Seven hours and three days, there is a huge difference in time, and of course there is also a huge difference in cost, especially when it comes to buying new instruments.

Gu Yanan estimated the scientific research funds for his new project in his heart, and if it was used to purchase a nanopore sequencer, the funds would be tight, so he asked Section Chief Li, "Is the special cost of prevention and epidemic prevention in the laboratory department finished this year?" ”

"Not yet, there are quite a few." Section Chief Li asked, "What are you asking for funds?" Want to buy a new reagent, or a new instrument? ”

"4th Generation Nanopore Sequencer." Gu Yanan gently said the name of the new instrument.

The excitement immediately appeared on Section Chief Li's face, but it faded in an instant, "I have already applied before, but Director Yang of the center thinks that the equipment is too expensive and is rarely used in the monitoring task, so he suppressed my application." ”

The corners of Gu Yanan's mouth curled slightly, and his eyes watched the fluorescence curve on the screen gradually rise, turning into a typical S-shape, and said softly, "Now the opportunity has come." ”

A dialog box popped up on the computer screen, the test was over, and Section Chief Li immediately opened the result analysis.

Ct value 26.

Specimens are strongly positive for Zika virus.

The first imported Zika case in Lianhai City was confirmed.

Section Chief Li understood what Gu Yanan meant, "When the Zika epidemic hit, I need to add new equipment, and I am writing an application on the OA system now, Director Yang, will you tell me?" After all, there is no explicit provision that the genetic sequence of the strain must be obtained within a few days, although the sooner the better, but as a unit leader, the cost will also be considered. ”

"Okay, I'll tell Director Yang, and I'll report the results."

"No problem. Oh, by the way, there is one more thing, even if Director Yang approves the purchase of a new instrument now, it will take a while to go through the process, and there is communication with the instrument manufacturer, all of which will take time, can you use it in time? Don't we have all the strains cultured, the equipment is not in place yet. ”

"Don't worry, I'll deal with the rest of the problems, you can go through the green channel to buy equipment when the epidemic occurs, and the manufacturer's fast delivery should be no problem."

With Gu Yanan's commitment, Section Chief Li was very relieved, "Okay, I believe in Director Gu's ability, you have also participated in countless epidemic treatments, and you have experience, I will give you a report now." ”

After getting Guan Peipei's test report, Gu Yanan first verbally told his brother Gu Jiashu to deal with it accordingly, and then summarized the test results and the previous on-site investigation into a report and handed it to Director Yang of the center.

Inside the office of the Director of the Center.

Director Yang looked at the report submitted by Gu Yanan, his brows furrowed slightly.

This is the first imported Zika case in Lianhai City, and now the Zika epidemic abroad is very serious, and there are imported cases in China, which is very serious, and he has to worry.

"How's it going on over there? Has the mosquito control campaign started in the place where the patient has stayed? Director Yang asked Gu Yanan.

"I have asked Qingcheng County CDC to go to the county hospital, with the place where Guan Peipei stayed as the central point, within a radius of 400 meters to carry out mosquito control, before that was the monitoring point for dengue fever, I just did a Breto index of 10 last week, which is a medium-risk area, but Guan Peipei said that since she got off the plane, she has sprayed toilet water containing effective mosquito repellent ingredients, and has not been bitten by mosquitoes, so it is unlikely to cause the spread of the epidemic."

Director Yang frowned a little, and instructed, "Despite this, this is the first imported Zika case in Lianhai City, we can't take it lightly, all work must be done well, you are responsible for the on-site disposal, and I have to report to the city leaders." ”

Director Yang has already obtained Gu Yanan's report on this imported Zika case, and he is going to deal with it a little more and go to the city to report the situation.

Gu Yanan stood in front of Director Yang's desk, without any intention of leaving.

"Anything else?" Director Yang lifted the reading glasses that he had just brought a few days ago and asked.

"Well, the lab wants to buy a fourth-generation nanopore sequencer, which is just right to sequence the whole gene of the virus on the imported Zika line."

Director Yang took off his reading glasses and looked at Gu Yanan, "Didn't the gene sequencing center you manage just enter a sequencer?" ”

"That is the third generation sequencer, the volume is huge, it takes a long time, now the technology is updated quickly, the fourth generation of nanopore sequencing has come out, the sequencing time is very short, from the collection of samples to the gene sequence, it only takes 7 hours, before the one to do sequencing must first isolate the virus strain, design primers to synthesize primer amplification fragments before it can be carried out, plus the time of virus culture at least three days or more.

This fourth-generation nanopore sequencer can meet the handling of sudden epidemics, get results quickly, and the size is very small, which can be brought to the flow control site.

Now that a certain virus or bacteria is the causative agent of a certain disease, it is the gold standard to get the bacterial strain, so the third-generation sequencer we have bought and the fourth-generation nanopore sequencer that we are about to buy have their own roles. ”

Director Yang is also an inspector and knows the importance of new inspection technology to deal with the epidemic, and he also believes in Gu Yanan, so he nodded and agreed, "Okay, then write an application for instrument purchase on the OA system, and I will make another report to the city, take the green channel for instrument purchase during the epidemic, and try to make it possible for you to deal with this Zika epidemic." ”

As Gu Yanan expected, Director Yang really agreed.

In terms of emergency response to the epidemic and scientific research, Director Yang has always been unambiguous.

"Okay, thank you Director Yang, I will send the manufacturer and configuration parameters of the instrument to your mailbox in a moment."

From the office of Director Yang of the center, return to the emergency office.

Several members of the emergency response team are there.

Seeing Gu Yanan coming back, Xiao Chu greeted him and asked, "Sister Yanan, have Guan Peipei's test results come out?" ”

"It's out, PCR confirmed positive for Zika virus."

Because Xiao Chu had already told the members of the emergency team about the situation in the hospital before, the results fed back by Gu Yanan were the same as everyone expected, so no one was surprised.

Zhou Lao asked Gu Yanan, "Do you want to go over to guide the scene in Qingcheng County?" ”

Gu Yanan was about to say this, "Someone should guide the mosquito control work on the spot, Old Zhou, this matter will be handed over to you." ”

"No problem, I'll go to Qingcheng County now, where I supervised the density of Aedes aegypti mosquitoes and rodent density monitoring in the past." Zhou Lao began to pack his things as he spoke, ready to go.

Usually goes to the county to guide, usually he is in a group with Zhou Lao, but now only Zhou Lao is allowed to go alone, Gao Fei is a little anxious, "Is it okay to be alone?" What about me? ”

Gu Yanan: "Zhou Lao used to just guide, and the on-site mosquito control work was done by the people of Qingcheng County Disease Control, and I have other things for you to do." ”