Wu Zhe flashed the information he had just read, and he had a little idea in his mind. Look up and reply:

"Well, I have some ideas, but there are still too few materials and data, and I need Senior Brother Zheng to help me get the data together."

Tang Meng's spirit couldn't help but be shocked, she knew that Wu Zhe was not a person who opened her mouth, and hurriedly said:

"Actually, you can speak directly, Senior Brother Zheng is a good person, and he still takes good care of us juniors."

Wu Zhe nodded: "Well, I see that he is good, otherwise I wouldn't really want to help him lose weight." I really like to draw pictures when I'm fine! ”

"Tell me, then, what do you think?" Tang Meng showed a curious look. Wu Zhe has only been here for a morning, and he has made a discovery. She also wants to hear Wu Zhe's thoughts, scientific research is not afraid of mistakes, but afraid of no direction.

Wu Zhe smiled and said: "It's easy to say, maybe it can't be realized?" I think there are two aspects that can be started, one is to replace the algorithm of the current statistical data, and start from the mathematical side to see if it can be optimized. The second is to consider whether the previous sequencing method can be replaced. ”

Tang Meng also nodded again and again after hearing this. said to Wu Zhe: "The first of these two methods is still possible, but the second is difficult, and you should also know the current sequencing standards, both methods are methods more than 20 years ago." After so many years, no one has ever come up with a new theory. ”

Today's gene sequencing methods are still the chain termination method pioneered by Sanger and Colson in the 70s or the chemical method (chain degradation) invented by Maxim and Gilbert.

The core principle of the Sanger method is that since the 2' and 3' of ddNTP (4 fluorescently labeled A, C, G, T bases) do not contain hydroxyl groups, they cannot form phosphodiester bonds during DNA synthesis, so they can be used to interrupt the DNA synthesis reaction. A certain proportion of ddNTPs with radioisotope labels (ddATP, ddCTP, ddGTP and ddTTP) were added to each of the four DNA synthesis reaction systems. Then, after gel electrophoresis and autoradiography, the DNA sequence of the molecule to be tested can be determined based on the position of the electrophoresis band.

This method is now the mainstream mapping method, and the core methods such as pyrosequencing and ligase methods that have emerged later all use dNTPs in Sanger that can interrupt the DNA synthesis reaction. Therefore, the current means are basically inseparable from the Sanger method.

That's why Tang Meng said that it would be very difficult for Wu Zhe to create a new surveying and mapping method.

Wu Zhe smiled and said: "I just have such an idea, let's start with the Sanger method and see if the data calculation can be improved!" Then I'm going to try out the Sanger method myself, so that people don't think that I can't even get started after I enter the genetic institute. ”

"It's simple, it's annoying to worry about gene comparison and reading, and the operation of the Sanger method is basically what everyone in the institute will do. Why don't I teach you? Tang Mengjiao looked at Wu Zhe with a smile, it was rare to meet Wu Zhe.

"Why are you looking at me like that? I haven't done it again, of course not. You really think I can do anything! Wu Zhe looked at Tang Meng and couldn't help but pinch her nose gently.

Tang Meng had classes in the afternoon, and he went back to class before class. Wu Zhe began to study the previous experimental data. Then, I put all the experimental data in the past two years into my head, and began to deduce the commonalities and differences of these data in my brain. I also picked up the scratch paper and began to write, write, draw, and draw.

When Zheng Yizhong returned to the office, he looked at the scratch paper that had been laid out on the table. opened his mouth and asked Wu Zhe: "I just left for a few hours, Ah Zhe, what are you going to do?" ”

Wu Zhe didn't raise his head and said, "Senior Brother Zheng, did you buy Chinese medicine?" I'll see if I can optimize the algorithm. Then see if you can rewrite a calculation program? ”

Zheng Yizhong immediately put down the things in his hand, walked to Wu Zhe's side and said, "Can you do it?" If that happens, it's a big credit, and the most important thing right now is time. ”

Then he looked at the mathematical algorithms and models written by Wu Zhe. However, he looked lonely. The Latin symbols on this paper are still known one by one, but they are connected together, and they are almost the same as Taoist talismans.

Seeing that Zheng Yizhong had been studying, Wu Zhe couldn't help but ask, "Senior brother, is there a problem with me above?"

Grains

As soon as Wu Zhe spoke, Zheng Yizhong's hand holding the paper shook, and then he cleared his throat and said, "Senior brother, you are asking the right person, there is a big problem here." ”

Wu Zhe was also shocked when he heard this, and couldn't help but put down his pen and looked up at Zheng Yizhong who was standing in front of him. The expression was also puzzled: "Is there something wrong?" You're just the beginning. You can't go wrong! ”

"Hey, hey, don't worry! I didn't say you're wrong, what I want to say is that you do math calculations, why don't you have a few numbers, and the paper is full of letters? Zheng Yizhong asked embarrassedly.

Wu Zhe breathed a sigh of relief, and then rolled his eyes at Zheng Yizhong. You can't even understand it, and you're still carrying it around for a long time. Ignore him and continue to bury his head in calculations.

Is math good as a cow? Yes, this time a freshman was despised by a freshman. But it really can't be helped. People who are good at math are really awesome, and if they can't get up, they really can't. This math is followed by more symbols than numbers.

When Tang Meng got out of school, Wu Zhe was still modeling. This is much more difficult than in the Mathematical Modeling Contest for College Students.

Tang Meng's mathematics is obviously passable. Flipping through Wu Zhe's calculation paper, I also roughly understand Wu Zhe's thinking. According to Wu Zhe's ideas, the comparison time can be increased by one-third, don't think that this data is not good. That's a big step forward.

After knowing Wu Zhe's train of thought, Tang Meng also joined in. The two partnered, and the progress was much faster.

It was also slowly getting dark.

Zheng Yizhong said to the two, "Have you gone back?" I'll go first. ”

Wu Zhe replied with a smile: "Senior brother, let's go first, don't forget to take that Chinese medicine, and see the effect in a few days." Do I want you to help me? ”

"That's not what you say, as long as it works. I will certainly keep my word. "Alright, I'll go first.

Wu Zhe and Tang Meng were not very late, as long as Wu Zhe didn't want Tang Meng to accompany her hungry. Besides, the data model of the calculus has passed his mathematical calculus. Then it's time to program.

"Mengmeng, let's go back too! It's definitely not going to be good for a while. Wu Zhe said to Tang Meng, who was still writing the arithmetic.

"Okay! Wait until I'm done writing here. After Tang Meng finished writing, he handed the part he wrote to Wu Zhe.

Wu Zhe picked it up and took a quick look at it. Then praised: "It's really worthy of being able to suppress a person in high school, this level is not low!" ”

Tang Meng replied with a look that you only knew.

Wu Zhe smiled and told Tang Meng about what happened when Zheng Yizhong looked at the calculations on the scratch paper in the afternoon.

"I thought Senior Brother Zheng was a master, and I was afraid that I had miscalculated, so I looked at him cautiously, and he gave me a sentence: There are too many symbols and too few numbers. I almost didn't hold on. ”

When Tang Meng heard this, he also "giggled" and laughed.

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