One did come back, not only did he come back, but he also brought a few women who were about thirty years old from Xiangyang Mansion and settled them in the small courtyard where the horse widow used to live temporarily. Pen & Fun & Pavilion www.biquge.info

After the incident between the horse widow and the uncle Liu Xiaozhong broke out, the two were imprisoned in the county government together, and later Liu Xiaozhong was released by mistake because of Liu Yuanshan, and the horse widow became the concubine of a small official. The horse widow's dwelling was left unused.

This place originally belonged to the Wang family, but Wang Baozhuang of the Wang family was Liu Yuanshan's first-class agent, and it was naturally not difficult to arrange for a few women to live in the courtyard for a few days.

Penicillin was successfully cultivated, but there were more than 60 specimens in hand, which ones could not be used and which ones were effective, and this thing could only be determined after passing the test.

You can't use Xue Huan as an experiment, so you can only use these dusty women.

Speaking of which, it was not that penicillin was injected into their bodies for direct observation, and Liu Yuanshan was not such a cruel biochemical madman, his method came from the divine book.

These women were all carefully selected after exploration, and they all had willow disease.

In the case of gonorrhea, some gonorrhea can be extracted from the necrotic wound, which belongs to the Gramerian bacterium and is most sensitive to penicillin, so that some useful penicillins can be screened.

Of course, Liu Yuanshan is not going to use Graft bacteria, but Staphylococcus that exists in symbiosis with Graft bacteria at the wound!

After all the penicillin had been extracted, Liu Yuanshan asked a person in charge of extracting the bacterial tissue fluid from the women who had lost their feet for large-scale culture.

Among them, the specific extraction method is bloody and pornographic, and it is inconvenient to say more.

Anyway, after two hours of hard work, the tissue fluid containing staphylococcus and Gramser bacteria was in his hands, and Liu Yuanshan was not too dirty, so he brought a mask and simple gloves and personally divided all the tissue fluid into more than 60 parts.

Then, start culturing the bacteria again with another set of Petri dishes.

However, when culturing bacteria, a drop of a solution suspected of containing penicillin will be dropped in the middle of each petri dish, and the cultured bacteria will be placed in a suitable environment according to the number, and the screening work will begin.

Without glass, you can't make a microscope.

Without a microscope, it is impossible to visually observe the bactericidal effect of penicillin.

But it doesn't matter, you can look at it indirectly.

Staphylococci are yellowish in color, and after a week or so of incubation in a petri dish, the entire surface of the medium will be covered with these bacteria, causing the entire surface of the medium to become somewhat yellowish in color.

Although you can't see the bacteria, you can see the yellow layer on it and you know that it's a staphylococcal colony.

Penicillin is the excrement of the fungus Penicillium, and the reason why it can kill bacteria is because it can hydrolyze the outermost cell wall of bacteria, causing bacteria to be unable to survive, so as to achieve the purpose of bacteriostatic purposes.

Animal cells only have a cell membrane and no cell wall, so theoretically penicillin does not pose a hazard to the human body.

But that's just in theory!

After about five days of cultivation, staphylococci developed smoothly, and Liu Yuanshan estimated that he should be able to see some clues now.

On the morning of the nineteenth day of Xue Huan's retreat, Liu Yuanshan entered the gentle room and began to open the petri dish in turn!

No. 1 opened, and the surface of the petri dish was indeed yellow, which proved that the staphylococci here were reproducing quite well, but after he saw the yellow medium, he was slightly disappointed.

This proves that penicillin solution No. 1 is not qualified.

Either the penicillium doesn't produce penicillin at all, or the penicillin it does not have the effect he needs.

Then, reach out and pull open the second petri dish, exactly like the first one!

There is still no penicillin.

Third ......

Fourth ......

Fifth ......

The more Liu Yuanshan looked at it, the more sad he became, he looked at five in a row, and none of them produced penicillin, could it be that there was a problem with my extraction method? Or is there an uncontrollable mistake in the middle?

Alas, leave it to fate!

Liu Yuanshan shook his head and continued to watch.

Sixth ......

Seventh ......

There is still no reaction, and the surface of the medium is covered with yellowish staphylococcal flora!

The eighth one was opened, and the clouds on Liu Yuanshan's face were instantly swept away!

Finally see a reaction!

Although the eighth medium is covered with a faint yellow, but in the middle of the medium, there is a very small blank area, where there has been a drop of penicillin, that is, the penicillium of the eighth strain can produce antibiotics, but the blank space in the middle looks like there are still some yellow silk threads, although sparse, but not really pure white.

In other words, the number eight can produce penicillin, but the effect is not ideal.

Liu Yuanshan was slightly disappointed, but the light in his eyes did not dissipate! This shows that your own method is fine, and the rest can only depend on luck!

The Ninth ......

Tenth ......

Eleventh ......

……

Liu Yuanshan looked at them one by one, and when he turned to the thirty-third petri dish, he finally released the suppressed emotions in his heart, cheered, and then breathed a long sigh of relief.

The perfect antibiotic appears!

On medium 33, the edges of the entire medium are faint yellow, which proves that there are staphylococci on it, but the big place in the middle is indeed a perfect blank, and the exposed medium is still the color of five days ago, and there has been no change at all.

This means that the solution that was originally dropped into the middle of the medium has a highly effective antibiotic.

"Okay, ...... number thirty-three"

Liu Yuanshan was overjoyed.

With this antibiotic, Xue Huan's wound doesn't have to worry!

With a cheerful mood, Liu Yuanshan continued to look through the remaining petri dishes, and in addition to No. 33, he found two more perfect strains of Penicillium.

Of the more than 60 cultured strains, three strains were finally confirmed to be penicillin-producing. In the future, if you want to mass-produce this thing, you can use these three penicillium strains as the mother body, and you can build a huge antibiotic empire.

With the magical effect of this thing on the wound of the cold weapon era, it is impossible to make money without it!

Hey!

Liu Yuanshan smiled slightly, walked out of the cultivation room, and basked in the sun outside the door for a while, full of physical and mental comfort.

At this time, the subordinates outside suddenly came to report, saying that they found that the temperature out of the underground palace had begun to rise, and the surrounding frost and snow had melted!

Could it be that Xue Huan is going to be out of the customs? It's only been 19 days?

Liu Yuanshan secretly wondered in his heart, but he didn't dare to delay, and ran to the gate of the underground palace with his subordinates at a trot, and sure enough, he saw that the temperature around him had risen a lot.

The milky white mist directly above the dungeon also gradually dissipated.

Xue Huan exhausted her strength to maintain the frozen state for less than twenty days, and now she is out of the customs! (To be continued.) )