When he heard that he was going to find a kiln sister to come over, he was stunned. Pen, fun, and www.biquge.info

When people's lives are at stake, how can they still be in the mood to find Sister Yao?

"Childe, this ......"

Liu Yuanshan smiled slightly, and couldn't explain again!

In ancient times, the medical and health conditions were poor, and most of the low-level women engaged in the color and green industry were infected with flower willow disease, some of which were gonorrhea in modern medicine, and some were syphilis that came from the Americas in the middle of the Ming Dynasty.

To test the function and effectiveness of antibiotics, gonorrhea patients are the best bacteria to test antibiotics for. Uh, no, isn't it better to say, anyway, this Nima's funny divine book gives gonococci.

It is estimated that it is because it is not easy for ancient society to obtain a sick body, so it is given such a strange thing as gonorrhoea!

Liu Yuanshan didn't think much about it, took out some photos of the characteristics of the gonorrhea patient's body, drew them on paper and gave them to him, and said: "You go to Xiangyang Mansion to find Sister Yao one by one, and if you have this kind of wound on your body, find me five or six back, and place them in Liujia Village." ”

The photographs were transplanted directly from the Book of God.

After one left, Liu Yuanshan's work continued.

All 60 or so media numbers were placed in a suitable environment to start the culture, and the growth of fungi in the medium was observed and recorded daily.

In this way, after about a week, penicillin was produced in a petri dish that was invisible to the naked eye. Liu Yuanshan followed the instructions in the divine book and began to extract.

In later generations, when penicillin was extracted by local method, the culture liquid in the petri dish was generally cleaned out, and then poured into the test tube, and at the same time, a mild liquid grease was added, and the liquid in the test tube was divided into three levels.

The top is oil, oil will dissolve some fat-soluble substances, what are these substances Liu Yuanshan and don't care about it, anyway, in the culture medium of antibiotics, they are all collectively called impurities.

The bottom layer is an aqueous solution, and since antibiotics are water-soluble substances, they are bound to mix with certain impurities.

Between the grease above and the aqueous solution below, there is a layer of suspension that does not dissolve in anything, there is no doubt that for Liu Yuanshan, this kind of thing does not ask what it is, it is impurity.

Liu Yuanshan has not yet produced glass, let alone such a thing as a test tube, so he can only replace it with dozens of specially made small bottles prepared in advance.

This small bottle is actually modified with a steel pipe, and the solution can be poured directly through the opening at the top, and there is a small opening on the side that can be closed to facilitate the release of the aqueous solution.

So, according to the description method in the book, Liu Yuanshan gently poured out the culture liquid in the petri dish and filtered it.

The funnel used for filtration is very easy to do, just make one temporarily, but unfortunately there was no filter paper in ancient society, which was a more troublesome thing for Liu Yuanshan.

However, it doesn't matter much, there is no filter paper, you can replace it with something else, and that is cotton!

I found a piece of densely woven white pure natural cotton cloth, cut it into a suitable shape, folded it twice, and laid it in the funnel, and then poured the culture liquid in the petri dish into the funnel, and after filtering through several layers of cotton cloth, the liquid flowing out of the funnel went through the first step of rough purification.

The liquid that comes out below is naturally a penicillin solution (if penicillin is indeed present in the culture medium).

However, the penicillin solution obtained by this method is too coarse, and the impurities in it are likely to be too many to count, so it cannot be applied directly, and it has to be purified with additional efforts.

The second step is to put the solution into a special small bottle, then add a certain amount of rapeseed oil to the bottle, close the lid and shake it vigorously.

After shaking, place the small bottle on the table and let it rest, and when it is expected that the contents will be roughly divided into three layers, open the side opening under the special small bottle, and the first thing that comes out of it is water-soluble penicillin.

In this way, penicillin passed the second round of purification, and this round removed a lot of impurities.

In order to save time, Liu Yuanshan must make the best use of time when doing work, so he can't take turns extracting all sixty cultures one by one.

Every step you take, you have to go hand in hand!

For example, when he used rapeseed oil to coat out fat-soluble impurities, he used more than 60 special small bottles to purify them at the same time.

The difficulty and intensity of this work is a bit great, and he is not at ease when other people help, so he can only do everything by himself!

But in this way, the advantage is also obvious, which is to save time.

After another round of purification, more than 60 copies of the culture were turned into a more pure penicillin solution, but this thing still cannot be used directly.

Follow the instructions of the divine book and begin the third step of purification.

The third step is to use a porcelain vase with a bottom that can close the opening.

A lot of high-temperature sterilized toner was placed in the porcelain bottle, and the solution extracted in the second step was put into more than 60 toner porcelain bottles, and then stirred vigorously to mix the toner and penicillin solution evenly.

In this way, the penicillin is adsorbed into the toner.

More than 60 porcelain vases are placed on the shelves and lined up in neat rows, which looks quite spectacular.

In the fourth step, distilled water is poured into the toner paste containing penicillin, followed by white vinegar.

It stands to reason that the fourth step should be poured into a relatively pure weak acid, but the only weak acid that can be found in ancient times is white vinegar.

Vinegar is a weak acid, and penicillin is also a weak acid, so that the two are insoluble with each other, but some of the weakly alkaline impurities will be conquered and removed by acetic acid.

Open the opening under the porcelain bottle and let the acid water out, which naturally takes away some of the impurities.

All that's left in the vase is black toner.

It doesn't matter, the toner at this time is not just as simple as toner, but also the adsorbed antibiotics.

In this way, the purity of penicillin is further improved.

After this method of purification, the penicillin can meet the requirements of use.

Next, separation!

The fifth step is to mix the weakly alkaline soda and charcoal that have been prepared for a long time, and then open the small hole at the bottom of the porcelain bottle, and a high concentration of penicillin will flow out of it.

Oh no, it should be said that it is a standard solution that may contain penicillin!

At this point, more than 60 high-concentration penicillin solutions have been extracted, and Liu Yuanshan numbered the solution one by one according to the number of the previous petri dish strains and sealed it.

At this time, fourteen days had passed since Xue Huan's retreat and practice.

"Hopefully, a few of these sixty or so solutions can be used!" Liu Yuanshan prayed secretly, his eyes looked in the direction of Xiangyang Mansion, and muttered to himself, "One, you should also come back." (To be continued.) )