Er suddenly showed a wry smile, now the Spanish camp is surrounded by the troops of Da Ming and Siam, even if he leaks it, it can only be known by the people in the camp, and it can't be spread to the outside at all.

After seeing Rohr, Gonzalez immediately went to find Li Hongyu, although Sakurako Yamashita was reluctant, but still woke up the sleeping Li Hongyu, Li Hongyu explained that if Gonzalez came to see him, it must be an important matter, and it could not be delayed.

"That Lyman left Siam three days ago?"

After learning the news provided by Luoer, Li Hongyu, who was leaning on the hospital bed, suddenly frowned, feeling that the matter was a bit too coincidental.

"Master Tongshi, this is the list of suspects provided by Rohr's superiors, you can ask someone to check it." Gonzalez handed Li Hongyu a piece of paper listing the best marksmanship among the soldiers of European countries in Bangkok.

"The information you provided is very useful, and I will have people look it up." Li Hongyu glanced at the list, and then said to Gonzalez with a smile.

Although he can also find out this information, it will take a lot of time, and it will also lead to the vigilance of the murderer behind the scenes, so it is better to find out the situation from the Spaniards.

"Sakurako, you send someone to tell Wang Hong, let him go to the King of Siam, let the King of Siam block the port of Bangkok, prohibit all ships from going to sea before the shooting case is concluded, and block the roads in and out of Bangkok."

After Gonzalez left, Li Hongyu thought for a while and said to Sakurako Yamashita who was standing next to the bed.

Although the gunman took advantage of the chaos to escape, because the Daming Sailor and the local government in Bangkok have been vigorously searching, he must still be in Bangkok, so Li Hongyu wants to trap him.

In fact, Li Hongyu already has a choice for who the gunman is, that is, Lehmann, who left Bangkok three days ago, and Lehmann has a very good alibi as soon as he leaves.

But then again, if Lyman, who is supposed to have left, is found in Bangkok, then the identity of Lyman's real murderer will be self-evident.

When Wang Hong told the Queen of Siam what Li Hongyu meant, the King of Siam did not hesitate at all, and agreed in one bite, and ordered the Siamese soldiers to block the water 6 two roads in and out of Bangkok.

The soldiers of the Naval Division did not sit idle, assisting the Siamese soldiers in the blockade, conducting a military drill, and at the same time withstanding the pressure from foreign merchants, especially Western merchants.

As soon as the notice of the King of Siam blocking the 6th passage of water was posted, it immediately caused a huge sensation in Bangkok, and the notice stipulated that no boat except fishing boats was allowed to go to sea, let alone leave.

This meant that the merchants in Bangkok would have to postpone the date of their departure, and the warships of various countries parked in the harbor could leave the port, but they would not be able to get in touch with the people on the docks, because the soldiers of the naval division and the soldiers of the Siamese State blocked the port and forbade anyone to enter or leave, thus avoiding the gunners from leaving on the warships.

Although the Western countries in Bangkok were quite dissatisfied with the blockade of the port by the Ming Dynasty and Siam, everyone had to endure it for the sake of an assassination of a Ming official, and no one was willing to challenge the Ming Dynasty at this time, so they chose to wait and see what happened.

As for those businessmen who want to leave the port, they dare not challenge the prestige of the Ming Dynasty, so they can only complain in private, so as not to anger the Ming Dynasty and end up with the scene of losing both people and goods.

This is an important reason why Li Hongyu came with the fleet, because it was the first overseas trade, if there were some unexpected events, it would be difficult for Wang Hong and Bai Cheng to make a choice, and some things related to the relationship with overseas countries, which were already out of their authority.

It's just that Li Hongyu didn't expect that the problems encountered by Daming in his first overseas trade would be related to him, and he still had to fall into a life-threatening situation.

When Wang Hong and Bai Cheng secretly searched for Lyman, Li Hongyu's condition became more and more serious, and Edward's own concoction did not have much effect on Li Hongyu's condition.

Seven days later, Li Hongyu was in a high fever and had to be physically cooled down, and his mind became a little blurred, but unfortunately penicillin had not been successfully extracted at this time.

In fact, Edward knew very well that if it weren't for Li Hongyu's good physique, he would probably be difficult to survive until now, and he was helpless about it, so he could only do his best to help Li Hongyu cool down, which was the only thing he could do now.

Edward didn't know if Li Hongyu could survive until penicillin was extracted, let alone whether penicillin would work, so he could only resign himself to fate, and secretly prayed for Li Hongyu.

After this period of time together, Edward respected this young Ming official, although he was young, he had a gentlemanly demeanor, and he was full of generosity and wisdom when dealing with people, and he liked to deal with such people very much.

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