First of all, it is to unwrap the twist first, and unravel the messy kernel.

Soon, the DNA strand of the single cell was presented to Hogu without reservation.

The next step is 'interception', which cuts out the part of the gene strand that expresses the phage, which is quite a delicate job, especially for Huogu, who now doesn't even have hands and feet.

The output of restriction enzymes is carefully controlled to ensure that the rest of the gene chain is not harmed, while the restriction enzyme is controlled by command, using the flow of cytoplasm to limit the restriction enzyme to the fragment of the gene that needs to be removed.

This act is tantamount to writing with saliva, and it is also writing on the water.

"Oops!"

As Hogu's chagrin appeared, the restriction enzyme was cut all the way, and other gene fragments were also cut, and the core of this single cell shrank by nearly half of its size after a while, until the restriction enzyme was exhausted.

Subsequently, in the ligase, which is instinctively secreted by a single cell, these fragments of genes are joined to each other and combined again into a complete gene chain.

However, Hogu knew that it was no longer possible for this greatly changed gene chain to replicate the original single cell.

Hogu re-selected new cells within the single-cell cluster as the target for 'surgery'.

The failure just now didn't give Huo Gu much of a blow to his confidence, he never thought he was a genius, and even felt that he was a little dumber than the average person, but he read a little more books than others.

Surgery restarts.

This time, learning from the lessons of the previous one, Hogu was more careful with the use of those intracellular restriction enzymes, drastically reducing the amount of restriction enzymes used, and focusing most of his attention on the movement of the cytoplasm.

Let the cytoplasm flow, direct those restriction enzymes, cut out the specified gene fragments, and at the same time, after the cropping, just deplete.

Fail......

Fail......

Fail......

……

Fail......

Succeed!

"Very good."

As Hogu believed, it succeeded, and after losing nearly 100 cells, the phage's genetic fragment was cropped.

The next step is ligation, which is relatively simple, Huogu remotely controls the cell, using the flow of cytoplasm to limit the gene fragment expressing the phage to a certain area.

Then, with the help of ligase, the DNA that had been split into two segments after surgery was reunited into one to form a new gene strand.

"Beginning to Split"

Hogu commanded in this way, and the replicator cell, which had undergone successful surgery, began to gradually divide into new individuals.

Soon, a new instance of the single-celled cluster appeared, but this new instance was still in a situation that Hogu had not anticipated.

That's the 'cell wall'.

The replicator cell is wrapped in a shell, which is stronger than the cell membrane and has a certain elasticity, which can give the protective cell enough room to grow.

"This ......"

"Why?"

Huo Gu thought about it for a moment, not very convinced.

"Could it be because of the expression of the mutilated phage gene?"

The operation didn't completely cut the phage's genes, Hogu just removed the part of the gene that he had learned about expressing the virulent phage.

In any case, the mutilated gene expression is also mutilated and incomplete, and it is impossible to transcribe the phage.

Risking the recurrence of toxic phages for perfect excision?

At that time, Huo Gu thought about it, but he still forgot it, he was not a perfectionist, and there was no need to die like that.

I didn't expect this to happen......

A cell wall is a must, because the main function of the cell wall is to protect the cell.

To put it simply, Buyi Diao Min put on steel armor, and his defensive power is not the same as before.

Originally, Hogu planned to start the manufacture of cell walls after solving the phage matter, but now......

Hogu was in a tangle.

It's not that it doesn't like such surprises, but after all, this is an 'accidental' cell wall, and it's hard to say whether it can meet the requirements it wants.

"It's decided, let's wait and see for a while."

Hogu made up his mind, turned his attention to the other single cells, and began the operation.

With the first successful case, there will naturally be a second and a third, and after a while, Hogu has become perfect with practice, and the success rate of cutting gene fragments has stabilized at more than 80%.

During this period, Hogu thought of a way to break the bond of the protein structure, that is, by dividing, between the two single cells that established the connection, to create a new replicator, and squeeze out the old single cell.

This is somewhat similar to the principle of the ship of Theseus, where the old single cells are gradually replaced without affecting normal thinking.

Huo Gu, who thought so, did indeed do so.

As the last infected single cell was replaced, the sense of crisis disappeared and was replaced by an incomparable sense of reassurance, after all, what could threaten it was temporarily gone.

Packing up his mood, Huo Gu has not forgotten his current thinking carrier, which is nothing more than an insignificant cluster of single cells, a low-level life form that is even lower than the plants at the bottom of the food chain.

Huo Gu shuddered when he thought that when he was still a human, it had at least a hundred million ways to obliterate the current cluster of single cells.

"It's so fragile, I need to continue to optimize these single cells."

Having established the goal, Hogu began to analyze the unexpected 'surprises', and if the effect of these cell walls was not satisfactory, then it was necessary to perform another operation to completely remove the gene fragments of the phage, and then rebuild the cell wall to meet the needs.

The first requirement is proper permeability, if the permeability is too good, the protective effect will be minimal, if the permeability is too poor, the material that can get the outside world will be less, and the cell will gradually die.

The second requirement is appropriate flexibility, the cell wall can not be too hard or too soft, and there must be a certain degree of ductility, too hard will limit the growth of the cell itself, too soft and can not play a role in buffer protection, if it cannot be extended, the plasticity is also very poor, which is not conducive to holding each other together in today's environment.

The third need, which Hogu urgently needs at the moment, is to be able to defend against the attacks of bacteriophages, aggressive bacteria, and fungi that lurk in the dark.

The first and second requirements are easy to test, just place a single cell in the outermost part of the room, and the rest of the work is just to observe and see if the single cell as the subject of the experiment has changed, and to what extent.

The real difficulty is the third.