Yang Zhou felt that his body was getting weaker and weaker, and he would be out of breath after walking a few steps, and he didn't know if it was the cause of the spread of cancer.

The state of his body made Yang Zhou very clear that he could not be delayed by other things.

Yang Zhou's affairs of the Oasis Ecological Company were all handed over to Zhong Hong to deal with.

The largest order has been completed, with more than 4 million final payments received, and more than 2.9 million remaining after deducting all costs.

Yang Zhou used the best drugs for chemotherapy, which probably cost more than 40,000 yuan, and these medical expenses did not cost Tang Yue's money, but were transferred to the hospital's public account with the company.

Tang Yue also knew that Yang Zhou had opened a company outside the school, and now that he had money, he began to help Yang Zhou find office space in Zhongguancun in his free time.

The school's charity activities are now completely in charge of Lu Yuyao, Yang Zhou talked to her in detail, and simply handed over the online store to her to operate, and he wanted to put all his mind on the experiment.

Although Lu Yuyao was a little unhappy, things had come to this point, so she had to agree.

After all, the whole thing was initiated by Yang Zhou, and there is nothing to blame for him relying on this activity to operate several online stores, and many of the network resources used in the whole thing are also Yang Zhou's matchmaking.

Lu Yuyao began to contact Guo Wentao alone, and the two sides communicated about publicity.

Yang Zhou returned to the laboratory and officially did experiments and wrote papers.

There is still a lot of preparation work in the early stage, first of all, let the carrot seeds absorb water and swell.

Seed development is divided into several stages, the first stage is water absorption.

At this stage, the hydrophilic substances inside the seeds attract water molecules, and the strength of the seeds' expansion can even break through the glass and lift boulders.

The protagonist of some novels pretends to be a ghost to make the Buddha statue grow automatically, which is the principle of using seeds to absorb water and expand and germinate.

After the water absorption is over, the inside of the seed changes dramatically, the gel state changes to the sol state, various enzymes begin to activate, and the respiration and metabolism are sharply enhanced.

For example, after barley seeds swell, the embryo first releases gibberellin and transfers it to the aleurone layer, where it induces the synthesis of hydrolase enzymes (α-amylase, protease, etc.).

Hydrolase hydrolyzes the starch and protein stored in the endosperm into soluble substances (maltose, glucose, amino acids, etc.), such as maltose, which are fermented by seeds.

Yang Zhou is now going to operate at this stage.

The genetic material of the carrot seed must be altered at this stage, otherwise shoots will grow, which is equivalent to the genetic material has taken effect, the DNA has spread to each cell, and it is no longer possible to interfere with plant growth unless minor surgery is performed on the whole plant.

Today's experimental equipment has been able to insert needles into cells.

As long as the edited bacterial clipping tool is sent to the germ cells of radish seeds, it can automatically find the corresponding clip gene for change.

It took a day for Yang Zhou to put all the dozens of seeds into the nutrient solution.

This allows the seeds to absorb enough nutrients more quickly to enter the second stage.

When it came to the transformation, the equipment in Bai Shulong's laboratory was insufficient, and Yang Zhou could only go to the microbiology laboratory to borrow their confocal microscope.

Under the observation of this microscope, the cells can be manipulated with other instruments.

Today's highest-end experimental instruments are capable of controlling atoms and arranging them into animations.

And more than 20 years ago, when cloning was the most popular, scientists were able to control experimental instruments to clone animal cells, and genetically modified plants also appeared during that time, all of which are actually related to the progress of experimental instruments.

There are a lot of people in the microbiology laboratory, and probably because of the catastrophe of '19, various universities pay special attention to the microbiology major.

Among the master's and doctoral students of Qingbei University, there are many people majoring in microbiology.

Many of these people were poached by the school from other universities or even abroad, especially now that the international situation is tense, and countless students studying abroad have returned to China.

When Yang Zhou came to the microbiology laboratory, he couldn't mix with experimental instruments at all.

But his seeds have enough time to absorb water, and if they are allowed to ferment, they will soon germinate and lose their effect.

