"Stem cells?" After listening to Junxin's words, everyone looked at each other.

"With all due respect, sir!" After listening to Jun Xin's words, Professor Nat couldn't help but say, "The stem cell experiment is quite complicated, are you really ready for this experiment?" ”

"I just prepared the project, of course, it has nothing to do with my teaching course, it's just my own interest!" Junxin explained, "What I am doing should only be a small branch of stem cells, and there will not be too many complicated things in it. ”

"But I have to remind you that a complete stem cell can take a long time, and because of the special nature of stem cells, experiments on stem cells require ethical certification before they can be carried out, and you must have a good understanding of policy issues."

"I'm sorry, maybe I wasn't talking enough!" Jun Xin smiled apologetically and said, "The stem cell experiments I did will avoid ethical scrutiny, which means that my stem cell experiments will not be in the scope of ethics." ”

"You mean, pluripotent stem cell IPS?" After listening to Junxin's words, as a professional in stem cell research, Clement Shashefsky's eyes lit up and asked urgently.

Junxin smiled and nodded. In fact, stem cell research is also divided into many types, and the source of early stem cell research is primary stem cells isolated from animals. In modern times, due to the development of science and technology and the progress of some medical technologies, the sources of stem cells tend to be diversified, not only from the isolation and culture of animals, but also from the isolation of stem cells from the human body.

As modern ethics is gradually embodied in modern medical and biological experiments, the ethical review of biological experiments in European and American countries is becoming more and more intense. Now, cells of both animal and human origin are under intense scrutiny. This caused problems with the progress of the experiment. According to the classification of ethical review, the types of stem cell research can be divided into several categories. For example, embryonic stem cells, neural stem cells, etc., have become strictly controlled experimental cells. If you are studying its primary cells, it is even more of a headache. Because under ethical scrutiny, these cells have basically no source. The reason why Professor Nat is worried is that he thinks that Junxin is engaged in the research of these primary stem cells.

The so-called pluripotent stem cells can be induced to differentiate into any other cell, but they lose the ability to develop into a complete individual, and their developmental potential is limited to a certain extent. Bone marrow pluripotent hematopoietic stem cells are a typical example that can differentiate into at least twelve types of blood cells, but not cells outside the hematopoietic system.

"What do you want to do?" "You know, one of the most common ideas in the academic community is that pluripotent stem cells are still derived from embryonic stem cells. It is also a type of stem cell experimental research that cannot avoid ethical scrutiny. ”

"I know, but the direction of my experiment may be a little different than what you think." Jun Xin is very familiar with Shashewsky's words, but the views that people generally believe now do not mean that Jun Xin must look at the problem according to their point of view. To be honest, Junxin looks at problems from the perspective of the future. So what he is looking at is not how to use ready-made pluripotent stem cells for research, but how to reverse the way around, how to turn ordinary cells into pluripotent stem cells through induction.

"I won't say much about what the nature of stem cells looks like. As a student and assistant to Professor Andrey, you know a lot better than I do. But what I'm saying is, why don't you think about it in a different direction? We've always focused on how stem cells are induced to transform into specific other cells, but on the other hand, why not consider whether other cells can be transformed into stem cells? We know that every cell has the potential to be totipotent! Junxin said. I didn't care about his remarks, which completely suppressed the experimenters in the laboratory.

With this next experimental design, as long as you have a certain experimental foundation and experience, plus an understanding of the operating principles and analysis procedures of various instruments, then a fresh result can come out. Far from it, let's just say that graduate students, after getting the project, most of them are equivalent to getting an ideal, followed by the design plan, design process, experiment and then organize the data, and finally become the results and papers.

Of course, there are cases where even if you know the subject but don't know how to proceed, and this is also the norm in scientific research.

In fact, after Junxin redesigned the research direction of the laboratory, although this ideal is very remarkable, it is not easy to achieve this goal. At the very least, how to design this topic, how to verify it, and what methods to use to achieve it is not a simple matter, and even this will be a key part of this experiment.

At this time, how to solve these problems and how to reasonably allocate the resources of the laboratory to achieve this goal can test the ability of the controller of a laboratory.

"Klein, I need you to search for relevant information in the early stage of the experiment, find cells with different degrees of differentiation, not at the same level, and expand into primary cell lines through culture." Junxin began to assign tasks.

"No problem, I'll have all the thirty or so cell lines ready in half a month."

"Okay, but I ask you to provide two, one is fibroblasts and one is hepatocytes. You know better than I do the characteristics of these two cells, no problem, right? ”

"Nope!"

"Michigan Green, you've been assisting Klein in this mission lately, mostly outside of your research. In addition, I need you to be proficient in using flow cytometry. ”

"Yes, sir!"

"Navel, your project is almost over, this time to concentrate on your final thesis defense, of course, if you have time, I hope you can help design an enzyme-linked immunoassay for the supernatant of the cell culture medium of stem cells, which involves a large number of antibodies, I will let Shirley assist you, no problem, right?"

"We're here to help!"

"No problem!"

"Hurt, your task is relatively heavy, I need you to verify and find out the genes related to stem cells in the known information, and give me the general information after sorting it out, a month's time, is it enough?"

"I'll try my best!"

"I'll ask a few other graduate students to collect it for you, and I'll apply for the library's journal papers to be open to you, a month is a bit heavy, but I hope you can do your best."

"Yes, sir!"

"Mr. Nat, you are responsible for supervising and following up the progress of the experiment, and the stem cells left over from the original Professor Andre will continue to be cultured for verification experiments."

"No problem, sir!"

"Half a month later, we are still in the same place, for the next meeting. If there is any problem, you can directly raise it with Professor Nut, and I will help you solve it as soon as possible after telling me. Meeting! ”

"Yes, sir!"