Chapter 32: Beginning the Experiment (Asking for Support)
The more you ignore someone, the more he goes too far.
Peng Zhenqiang thought that when he talked about Yang Zhou's sore spots, he intensified his ridicule, Yang Zhou ignored it, and he chatted with other postdoctoral fellows and master's and doctoral students present.
The words were full of ridicule for Yang Zhou occupying the pit and not.
And it was concluded that Yang Zhou would definitely fail.
After all, gene editing is a new technology, and its application abroad is not yet mature.
If it was really mature, countless gene-edited plants would have appeared in the world long ago, and Huaguo would not have been manipulated by foreign companies because of genetically modified soybeans.
As long as a certain technology is mastered by the Chinese, the price of this technology will plummet.
But nowadays, whether it is the Cas9 editor used for gene editing or the seeds of gene editing, they are very rare on the market.
The most fundamental reason is that the experiment is too difficult.
Peng Zhenqiang carefully read Yang Zhou's experimental description, Yang Zhou wanted to transform the carrot, control the color of the carrot, and far exceed the size of the current radish.
In his opinion, Yang Zhou is simply impossible.
Then he occupies the equipment of the microbiology laboratory, which is a waste of everyone's time.
In particular, it is still his experimental time that is taken up!
You must know that it is not easy for everyone to use the instrument once, and Yang Zhou still has to occupy 2 hours a day, which is simply intolerable.
Peng Zhenqiang planned to keep taunting Yang Zhou, and then make him embarrassed to come over for an experiment.
It's a pity that Yang Zhou didn't enter the oil and salt, and didn't pay attention to him at all.
Peng Zhenqiang didn't dare to tell the boss that his decision was wrong, after all, the boss had already promised Yang Zhou to use the equipment, and there must be Boss Yang Zhou behind him.
He didn't have the ability to stand up to the professor boss behind Yang Zhou.
It was Yang Zhou's turn to do the experiment, he changed into experimental clothes, and Tang Yue also rushed over at this time.
More than a dozen people in the microbiology laboratory suddenly lit up when they saw a big beauty like Tang Yue, and several boys stepped forward to ask Tang Yue if he needed help.
Peng Zhenqiang thinks that he is charismatic, after all, he once studied in graduate school at an Ivy League university, but because of the epidemic, he did not get a graduation certificate, and finally chose to continue his studies at Qingbei University.
"Classmate, are you going to do any experiments?" Peng Zhenqiang put on a smile that he thought was a gentleman and said slowly.
Tang Yue's eyes searched in the laboratory, but he didn't see Yang Zhou, and when he saw that a senior took the initiative to talk, he asked, "Do you know which room Yang Zhou is doing experiments?" I was his lab assistant. ”
Suddenly, the whole laboratory fell silent, and many students stopped paying attention to Tang Yue and began to busy their own business.
Only Peng Zhenqiang looked at Tang Yue with a cloudy face.
"Senior?" Tang Yue shouted again.
"Oh, you said the guy who did the gene editing, he found it himself." Peng Zhenqiang waved his hand happily and said.
After speaking, he ignored Tang Yue, shook his head and went to his own business.
He really couldn't figure out how Yang Zhou could have such a beautiful experimental assistant.
I was also shouting in my heart, why couldn't I meet such a good-looking assistant, as we all know, Qingbei University is a top university, and there are really not many beautiful girls.
After all, when a beautiful girl was in high school, she was harassed by her male classmates in various ways, and the probability of good grades was not high.
Sighing and returning to his own territory, Peng Zhenqiang still couldn't calm down in his heart, and decided to continue to observe Yang Zhou, if he was really a trash, then don't blame him for being unkind.
Yang Zhou entered the experimental room of the high-end instrument, took out all the prepared carrot seeds, marked them, and then checked the status of the equipment.
Today's task is to take what you have in mind and use the simulation experiment in the real world.
In fact, by using the genetic modification function of the mysterious space, you can get the modified carrot seeds immediately.