The only resort is to seek help from a mentor.

"Teacher, I've already arrived at the microbiology lab, but the machine has been operated, I asked, and it was a few days later, but my seeds can't wait." Yang Zhou said on the phone.

Bai Shulong helped Yang Zhou contact the experimental equipment, and he just tried to line up with others.

He immediately said: "Then you can line up now, after all, we are borrowing someone else's equipment, anyway, the seeds are not worth a few dollars." ”

Yang Zhou naturally knew that the teacher was right, but he really couldn't delay, and he would have chemotherapy again in a few days.

The experimental procedure he did today was just the beginning, because in order for the gene clipper to take effect, the precursor DNA had to be obtained in advance, and if he wanted to edit that segment, he had to obtain that piece of DNA, and then modify the editor made by bacteria.

In the next few days, Yang Zhou had to use experimental equipment many times, and he couldn't queue up for experiments like other students.

Yang Zhou could only ask the teacher for help.

Bai Shulong really cares about his students, and waited for a long time before calling Yang Zhou and said: "Okay, I got you 2 hours a day, but you have to keep a low profile, this time it takes up the time of many of their students, the professor won't say anything, and the students must have some opinions about you." ”

"Thank you, teacher!" Yang Zhou hurriedly thanked.

He doesn't care about offending students, even if he is a professor, if he offends, he will be offended, and now his life matters.

If it weren't for the lack of money, I really wanted to buy such an experimental instrument, but 5 million US dollars is too expensive, and there are not many such equipment in China.

Qingbei University can't do it, only to go to Shuimu University of the same level, or go to the laboratory of the Chinese Academy of Sciences, but everyone is a big project, and it is impossible for Yang Zhou to go to the laboratory of the Chinese Academy of Sciences as a student.

Private laboratories rarely have such top-of-the-line equipment, but the laboratories of several vaccine companies certainly have such equipment.

Because of well-known reasons, several vaccine companies are operating at full capacity, and Yang Zhou has no relationship in this regard, so he can only squeeze out the time of other students and use the experimental equipment of Qingbei University.

The time reserved for Yang Zhou was an hour later, which was supposed to be arranged after the early hours of the morning, but Yang Zhou arrived at the scene, and the seeds were also prepared, and under the operation of Bai Shulong, it was determined that the equipment could be borrowed in the afternoon.

As a four-year-old experimental dog in the Department of Biology, he is no stranger to the equipment of the microbiology laboratory, knowing that Yang Zhou is going to do the experiment, the professor of the microbiology laboratory does not need to remind Yang Zhou how to operate the equipment.

There was only one hour to prepare, and Yang Zhou had to hurry up to apply for equipment.

The use of confocal microscope is only able to see the three-dimensional structure of the cell, and the 3D structure of the cell can be roughly photographed on the monitor, and the specific operation also requires the embryo's fluorescence stereoscope, a full set of microinjection, fluorescence in vivo imaging and other messy experimental equipment.

Only the microbiology laboratory is so complete, and it is said that the equipment of the entire laboratory cost 240 million yuan.

To use the equipment, you have to fill in the application form, and with the consent of the boss of the microbiology laboratory, the postdoctoral fellows who are responsible for the management below will naturally not be embarrassed by Yang Zhou.

However, Yang Zhou robbed others of their experimental time, and he was not less blind along the way.

Especially the returnee master's and doctoral students who are about to do the experiment, followed Yang Zhou throughout the whole process, and saw Yang Zhou writing the carrot Cas9 gene editing experiment on the form, and his emotions could not be suppressed.

Gene editing is a high-end project in China, and students like Yang Zhou basically don't do this kind of experiment.

"Hehe, I thought it was some kind of experiment, it turned out to be a gene clip, the boss is still too kind, and he actually asked you to borrow equipment, what a waste of everyone's time!" Peng Zhenqiang scoffed.

Yang Zhou raised his head, glanced at the student who was speaking, and continued to bury his head in writing the form, he didn't bother to pay attention to this kind of person.