But take out the seeds out of thin air, how can you write a thesis?
If you want to publish a paper in an international journal, you must at least allow other laboratories to repeat the experiment, otherwise the suspicion of paper falsification cannot be ruled out.
Yang Zhou naturally wanted to make special radish seeds repeatedly in the real laboratory.
There are still many challenges that need to be solved.
For example, in the simulated experimental space, Yang Zhou easily found relevant DNA fragments and modified them for sealing.
In reality, it's not that easy.
After finishing the preliminary preparations, Tang Yue also walked into the laboratory in a lab coat.
"Senior brother, I'm here, what do you need me to do?" Tang Yue asked energetically.
Yang Zhou was fiddling with the instrument, and said without raising his head: "You come to make experimental records, we only have 2 hours, the experimental records are ready, and we will take things away later." ”
"Okay!" Tang Yue nodded.
She also often records experiments in Bai Shulong's laboratory, and she is already familiar with these things.
Soon she began to record the number of the petri dish prepared by Yang Zhou in the experiment book, what equipment Yang Zhou used, what steps he did, all of which had to be recorded, even accurate to a few minutes and seconds.
Yang Zhou dissected the carrot seeds to reveal the fetal buds inside, then placed them on a glass sheet and finally placed them in a confocal microscope.
Shortly after starting the instrument, a 3D image appears on the computer screen.
Yang Zhou struggled to identify the 3D structure diagram of the computer screen, and then compared it with the clear 3D structure diagram in his mind to find familiar places.
But Yang Zhou watched it for about half an hour, and even Tang Yue had already yawned, and he still didn't find the DNA fragment he wanted.
"Senior brother, are you in trouble?" Tang Yue saw Yang Zhou stop moving, and immediately stepped forward to care.
Yang Zhou rubbed his swollen temples and nodded slightly.
The situation was much more difficult than he had imagined, and the genetic fragments that could be easily found in simulated experiments in the mysterious space were very difficult in reality.
The first shot is very blurry, and then the genes themselves are very small, all gathered together, carrots have more than 30,000 base pairs, Yang Zhou needs to find one of them, and there are no external characteristics, which is like looking for a needle in a haystack.
"It's very difficult, gene editing is rarely used in agriculture now, not without reason, we need to find the corresponding genes, and then accurately extract them, and then inject them into the Cas9 editor with a microsyringe, but the problem is that I can't find .......," Yang Zhou muttered.
Tang Yue is not a scientific research idiot, she is also a biology college student, listening to Yang Zhou's words, her mind is still very confused at this time.
"How can Senior Brother determine the function of DNA?" Tang Yue wondered.
Without waiting for Yang Zhou to explain, she said according to her own understanding, "There are more than 30,000 genes in carrots, and if we want to figure out the specific role of genes, we need to extract 30,000 genetic tissues, then modify 30,000 gene editors, and then cultivate 30,000 radishes." ”
She couldn't go on anymore, because the first step would be stuck, how to accurately extract 30,000 different gene fragments.
When extracting cell tissue, it is likely that everything is a large piece, and it is impossible to be precise at all.
It needs to be extracted 30,000 times accurately, and maybe hundreds of thousands of experiments need to be completed accurately.
And every time you want to know the results of the experiment, you have to wait for the carrot to mature, even if hundreds of thousands of experiments are carried out at the same time, it will take more than 3 months to know the answer.
This is where Yang Zhou's experiment is the most difficult.
Yang Zhou couldn't tell Tang Yue that he already knew the specific genetic information contained in each fragment of carrot DNA, so he just said vaguely, "So, we are also lucky, we will do 1,000 experiments in a week, and if we are lucky, we may just get the desired result, God may have a lifetime of luck for the sake of death." ”
Tang Yue's expression was complicated when he heard this, and he didn't know whether to laugh or cry.
In the end, he smiled and encouraged: "En, that's right, God will also help us, senior brother will definitely succeed!" Come on! ”
He waved his little fist as he spoke.